Studies in uterotrophic activities in strains of subterranean clover

1989 ◽  
Vol 40 (4) ◽  
pp. 871 ◽  
Author(s):  
HL Davies
Keyword(s):  
Subterranean Clover ◽  
Biochanin A ◽  
Uterine Weight ◽  
The Mean ◽  
Negative Controls

Uterotrophic responses in ovariectomized ewes when fed or grazed on Mount Barker, Yarloop or Dwalganup varieties of subterranean clover are reported. The high formononetin varieties Dwalganup and Yarloop always elicited a uterotrophic response. The low-formononetin, high-genistein and biochanin A variety, Mount Barker, stimulated a uterotrophic response, using a 5-day bioassay, when fed at 200g/day or 800g/day, but little or no response when grazed. When chopped lucerne hay was offered with 200 g of the Mount Barker the uterotrophic response was suppressed and the mean uterine weight was not significantly different from that of the negative controls (P= 0.57). The uterine weight of sheep eating 200 g/day of Yarloop and offered lucerne chaff or pellets in one experiment was lower (but not significantly lower) than the unsupplemented group. This trend may be worthy of further investigation.

Adaptation by sheep to isoflavones in clover

1969 ◽  
Vol 9 (41) ◽  
pp. 569 ◽  
Author(s):  
FHW Morley ◽  
D Bennett ◽  
A Axelsen
Keyword(s):  
Subterranean Clover ◽  
Biochanin A ◽  
Uterine Weight ◽  
Pre Treatment ◽  
High Concentrations

Vaginal mucus scores and uterine weights of ovariectomized ewes that grazed subterranean clover for 13 days prior to tests were similar to those from ewes fed non-oestrogenic rations during this pre-treatment, if the leaves of test clovers contained high concentrations of formononetin (F) (Yarloop and Dinninup). No responses were obtained from subterranean clovers low in F, but high in genistein (G) or biochanin A (BA) (Clare and Bacchus Marsh) except for a group which was fed a non-oestrogenic diet during pre-treatment, and which gave a positive mucus test, but not a stgnificant uterine weight response. These results indicate that ewes can become non-responsive to G and BA, but not to F, and offer an explanation for some inconsistencies among bioassays of subterranean clovers. The task of selection and breeding of subterranean clover seems to be simplified since G and BA are unlikely to be harmful.

Quantitative aspects of phytooestrogen metabolism in sheep fed on subterranean clover (Trifolium subterraneum cultivar clare) or red clover (Trifolium pratense)

1970 ◽  
Vol 21 (5) ◽  
pp. 713 ◽  
Author(s):  
DA Shutt ◽  
RH Weston ◽  
JP Hogan
Keyword(s):  
Residence Time ◽  
Trifolium Pratense ◽  
Daily Intake ◽  
Red Clover ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Biochanin A ◽  
Free Form ◽  
The Mean ◽  
High Level

Studies have been made of the digestion and metabolism in Merino wethers of the isoflavones in subterranean clover (Trtfolium subterraneurn cv. Clare) and red clover (T. pratense). The dietary intake of isoflavones with both clovers was about 9 g per day. With the subterranean clover, the isoflavones were predominantly genistein and biochanin A, and slight teat length increases in the wethers ingesting this clover indicated a low level of oestrogenicity. With the red clover formononetin represented 60% of the isoflavone present and the wethers on this diet exhibited maximal teat length increases indicating a high level of oestrogenicity. Less than 1 % of the daily intake of the isoflavones was excreted as such in the faeces and urine; hence most of these compounds were metabolized or retained in the sheep. The dietary isoflavones were found to disappear rapidly from the rumen, and it was estimated by using marker techniques that the removal of these compounds from the stomach (rumen, reticulum, omasum, and abomasum) was virtually complete. Equol (7,4'-dihydroxyisoflavan), a metabolite of formononetin, was the predominant phyto-oestrogen in the digesta and excreta when red clover was given. The excretion of 3.9 g/day of this compound, mainly in urine, was equivalent to 70% of the intake of formononetin. It was calculated that about 86% of the equol produced in the rumen was absorbed from that organ; the mean residence time for equol in the rumen was estimated to be 1.7 hr. The isoflavones were present in blood plasma mainly in conjugated forms. Equol predominated with both clovers. The levels of equol were much lower with the subterranean clover than with the red clover diet; the concentrations of the conjugated form were respectively 13 and 300-440 �g/100 ml. Equol in the free form, although not detectable with the subterranean clover, was present at 4-10 �g/100 ml with red clover. The data were considered to be consistent with the conclusion that equol accounts for most of the phyto-oestrogenic activity in sheep fed on clovers containing high levels of formononetin.

Free and conjugated isoflavones in the plasma of sheep following ingestion of oestrogenic clover

1967 ◽  
Vol 18 (4) ◽  
pp. 647 ◽  
Author(s):  
DA Shutt ◽  
A Axelsen ◽  
HR Lindner
Keyword(s):  
Plasma Level ◽  
Total Concentration ◽  
Red Clover ◽  
Subterranean Clover ◽  
Inhibitory Effect ◽  
Water Soluble ◽  
Biochanin A ◽  
Uterine Weight ◽  
Sheep Grazing

The plasma of sheep grazing various species of oestrogenic clover contained water-soluble conjugates of daidzein, formononetin, genistein, and biochanin A, chiefly in the form of glucuronides, in concentrations up to 40 µg/100 ml. In sheep pen-fed on red clover or subterranean clover (cv. Dinninup and Yarloop) the total concentration of these four isoflavones 3–5 hr after feeding ranged from 150 to 720 µg/100 ml plasma, of which 1–2% only was present in "free" (i.e. unconjugated) form; by 24 hr the plasma level had fallen to < 5 µg/100 ml plasma. Preconditioning the pen-fed sheep to oestrogenic clover, or adding lucerne to the diet, had no significant effect on the concentration of "free" or conjugated isoflavones in the plasma and had no inhibitory effect on the uterine weight response. Comparison of the composition of circulating isoflavones to that of the clover ingested indicated partial demethylation of formononetin to daidzein and of biochanin A to genistein, and suggested that genistein and biochanin A were degraded more rapidly than formononetin or daidzein in the sheep. Equol, a metabolite previously isolated from the urine of sheep treated with formononetin, was present in the plasma at a concentration of 50–170 µg/100 ml 24 hr after feeding.

The assay of oestrogenic activity of Trifolium subterraneum L. by increase in uterine weight in the spayed guinea pig. II. Assays

1951 ◽  
Vol 2 (4) ◽  
pp. 480 ◽  
Author(s):  
G Alexander ◽  
RH Watson
Keyword(s):  
Guinea Pig ◽  
High Activity ◽  
Guinea Pigs ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Uterine Weight ◽  
Absolute Level ◽  
The Mean ◽  
The Absolute

When fed to spayed guinea pigs under the conditions outlined in Part I (Alexander and Watson 1951), whole clover from 83 per cent. of all samples and lamina from all of the few samples of this fraction yielded values of the mean log uterine weight in decigrams (mean log u.dg.) above 0.75, and provided data from which relative oestrogenic activities could be calculated by application of the previously derived statistics. The activity of fresh samples of four varieties of whole clover rose to a maximum in the mid-winter months, then decreased as the season progressed. However, both the absolute level and the magnitude and time relations of the changes varied with the different materials. Samples of lamina possessed high activity, while samples of petiole and stem exhibited little or no activity. Both the lamina and whole clover fractions of samples from grazed areas exhibited higher activity than corresponding material from adjacent ungrazed areas. Dehydration of clover reduced its activity. Little further change in activity occurred during storage of dried material over a period of 17 months. No demonstrable activity was present in the few species of plants other than subterranean clover which were tested.

Varietal reactions of Trifolium subterraneum L. to Phaseolus virus 2 Pierce.

1954 ◽  
Vol 5 (4) ◽  
pp. 598 ◽  
Author(s):  
EM Hutton ◽  
JW Peak
Keyword(s):  
Trypan Blue ◽  
Continuous Distribution ◽  
Systemic Infection ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Epidermal Cells ◽  
Severe Strain ◽  
Resistant Varieties ◽  
Lethal Reaction ◽  
The Mean

A number of varieties of subterranean clover (Trifolium subterraneum L.) were tested with a severe strain of Phaseolus virus 2 Pierce. Northam First Early, Dwalganup, and Pink Flowered gave lethal necrotic reactions while the rest developed mottles and chlorosis of different degrees of intensity. Reductions in the mean fresh weights of plants of five subterranean clover varieties ranged from 26.1 to 76.7 per cent. Epidermal cells of mottled leaves contained irregular aggregates of viroplasts stained by treatment with phloxine-trypan blue. Viroplasts in young tip leaves did not stain if the plants were held at temperatures below 44.6°F while those in mature basal leaves were not so affected. The lethal-reactors Northam First Early, Dwalganup, and Pink Flowered were resistant in the field to Phaseolus virus 2. With hand inoculation in the glass-house an average of 10 per cent. of plants of these varieties remained free from systemic infection, whereas all the plants of mottle-reacting varieties became systemically infected. The virus had a discontinous distribution in plants of lethal-reactors, compared with a continuous distribution in those of mottle-reactors such as Mount Barker. The lethal reaction is heritable in a dominant fashion in most crosses, so that the development of desirable new virus- resistant varieties is possible.

scholarly journals Assessment of Insecticidal Potency of Eggshell Powder Against Maize Weevil

2021 ◽  
Vol 4 (3) ◽  
pp. 41-50
Author(s):  
Olagunju O.D. ◽  
Rahman M.O. ◽  
Okparavero N.F. ◽  
Abah I.A. ◽  
Odutola B.S. ◽  
...  
Keyword(s):  
Weight Loss ◽  
Total Coliform ◽  
Positive Control ◽  
Ambient Condition ◽  
Maize Weevil ◽  
Synthetic Chemicals ◽  
Grain Damage ◽  
Chicken Eggshell ◽  
The Mean ◽  
Negative Controls

Efficacy of chicken eggshell powder at concentrations 0, 0.25, 0.5, 1.0 and 2.0 g /100 g maize, for control of Sitophilus zeamais, were compared with permethrin at dosage rate of 0.167g / 100 g of yellow maize. Experiments were conducted at Biotechnology laboratory (NSPRI), Ilorin under ambient condition (26±3oC 65±5% RH). Mortality of S. zeamais increased with increasing dosages of the CESP as well as with days of exposure. CESP at 2.0g gives 96.67% mortality which is not significantly different from the positive control. The mean progeny emergence (0.00%), weight loss (1.11%), and percentage grain damage (19.68%) by S. zeamais was significantly (P ≤ 0.05) decreased with increased dosage of CESP. However, CESP did not significantly affect seed germination. Total coliform count at 2.0 g (2.48 x 102) was significantly different from both positive and negative controls at p<0.05 Therefore, CESP can serve as a good maize protectant which can be used as substitute for synthetic chemicals.

scholarly journals Increasing adolescent firearm homicides and racial disparities following Florida’s ‘Stand Your Ground’ self-defence law

2019 ◽  
Vol 26 (2) ◽  
pp. 187-190 ◽  
Author(s):  
Michelle Degli Esposti ◽  
Douglas J Wiebe ◽  
Jason Gravel ◽  
David K Humphreys
Keyword(s):  
Racial Disparities ◽  
Black Adolescents ◽  
Interrupted Time Series ◽  
Time Varying ◽  
Series Design ◽  
Firearm Suicide ◽  
The Mean ◽  
The Law ◽  
Negative Controls ◽  
Interrupted Time Series Design

Establishing whether specific laws impact rates of firearm homicide in adolescents is critical for identifying opportunities to reduce preventable adolescent death. We evaluated Florida’s Stand Your Ground law, enacted October 2005, using an interrupted time series design from 1999 to 2017. We used segmented quasi-Poisson regression to model underlying trends in quarterly rates of adolescent (15–19 years) firearm homicide in Florida and disaggregated by race (Black/White). We used synthetic and negative controls (firearm suicide) to address time-varying confounding. Before Florida’s Stand Your Ground law, the mean quarterly rate was 1.53 firearm homicides per 100 000 adolescents. Black adolescents comprised 63.5% of all adolescent firearm homicides before and 71.8% after the law. After adjusting for trends, the law was associated with a 44.6% increase in adolescent firearm homicide. Our analysis indicates that Florida’s Stand Your Ground is associated with a significant increase in firearm homicide and may also exacerbate racial disparities.

Physiological and ecological studies on the oestrogenic isoflavones in subterranean clover (T. subterraneum L.) VII. Effects of nitrogen supply

1969 ◽  
Vol 20 (6) ◽  
pp. 1043 ◽  
Author(s):  
RC Rossiter
Keyword(s):  
Protein Synthesis ◽  
Nitrogen Deficiency ◽  
Subterranean Clover ◽  
Nitrogen Supply ◽  
The Other ◽  
Biochanin A ◽  
Ecological Studies ◽  
Carbon Substrates ◽  
Low Nitrogen ◽  
Practical Implications

In experiments with young plants of the Dwalganup strain of subterranean clover, nitrogen deficiency was associated with increased concentrations of isoflavones in the expanded leaves and cotyledons. In the first trifoliate leaves the concentration of total isoflavones (formononetin+genistein+ biochanin A) was approximately doubled at low nitrogen supply. Biochanin A was much less affected than the other two isoflavones. The increase in isoflavone contents of nitrogen-deficient leaves was associated with decreased protein synthesis, but not always with increased sugar contents. The supply of carbon substrates for isoflavone synthesis may depend on starch as well as sugar contents. Practical implications of the results are considered briefly.

The long-term influence of superphosphate and stocking rate on the production of spring-lambing Merino sheep in the high rainfall zone of southern Australia

1999 ◽  
Vol 50 (7) ◽  
pp. 1179 ◽  
Author(s):  
J. W. D. Cayley ◽  
G. A. Kearney ◽  
G. R. Saul ◽  
C. L. Lescun
Keyword(s):  
Fibre Diameter ◽  
Subterranean Clover ◽  
The Body ◽  
Stocking Rate ◽  
Gross Margin ◽  
Single Superphosphate ◽  
Merino Sheep ◽  
Predetermined Level ◽  
The Mean ◽  
Fleece Weight

The productivity of spring-lambing fine wool Merino sheep grazing pastures sown in 1977 to perennial ryegrass and subterranean clover was assessed from 1989 to 1998. The pastures were fertilised each autumn with single superphosphate at 6 levels, and were stocked at a low, medium, or high stocking rate (SR) at each level of fertiliser. The average phosphorus (P) applied annually since sowing (P ) ranged from 1.6 to 32.9 kg/ha. The SRs used varied with fertiliser level in that they were higher where more fertiliser had been applied, so that the highest SR at each level of fertiliser ensured that the pastures were well utilised. Each ewe raised 1 lamb, which was removed at weaning. The influence of fertiliser on the productivity of the sheep at 4 classes (1–4) of SR (mean SR = 7.1, 10.1, 12.6, and 18.2 ewes/ha for classes 1–4, respectively) was described by: y = A−BCP, where y represents production per sheep (kg), and A, B, and C are constants. For greasy fleece weight, estimates of B and C were 1.59 and 0.84; and for SR classes 1–4, the estimates of A were 5.06, 4.89, 4.78, and 4.46, respectively. For weaning weight of lambs, estimates of B and C were 8.4 and 0.82, and estimates of A were 23.5, 22.7, 21.5, and 20.9 for SR classes 1–4. The mean fibre diameter (µm) of the wool was described by: D = 14.18+1.48 GW, where GW is the mean greasy wool produced annually per sheep (kg) averaged over all sheep and years for each of the 18 treatments. The price (cents/kg) of wool with a fibre diameter D (P D) was given by: P D = 12197+4.94P2 + 688D−0.1945P20D − 5810√D, where 20 µm wool is P20 cents/kg. Supplements were fed if the body condition of ewes fell to a predetermined level. The supplement fed per ewe each year (S), expressed as metabolisable energy (in MJ) was described by: S = −602 − 44.1S R + 178.5P + 8.71S R P +539 √SR− 338.5√P−70.8P√SR, where S R and P represent the mean stocking rate (ewes/ha) and mean P applied annually. When a current set of costs and prices was applied to these equations, the maximum gross margin for a SR of 7.1 ewes/ha was $AU119/ha with 8.6 kg P/ha applied annually, and $AU262/ha for SR of 18.2 ewes/ha with 17.6 kg P/ha applied annually. If income derived from sheep is maintained constant, intensifying the sheep enterprise from the low to the high SR system would involve increasing sheep numbers by about 17%, but would release about 55% of the farm’s area for another purpose.

scholarly journals Analysis of Autoantibodies to 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase Using Different Technologies

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Lucile Musset ◽  
Makoto Miyara ◽  
Olivier Benveniste ◽  
Jean-Luc Charuel ◽  
Alexander Shikhman ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Coenzyme A ◽  
Clinical Phenotype ◽  
High Specificity ◽  
Necrotizing Myopathy ◽  
Nuclear Antigens ◽  
Autoantibody Profile ◽  
The Mean ◽  
Negative Controls ◽  
Statin Use

Diagnostic tests are needed to aid in the diagnosis of necrotizing myopathies associated with statin use. This study aimed to compare different technologies for the detection of anti-HMGCR antibodies and analyze the clinical phenotype and autoantibody profile of the patients. Twenty samples from myositis patients positive for anti-HMGCR antibodies using a research addressable laser bead assay and 20 negative controls were tested for autoantibodies to HMGCR: QUANTA Lite HMGCR ELISA and QUANTA Flash HMGCR CIA. All patients were also tested for antibodies to extractable nuclear antigens and myositis related antibodies. To verify the specificity of the ELISA, 824 controls were tested. All three assays showed qualitative agreements of 100% and levels of anti-HMGCR antibodies showed significant correlation: Spearman’s rho > 0.8. The mean age of the anti-HMGCR antibody positive patients was 54.4 years, 16/20 were females, and 18/20 had necrotizing myopathy (two patients were not diagnosed). Nine out of 20 anti-HMGCR positive patients were on statin. All patients with anti-HMGCR antibodies were negative for all other autoantibodies tested. Testing various controls showed high specificity (99.3%). Anti-HMGCR antibodies are not always associated with the use of statin and appear to be the exclusive autoantibody specificity in patients with statin associated myopathies.

Sign in / Sign up

Export Citation Format

Share Document