Mucin 15 is lost but mucin 13 remains in uterine luminal epithelial cells and the blastocyst at the time of implantation in the rat

2014 ◽  
Vol 26 (3) ◽  
pp. 421 ◽  
Author(s):  
Connie E. Poon ◽  
Laura Lecce ◽  
Margot L. Day ◽  
Christopher R. Murphy
Keyword(s):  
Epithelial Cells ◽  
Apical Cell ◽  
Cell Signalling ◽  
Ovarian Hormones ◽  
Hormonal Control ◽  
Luminal Epithelium ◽  
Uterine Receptivity ◽  
Molecular Weights ◽  
Luminal Epithelial Cells ◽  
Adhesive Capacity

The glycocalyx of the uterine luminal epithelium in the rat undergoes considerable reduction before implantation. In particular, the reduction of some mucins is necessary to facilitate blastocyst adhesion and subsequent implantation. The present study investigated the localisation, abundance and hormonal control of two mucin proteins, Muc13 and Muc15, in rat uterine epithelial cells during early pregnancy to determine whether they are likely to play a role in uterine receptivity for implantation. Muc13 and Muc15 are localised to the uterine luminal epithelium but show a presence and an absence, respectively, at the apical cell surface at the time of implantation. This localisation corresponds to changes in the molecular weights of Muc13 and Muc15, as shown with western blotting analysis. Furthermore, the localisation of Muc13 and Muc15 was shown to be controlled by the ovarian hormones, oestrogen and progesterone, and they were also localised in preimplantation rat blastocysts. Our results suggest that Muc15 may operate in an anti-adhesive capacity to prevent implantation while Muc13 potentially functions in either an adhesive or cell-signalling role in the events of implantation.

Progestin increases cathepsin D synthesis in uterine luminal epithelial cells

1983 ◽  
Vol 244 (5) ◽  
pp. E442-E446 ◽  
Author(s):  
B. C. Moulton ◽  
B. B. Koenig
Keyword(s):  
Epithelial Cells ◽  
Cathepsin D ◽  
Hormonal Control ◽  
Ovariectomized Rats ◽  
Luminal Epithelium ◽  
Progesterone Secretion ◽  
Blastocyst Implantation ◽  
Lysosomal Proteinase ◽  
Cathepsin D Activity ◽  
Luminal Epithelial Cells

Early in blastocyst implantation, cells of the uterine luminal epithelium deteriorate and die in response to the presence of the blastocyst. Destruction of the epithelial cells appears to depend on control of the autophagic activity and enzyme content of lysosomes in these cells. Concentrations of the lysosomal proteinase, cathepsin D, have been identified in luminal epithelial cells, and these studies examined changes in epithelial cathepsin D activity and their hormonal control during early pseudopregnancy in the rat. Cathepsin D activity in luminal epithelial cells increases during early pseudopregnancy to maximal levels at the time of sensitivity to deciduogenic stimuli. Rates of cathepsin D synthesis in luminal epithelial cells also increase during early pseudopregnancy, but neither enzyme activity nor rates of synthesis increase in stromal-myometrial tissues. In ovariectomized rats, progestins rather than estradiol increase cathepsin D activity and rates of synthesis in luminal epithelial cells. These studies suggest that cell death in the luminal epithelium during blastocyst implantation may depend in part on the accumulation of lysosomal cathepsin D in these cells in response to progesterone secretion during early pregnancy.

scholarly journals [Ciliogenesis in the mucous cells of the quail oviduct. II. Hormonal control]

1976 ◽  
Vol 71 (2) ◽  
pp. 460-471 ◽  
Author(s):  
D Sandoz ◽  
E Biosvieux-Ulrich ◽  
C Laugier ◽  
E Brard
Keyword(s):  
Epithelial Cells ◽  
Estradiol Benzoate ◽  
Hormonal Control ◽  
Secretory Cells ◽  
Luminal Epithelium ◽  
Mucous Cells ◽  
Ciliated Cells ◽  
Cell Divisions ◽  
Progesterone Treatment

The hormonal control of ciliogenesis and transformation of mucous cells was studied in the oviduct (magnum) of ovariectomized quails. Estradiol benzoate induces ciliogenesis with doses varying from 10 mug/day to 100 mug/day after 6 days of treatment. With 100 mug/day, differentiation of some mucous cells is also induced as well as the formation of transitory "mixed cells" which are in the process of ciliogenesis and contain mucous granules. Associated with progesterone (1 mg/day), estradiol benzoate (10 mug/day) induces the differentiation of mucous cells and ciliated cells. The luminal epithelium of quails injected with this mixture is similar to the luminal epithelium observed in the oviduct of laying quails. With the same dose of progesterone (1 mg/day) and 20 mug/day of estradiol benzoate for 6 days, ciliogenesis is completely inhibited. All epithelial cells are secretory cells. Transformation of 50% of the mucous cells into ciliated cells is obtained by following the previous estradiol-progesterone treatment with the injection of estradiol benzoate (20 mug/day) for 3 days. Divisions of mucous cells were also observed. It is also possible to induce ciliogenesis in some mucous cells by withdrawing both hormones for 3 days. In this case, no cell divisions were observed.

238. Claudins and occludin in the rat endometrium

2005 ◽  
Vol 17 (9) ◽  
pp. 94
Author(s):  
M. D. O. Nicholson ◽  
C. R. Murphy
Keyword(s):  
Epithelial Cells ◽  
Tight Junctions ◽  
Early Pregnancy ◽  
Immunofluorescence Microscopy ◽  
Ovarian Hormones ◽  
Cellular Distribution ◽  
Luminal Epithelium ◽  
Uterine Lumen ◽  
Uterine Glands ◽  
Ovariectomised Rats

Regulation of the uterine luminal environment is important for the successful attachment and implantation of the blastocyst. Tight junctions regulate the paracellular pathway between epithelial cells lining the uterine lumen and the uterine glands. The aims of this present study was firstly to establish the presence and cellular distribution of claudins and occludin in the luminal epithelia during early pregnancy using immunofluorescence microscopy and deconvolution, and secondly to determine the influence of ovarian hormones on their expression. Occludin and claudins -1, -3, and -4 were present in luminal epithelium. Occludin and claudin-4 showed increased expression in luminal epithelium at the time of implantation, whereas claudin-1 and -3 expression remained the same throughout early pregnancy. In ovariectomised rats administered ovarian hormones, occludin and claudin-4 showed increased expression in luminal epithelium in progesterone-dominant regimes and decreased expression when administered oestrogen alone. Expression of claudin-1 and -3 in luminal epithelium was not effected by ovarian hormones. Claudin-2 was not expressed during early pregnancy nor in ovariectomised rats. In conclusion, these results show that occludin and claudins -1, -3 and -4 are present in luminal and glandular epithelium, and provide the permeability properties needed to separate the luminal and the stromal environment at the time of implantation. Furthermore, occludin and claudin-4 expression is controlled by ovarian hormones being upregulated by progesterone.

scholarly journals Nucleolar stress regulation of endometrial receptivity in mouse models and human cell lines

2019 ◽  
Vol 10 (11) ◽  
Author(s):  
Wei Hu ◽  
Yu-Xiang Liang ◽  
Jia-Mei Luo ◽  
Xiao-Wei Gu ◽  
Zi-Cong Chen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Early Pregnancy ◽  
Embryo Implantation ◽  
Uterine Receptivity ◽  
Positive Effects ◽  
Delayed Implantation ◽  
Nucleolar Stress ◽  
Pregnant Mice ◽  
Successful Establishment ◽  
Luminal Epithelial Cells

Abstract Embryo implantation is essential to the successful establishment of pregnancy. A previous study has demonstrated that actinomycin D (ActD) could initiate the activation of mouse delayed implantation. However, the mechanism underlying this activation remains to be elucidated. A low dose of ActD is an inducer of nucleolar stress. This study was to examine whether nucleolar stress is involved in embryo implantation. We showed that nucleolar stress occurred when delayed implantation was activated by ActD in mice. ActD treatment also stimulated the Lif-STAT3 pathway. During early pregnancy, nucleolar stress was detected in the luminal epithelial cells during the receptive phase. Blastocyst-derived lactate could induce nucleolar stress in cultured luminal epithelial cells. The inhibition of nucleophosmin1 (NPM1), which was a marker of nucleolar stress, compromised uterine receptivity and decreased the implantation rates in pregnant mice. To translate these mouse data into humans, we examined nucleolar stress in human endometrium. Our data demonstrated that ActD-induced nucleolar stress had positive effects on the embryo attachment by upregulating IL32 expression in non-receptive epithelial cells rather than receptive epithelial cells. Our data should be the first to demonstrate that nucleolar stress is present during early pregnancy and is able to induce embryo implantation in both mice and humans.

scholarly journals Alkaline phosphatases contribute to uterine receptivity, implantation, decidualization, and defense against bacterial endotoxin in hamsters

Reproduction ◽  
2013 ◽  
Vol 146 (5) ◽  
pp. 419-432 ◽  
Author(s):  
Wei Lei ◽  
Heidi Nguyen ◽  
Naoko Brown ◽  
Hua Ni ◽  
Tina Kiffer-Moreira ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Epithelial Cells ◽  
Hormonal Regulation ◽  
Cell Type ◽  
Uterine Receptivity ◽  
Rt Pcr ◽  
Alkaline Phosphatases ◽  
Specific Expression ◽  
Luminal Epithelial Cells

Alkaline phosphatase (AP) activity has been demonstrated in the uterus of several species, but its importance in the uterus, in general and during pregnancy, is yet to be revealed. In this study, we focused on identifying AP isozyme types and their hormonal regulation, cell type, and event-specific expression and possible functions in the hamster uterus during the cycle and early pregnancy. Our RT-PCR and in situ hybridization studies demonstrated that among the known Akp2, Akp3, Akp5, and Akp6 murine AP isozyme genes, hamster uteri express only Akp2 and Akp6; both genes are co-expressed in luminal epithelial cells. Studies in cyclic and ovariectomized hamsters established that while progesterone (P4) is the major uterine Akp2 inducer, both P4 and estrogen are strong Akp6 regulators. Studies in preimplantation uteri showed induction of both genes and the activity of their encoded isozymes in luminal epithelial cells during uterine receptivity. However, at the beginning of implantation, Akp2 showed reduced expression in luminal epithelial cells surrounding the implanted embryo. By contrast, expression of Akp6 and its isozyme was maintained in luminal epithelial cells adjacent to, but not away from, the implanted embryo. Following implantation, stromal transformation to decidua was associated with induced expressions of only Akp2 and its isozyme. We next demonstrated that uterine APs dephosphorylate and detoxify endotoxin lipopolysaccharide at their sites of production and activity. Taken together, our findings suggest that uterine APs contribute to uterine receptivity, implantation, and decidualization in addition to their role in protection of the uterus and pregnancy against bacterial infection.

Ovarian hormones alter tight junction structure in uterine luminal epithelial cells

Micron (1969) ◽  
1980 ◽  
Vol 11 (3-4) ◽  
pp. 375-376 ◽  
Author(s):  
C.R. Murphy ◽  
A.W. Rogers ◽  
J.G. Swift ◽  
T.M. Mukherjee
Keyword(s):  
Epithelial Cells ◽  
Tight Junction ◽  
Ovarian Hormones ◽  
Junction Structure ◽  
Luminal Epithelial Cells

Autoradiographic localization of oxytocin receptors in the endometrium during the oestrous cycle of the ewe

1991 ◽  
Vol 130 (2) ◽  
pp. 199-NP ◽  
Author(s):  
V. J. Ayad ◽  
E. L. Matthews ◽  
D. C. Wathes ◽  
T. J. Parkinson ◽  
M. L. Wild
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Oxytocin Receptor ◽  
Oestrous Cycle ◽  
Luminal Epithelium ◽  
Tissue Samples ◽  
Luteal Regression ◽  
Oxytocin Receptors ◽  
Secretory Glands ◽  
Luminal Epithelial Cells

ABSTRACT The present study was designed to determine the localization of the endometrial oxytocin receptor during the ovine oestrous cycle, particularly on day 14, the time of initiation of luteal regression in the ewe. Samples were obtained from 29 ewes at different stages of the oestrous cycle (several during the luteal phase and on every day between day 14 (− 2) and day + 3 of the oestrous period). Oxytocin receptors were localized autoradiographically in sections of uterine tissue, using the 125I-labelled oxytocin receptor antagonist [1-(β-mercapto-β,β-cyclopentamethylene propionic acid), 2-(ortho-methyl)-Tyr2,Thr4,Orn8,Tyr9-NH2]-vasotocin (125I-labelled OTA). There was some variation in the pattern of 125I-labelled OTA labelling between different uterine tissue samples from the same ewe and also between samples obtained from different ewes thought to be at the same stage of the oestrous cycle. A clear overall pattern did, however, emerge with 125I-labelled OTA-binding sites distributed between luminal epithelial cells, glandular epithelial cells and caruncular stromal cells to varying extents on different days of the cycle. During the luteal phase (days 5–12) clear specific labelling of endometrial tissue was generally absent. On day 14 labelling was evident on the luminal epithelium, but only in nine tissue samples out of a total of 18 studied, indicating that the entire luminal surface did not contain oxytocin receptors at this time. Between the day before oestrus and day 3 of the oestrous cycle the luminal epithelium was consistently labelled. The most extensive labelling of the remaining endometrial tissue was observed on the day of oestrus, with 125I-labelled OTA-binding sites clearly present on the stromal cells within caruncles and on a large proportion of secretory epithelia. This contrasted with the day before and the day after oestrus when labelling of glandular tissue was confined to the superficial endometrium, and labelling of caruncular stromal cells, although sometimes evident, was never as intense as on day 0. On days 2 and 3 labelling varied between being similar to that found on day 1 and being confined to the luminal epithelium and very few superficial secretory glands. The results of this study lead us to conclude that the oxytocin receptor shows a differential distribution between stromal cells, epithelial cells lining secretory glands and luminal epithelial cells during the oestrous cycle; that the steroidal regulation of the oxytocin receptor differs between endometrial cell types; and that control of the luminal epithelial oxytocin receptors is probably of particular importance to the regulation of prostaglandin F2α release at luteal regression and during the maternal recognition of pregnancy. Journal of Endocrinology (1991) 130, 199–206

Platelet Microtubule Subunit Proteins

1979 ◽  
Vol 42 (05) ◽  
pp. 1630-1633 ◽  
Author(s):  
A G Castle ◽  
N Crawford
Keyword(s):  
Epithelial Cells ◽  
Gel Electrophoresis ◽  
Blood Platelets ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Lens Epithelial Cells ◽  
Molecular Weights ◽  
Kinetics Of ◽  
Microtubular Structures

SummaryBlood platelets contain microtubule proteins (tubulin and HMWs) which can be polymerised “in vitro” to form structures which resemble the microtubules seen in the intact platelet. Platelet tubulin is composed of two non-identical subunits a and p tubulin which have molecular weights around 55,000 but can be resolved in alkaline SDS-polyacrylamide gel electrophoresis. These subunits associate as dimers with sedimentation coefficients of about 5.7 S although it is not known whether the dimer protein is a homo- or hetero-dimer. The dimer tubulin binds the anti-mitotic drug colchicine and the kinetics of this binding are similar to those reported for neurotubulins. Platelet microtubules also contain two HMW proteins which appear to be essential and integral components of the fully assembled microtubule. These proteins have molecular weights greater than 200,000 daltons. Fluorescent labelled antibodies to platelet and brain tubulins stain long filamentous microtubular structures in bovine lens epithelial cells and this pattern of staining is prevented by exposing the cells to conditions known to cause depolymerisation of cell microtubules.

scholarly journals Highly metastatic claudin-low mammary cancers can originate from luminal epithelial cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Patrick D. Rädler ◽  
Barbara L. Wehde ◽  
Aleata A. Triplett ◽  
Hridaya Shrestha ◽  
Jonathan H. Shepherd ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Epithelial Cells ◽  
Triple Negative ◽  
Molecular Subtype ◽  
Ras Signaling ◽  
Preneoplastic Lesions ◽  
Independent Manner ◽  
Oncogenic Ras ◽  
Luminal Epithelial Cells

AbstractClaudin-low breast cancer represents an aggressive molecular subtype that is comprised of mostly triple-negative mammary tumor cells that possess stem cell-like and mesenchymal features. Little is known about the cellular origin and oncogenic drivers that promote claudin-low breast cancer. In this study, we show that persistent oncogenic RAS signaling causes highly metastatic triple-negative mammary tumors in mice. More importantly, the activation of endogenous mutant KRAS and expression of exogenous KRAS specifically in luminal epithelial cells in a continuous and differentiation stage-independent manner induces preneoplastic lesions that evolve into basal-like and claudin-low mammary cancers. Further investigations demonstrate that the continuous signaling of oncogenic RAS, as well as regulators of EMT, play a crucial role in the cellular plasticity and maintenance of the mesenchymal and stem cell characteristics of claudin-low mammary cancer cells.

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