Effects of progesterone withdrawal on uterine secretion of prostaglandin F2α in response to oxytocin in ewes

1997 ◽  
Vol 9 (2) ◽  
pp. 255 ◽  
Author(s):  
M. A. Kaminski ◽  
S. H. Hayes ◽  
W. J. Silvia
Keyword(s):  
Venous Blood ◽  
Prostaglandin F2α ◽  
Inhibitory Effect ◽  
Treatment Groups ◽  
Group 4 ◽  
Sham Surgery ◽  
Group 3 ◽  
Group 2 ◽  
Ovine Uterus ◽  
Group 1

Two experiments were conducted to determine if withdrawal of progesterone during the luteal phase of the oestrous cycle affected the ability of the ovine uterus to secrete prostaglandin F2α(PGF2α ) in response to oxytocin. In Experiment 1, 18 ewes were ovariectomized on Day 9 and Day 12 after oestrus. Ewes were subdivided into three treatment groups (n= 6 per group): Group-1 ewes underwent sham surgery; Group-2 ewes received oestradiol (OVX + O); and Group-3 ewes received oestradiol + progesterone (OVX + O,P). Oxytocin was administered to each ewe on Days 10, 13 and 15 after oestrus. Concentrations of 13,14-dihydro-15-keto-PGF2α (PGFM) were determined in samples of jugular venous blood for 2 h after oxytocin challenge. The magnitude of the PGFM response 24 h after ovariectomy was greater (P < 0·1) in ewes from which progesterone had been withdrawn (OVX + O) than in ewes in which progesterone was maintained (intact controls and OVX + O,P). Therefore, progesterone appears to exert an inhibitory effect on uterine secretory responsiveness to oxytocin which is removed by progesterone withdrawal. In Experiment 2, ewes were ovariectomized on Day 11 and assigned to 1 of 4 treatment groups (n = 6 per group): Group 1, no steroid replacement (OVX); Group 2, oestradiol replacement (OVX + O); Group 3, progesterone replacement (OVX + P); or Group 4, progesterone+oestradiol replacement (OVX + O,P). Ewes received oxytocin on Day 12 and Day 15. On Day 12, uterine secretory responsiveness to oxytocin was greatest in ewes in the OVX + O group (P < 0 · 1). Responsiveness was low in ewes in the OVX group, as it was in ewes in both groups that received progesterone replacement. Therefore, the increase in uterine secretory responsiveness to oxytocin following progesterone withdrawal is dependent on oestradiol replacement.

scholarly journals Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus)

2018 ◽  
Vol 24 (1) ◽  
pp. 37
Author(s):  
Annisa Trissatharra ◽  
Sri Ratna Dwiningsih ◽  
Ratna Sofaria Munir
Keyword(s):  
Mus Musculus ◽  
Ovarian Follicles ◽  
Observation Time ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.

scholarly journals Investigation of the protective effect of enoxaparin and ticagrelor pretreatment against ischemia-reperfusion injury in rat lung tissue

2019 ◽  
Vol 65 (9) ◽  
pp. 1193-1200
Author(s):  
Orhan Fındık ◽  
Melda Yardımoglu Yılmaz ◽  
Yusufhan Yazır ◽  
Selenay Furat Rençber ◽  
Kübra Kavram Sarıhan ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Caspase 3 ◽  
Ischemia Reperfusion ◽  
Histological Structure ◽  
Apoptotic Cells ◽  
Treatment Groups ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

SUMMARY OBJECTIVES This study was conducted to reveal the possible protective effects of ticagrelor and enoxaparin pretreatment against ischemia-reperfusion (IR)-induced injury on the lung tissue of a rat model. METHODS Wistar albino rats were randomly divided into 4 groups as follows: group-1 (control-sham), group-2 (control-saline+IR), group-3 (ticagrelor+IR), group-4 (enoxaparin+IR). Before the ischemic period, saline, ticagrelor, and enoxaparin were administered to the 2nd-4th groups, respectively. In these groups, IR injury was induced by clamping the aorta infrarenally for 2 h, followed by 4 h of reperfusion except group-1. After the rats were euthanized, the lungs were processed for histological examinations. Paraffin sections were stained with Haematoxylin&Eosin (H&E) for light microscopic observation. Apoptosis was evaluated by caspase-3 immunoreactivity. Data were statistically analyzed using the SPSS software. RESULTS In the lung sections stained with H&E, a normal histological structure was observed in group-1, whereas disorganized epithelial cells, hemorrhage, and inflammatory cell infiltration were seen in the alveolar wall in group-2. The histologic structure of the treatment groups was better than that of group-2. Caspase-3(+) apoptotic cells were noticeable in sections of group-2 and were lower in the treatment groups. In group-4, caspase-3 immunostaining was lower than in group-3. In group-2, apoptotic cells were significantly higher than in the other groups (p<0.001). CONCLUSION Based on the histological results, we suggested that both therapies ameliorated the detrimental effects of IR. Caspase-3 immunohistochemistry results also revealed that pre-treatment with enoxaparin gave better results in an IR-induced rat injury model. In further studies, other parameters such as ROS and inflammatory gene expressions should be evaluated for accurate results.

scholarly journals Effect of two prostaglandin F2α injections administered 24 hours apart on the pregnancy rate of Simmental cows subjected to the Ovsynch or Ovsynch + Controlled internal drug release (CIDR) protocols

2020 ◽  
Vol 76 (10) ◽  
pp. 6451-2020
Author(s):  
MUSHAP KURU ◽  
CIHAN KACAR ◽  
HASAN ORAL ◽  
SEMRA KAYA ◽  
NEBI CETIN ◽  
...  
Keyword(s):  
Drug Release ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Pregnancy Loss ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

The aim of the present study was to determine the effects of one or two doses of prostaglandin F2α (PGF) on the pregnancy rate (PR) in Simmental cows subjected to the Ovsynch or Ovsynch + Controlled internal drug release (CIDR) protocols. On d 0, 100 µg gonadorelin diacetate tetrahydrate (GnRH) was injected into cows in group 1 (n = 50) and group 2 (n = 40) and CIDR was intravaginally inserted. On d 7, 25 mg dinoprost tromethamine (PGF2α, PGF) was administered, and GnRH was injected 56 h later. Fixed-time artificial insemination (TAI) was performed 16 h after GnRH injection. In group 3 (n = 62) and group 4 (n = 63), PGF was administered 7 d following GnRH treatment, and GnRH was injected 56 h later. TAI was performed 16 h after GnRH injection. In groups 2 and 4, a second dose of PGF was injected 24 h after the first dose. Pregnancy was determined by transrectal ultrasonography on d 30 and 60 post-TAI. The pregnancy rate (PR) on d 30 post-TAI was significantly different among the groups: 46%, 55%, 29%, and 36.5%, in groups 1, 2, 3, and 4, respectively (P = 0.048). In addition, the PR was 36.3% and 43.7% in the single PGF and double PGF injection groups, respectively. There was no statistical difference in the PR on d 60 post-TAI (P > 0.05). Pregnancy loss was between 4.34% and 11.11% (P > 0.05). The highest PR in the primiparous cows was observed in group 3 (57.7%). The PR was not significantly different among multiparous cows (P > 0.05). In conclusion, two injections of PGF administered 24 h apart in Simmental cows may increase the PR in the Ovsynch or Ovsynch + CIDR protocols.

scholarly journals EFEK PENYUNTIKAN PROSTAGLANDIN F2α (PGF2α) TERHADAP TIMBUL DAN LAMA BIRAHI SAPI BALI PADA PARITAS YANG BERBEDA

2019 ◽  
Vol 44 (2) ◽  
pp. 141
Author(s):  
Abdul Malik
Keyword(s):  
Single Dose ◽  
Prostaglandin F2α ◽  
Estrus Synchronization ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Estrus Response ◽  
Group 2 ◽  
Time Required ◽  
Group 1

The objective of this study was to evaluate of Effect of injection Prostaglandin F2α on the time required for onset of estrus and estrus durations in Bali cattle with differences of Paritas. A total of 24 Bali cows were divided into four groups, each group was consisting of 6 cows.  Group 1 samples is heifer’s, group 2, samples was paritas1, group 3, samples was paritas 2 and group 4, samples was paritas 3.  All  cows were normal  tract of reproduction  with showing regular cyclic activities and having active of corpus luteum. The parameters in this study were determined including the time required for onset of estrus and duration of estrus. The result showed that percentage estrus response and duration of estrus were non-significant difference (P>0.05) between heifers and paritas 1, 2, and 3 after estrus synchronization with single dose of prostaglandin F2α. It was concluded that injection of PGF2α on heifers and Paritas 1,2 and 3 were  similar result on the time required for onset of estrus and duration of estrus.

scholarly journals Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

2010 ◽  
Vol 25 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Marco Antonio de Oliveira Filho ◽  
Paulo Afonso Nunes Nassif ◽  
Osvaldo Malafaia ◽  
Jurandir Marcondes Ribas Filho ◽  
Carmen Austrália Paredes Marcondes Ribas ◽  
...  
Keyword(s):  
Bone Repair ◽  
Platelet Rich Plasma ◽  
Blood Clot ◽  
Venous Blood ◽  
Control Group ◽  
Advanced Stage ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

PURPOSE: To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. METHODS: The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th and 6th week postoperatively. Histological and histomorphometric analysis were carried through. RESULTS: After two weeks, groups 1 and 2 were in more advanced stage of repair than 3 and 4. At this period, comparing the groups 1 and 2, no significant differences were found between both, which also happened between the groups 3 and 4. However, after four and six weeks, the group 1 showed a more advanced stage of repair among all the other studied groups, while group 2 was in more advanced signs of bone repair than groups 3 and 4. Comparing groups 3 and 4, after four and six weeks, the least advanced stage of bone repair was found to be within group 3. CONCLUSION: The use of a highly concentrated PRP was considered prejudicial to the repair of non-critical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot (control).

Ontogeny of black-footed ferret predatory behavior towards prairie dogs

1998 ◽  
Vol 76 (9) ◽  
pp. 1696-1704 ◽  
Author(s):  
Astrid Vargas ◽  
Stanley H Anderson
Keyword(s):  
Predatory Behavior ◽  
Prairie Dogs ◽  
Live Prey ◽  
Mustela Nigripes ◽  
Treatment Groups ◽  
Group 4 ◽  
Predation Efficiency ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

To study the effects of environmental upbringing and predation experience on black-footed ferret (Mustela nigripes) predatory skills towards prairie dogs, we compared killing efficiency and behaviors of 32 black-footed ferret kits (from 24 different litters). Four treatment groups were established: group 1 (n = 8), kits raised in indoor cages and never exposed to live prey; group 2 (n = 8), kits raised in indoor cages and fed live hamsters twice a week beginning at 8 weeks of age; group 3 (n = 8), kits raised in indoor cages, fed live hamsters, and exposed to live prairie dogs in family trials once per week; and group 4, kits raised in outdoor enclosures with ad libitum access to live prairie dogs in natural burrows. Juvenile black-footed ferrets raised with exposure to live hamsters were more successful at killing prairie dogs than kits devoid of any predation experience. Previous experience with prairie dogs (in both indoor cages and outdoor pens) significantly increased predation efficiency. Witnessing a black-footed ferret mother performing a kill enhanced the kit's predatory skills. We conclude that the placement of the kill bite appears to be innate for black-footed ferrets, but the likelihood of killing and the efficiency at handling prey are substantially enhanced by experience.

THE EFFECTS OF TIME OF INSEMINATION ON FERTILITY IN BEEF HEIFERS SYNCHRONIZED WITH PROSTAGLANDIN F2α

1977 ◽  
Vol 57 (1) ◽  
pp. 47-51 ◽  
Author(s):  
J. G. MANNS ◽  
M. S. WENKOFF ◽  
W. M. ADAMS ◽  
G. RICHARDSON
Keyword(s):  
Prostaglandin F2α ◽  
Corpora Lutea ◽  
Beef Heifers ◽  
Serum Progesterone ◽  
Group 4 ◽  
Prostaglandin F ◽  
Group 3 ◽  
Group 2 ◽  
Observation Period ◽  
Group 1

Two injections of prostaglandin F2α (PGF2α) spaced 12 days apart were used to synchronize estrus in Hereford heifers. Animals were inseminated at 75 h (group 2), 80 h (group 3) or 85 h (group 4) after the second injection of PGF2α. Untreated control animals (group 1) were inseminated as detected in estrus over an observation period of approximately 35 days. Immediately before, and 24 h after each PGF2α injection, blood was collected for progesterone assay. Fertility expressed as calving rates was as follows: group 1, 33/77 (43%); group 2, 30/79 (38%); group 3, 29/79 (37%); group 4, 20/73 (27%). Fertility was depressed at 85 h vs. control (P < 0.05) in PGF2α-treated animals but there were no other significant differences. Progesterone assays showed that 65% of animals had progesterone-secreting corpora lutea at the first injection of PGF2α. There was no relationship between fertility and either serum progesterone concentration or the day of the cycle at the second injection of PGF2α.

In-vivo evaluation of the effect of cyanoacrylate on prosthetic vascular graft infection – does cyanoacrylate increase the severity of infection?

VASA ◽  
2020 ◽  
Vol 49 (4) ◽  
pp. 281-284
Author(s):  
Atıf Yolgosteren ◽  
Gencehan Kumtepe ◽  
Melda Payaslioglu ◽  
Cuneyt Ozakin
Keyword(s):  
Vascular Graft ◽  
Graft Infection ◽  
Vascular Graft Infection ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Prosthetic Vascular Graft Infection ◽  
Group 1

Summary. Background: Prosthetic vascular graft infection (PVGI) is a complication with high mortality. Cyanoacrylate (CA) is an adhesive which has been used in a number of surgical procedures. In this in-vivo study, we aimed to evaluate the relationship between PVGI and CA. Materials and methods: Thirty-two rats were equally divided into four groups. Pouch was formed on back of rats until deep fascia. In group 1, vascular graft with polyethyleneterephthalate (PET) was placed into pouch. In group 2, MRSA strain with a density of 1 ml 0.5 MacFarland was injected into pouch. In group 3, 1 cm 2 vascular graft with PET piece was placed into pouch and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. In group 4, 1 cm 2 vascular graft with PET piece impregnated with N-butyl cyanoacrylate-based adhesive was placed and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. All rats were scarified in 96th hour, culture samples were taken where intervention was performed and were evaluated microbiologically. Bacteria reproducing in each group were numerically evaluated based on colony-forming unit (CFU/ml) and compared by taking their average. Results: MRSA reproduction of 0 CFU/ml in group 1, of 1410 CFU/ml in group 2, of 180 200 CFU/ml in group 3 and of 625 300 CFU/ml in group 4 was present. A statistically significant difference was present between group 1 and group 4 (p < 0.01), between group 2 and group 4 (p < 0.01), between group 3 and group 4 (p < 0.05). In terms of reproduction, no statistically significant difference was found in group 1, group 2, group 3 in themselves. Conclusions: We observed that the rate of infection increased in the cyanoacyrylate group where cyanoacrylate was used. We think that surgeon should be more careful in using CA in vascular surgery.

Does Perioperative Hemoglobin A1c Level Affect the Incidence, Pattern and Mortality of Lower Extremity Amputation?

2019 ◽  
Vol 17 (4) ◽  
pp. 354-364
Author(s):  
Hassan Al-Thani ◽  
Moamena El-Matbouly ◽  
Maryam Al-Sulaiti ◽  
Noora Al-Thani ◽  
Mohammad Asim ◽  
...  
Keyword(s):  
Glycemic Control ◽  
Lower Extremity ◽  
Hazard Ratio ◽  
Lower Extremity Amputation ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Extremity Amputation ◽  
Group 1

Background: We hypothesized that perioperative HbA1c influenced the pattern and outcomes of Lower Extremity Amputation (LEA). Methods: A retrospective analysis was conducted for all patients who underwent LEA between 2000 and 2013. Patients were categorized into 5 groups according to their perioperative HbA1c values [Group 1 (<6.5%), Group 2 (6.5-7.4%), Group 3 (7.5-8.4%), Group 4 (8.5-9.4%) and Group 5 (≥9.5%)]. We identified 848 patients with LEA; perioperative HbA1c levels were available in 547 cases (Group 1: 18.8%, Group 2: 17.7%, Group 3: 15.0%, Group 4: 13.5% and Group 5: 34.9%). Major amputation was performed in 35%, 32%, 22%, 10.8% and 13.6%, respectively. Results: The overall mortality was 36.5%; of that one quarter occurred during the index hospitalization. Mortality was higher in Group 1 (57.4%) compared with Groups 2-5 (46.9%, 38.3%, 36.1% and 31.2%, respectively, p=0.001). Cox regression analysis showed that poor glycemic control (Group 4 and 5) had lower risk of mortality post-LEA [hazard ratio 0.57 (95% CI 0.35-0.93) and hazard ratio 0.46 (95% CI 0.31-0.69)]; this mortality risk persisted even after adjustment for age and sex but was statistically insignificant. The rate of LEA was greater among poor glycemic control patients; however, the mortality was higher among patients with tight control. Conclusion: The effects of HbA1c on the immediate and long-term LEA outcomes and its therapeutic implications need further investigation.

Association between cervical disc disease and lesions of multiple sclerosis

2021 ◽  
pp. 197140092098356
Author(s):  
Marwan Alkrenawi ◽  
Michael Osherov ◽  
Azaria Simonovich ◽  
Jonathan Droujin ◽  
Ron Milo ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Cervical Disc ◽  
Cervical Disc Disease ◽  
Disc Disease ◽  
Group 4 ◽  
Demyelinating Lesions ◽  
Grade Ii ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Background Cervical discopathy and demyelinating lesions often co-exist in patients with multiple sclerosis (MS). Our study examines the possible association between these two pathologies. Methods Medical records and cervical magnetic resonance imaging scans of MS patients with cervical discopathy who were seen at our MS clinic during 2018 were retrospectively reviewed. The severity of the disc disease was classified as grade I (no compression), grade II (compression of the dural sac) and grade III (cord compression). The spinal cord in each scan was divided into six segments corresponding to the intervertebral space of the spine (C1–C6). Each segment was defined as containing demyelinating lesion and disc pathology (group 1), demyelinating lesion without disc pathology (group 2), disc pathology without demyelinating lesion (group 3) and no demyelinating lesion or disc pathology (group 4). Fisher’s exact test was used to test the association between demyelinating lesions and disc pathology. Results Thirty-four MS patients with cervical discopathy were included in the study (26 females; average age 42.9 ± 13.7 years; average disease duration 8.4 ± 5.4 years). A total of 204 spinal cord segments were evaluated. Twenty-four segments were classified as group 1, 27 segments as group 2, 52 segments as group 3 and 101 segments as group 4. There was no association between demyelinating lesions and the grade of disc disease ( p = 0.1 for grade I, p = 0.3 for grade II and p = 1 for grade III disc disease). Conclusion Our study did not find any association between cervical disc disease and demyelinating spinal cord lesion.

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