Overexpression of Triticum durum TdAnn12 gene confers stress tolerance through scavenging reactive oxygen species in transgenic tobacco

2019 ◽  
Vol 46 (10) ◽  
pp. 885 ◽  
Author(s):  
Rania Ben Saad ◽  
Marwa Harbaoui ◽  
Walid Ben Romdhane ◽  
Nabil Zouari ◽  
Khong N. Giang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stresses ◽  
Triticum Durum ◽  
Antioxidant Activities ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Stress Related Genes ◽  
Triticum Durum Desf

Plant annexins are proteins with multiple functions and roles in plant development and responses to abiotic stresses. We report here the functional analysis of the TdAnn12 annexin protein isolated from Triticum durum Desf. We have previously shown that TdAnn12 expression is highly induced by different abiotic stresses. In the present study, to investigate the physiological and biochemical stress-induced responses, we overexpressed TdAnn12 in tobacco. We demonstrate that transgenic tobacco plants expressing TdAnn12 exhibited enhanced tolerance to salt, osmotic stress and H2O2 at the seedling stage. Under greenhouse conditions, these plants showed tolerance to drought and salt stresses. Moreover, scavenging reactive oxygen species (ROS), higher chlorophyll content, lower lipid peroxidation levels and increased antioxidant activities (peroxidase, catalase and superoxide dismutase) were observed. Finally, accumulation of TdAnn12 in tobacco positively affects the regulation of some stress-related genes (MnSOD, APX1, CAT1, P5CS, NHX1, SOS1 and DREB1A). TdAnn12 interacts directly or indirectly with stress-related genes that could stimulate an adaptive potential to gain tolerance which is not present in non-transgenic (NT) plants. Our results clearly show that overexpression of TdAnn12 in transgenic tobacco improves stress tolerance through the removal of ROS.

scholarly journals Biostimulants for the Regulation of Reactive Oxygen Species Metabolism in Plants under Abiotic Stress

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2537
Author(s):  
Mirza Hasanuzzaman ◽  
Khursheda Parvin ◽  
Kirti Bardhan ◽  
Kamrun Nahar ◽  
Taufika Islam Anee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Antioxidant Defense ◽  
Reactive Oxygen ◽  
Antioxidant Defense System ◽  
Oxygen Species ◽  
Defense System ◽  
Multiple Stress

Global food security for a growing population with finite resources is often challenged by multiple, simultaneously occurring on-farm abiotic stresses (i.e., drought, salinity, low and high temperature, waterlogging, metal toxicity, etc.) due to climatic uncertainties and variability. Breeding for multiple stress tolerance is a long-term solution, though developing multiple-stress-tolerant crop varieties is still a challenge. Generation of reactive oxygen species in plant cells is a common response under diverse multiple abiotic stresses which play dual role of signaling molecules or damaging agents depending on concentration. Thus, a delicate balance of reactive oxygen species generation under stress may improve crop health, which depends on the natural antioxidant defense system of the plants. Biostimulants represent a promising type of environment-friendly formulation based on natural products that are frequently used exogenously to enhance abiotic stress tolerance. In this review, we illustrate the potential of diverse biostimulants on the activity of the antioxidant defense system of major crop plants under stress conditions and their other roles in the management of abiotic stresses. Biostimulants have the potential to overcome oxidative stress, though their wider applicability is tightly regulated by dose, crop growth stage, variety and type of biostimulants. However, these limitations can be overcome with the understanding of biostimulants’ interaction with ROS signaling and the antioxidant defense system of the plants.

scholarly journals How to express the antioxidant properties of substances properly?

2021 ◽  
Author(s):  
Małgorzata Olszowy-Tomczyk
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Literature Review ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reactive Oxygen ◽  
The Body ◽  
Universal Method ◽  
Oxygen Species ◽  
Good Tool

AbstractOxidative stress, associated with an imbalance between the oxidants (reactive oxygen species) and the antioxidants in the body, contributes to the development of many diseases. The body’s fight against reactive oxygen species is supported by antioxidants. Nowadays, there are too many analytical methods, but there is no one universal technique for assessing antioxidant properties. Moreover, the applied different ways of expressing the results lead to their incompatibility and unreasonable interpretation. The paper is a literature review concerning the most frequent ways of antioxidant activities expression and for an easy and universal method of the obtained results discussion. This paper is an attempt to point out their disadvantages and advantages. The manuscript can support the searching interpretation of the obtained results which will be a good tool for the development of a number of fields, especially medicine what can help in the future detection and treatment of many serious diseases. Graphic abstract

Role of reactive oxygen species in the regulation of abiotic stress tolerance in legumes

2021 ◽  
pp. 217-243
Author(s):  
Ashutosh Sharma ◽  
Pooja Sharma ◽  
Rahul Kumar ◽  
Vikas Sharma ◽  
Renu Bhardwaj ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Reactive Oxygen ◽  
Oxygen Species

SbMYB15 transcription factor mitigates cadmium and nickel stress in transgenic tobacco by limiting uptake and modulating antioxidative defence system

2019 ◽  
Vol 46 (8) ◽  
pp. 702 ◽  
Author(s):  
Komal K. Sapara ◽  
Jackson Khedia ◽  
Parinita Agarwal ◽  
Doddabhimappa R. Gangapur ◽  
Pradeep K. Agarwal
Keyword(s):  
Reactive Oxygen Species ◽  
Heavy Metal ◽  
Transgenic Tobacco ◽  
Cell Fate ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Reactive Oxygen ◽  
Transcript Expression ◽  
Oxygen Species ◽  
Salicornia Brachiata

Plants require different inorganic minerals in an appropriate amount for growth; however, imbalance can limit growth and productivity. Heavy metal accumulation causes toxicity and generates signalling crosstalk with reactive oxygen species (ROS), phytohormones, genes and transcription factors (TFs). The MYB (myeloblastoma) TFs participate in plant processes such as metabolism, development, cell fate, hormone pathways and responses to stresses. This is the first report towards characterisation of R2R3-type MYB TF, SbMYB15, from succulent halophyte Salicornia brachiata Roxb. for heavy metal tolerance. The SbMYB15 showed >5-fold increased transcript expression in the presence of CdCl2 and NiCl2•6H2O. The constitutive overexpression of SbMYB15 conferred cadmium and nickel tolerance in transgenic tobacco, with improved growth and chlorophyll content. Further, the transgenics showed reduced generation of reactive oxygen species (H2O2 and O2•−) as compared with the wild-type (WT) with both Cd2+ and Ni2+ stress. Transgenics also showed low uptake of heavy metal ions, increased scavenging activity of the antioxidative enzymes (CAT and SOD) and higher transcript expression of antioxidative genes (CAT1 and MnSOD). Thus, the present study signifies that SbMYB15 can be deployed for developing heavy metal tolerance in crop plants via genetic engineering.

scholarly journals Reactive Oxygen Species Are Involved in Brassinosteroid-Induced Stress Tolerance in Cucumber

2009 ◽  
Vol 150 (2) ◽  
pp. 801-814 ◽  
Author(s):  
Xiao-Jian Xia ◽  
Yan-Jie Wang ◽  
Yan-Hong Zhou ◽  
Yuan Tao ◽  
Wei-Hua Mao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Stress Tolerance ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Induced Stress
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