Gene-specific profiling of DNA methylation and mRNA expression in bovine oocytes derived from follicles of different size categories

2017 ◽  
Vol 29 (10) ◽  
pp. 2040 ◽  
Author(s):  
F. Mattern ◽  
J. Heinzmann ◽  
D. Herrmann ◽  
A. Lucas-Hahn ◽  
T. Haaf ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mrna Expression ◽  
Candidate Genes ◽  
Growth Phase ◽  
Methylation Status ◽  
Developmental Competence ◽  
Follicular Growth ◽  
Antral Follicles ◽  
Follicle Diameter ◽  
Bovine Oocytes

Epigenetic changes, such as DNA methylation, play an essential role in the acquisition of full developmental competence by mammalian oocytes during the late follicular growth phase. Here we used the bovine model to investigate the DNA methylation profiles of seven candidate genes (imprinted: bH19, bSNRPN; non-imprinted: bZAR1, bDNMT3A, bOCT4, bDNMT3 Lo and bDNMT3 Ls) and the mRNA expression of nine candidate genes (imprinted: bSNRPN, bPEG3, bIGF2R; non-imprinted: bPRDX1, bDNMT1B, bDNMT3A, bZAR1, bHSF1 and bNLRP9) in oocytes from antral follicles of three different size classes (≤2 mm, 3–5 mm, ≥6 mm) to unravel the epigenetic contribution to this process. We observed an increased number of aberrantly methylated alleles in bH19, bSNRPN and bDNMT3 Lo of oocytes from small antral follicles (≤2 mm), correlating with lower developmental competence. Furthermore, we detected an increased frequency of CpG sites with an unclear methylation status for DNMT3 Ls, specifically in oocytes from follicles ≥6 mm, predominantly at three CpG positions (CpG2, CpG7 and CpG8), of which CpG7 is a potential regulatory site. No major differences in mRNA expression were observed, indicating that the transcriptional machinery may not yet be active during the follicular growth phase. Our results support the notion that a follicle diameter of ~2 mm is a critical stage for establishing DNA methylation profiles and indicate a link between DNA methylation and the acquisition of oocyte developmental competence.

scholarly journals DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors

Reproduction ◽  
2012 ◽  
Vol 144 (3) ◽  
pp. 319-330 ◽  
Author(s):  
Mike Diederich ◽  
Tamara Hansmann ◽  
Julia Heinzmann ◽  
Brigitte Barg-Kues ◽  
Doris Herrmann ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mrna Expression ◽  
Bos Taurus ◽  
Expression Profiles ◽  
Methylation Status ◽  
Transcript Abundance ◽  
Developmental Competence ◽  
Satellite Sequences ◽  
Bovine Oocytes

The developmental capacity of oocytes from prepubertal cattle is reduced compared with their adult counterparts, and epigenetic mechanisms are thought to be involved herein. Here, we analyzed DNA methylation in three developmentally important, nonimprinted genes (SLC2A1, PRDX1, ZAR1) and two satellite sequences, i.e. ‘bovine testis satellite I’ (BTS) and ‘Bos taurus alpha satellite I’ (BTαS). In parallel, mRNA expression of the genes was determined by quantitative real-time PCR. Oocytes were retrieved from prepubertal calves and adult cows twice per week over a 3-week period by ultrasound-guided follicular aspiration after treatment with FSH and/or IGF1. Both immature and in vitro matured prepubertal and adult oocytes showed a distinct hypomethylation profile of the three genes without differences between the two types of donors. The methylation status of the BTS sequence changed according to the age and treatment while the methylation status of BTαS sequence remained largely unchanged across the different age and treatment groups. Relative transcript abundance of the selected genes was significantly different in immature and in vitro matured oocytes; only minor changes related to origin and treatment were observed. In conclusion, methylation levels of the investigated satellite sequences were high (>50%) in all groups and showed significant variation depending on the age, treatment, or in vitro maturation. To what extent this is involved in the acquisition of developmental competence of bovine oocytes needs further study.

Oocyte quality and embryo production in cattle

1998 ◽  
Vol 78 (4) ◽  
pp. 513-516 ◽  
Author(s):  
M.-A. Sirard ◽  
P. Blondin
Keyword(s):  
Growth Phase ◽  
Ovarian Follicle ◽  
Oocyte Quality ◽  
Developmental Competence ◽  
Full Potential ◽  
Plateau Phase ◽  
Follicular Growth ◽  
Transvaginal Aspiration ◽  
New Evidence ◽  
Bovine Oocytes

New evidence indicates that bovine oocytes are not always in synchrony with their surrounding follicles. During rapid follicular growth, the oocytes do not acquire a full developmental competence. The plateau phase at the end of the growth phase resulting in dominance or atresia seems important for oocytes to accumulate the information required to become viable embryos. The ovarian follicle has a behavior similar to a fruit where the seed must await the ripening of the envelope to achieve its full potential. This knowledge can now be applied to harvest oocytes of higher quality through transvaginal aspiration in cows. Key words: Oocyte, embryo, cattle

Hepatic IGF1 DNA methylation is influenced by gender but not by intrauterine growth restriction in the young lamb

2015 ◽  
Vol 6 (6) ◽  
pp. 558-572 ◽  
Author(s):  
D. J. Carr ◽  
J. S. Milne ◽  
R. P. Aitken ◽  
C. L. Adam ◽  
J. M. Wallace
Keyword(s):  
Dna Methylation ◽  
Growth Hormone ◽  
Sexual Dimorphism ◽  
Mrna Expression ◽  
Intrauterine Growth Restriction ◽  
Messenger Rna ◽  
Methylation Status ◽  
Growth Restriction ◽  
Intrauterine Growth ◽  
Increased Risk

Intrauterine growth restriction (IUGR) and postnatal catch-up growth confer an increased risk of adult-onset disease. Overnourishment of adolescent ewes generates IUGR in ∼50% of lambs, which subsequently exhibit increased fractional growth rates. We investigated putative epigenetic changes underlying this early postnatal phenotype by quantifying gene-specific methylation at cytosine:guanine (CpG) dinucleotides. Hepatic DNA/RNA was extracted from IUGR [eight male (M)/nine female (F)] and normal birth weight (12 M/9 F) lambs. Polymerase chain reaction was performed using primers targeting CpG islands in 10 genes: insulin, growth hormone, insulin-like growth factor (IGF)1, IGF2, H19, insulin receptor, growth hormone receptor, IGF receptors 1 and 2, and the glucocorticoid receptor. Using pyrosequencing, methylation status was determined by quantifying cytosine:thymine ratios at 57 CpG sites. Messenger RNA (mRNA) expression of IGF system genes and plasma IGF1/insulin were determined. DNA methylation was independent of IUGR status but sexual dimorphism in IGF1 methylation was evident (M<F, P=0.008). IGF1 mRNA:18S and plasma IGF1 were M>F (both P<0.001). IGF1 mRNA expression correlated negatively with IGF1 methylation (r=−0.507, P=0.002) and positively with plasma IGF1 (r=0.884, P<0.001). Carcass and empty body weights were greater in males (P=0.002–0.014) and this gender difference in early body conformation was mirrored by sexual dimorphism in hepatic IGF1 DNA methylation, mRNA expression and plasma IGF1 concentrations.

scholarly journals Bisphenol a Exposure, DNA Methylation, and Asthma in Children

2020 ◽  
Vol 17 (1) ◽  
pp. 298 ◽  
Author(s):  
Chia-Feng Yang ◽  
Wilfried J. J. Karmaus ◽  
Chen-Chang Yang ◽  
Mei-Lien Chen ◽  
I-Jen Wang
Keyword(s):  
Dna Methylation ◽  
Bisphenol A ◽  
Candidate Genes ◽  
Promoter Methylation ◽  
Childhood Asthma ◽  
Methylation Status ◽  
Differential Methylation ◽  
Study Cohort ◽  
Blood Samples ◽  
Asthma In Children

Epidemiological studies have reported the relationship between bisphenol A (BPA) exposure and increased prevalence of asthma, but the mechanisms remain unclear. Here, we investigated whether BPA exposure and DNA methylation related to asthma in children. We collected urinary and blood samples from 228 children (Childhood Environment and Allergic Diseases Study cohort) aged 3 years. Thirty-three candidate genes potentially interacting with BPA exposure were selected from a toxicogenomics database. DNA methylation was measured in 22 blood samples with top-high and bottom-low exposures of BPA. Candidate genes with differential methylation levels were validated by qPCR and promoter associated CpG islands have been investigated. Correlations between the methylation percentage and BPA exposure and asthma were analyzed. According to our findings, MAPK1 showed differential methylation and was further investigated in 228 children. Adjusting for confounders, urinary BPA glucuronide (BPAG) level inversely correlated with MAPK1 promoter methylation (β = −0.539, p = 0.010). For the logistic regression analysis, MAPK1 methylation status was dichotomized into higher methylated and lower methylated groups with cut off continuous variable of median of promoter methylation percentage (50%) while performing the analysis. MAPK1 methylation was lower in children with asthma than in children without asthma (mean ± SD; 69.82 ± 5.88% vs. 79.82 ± 5.56%) (p = 0.001). Mediation analysis suggested that MAPK1 methylation acts as a mediation variable between BPA exposure and asthma. The mechanism of BPA exposure on childhood asthma might, therefore, be through the alteration of MAPK1 methylation. The mechanism of BPA exposure on childhood asthma might, therefore, be through the alteration of MAPK1 methylation.

Effect of increased oxygen availability and astaxanthin supplementation on the growth, maturation and developmental competence of bovine oocytes derived from early antral follicles

2020 ◽  
Vol 157 ◽  
pp. 341-349
Author(s):  
Madalitso Chelenga ◽  
Kenichiro Sakaguchi ◽  
Mohammed A. Abdel-Ghani ◽  
Yojiro Yanagawa ◽  
Seiji Katagiri ◽  
...  
Keyword(s):  
Oxygen Availability ◽  
Developmental Competence ◽  
Antral Follicles ◽  
Bovine Oocytes

scholarly journals Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos

2016 ◽  
Vol 62 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Yan Jun HUAN ◽  
Zhan Feng WU ◽  
Ji Guang ZHANG ◽  
Jiang ZHU ◽  
Bing Teng XIE ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Status ◽  
Developmental Competence ◽  
Donor Cells

scholarly journals Flavonoid quercetin modulates glucose metabolism by up-regulating the methylation status of genes as Kcnj11, Gys1 and Erp29 in liver of Wistar rats

2020 ◽  
Author(s):  
Weina Gao ◽  
Xiangyu Bian ◽  
Yuying Ma ◽  
Yijing Yu ◽  
Tala Shi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Endoplasmic Reticulum ◽  
Glucose Metabolism ◽  
Mrna Expression ◽  
Quantitative Pcr ◽  
Pathway Analysis ◽  
Promoter Methylation ◽  
Wistar Rats ◽  
Methylation Status ◽  
Protein Processing

Abstract Background: Quercetin is a flavonoid that exists extensively in vegetables and fruits, and has many biological activities. It is reported that quercetin participates in the regulation of glucose metabolism through various mechanisms. However, whether DNA methylation is involved in those regulatory effects remains unclear. As liver is one of the main organ involved in methyl and glucose metabolism, DNA methylation targets related to glucose metabolism were identified in liver of Wistar rats upon quercetin exposure. Methods: The rats were fed a control diet or a 0.5% quercetin-supplemented diet for 6 weeks. Arraystar Rat 4 × 180K RN4 RefSeq Promoter Arrays were used for a genome-wide survey of DNA methylation in rat livers. NimbleScan v2.5 software was used to process microarray data. DAVID software was used to perform GO and Pathway analysis of regulatory networks. Gene promoter methylation status was examined by the ChIP-quantitative PCR assay, and hepatic levels of methylated Kcnj11, Erp29, Gys1, Flot1 and GAPDH were evaluated. Gene expression was assayed by quantitative PCR, and hepatic mRNA expression levels of Kcnj11, Gys1 and Erp29 were estimated. Results: Quercetin induced specific changes in DNA methylation. A total of 1,263 differentially expressed genes were found in 22 chromosomes, particularly on chromosomes 1, 3, 5, 7, 8, and 10. According to GO functional analysis, differential genes have focused on organic substance, cellular and primary metabolic process. According to pathway analysis, the most enrichment pathways included Type 2 diabetes mellitus, insulin signaling pathway and protein processing in endoplasmic reticulum. Nineteen up-methylated genes were found among several biological pathways after quercetin treatment. Critical genes and pathways associated with glucose metabolism (Kcnj11 and Gys1) and protein processing in the endoplasmic reticulum (Erp29) were changed significantly. Promoter methylation levels of Kcnj11, Gys1, and Erp29 were significantly increased, and the mRNA expression of those genes significantly decreased simultaneously upon quercetin exposure. Conclusions: Quercetin changed the promoter methylation status and expression of Kcnj11, Gys1, and Erp29, which are mainly related to glucose metabolism. The gene Kcnj11, Gys1, and Erp29 could be novel epigenetic targets of quercetin in regulating glucose metabolism.

scholarly journals Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma.

2021 ◽  
Author(s):  
Huaxiang Wang ◽  
Fengfeng Feng Xu ◽  
Lizhi Lv ◽  
Ruling Wang ◽  
Bin Jiang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Mrna Expression ◽  
Prognostic Value ◽  
Methylation Status ◽  
Alpha Fetoprotein ◽  
Gene Set Enrichment Analysis ◽  
Cox Regression Analysis ◽  
Tumor Stages ◽  
Diagnostic And Prognostic Value

Abstract Background Chaperonin containing TCP1 subunit 7 (CCT7), a member of the chaperonin containing TCP1 complex (CCT), has been reported regulating the expression of many tumor-related proteins. In this study, we investigated the diagnostic and prognostic value of CCT7 expression for hepatocellular carcinoma (HCC). Methods We investigated the CCT7 expression in HCC in The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and our cohort. The diagnostic and prognostic value were verified by receiver operating characteristic curve (ROC) analysis and Kaplan-Meier analysis, respectively. The association between CCT7 expression with DNA methylation status was investigated in the TCGA database. Gene ontology (GO), The Kyoto Encyclopedia of Genes and Genomes (KEGG), and gene set enrichment analysis (GSEA) analysis were employed to identify the potential pathway in which CCT7 is involved in tumorigenesis and progression. Results CCT7 expression in HCC was significantly higher than adjacent normal tissues, and elevated CCT7 expression correlated with tumor stages and tumor grade. Furthermore, the ROC curve showed CCT mRNA expression has a better diagnostic value for HCC with early-stage and low alpha-fetoprotein expression. Positive predictive value (PPV) of CCT7 was higher than alpha-fetoprotein both in the GEO and TCGA database. The Multivariate Cox Regression analysis of clinicopathologic characteristics revealed that both high mRNA and protein expression of CCT7 were independent risk factors for overall survival (OS) and recurrence-free survival (RFS). High DNA methylation of CpG site(cg19515186) was associated with low CCT7 expression and better OS in HCC. The GO, KEGG and GSEA analysis demonstrated that CCT7 mRNA expression was associated with Spliceosome signaling pathway. Conclusions The findings of this study demonstrated that CCT7 has diagnostic and prognostic value for HCC.

Sign in / Sign up

Export Citation Format

Share Document