Hydrophilic Magnetite Nanoparticles Enhance Anticancer Activity of Anthracyclines In Vitro

2016 ◽  
Vol 69 (11) ◽  
pp. 1247
Author(s):  
Bin Yang ◽  
Lin Luo ◽  
Ying Ma ◽  
Chunyan Chen ◽  
Xiaoming Chen ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Light Scattering ◽  
Citric Acid ◽  
Anticancer Activity ◽  
Magnetite Nanoparticles ◽  
Resonance Light Scattering ◽  
Novel Method ◽  
Dna Chain ◽  
Nano Fe3o4

A novel method for enhancing the anticancer activity of anthracyclines in vitro was proposed by using hydrophilic magnetic nanoparticles. Citric acid-coated magnetite nanoparticles Fe3O4 (nano-Fe3O4-CA) interacted with anthracyclines by electrostatic and hydrophobic forces, resulting in the formation of aggregates (nano-Fe3O4-CA-drug). The aggregate was studied by resonance light scattering and fluorescence spectroscopy. The results indicated that in comparison with anthracyclines, the nano-Fe3O4-CA-drug showed high activity towards incorporation in the DNA chain. Furthermore, the cytotoxicity of nano-Fe3O4-CA-drug was investigated by cytotoxicity experiment and cell morphology study. The results confirmed that the nano-Fe3O4-CA-drug could inhibit the growth of cells more effectively than the drug alone. In conclusion, usage of nano-Fe3O4-CA affords reduction of the dosage of anthracyclines in vitro.

scholarly journals Magnetite Nanoparticles Coated with PEG 3350-Tween 80: In Vitro Characterization Using Primary Cell Cultures

Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 300 ◽  
Author(s):  
Jorge A Roacho-Pérez ◽  
Fernando G Ruiz-Hernandez ◽  
Christian Chapa-Gonzalez ◽  
Herminia G Martínez-Rodríguez ◽  
Israel A Flores-Urquizo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Magnetic Nanoparticles ◽  
Magnetite Nanoparticles ◽  
Spherical Shape ◽  
Tween 80 ◽  
Hemolysis Assay ◽  
Adipose Mesenchymal Stem Cells ◽  
Average Size

Some medical applications of magnetic nanoparticles require direct contact with healthy tissues and blood. If nanoparticles are not designed properly, they can cause several problems, such as cytotoxicity or hemolysis. A strategy for improvement the biological proprieties of magnetic nanoparticles is their functionalization with biocompatible polymers and nonionic surfactants. In this study we compared bare magnetite nanoparticles against magnetite nanoparticles coated with a combination of polyethylene glycol 3350 (PEG 3350) and polysorbate 80 (Tween 80). Physical characteristics of nanoparticles were evaluated. A primary culture of sheep adipose mesenchymal stem cells was developed to measure nanoparticle cytotoxicity. A sample of erythrocytes from a healthy donor was used for the hemolysis assay. Results showed the successful obtention of magnetite nanoparticles coated with PEG 3350-Tween 80, with a spherical shape, average size of 119.2 nm and a zeta potential of +5.61 mV. Interaction with mesenchymal stem cells showed a non-cytotoxic propriety at doses lower than 1000 µg/mL. Interaction with erythrocytes showed a non-hemolytic propriety at doses lower than 100 µg/mL. In vitro information obtained from this work concludes that the use of magnetite nanoparticles coated with PEG 3350-Tween 80 is safe for a biological system at low doses.

Preparation, characterization and in vitro anticancer activity of paclitaxel conjugated magnetic nanoparticles

2018 ◽  
Vol 44 (11) ◽  
pp. 1895-1903 ◽  
Author(s):  
Mahsa Tarantash ◽  
Hamed Nosrati ◽  
Hamidreza Kheiri Manjili ◽  
Ali Baradar Khoshfetrat
Keyword(s):  
Magnetic Nanoparticles ◽  
Anticancer Activity

scholarly journals The synthesis, characterization and applications of poly[N-isopropylacrylamide-co-3-allyloxy-1,2-propanediol] grafted onto modified magnetic nanoparticles

RSC Advances ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 3511-3519
Author(s):  
Arash Alipour ◽  
Mehrnaz Babaei Shekardasht ◽  
Parvin Gharbani
Keyword(s):  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Drug Delivery Systems ◽  
Biological Fluids ◽  
Delivery Systems ◽  
Novel Method ◽  
Modified Magnetic Nanoparticles

In this paper, a novel method is investigated for the extraction, determination, and delivery of ceftazidime in simulated gastric and real biological fluids such as serum plasma and urine in in vitro drug delivery systems.

Preparation of low-molecular-weight chitosan derivative zinc complexes and their effect on the growth of liver cancer cells in vitro

2009 ◽  
Vol 81 (12) ◽  
pp. 2397-2405 ◽  
Author(s):  
Rong-Min Wang ◽  
Nai-Pu He ◽  
Peng-Fei Song ◽  
Yu-Feng He ◽  
Lan Ding ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Schiff Base ◽  
Light Scattering ◽  
Liver Cancer ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Electrostatic Interactions ◽  
Low Molecular Weight ◽  
Liver Cancer Cells

Low-molecular-weight (LMW) chitosan salicylaldehyde Schiff-base and its zinc(II) complexes were synthesized and characterized by Fourier transform-infrared (FT-IR) spectra, transmission electron microscopy (TEM), dynamic light scattering (DLS), gel permeation chromatography-multiangle laser light scattering (GPC-MALLS), and elemental analysis. The results of electrophoretic analysis suggest that the Zn complexes bound to DNA by means of electrostatic interactions and intercalation. The effect of the Zn complexes on the growth of SMMC-7721 liver cancer cells was investigated by sulforhodamine B assay in vitro. The results reveal that the growth of liver cancer cells was inhibited by LMW-chitosan and their Zn complexes. The inhibition rate of the Zn complexes was higher than that of LMW-chitosan ligand. The LMW-chitosan Schiff-base Zn complex exhibited higher anticancer activity than the LMW-chitosan Zn complex. Combining LMW-chitosan with Schiff-base and Zn improved its anticancer activity, which we ascribe to the synergistic effect between the chitosan matrix and the planar construction of the Zn complexes.

Photodynamic Anticancer Activity of CoFe2O4 Nanoparticles Conjugated with Hematoporphyrin

2015 ◽  
Vol 15 (10) ◽  
pp. 7900-7906 ◽  
Author(s):  
Bong Joo Park ◽  
Kyong-Hoon Choi ◽  
Ki Chang Nam ◽  
Jeeeun Min ◽  
Kyu-Dong Lee ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Cobalt Ferrite ◽  
Magnetic Core ◽  
Water Soluble ◽  
Anticancer Activities ◽  
Good Biocompatibility

This work reports the synthesis and the characterization of water-soluble and biocompatible photosensitizer (PS)-conjugated magnetic nanoparticles composed of a cobalt ferrite (CoFe2O4) magnetic core coated with a biocompatible hematoporphyrin (HP) shell. The photo-functional cobalt ferrite magnetic nanoparticles (CoFe2O4@HP) were uniform in size, stable against PS leaching, and highly efficient in the photo-generation of cytotoxic singlet oxygen under visible light. With the CoFe2O4@HP, we acquired in vitro MR images of cancer cells (PC-3) and confirmed good biocompatibility of the CoFe2O4@HP in both normal and cancer cells. In addition, we confirmed the potential of the CoFe2O4@HP as an agent for photodynamic therapy (PDT) applications. The photodynamic anticancer activities in 25, 50, and 100 μg/mL of CoFe2O4@HP were measured and found to exceed 99% (99.0, 99.4, and 99.5%) (p <0.002). The photodynamic anticancer activity was 81.8% (p < 0.003). From these results, we suggest that our CoFe2O4@HP can be used safely as a type of photodynamic cancer therapy with potential as a therapeutic agent having good biocompatibility. Moreover, these photo-functional magnetic nanoparticles are highly promising for applications in versatile imaging diagnosis and as a therapy tool in biomedical engineering.

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