scholarly journals The Dual Role of the Potyvirus P3 Protein of Turnip mosaic virus as a Symptom and Avirulence Determinant in Brassicas

2003 ◽  
Vol 16 (9) ◽  
pp. 777-784 ◽  
Author(s):  
Carol E. Jenner ◽  
Xiaowu Wang ◽  
Kenta Tomimura ◽  
Kazusato Ohshima ◽  
Fernando Ponz ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Plant Virus ◽  
Turnip Mosaic Virus ◽  
Single Amino Acid ◽  
Infection Cycle ◽  
In Planta ◽  
Napus Line ◽  
The Uk

Two isolates of the potyvirus Turnip mosaic virus (TuMV), UK 1 and CDN 1, differ both in their general symptoms on the susceptible propagation host Brassica juncea and in their ability to infect B. napus lines possessing a variety of dominant resistance genes. The isolate CDN 1 produces a more extreme mosaic in infected brassica leaves than UK 1 and is able to overcome the resistance genes TuRB01, TuRB04, and TuRB05. The resistance gene TuRB03, in the B. napus line 22S, is effective against CDN 1 but not UK 1. The nucleic acid sequences of the UK 1 and CDN 1 isolates were 90% identical. The C-terminal half of the P3 protein was identified as being responsible for the differences in symptoms in B. juncea. A single amino acid in the P3 protein was found to be the avirulence determinant for TuRB03. Previous work already has identified the P3 as an avirulence determinant for TuRB04. Our results increase the understanding of the basis of plant-virus recognition, show the importance of the potyviral P3 gene as a symptom determinant, and provide a role in planta for the poorly understood P3 protein in a normal infection cycle.

scholarly journals Turnip mosaic virus determinants of virulence for Brassica napus resistance genes

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S155-S157
Author(s):  
C.E. Jenner ◽  
F. Sánchez ◽  
K. Tomimura ◽  
K. Ohshima ◽  
F. Ponz ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Infectious Clone ◽  
Turnip Mosaic Virus ◽  
Viral Fitness ◽  
Virulence Determinants ◽  
Similar Strategy ◽  
Napus Line

Dominant resistance genes identified in Brassica napus lines are effective against some, but not all, Turnip mosaic virus<br />(TuMV) isolates. An infectious clone of an isolate (UK 1) was used as the basis of chimeric virus constructions using<br />resistance-breaking mutants and other isolates to identify the virulence determinants for three dominant resistance genes.<br />For the resistance gene TuRB01, the presence of either of two mutations affecting the cylindrical inclusion (CI) protein<br />converted the avirulent UK 1 to a virulent isolate. Acquisition of such mutations had a slight cost to viral fitness in<br />plants lacking the resistance gene. A similar strategy is being used to identify the virulence determinants for two more<br />resistance genes present in another B. napus line.

scholarly journals Structure of Turnip mosaic virus and its viral-like particles

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Rebeca Cuesta ◽  
Carmen Yuste-Calvo ◽  
David Gil-Cartón ◽  
Flora Sánchez ◽  
Fernando Ponz ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Plant Virus ◽  
Plant Viruses ◽  
Turnip Mosaic Virus ◽  
Correct Orientation ◽  
Bona Fide ◽  
Rna Interaction ◽  
Genus One

Abstract Turnip mosaic virus (TuMV), a potyvirus, is a flexible filamentous plant virus that displays a helical arrangement of coat protein copies (CPs) bound to the ssRNA genome. TuMV is a bona fide representative of the Potyvirus genus, one of most abundant groups of plant viruses, which displays a very wide host range. We have studied by cryoEM the structure of TuMV virions and its viral-like particles (VLPs) to explore the role of the interactions between proteins and RNA in the assembly of the virions. The results show that the CP-RNA interaction is needed for the correct orientation of the CP N-terminal arm, a region that plays as a molecular staple between CP subunits in the fully assembled virion.

scholarly journals Molecular and Biological Characterisation of Turnip mosaic virus Isolates Infecting Poppy (Papaver somniferum and P. rhoeas) in Slovakia

Viruses ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 430 ◽  
Author(s):  
Miroslav Glasa ◽  
Katarína Šoltys ◽  
Lukáš Predajňa ◽  
Nina Sihelská ◽  
Slavomíra Nováková ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Turnip Mosaic Virus ◽  
Biological Assays ◽  
Genetic Complexity ◽  
The World ◽  
History Of ◽  
Virus Isolates

In recent years, the accumulated molecular data of Turnip mosaic virus (TuMV) isolates from various hosts originating from different parts of the world considerably helped to understand the genetic complexity and evolutionary history of the virus. In this work, four complete TuMV genomes (HC9, PK1, MS04, MS15) were characterised from naturally infected cultivated and wild-growing Papaver spp., hosts from which only very scarce data were available previously. Phylogenetic analyses showed the affiliation of Slovak Papaver isolates to the world-B and basal-B groups. The PK1 isolate showed a novel intra-lineage recombination pattern, further confirming the important role of recombination in the shaping of TuMV genetic diversity. Biological assays indicated that the intensity of symptoms in experimentally inoculated oilseed poppy are correlated to TuMV accumulation level in leaves. This is the first report of TuMV in poppy plants in Slovakia.

scholarly journals Patterns of recombination in turnip mosaic virus genomic sequences indicate hotspots of recombination

2007 ◽  
Vol 88 (1) ◽  
pp. 298-315 ◽  
Author(s):  
Kazusato Ohshima ◽  
Yasuhiro Tomitaka ◽  
Jeffery T. Wood ◽  
Yoshiteru Minematsu ◽  
Hiromi Kajiyama ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Gene Sequence ◽  
Turnip Mosaic Virus ◽  
Genomic Sequences ◽  
Composition Analysis ◽  
Sequence Composition ◽  
Field Samples ◽  
Exact Position ◽  
Complete Genomic

Potyviruses have variable single-stranded RNA genomes and many show clear evidence of recombination. This report studied the distribution of recombination sites in the genomes of 92 isolates of the potyvirus Turnip mosaic virus (TuMV); 42 came from the international gene sequence databases and an additional 50 complete genomic sequences were generated from field samples collected in Europe and Asia. The sequences were examined for evidence of recombination using seven different sequence comparison methods and the exact position of each site was confirmed by sequence composition analysis. Recombination sites were found throughout the genomes, except in the small 6K1 protein gene, and only 24 of the genomes (26 %) showed no evidence of recombination. Statistically significant clusters of recombination sites were found in the P1 gene and in the CI/6K2/VPg gene region. Most recombination sites were bordered by an upstream (5′) region of GC-rich and downstream (3′) region of AU-rich sequence of a similar length. Correlations between the presence and type of recombination site and provenance, host type and phylogenetic relationships are discussed, as is the role of recombination in TuMV evolution.

Natural isolates of Brome mosaic virus with the ability to move from cell to cell independently of coat protein

2005 ◽  
Vol 86 (4) ◽  
pp. 1201-1211 ◽  
Author(s):  
Atsushi Takeda ◽  
Wakako Nakamura ◽  
Nobumitsu Sasaki ◽  
Kaku Goto ◽  
Masanori Kaido ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Mutational Analysis ◽  
Cell Movement ◽  
Plant Viruses ◽  
Brome Mosaic Virus ◽  
Single Amino Acid ◽  
In Planta ◽  
C Terminus ◽  
Natural Isolates

Brome mosaic virus (BMV) requires encapsidation-competent coat protein (CP) for cell-to-cell movement and the 3a movement protein (MP) is involved in determining the CP requirement for BMV movement. However, these conclusions have been drawn by using BMV strain M1 (BMV-M1) and a related strain. Here, the ability of the MPs of five other natural BMV strains to mediate the movement of BMV-M1 in the absence of CP was tested. The MP of BMV M2 strain (BMV-M2) efficiently mediated the movement of CP-deficient BMV-M1 and the MPs of two other strains functioned similarly to some extent. Furthermore, BMV-M2 itself moved between cells independently of CP, demonstrating that BMV-M1 and -M2 use different movement modes. Reassortment between CP-deficient BMV-M1 and -M2 showed the involvement of RNA3 in determining the CP requirement for cell-to-cell movement and the involvement of RNAs 1 and 2 in movement efficiency and symptom induction in the absence of CP. Spontaneous BMV MP mutants generated in planta that exhibited CP-independent movement were also isolated and analysed. Comparison of the nucleotide differences of the MP genes of BMV-M1, the natural strains and mutants capable of CP-independent movement, together with further mutational analysis of BMV-M1 MP, revealed that single amino acid differences at the C terminus of MP are sufficient to alter the requirement for CP in the movement of BMV-M1. Based on these findings, a possible virus strategy in which a movement mode is selected in plant viruses to optimize viral infectivity in plants is discussed.

scholarly journals Resistance Phenotypes in Diverse Accessions, Breeding Lines, and Cultivars of Three Mustard Species Inoculated with Turnip mosaic virus

Plant Disease ◽  
2010 ◽  
Vol 94 (11) ◽  
pp. 1290-1298 ◽  
Author(s):  
Monica A. Kehoe ◽  
Brenda A. Coutts ◽  
Roger A. C. Jones
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Indian Mustard ◽  
Systemic Infection ◽  
Turnip Mosaic Virus ◽  
Biofuel Production ◽  
Systemic Necrosis ◽  
Breeding Lines ◽  
B Genome ◽  
A Genome

The responses of 44 accessions, breeding lines, or cultivars of Brassica juncea (Indian mustard), 9 of B. carinata (Ethiopian mustard), 5 of B. nigra (black mustard), and 6 crosses between B. juncea and B. napus (canola) to sap inoculation with Turnip mosaic virus (TuMV) were investigated. Eleven different phenotypes were obtained, including six previously recognized in B. napus (+, O, R, RN, RN/+, and +N) and five not recorded before (+St, RN/St, RN/St/+, +N1, and +ND). All but two (+ and +St) were resistance phenotypes. The resistance phenotypes in B. carinata and B. juncea × B. napus crosses prevented systemic infection but those in B. juncea and B. nigra included systemic necrosis. Absence of systemic invasion associated with resistance phenotypes in B. carinata was confirmed by graft inoculations. The resistance phenotypes may reflect the presence of known TuMV resistance genes located in the A genome or unknown genes in the B genome in B. juncea, unknown resistance genes in the B or C genomes in B. carinata, and unknown resistance genes in the B genome in B. nigra. Further research to identify the resistance genes involved would establish the potential usefulness of these resistance phenotypes in breeding TuMV-resistant mustard cultivars for biofuel production.

Role of a Newly Cloned Alternative Oxidase Gene (BjAOX1a) in Turnip Mosaic Virus (TuMV) Resistance in Mustard

2011 ◽  
Vol 30 (2) ◽  
pp. 309-318 ◽  
Author(s):  
Lei Zhu ◽  
Yanman Li ◽  
Neelam Ara ◽  
Jinghua Yang ◽  
Mingfang Zhang
Keyword(s):  
Mosaic Virus ◽  
Alternative Oxidase ◽  
Turnip Mosaic Virus ◽  
Oxidase Gene ◽  
Tumv Resistance

scholarly journals Visualization of the Interaction between the Precursors of VPg, the Viral Protein Linked to the Genome of Turnip Mosaic Virus, and the Translation Eukaryotic Initiation Factor iso 4E In Planta

2006 ◽  
Vol 81 (2) ◽  
pp. 775-782 ◽  
Author(s):  
Chantal Beauchemin ◽  
Nathalie Boutet ◽  
Jean-François Laliberté
Keyword(s):  
Mosaic Virus ◽  
Viral Protein ◽  
Fluorescent Protein ◽  
Turnip Mosaic Virus ◽  
Bimolecular Fluorescence Complementation ◽  
Initiation Factor ◽  
Eukaryotic Initiation Factor ◽  
In Planta ◽  
Translation Factor ◽  
Cytoplasmic Vesicles

ABSTRACT The RNA genome of Turnip mosaic virus is covalently linked at its 5′ end to a viral protein known as VPg. This protein binds to the translation eukaryotic initiation factor iso 4E [eIF(iso)4E]. This interaction has been shown to be important for virus infection, although its exact biological function(s) has not been elucidated. In this study, we investigated the subcellular site of the VPg-eIF(iso)4E interaction using bimolecular fluorescence complementation (BiFC). As a first step, eIF(iso)4E, 6K-VPg-Pro, and VPg-Pro were expressed as full-length green fluorescent protein (GFP) fusions in Nicotiana benthamiana, and their subcellular localizations were visualized by confocal microscopy. eIF(iso)4E was predominantly associated with the endoplasmic reticulum (ER), and VPg-Pro was observed in the nucleus and possibly the nucleolus, while 6K-VPg-Pro-GFP induced the formation of cytoplasmic vesicles budding from the ER. In BiFC experiments, reconstituted green fluorescence was observed throughout the nucleus, with a preferential accumulation in subnuclear structures when the GFP split fragments were fused to VPg-Pro and eIF(iso)4E. On the other hand, the interaction of 6K-VPg-Pro with eIF(iso)4E was observed in cytoplasmic vesicles embedded in the ER. These data suggest that the association of VPg with the translation factor might be needed for two different functions, depending of the VPg precursor involved in the interaction. VPg-Pro interaction with eIF(iso)4E may be involved in perturbing normal cellular functions, while 6K-VPg-Pro interaction with the translation factor may be needed for viral RNA translation and/or replication.

scholarly journals Role of recombination in the evolution of natural populations of Cucumber mosaic virus, a tripartite RNA plant virus

Virology ◽  
2005 ◽  
Vol 332 (1) ◽  
pp. 359-368 ◽  
Author(s):  
Julien Bonnet ◽  
Aurora Fraile ◽  
Soledad Sacristán ◽  
José M. Malpica ◽  
Fernando García-Arenal
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Virus ◽  
Natural Populations
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