Re-used progesterone devices efficiently synchronise oestrus and ovulation after autoclaving process in Toggenburg goats during the breeding season

2015 ◽  
Vol 55 (6) ◽  
pp. 818 ◽  
Author(s):  
J. M. G. Souza-Fabjan ◽  
C. A. A. Torres ◽  
A. L. R. S. Maia ◽  
F. Z. Brandão ◽  
E. Oba ◽  
...  
Keyword(s):  
Breeding Season ◽  
Corpora Lutea ◽  
Device Removal ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Follicle Diameter ◽  
Lactating Females ◽  
Equine Chorionic Gonadotropin

This study compared new and previously used (and autoclaved) progesterone devices for synchronisation of oestrus during the breeding season in Toggenburg goats. Nulliparous (n = 17) or lactating (n = 50) received new intravaginal devices containing 0.3 g progesterone (CONTROL), or similar devices previously used for either 6 (USED6) or 12 (USED12) days and subsequently autoclaved. All goats received 5 mg dinoprost at device insertion and 200 IU equine chorionic gonadotropin 5 days later and all devices were removed after 6 days. After device removal, females were mated by fertile bucks. Ovarian ultrasonography was performed every 12 h after device removal until ovulation detection. Blood samples were collected for determination of plasma progesterone concentration in different moments and intervals (from 7 days before device insertion to 3 days after its removal). There was no difference (P > 0.05) among groups CONTROL, USED6 or USED12 for: oestrus response [75% (18/24), 77% (17/22) or 71% (15/21), respectively]; duration of oestrus (30.7 ± 3.4, 31.8 ± 1.7 or 32.8 ± 3.4 h), percentage of ovulating goats [67% (6/9), 78% (7/9) or 56% (5/9)], ovulation rate (1.3 ± 0.2, 1.4 ± 0.2 or 1.8 ± 0.4 units of corpora lutea), average follicle diameter (6.2 ± 0.1, 6.7 ± 0.1 or 6.8 ± 0.3 mm) and pregnancy rate [54% (13/24), 50% (11/22) or 48% (10/21)]. Plasma progesterone concentrations were not different (P > 0.05) for does among treatments and between nulliparous and lactating females. In conclusion, autoclaved, previously used intravaginal progesterone devices are effective in synchronising oestrus and ovulation in cyclic goats during the breeding season.

scholarly journals Energy supplementation in Santa Inês sheep subjected to estrus induction treatment

2016 ◽  
Vol 37 (1) ◽  
pp. 145 ◽  
Author(s):  
Sabrina Silva Venturi ◽  
Jeferson Ferreira da Fonseca ◽  
Ana Clara Sarzedas Ribeiro ◽  
Marcos Claudio Pinheiro Rogério ◽  
Juliana De Oliveira ◽  
...  
Keyword(s):  
Induction Treatment ◽  
Dietary Energy ◽  
Reproductive Parameters ◽  
Device Removal ◽  
Short Term ◽  
Blood Samples ◽  
Follicle Diameter ◽  
Santa Inês ◽  
Energy Supplementation

The aim of this study was to evaluate the effect of a 20% increase in dietary energy during short-term estrus induction treatment on the reproductive parameters of Santa Inês ewes. Females (n=43) were allocated into two experimental groups according to the amount of energy inclusion in the diet: maintenance diet or maintenance diet plus 20% energy. Ultrasound examinations were performed in order to detect ovulation. To assess sexual behavior, ewes were teased and further mated. Blood samples were collected for the determination of glucose and insulin concentrations. There was no difference (P>0.05) between groups in the following categories: ovulation rate (80.00% vs. 60.00%), largest follicle diameter (6.00 ± 0.20 vs. 5.90 ± 0.60), interval from device removal to ovulation (52.80 ± 14.87 vs. 59.01 ± 8.34 hours), animals in estrus (75.00% vs. 65.21%), interval from device removal to estrus (30.00 ± 15.49 vs. 30.00 ± 13.35 hours) and conception rate (50.00% vs. 21.73%). There were differences (P<0.05) in the concentrations of insulin and glucose. It can be concluded that the 20% increase in energy in the diet during short-term estrus induction treatment did not affect the reproductive parameters studied.

scholarly journals The use of Cloprostenol and prostaglandin F2alpha to induce luteolysis in reindeer calves (Rangifer tarandus)

Rangifer ◽  
1991 ◽  
Vol 11 (2) ◽  
pp. 13 ◽  
Author(s):  
Erik Ropstad ◽  
Dag Lenvik
Keyword(s):  
Breeding Season ◽  
Rangifer Tarandus ◽  
Prostaglandin F2alpha ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Treatment Groups ◽  
Significant Fall

A total of 126 reindeer of about 7 months of age, were isolated from a flock at the end of the breeding season. The animals were treated either with 12.5 mg prostaglandin F2alpha (n = 41) or 0.25 mg cloprostenol (n = 50). Thirty-five animals were left untreated. Blood samples were collected before treatment and 2 Vi days later and the plasma progesterone concentrations were determined. A significant fall in progesterone concentration was seen in both treatment groups. A large proportion of animals responded to treatment with cloprostenol than with prostaglandin F2alpha. It was concluded that prostaglandins can be used to induce luteolysis in reindeer.

A note on attainment of puberty of september-born early-maturing ewe lambs in relation to level of nutrition

1991 ◽  
Vol 53 (3) ◽  
pp. 407-409 ◽  
Author(s):  
F. Forcada ◽  
J. A. Abecia ◽  
L. Zarazaga
Keyword(s):  
Sexual Activity ◽  
Breeding Season ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Plasma Progesterone ◽  
Ewe Lambs ◽  
Early Maturing ◽  
Age At Puberty

The attainment of puberty in September-born early-maturing ewe lambs was studied at Zaragoza (latitude 41° 40' N). Thirty twin Salz females were allocated to two groups receiving two nutrition levels after 3 months of age: high (500 g/day lucerne hay and 500 g/day concentrate) (H) and low (500 g/ day lucerne hay) (L). Oestrus was detected daily by aproned rams. Corpora lutea were counted after oestrus and plasma progesterone levels monitored each week.In the first breeding season (January to February) the percentage of females showing sexual activity (silent emulation or oestrus and ovulation) was higher in the H compared with the L group (67 and 20%; P < 0/05). Nonpubertal oestrus before the main breeding season was detected in 67% of animals. In the main breeding season and for H and L groups respectively, percentage of females showing silent ovulation before puberty was 67 and 33% and mean age at puberty extended to 319 (s.e. 4-8) and 314 (s.e. 3·7) days. Ovulation rate at puberty was 1·73 (s.e. 0·13) and 1·33 (s.e. 0·15) respectively (P < 0·05).

scholarly journals Ovarian follicular dynamic and plasma progesterone concentration in Alpine goats on the breeding season

2018 ◽  
Vol 70 (6) ◽  
pp. 2017-2022 ◽  
Author(s):  
N.G. Alves ◽  
C.A.A. Torres ◽  
J.D. Guimarães ◽  
E.A. Moraes ◽  
P.B. Costa ◽  
...  
Keyword(s):  
Breeding Season ◽  
Progesterone Concentration ◽  
Plasma Progesterone

Efficiency of mating, artificial insemination or resynchronisation at different times after first timed artificial insemination in postpartum Nellore cows to produce crossbred calves

2019 ◽  
Vol 59 (2) ◽  
pp. 225 ◽  
Author(s):  
Walvonvitis Baes Rodrigues ◽  
Jean do Prado Jara ◽  
Juliana Correa Borges ◽  
Luiz Orcirio Fialho de Oliveira ◽  
Urbano Pinto Gomes de Abreu ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Beef Cows ◽  
Device Removal ◽  
Oestradiol Benzoate ◽  
Timed Artificial Insemination ◽  
Equine Chorionic Gonadotropin

The objective of this trial was to evaluate different post-timed artificial insemination (TAI) reproductive managements in postpartum beef cows to produce crossbred calves from artificial insemination (AI). Nellore cows (n = 607), with 45 days postpartum, were inseminated at a fixed time, using a protocol that included an intravaginal progesterone-releasing device along with oestradiol benzoate, prostaglandin, equine chorionic gonadotropin, and oestradiol cypionate, followed TAI 48 h post-device removal. Four post-TAI treatments were evaluated: in CONTROL (T1, n = 161), cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). In OBSERVATION (T2, n = 132), heat detection was performed for 15–25 days post-TAI, followed by AI. In RESYNC22 (T3, n = 157) and RESYNC30 (T4, n = 157), resynchronisation started after 22 or 30 days, following second TAI at Day 32 or 40 days after first TAI. In T2, T3 and T4, after the second AI, cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). The pregnancy rate (PR) for the first TAI did not differ (54.6%, 53.0%, 59.2%, and 51.6% for CONTROL, OBSERVATION, RESYNC 22, and RESYNC 30, respectively; P = 0.66), and no difference was observed for the second TAI (RESYNC 22 = 45.31% and RESYNC30 = 46.05%; P = 0.137), in the PR at the end of the breeding season (86.33%, 86.36%, 78.98%, and 81.52%, P = 0.43), or embryonic losses (4.54%, 2.85%, 6.45% and 7.40%, respectively; P = 0.61), but the percentage of crossbred pregnancy was higher in groups with resynchronisation (RESYNC22 and RESYNC30) than CONTROL and OBSERVATION (98.38%, 90.62%, 63.30%, 78.95%, P &lt; 0.001). In conclusion, resynchronisation programs of 22 or 30 days are more efficient to produce AI products, and the final pregnancy rate is similar among the treatments, differing only in the amount of calves produced by AI.

scholarly journals The Effect of A PRID®Delta-Based Estrus Synchronization Protocol On Pregnancy Rates In Cows With No Estrus Symptoms

2022 ◽  
Author(s):  
Murat Can Demir ◽  
Cihan Kaçar ◽  
Umut Çağın Arı ◽  
Semra Kaya ◽  
Oğuz Merhan ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Pregnancy Rates ◽  
Dominant Follicle ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Follicle Diameter ◽  
Follicle Size ◽  
Equine Chorionic Gonadotropin

Abstract The present study aimed to determine the effects of progesterone-based gonadotropin-releasing hormone (GnRH), prostaglandin F2α (PGF2α), and equine chorionic gonadotropin (eCG) injections on progesterone profiles and pregnancy rates in cows with no estrus symptoms within 60 days after parturition. A total of 80 cows were included in the study. All animals had the progesterone-releasing device PRID®Delta placed intravaginally for nine days with an injection of GnRH. On the eighth day, PGF2α was injected, and PRID®Delta was removed from the vagina on day nine. Artificial insemination was carried out 60 hours after PRID®Delta removal. In half of the animals (n = 40), 600 IU of eCG was injected when PRID®Delta was removed on the ninth day before artificial insemination 60 hours later. Blood samples were taken from the tail vein on days 0 and 8 to determine progesterone levels. The pregnancy rate in the group that received eCG was 37.5%, while it was 27.5% in those that did not (P = 0.4). While the dominant follicle diameter was 15.5 mm in cows injected with eCG during timed artificial insemination, the follicle diameter was 12.4 mm in cows with no eCG injection (P <0.001). There were no differences in serum progesterone values in blood samples taken until the time of artificial insemination. However, progesterone values in the blood taken during artificial insemination were 0.94 ng/ml in the eCG- group and 0.72 ng/ml in the eCG+ group (P <0.05). As a result, it was determined that eCG injections, in addition to progesterone-based GnRH and PGF2α applications, increased the pregnancy rates in cows without symptoms of estrus. The dominant follicle diameter was larger in cows treated with eCG during artificial insemination; however, follicle size did not increase the pregnancy rate.

PLASMA PROGESTERONE LEVELS IN CYCLING AND GONADOTROPHIN–PROSTAGLANDIN-TREATED HEIFERS

1976 ◽  
Vol 56 (1) ◽  
pp. 37-42 ◽  
Author(s):  
R. RAJAMAHENDRAN ◽  
P. C. LAGUË ◽  
R. D. BAKER
Keyword(s):  
Peak Level ◽  
Ovarian Activity ◽  
Corpora Lutea ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Dairy Heifers ◽  
The Mean

Progesterone levels were estimated by radioimmunoassay in blood samples obtained by venipuncture on the day of estrus and every alternate day until the onset of the next estrus in eight cycling dairy heifers. The mean level of progesterone was < 1 ng/ml during the first 2 days of the cycle, increased rapidly over the 4th–12th day period and reached a peak level value of 5.2 ± 1.1 ng/ml on day 14. Thereafter, the level declined rapidly to 2.6 ± 0.6 ng/ml on day 16 and then more gradually to 0.4 ± 0.1 ng/ml on day 21. In the second experiment, eight cycling heifers at diestrus were treated with gonadotrophin (2,000 IU PMSG or 1,000 IU PMSG + 1,000 IU HCG) followed 48 h later by 15 mg prostaglandin (PGF2α). Mid-ventral laparotomies were performed 4 days after the onset of estrus to observe ovarian activity. Progesterone levels were considerably higher in some animals and were slightly higher on the average after gonadotrophin treatments. The number of corpora lutea (CL) in these heifers ranged from 1 to 17. Progesterone levels of three heifers with 4–9 CL did not differ (P > 0.05) from those of three heifers with single CL. Two heifers each with 17 CL had peak progesterone levels of 38.4 and 27.8 ng/ml which were still high (9.6 and 26.5 ng/ml) by day 21. The remaining six heifers had low progesterone levels (< 1 ng/ml) by days 8–14, indicating premature regression of the CL. Thus, progesterone levels were not correlated with the number of CL.

2 EVIDENCE FOR THE PRESENCE OF OVULATION-INDUCING FACTOR IN PORCINE AND EQUINE SEMINAL PLASMA

2009 ◽  
Vol 21 (1) ◽  
pp. 101 ◽  
Author(s):  
O. A. Bogle ◽  
D. Ambati ◽  
R. P. Davis ◽  
G. P. Adams
Keyword(s):  
Seminal Plasma ◽  
Optimal Dose ◽  
Positive Control ◽  
Negative Control ◽  
Response To Treatment ◽  
Preovulatory Follicle ◽  
Progesterone Concentration ◽  
Control Groups ◽  
Plasma Progesterone ◽  
Blood Samples

The presence of an ovulation-inducing factor (OIF) in the seminal plasma of llamas and alpacas (reflex ovulators) and cattle (spontaneous ovulators) has been reported previously (Ratto MH et al. 2006 Theriogenology 66, 1102–1106). The presence of this protein in unrelated species supports the hypothesis that OIF is a conserved factor among species. The objectives of this study were to determine if OIF was present in equine and porcine seminal plasma, and whether the proportion of test animals (llamas) that ovulated in response to treatment with seminal plasma was related to dose. In Experiment 1, female llamas were assigned randomly to four groups (n = 8 or 9 per group) and treated intramuscularly with 1 mL llama seminal plasma (positive control), 3 mL equine seminal plasma, 3 mL porcine seminal plasma, or 2 mL saline (negative control). Ovulation and maximum corpus luteum diameter were compared using ultrasonography and confirmed with blood samples taken on Day 7 (Day 0 = day of treatment) to determine plasma progesterone concentration. The diameter of the preovulatory follicle at the time of treatment did not differ among groups. Equine seminal plasma induced ovulations in 3/8 (38%) llamas compared to 0/8 (0%) llamas treated with saline or porcine seminal plasma (P = 0.1). The proportion of females that ovulated was lower in the equine group (P < 0.01) compared with those animals treated with llama seminal plasma (9/9; 100%). Of the animals that ovulated, maximum CL diameter did not differ between llama and equine seminal plasma-treated groups (mean ± SEM; 11.1 ± 1.1, 11.5 ± 1.5, respectively). Similarly, progesterone concentrations were not different among llamas treated with llama seminal plasma or equine seminal plasma (mean ± SEM; 3.1 ± 0.4, 3.7 ± 1.2, respectively). The design of Experiment 2 was the same, but the dose of equine and porcine seminal plasma was increased to 8 mL and 10 mL, respectively. The proportion of females that ovulated was less (P < 0.05) in equine (2/9) and porcine (3/9) seminal plasma groups compared with the group treated with llama seminal plasma (9/9). There were no ovulations detected in llamas treated with saline (0/8). Although differences between equine, porcine, and negative control groups did not reach significance, results provide some evidence for the presence of OIF in equine and porcine seminal plasma. The effect of dose of equine and porcine seminal plasma is equivocal, suggesting that the concentration of OIF in the seminal plasma of these species may be very low and the optimal dose for inducing ovulation in test animals had not been reached. Research supported by the Natural Sciences and Engineering Council of Canada.

188 EFFECT OF RABBIT SEMINAL PLASMA IN OVULATING RESPONSE

2013 ◽  
Vol 25 (1) ◽  
pp. 243 ◽  
Author(s):  
M. Masdeu ◽  
R. M. García-García ◽  
P. Millán ◽  
L. Revuelta ◽  
O. G. Sakr ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Protein Concentration ◽  
Saline Solution ◽  
Experimental Period ◽  
Positive Control ◽  
Negative Control ◽  
Corpora Lutea ◽  
Blood Samples ◽  
Equine Chorionic Gonadotropin ◽  
Artificial Vagina

The presence of an ovulation-inducing factor (OIF) in the seminal plasma (SP) of several species with spontaneous and induced ovulation, included the rabbit, has been documented. The biochemical identity of OIF in SP remains unknown, but it seems that OIF is a protein (Ratto et al. 2011 Reprod. Biol. Endocrinol. 9, 24). The aim of this study was to determine if the protein present in the rabbit SP could induce ovulation in a dose manner and provoke changes in plasma hormone concentrations [LH and progesterone (P4)]. Semen was collected from 12 male rabbits using an artificial vagina, pooled, centrifugated at 3000g for 30 min twice and analysed by Bradford method to determine protein concentration that was 7 mg protein mL–1 of SP. After storage at –80°C, the SP was lyophilized for use at different concentrations. Twenty-four females were synchronized with an i.m. injection of 25 IU of equine chorionic gonadotropin and randomly assigned to 4 groups (n = 6). Forty-eight hours later (day 0) they were given a single i.m. dose of 1) 1 mL of saline solution (SS; negative control), 2) 20 µg of gonadorelin (GnRH; positive control), 3) 1 mL of lyophilized SP diluted in SS containing 7 mg of protein (SP7), 4) 1 mL of lyophilized SP diluted in SS containing 14 mg of protein (SP14). Blood samples for LH measurement were taken every 30 min from 30 min before injection to 2 h after treatment. Blood samples for P4 measurement were taken every 2 days from Day 0 to Day 6. Hormone determinations were made by enzyme immunoassay. Ovulation rate (OR), number of corpora lutea (CL), follicles higher than 1 mm, and total number of hemorrhagic follicles were determined after euthanasia on Day 7. Statistical analysis was performed by ANOVA. The OR was significantly higher (P < 0.0001) in GnRH than in SS, SP7, and SP14 groups (OR: 100, 0, 0, and 8.3%, respectively). Total number of CL counted in does that ovulated in GnRH and SP14 groups was not different (13.7 ± 0.8 and 9 ± 0.0 CL, respectively; P < 0.0001). No statistical differences were observed between groups on the number of follicles higher than 1 mm (GnRH: 17 ± 2.4; SS: 15 ± 1.6; SP7: 11.7 ± 2.6; SP14: 14.8 ± 0.9) and anovulatory hemorrhagic follicles (GnRH: 2.3 ± 0.9; SS: 0.2 ± 0.2; PS: 1.7 ± 0.8; PS 14: 1.7 ± 1.5). Treatment was followed by a surge in plasma LH concentration beginning 30 min after treatment to 120 min in GnRH group ranging ~75 ng mL–1, whereas in the other groups it remained at basal levels (around 20 ng mL–1; P < 0.0001). Plasma P4 concentrations were significantly increased from Day 2 to 6 (4.7 ± 0.7 to 22.3 ± 3.7 ng mL–1; P < 0.0001) only in rabbits treated with GnRH. Plasma P4 concentrations did not vary throughout the experimental period in all OIF-treated rabbits. The present study failed to demonstrate the effect of 3 different dosages of OIF of the rabbit SP on induction of ovulation. More studies are necessary to elucidate if rabbit SP could induce ovulation in rabbit females. We acknowledge CM and MEC for funding.

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