The effects of copper and nitrogen supply on the retranslocation of copper in four cultivars of wheat

1978 ◽  
Vol 29 (5) ◽  
pp. 925 ◽  
Author(s):  
J Hill ◽  
AD Robson ◽  
JF Loneragan

The retranslocation of copper and nitrogen was studied in four cultivars of wheat grown in pots of a copper-deficient sand from Lancelin, W.A. Plants were grown at two levels of nitrogen and three levels of copper, and harvested three times during growth and at maturity. Plants grown at low copper were severely copper-deficient and yielded no grain. At low nitrogen, plants at marginal copper gave similar grain yields to plants at high copper. Application of high nitrogen at marginal copper either had little effect or depressed grain yield. Application of high nitrogen at high copper gave a strong positive interaction producing maximal grain yield in each cultivar. Low copper supply delayed yellowing and the decline in copper and nitrogen content of the oldest leaf of each cultivar. Application of high nitrogen further delayed yellowing of the oldest leaf and loss of its copper and nitrogen. Probably the copper content of the oldest leaf does not decline until the leaf begins to senesce. Senesced leaves retain low concentrations of copper and nitrogen. It is suggested that at marginal copper, copper retention by senesced vegetation limits the retranslocation of copper from vegetation to grain. Susceptibility to copper deficiency differed markedly with stage of growth and with cultivar. At marginal copper and high nitrogen, copper deficiency almost eliminated the grain yield of Argentine IX, but had relatively small effects on Gamenya, Olympic, and Petit Rojo. The higher susceptibility of grain production in Argentine IX to copper deficiency was not related to its copper content in whole tops or to its grain protein concentration. The susceptibility may have resulted from the ability of this cultivar to form large numbers of tillers which competed with the developing grain for retranslocated copper.

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1295
Author(s):  
Ahossi Patrice Koua ◽  
Mirza Majid Baig ◽  
Benedict Chijioke Oyiga ◽  
Jens Léon ◽  
Agim Ballvora

Nitrogen (N) is a vital component of crop production. Wheat yield varies significantly under different soil available N. Knowing how wheat responds to or interacts with N to produce grains is essential in the selection of N use efficient cultivars. We assessed in this study variations among wheat genotypes for productivity-related traits under three cropping systems (CS), high-nitrogen with fungicide (HN-WF), high-nitrogen without fungicide (HN-NF) and low-nitrogen without fungicide (LN-NF) in the 2015, 2016 and 2017 seasons. ANOVA results showed genotypes, CS, and their interactions significantly affected agronomic traits. Grain yield (GY) increased with higher leaf chlorophyll content, importantly under CS without N and fungicide supply. Yellow rust disease reduced the GY by 20% and 28% in 2015 and 2016, respectively. Moreover, averaged over growing seasons, GY was increased by 23.78% under CS with N supply, while it was greatly increased, by 52.84%, under CS with both N and fungicide application, indicating a synergistic effect of N and fungicide on GY. Fungicide supply greatly improved the crop ability to accumulate N during grain filling, and hence the grain protein content. Recently released cultivars outperformed the older ones in most agronomic traits including GY. Genotype performance and stability analysis for GY production showed differences in their stability levels under the three CS. The synergistic effect of nitrogen and fungicide on grain yield (GY) and the differences in yield stability levels of recently released wheat cultivars across three CS found in this study suggest that resource use efficiency can be improved via cultivar selection for targeted CS.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mathias Frontini ◽  
Arnaud Boisnard ◽  
Julien Frouin ◽  
Malika Ouikene ◽  
Jean Benoit Morel ◽  
...  

Abstract Background Nitrogen fertilization is known to increase disease susceptibility, a phenomenon called Nitrogen-Induced Susceptibility (NIS). In rice, this phenomenon has been observed in infections with the blast fungus Magnaporthe oryzae. A previous classical genetic study revealed a locus (NIS1) that enhances susceptibility to rice blast under high nitrogen fertilization. In order to further address the underlying genetics of plasticity in susceptibility to rice blast after fertilization, we analyzed NIS under greenhouse-controlled conditions in a panel of 139 temperate japonica rice strains. A genome-wide association analysis was conducted to identify loci potentially involved in NIS by comparing susceptibility loci identified under high and low nitrogen conditions, an approach allowing for the identification of loci validated across different nitrogen environments. We also used a novel NIS Index to identify loci potentially contributing to plasticity in susceptibility under different nitrogen fertilization regimes. Results A global NIS effect was observed in the population, with the density of lesions increasing by 8%, on average, under high nitrogen fertilization. Three new QTL, other than NIS1, were identified. A rare allele of the RRobN1 locus on chromosome 6 provides robust resistance in high and low nitrogen environments. A frequent allele of the NIS2 locus, on chromosome 5, exacerbates blast susceptibility under the high nitrogen condition. Finally, an allele of NIS3, on chromosome 10, buffers the increase of susceptibility arising from nitrogen fertilization but increases global levels of susceptibility. This allele is almost fixed in temperate japonicas, as a probable consequence of genetic hitchhiking with a locus involved in cold stress adaptation. Conclusions Our results extend to an entire rice subspecies the initial finding that nitrogen increases rice blast susceptibility. We demonstrate the usefulness of estimating plasticity for the identification of novel loci involved in the response of rice to the blast fungus under different nitrogen regimes.


Analyses of the alimentary contents flowing to the duodenum of sheep during 24 h show that when the sheep are consuming a low-nitrogen diet more total nitrogen and amino nitrogen pass to the duodenum than are eaten daily in the food whereas when the sheep are eating high nitrogen diets, less total nitrogen and less amino nitrogen pass to the duodenum. The disparity between the total nitrogen and amino nitrogen content of the diets largely disappeared by the time the alimentary contents reached the terminal part of the ileum. From 64 to 68% of the nitrogen entering the duodenum and 54 to 64% of the nitrogen in the ileal contents was in the form of amino nitrogen. Proportionately more of the amino nitrogen was in solution in the ileal contents than in the duodenal contents. Losses of amino acids in the stomach when a high-nitrogen diet was consumed were especially large for glutamic acid, aspartic acid, proline, arginine and leucine. They were least for cystine and threonine. Gains of amino acids in the stomach when low nitrogen diets were consumed were all substantial except for proline, where a loss was found when hay and flaked maize were given. When these changes are considered as proportions of the quantities eaten then trends are similar for all acids. Changes in the molar proportions of the amino acids present in hydrolysates of the duodenal and ileal contents are discussed together with the significance of these changes in relation to the nutrition of the sheep.


2018 ◽  
Vol 29 (6) ◽  
pp. 1323-1330 ◽  
Author(s):  
Xiran Wang ◽  
Shizhong Wei ◽  
Liujie Xu ◽  
Jiwen Li ◽  
Xiuqing Li ◽  
...  

1998 ◽  
Vol 26 (5) ◽  
pp. 628-635 ◽  
Author(s):  
Anna Giovanetti ◽  
Luisa Rossi ◽  
Mariateresa Mancuso ◽  
Carmine C. Lombardi ◽  
Maria Rita Marasco ◽  
...  

2020 ◽  
Vol 21 (2) ◽  
pp. 543 ◽  
Author(s):  
Berhanu Tadesse Ertiro ◽  
Michael Olsen ◽  
Biswanath Das ◽  
Manje Gowda ◽  
Maryke Labuschagne

Understanding the genetic basis of maize grain yield and other traits under low-nitrogen (N) stressed environments could improve selection efficiency. In this study, five doubled haploid (DH) populations were evaluated under optimum and N-stressed conditions, during the main rainy season and off-season in Kenya and Rwanda, from 2014 to 2015. Identifying the genomic regions associated with grain yield (GY), anthesis date (AD), anthesis-silking interval (ASI), plant height (PH), ear height (EH), ear position (EPO), and leaf senescence (SEN) under optimum and N-stressed environments could facilitate the use of marker-assisted selection to develop N-use-efficient maize varieties. DH lines were genotyped with genotyping by sequencing. A total of 13, 43, 13, 25, 30, 21, and 10 QTL were identified for GY, AD ASI, PH, EH, EPO, and SEN, respectively. For GY, PH, EH, and SEN, the highest number of QTL was found under low-N environments. No common QTL between optimum and low-N stressed conditions were identified for GY and ASI. For secondary traits, there were some common QTL for optimum and low-N conditions. Most QTL conferring tolerance to N stress was on a different chromosome position under optimum conditions.


2019 ◽  
Vol 20 (23) ◽  
pp. 5893 ◽  
Author(s):  
Wei Xin ◽  
Lina Zhang ◽  
Wenzhong Zhang ◽  
Jiping Gao ◽  
Jun Yi ◽  
...  

Nitrogen is an essential nutrient for plant growth and basic metabolic processes. Root systems play an important role in the ability of plants to obtain nutrients from the soil, and are closely related to the growth and development of above-ground plants. Root morphology analysis showed that root growth was induced under low-nitrogen conditions and inhibited under high-nitrogen conditions. To better understand the molecular mechanisms and metabolic basis underlying the rice root response to nitrogen availability, an integrated analysis of the rice root transcriptome and metabolome under three environmental conditions (low-, control, and high-nitrogen conditions) was conducted. A total of 262 and 262 differentially level metabolites were identified under low- and high-nitrogen conditions, respectively. A total of 696 and 808 differentially expressed genes were identified under low- and high-nitrogen conditions, respectively. For both the differentially expressed genes and metabolites, KEGG pathway analysis indicated that amino acid metabolism, carbon and nitrogen metabolism, phenylpropanoid metabolism, and phytohormones’ signal transduction were significantly affected by nitrogen availability. Additionally, variable levels of 65 transcription factors (TFs) were identified in rice leaves exposed to high and low nitrogen, covering 22 TF families. These results also indicate that there is a significant difference in the transcriptional regulation mechanisms of rice roots between low and high nitrogen. In summary, our study provides new information for a further understanding of the response of rice roots to low-nitrogen and high-nitrogen conditions.


1980 ◽  
Vol 20 (107) ◽  
pp. 717 ◽  
Author(s):  
NJ Grundon

One field and two glasshouse trials were conducted to test the effectiveness of soil and foliar applications of copper sulphate in correcting copper deficiency of wheat on a severely deficient, nearneutral clay soil in the Western Downs region of Queensland. In the field, when wheat was stressed for water from late tillering (Feekes stage 5) to anthesis (Feekes stage 11) , soil dressings of 2.5-10.0 kg CuSO4.5H2O ha-1 increased early vegetative growth but foliar symptoms of copper deficiency reappeared during stem elongation, and grain yields were negligible at all rates. A single foliar spray of 2% CuSO4.5H2O applied at mid-tillering (Feekes stage 3) also did not correct the deficiency completely: foliar symptoms reappeared and grain yield was low (141 kg ha-1). A double spray treatment, the first applied at mid-tillering and the second just before booting (Feekes stage 10), was more effective and resulted in a grain yield of 800 kg ha-1. In a glasshouse experiment supplied with adequate water, a soil dressing equivalent to 16 kg CuSO4.5H2O ha-1 (3.2 mg Culpot) completely corrected the deficiency. In a second glasshouse experiment, 2% CuSO4.5H2O solution was applied as single sprays at Feekes stages 3, 8, 9, 10 or 11, or as double sprays at Feekes stages 3 + 8, 3 + 9, 3 + 10 or 3 + 11. The most effective single spray was that applied at Feekes stage 10, but maximum dry matter and grain yield were obtained when a double spray was applied at Feekes stage 3 + 10. The effectiveness of soil and foliar applications of CuSO4.5H2O for correcting Cu deficiency of dryland wheat in Queensland soils are discussed in relation to water regime and supplying adequate Cu for both vegetative and reproductive growth.


1990 ◽  
Vol 30 (5) ◽  
pp. 687 ◽  
Author(s):  
RF Brennan

The effectiveness of copper oxychloride (CU2Cl(OH)3, 52% Cu) and chelated Cu (Cu-EDTA, 15% Cu) were compared with the effectiveness of copper sulphate (CuSO4, 25% Cu) as foliar sprays for alleviating Cu deficiency and obtaining maximum grain yields of wheat (1.93-2.5 t/ha). The experiments were conducted over 4 years at 4 sites in the Lake Grace and Newdegate districts, about 300-350 km south-east of Perth, Western Australia. Each source was sprayed at 6 or 7 rates of Cu to define the relationship between grain yield and the amount of foliar Cu applied for wheat grown on soils where Cu had not been previously applied. The levels of Cu sprayed in experiment 1 were 0, 21, 63, 125, 250, and 375 g/ha, and for experiments 2,3 and 4, the levels of Cu were 0, 25, 50, 100, 200, 400 and 800 g/ha. The relative effectiveness of foliar-applied chelated Cu and CU2Cl(OH)3, compared with CuSO4, was 1.72-2.24 and 0.47-0.63, respectively. Although the relative effectiveness of each product was different, similar quantities of each were required to achieve maximum wheat grain yield because of the difference in the Cu contents of each source of Cu. The amounts of Cu product sprayed for maximum grain yields of wheat varied within the ranges 0.9-1.8 kg/ha, 0.8-1.2 kg/ha and 0.8-1.8 kg/ha for CuSO4, chelated Cu and CU2Cl(OH)3, respectively.


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