91 Can propylene glycol modulate insulin and insulin-like growth factor-1 in superovulated dairy heifers?

2019 ◽  
Vol 31 (1) ◽  
pp. 171
Author(s):  
R. Dupras ◽  
L. Mills ◽  
G. Robert ◽  
C. Meunier ◽  
Y. Chorfi
Keyword(s):  
Growth Factor ◽  
Propylene Glycol ◽  
Serum Insulin ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Serum Concentrations ◽  
Dairy Heifers ◽  
Glycol Solution ◽  
Post Hoc ◽  
And Control

The aim of this study was to determine the effect of propylene glycol (PPG) on serum concentrations of insulin and insulin-like growth factor (IGF)-1 in superovulated dairy heifers. We hypothesised that administration of PPG would have a positive effect on superovulation results via increased insulin and IGF-1. A total of 20 clinically healthy Holstein heifers with an average age of 12 months were used for this experiment. Superovulation was performed using a standard protocol. Briefly, each heifer received 3mg of oestradiol-17β IM and an intravaginal progesterone-releasing insert (CIDR) containing 1.9g of progesterone at random stages of the oestrous cycle (designated Day 0). From Day 4 to 8, heifers received a total of 200mg of NIH-follicle-stimulating hormone-P1 administered intramuscularly through 9 injections of decreasing doses (from 50 to 10mg) at 12-h intervals. On Day 7, heifers received 2 injections of 500µg of cloprostenol, a PGF2α analogue, at ~6-h intervals, after which intravaginal inserts were removed. Artificial insemination was performed on Day 10, 12h after treatment with 100µg of gonadotropin-releasing hormone IM. Embryos were flushed from the uterus of donor heifers 6 days after AI. The method consisted of simultaneously using 1 catheter per uterine horn. Catheters were maintained in place to perform 2 flushes 1h apart. A total of 1L of flushing medium was used, 700 and 300mL for the first and the second flush, respectively. Embryos were assessed for viability immediately after collection using the IETS classification. Heifers were divided into 2 groups (PPG and control group). From Day 4 to 14 of the superovulation protocol, PPG group received a daily dose of 400mL of a 66.7% propylene glycol solution, whereas the control group received the same amount of water. Two months later, the same experiment was conducted by inverting the groups. At Day 4 and 14, four blood samples were collected to measure insulin and IGF-1 at 25-min intervals. The first sample (0) was taken before heifers received PPG or water. Insulin was analysed using an ELISA kit following manufacturer’s instructions, whereas IGF-1 was determined using a chemiluminescence immunoassay. Embryo associated data were analysed using t-test. Both IGF-1 and insulin data were analysed using a two-way ANOVA, followed by Bonferroni post-hoc test. Treatment with PPG had no effect on the number of transferable embryos (8±5.1v. 7±5.5), degenerated embryos (0.5±0.8v. 1.5±2.4), or unfertilized oocytes (0.3±0.7v. 0.7±1.2) recovered. There was also no effect of PPG on IGF-1 serum concentrations at the beginning (Day 4) or the end (Day 14) of the treatment regimen. However, PPG treatment enhanced (P = 0.02) serum insulin concentrations 50min after administration on Day 4 (10.69 v. 6.88 pmol/L), as well as at 25 (19.58 v. 9.64 pmol/L) and 50min (16.67v. 8.21 pmol/L) on Day 14. It has been suggested that PPG metabolism may stimulate insulin and IGF-1 secretion, which can promote embryo development. However, in the present study, there was no effect of oral doses of PPG on IGF-1. Although higher serum concentrations of insulin were observed after PPG treatment, there was no effect of PPG treatment on the number of transferable embryos recovered following superovulation.

The effects of adenoidectomy of serum insulin-like growth factor-1 (IGF-1) and ghrelin in hypertrophied adenoids in children with otitis media with effusion

2020 ◽  
Vol 74 (4) ◽  
pp. 13-17
Author(s):  
Beata Żelazowska-Rutkowska ◽  
Klaudia Jacewicz ◽  
Edwina Kasprzycka ◽  
Bożena Skotnicka ◽  
Bogdan Cylwik
Keyword(s):  
Growth Factor ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Reference Group ◽  
Serum Insulin ◽  
Serum Levels ◽  
Control Group ◽  
Healthy Children ◽  
Insulin Like Growth Factor ◽  
Significant Difference

<b>Aim:</b> The aim of the current study was to assess the serum levels of insulin-like growth factor-1 (IGF-1) and ghrelin in hypertrophied adenoids in children suffering with or without otitis media with effusion before and after adenoidectomy. <br><b>Material and methods:</b> Serum IGF-1 and ghrelin concentrations were measured with specific enzyme-linked immunoassay (ELISA) methods. The study was carried out in 20 children with otitis media with effusion. The reference group comprised 24 children with hypertrophied adenoid, while control group included 19 children. <br><b>Results:</b> This mean values of IGF-1 in children with otitis media with effusion and children with hypertrophied adenoid before adenoidectomy were significantly lower than those found in healthy children. Serum levels of IGF-1 were higher after adenoidectomy. There was a significant difference of serum ghrelin levels between both examined groups and the control group. <br><b>Conclusion:</b> Our results suggest that adenoidectomy in children with hypertrophied adenoids and in children with otitis media with effusion significantly increases the level of IGF-1 in serum compared to before surgery through the effect of the GH-IGF-1 axis, which could contribute to children’s growth.

scholarly journals Inflammation is a modulator of the insulin-like growth factor (IGF)/IGF-binding protein system inducing reduced bioactivity of IGFs in cystic fibrosis

2006 ◽  
Vol 154 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Maria E Street ◽  
Maria A Ziveri ◽  
Cinzia Spaggiari ◽  
Isabella Viani ◽  
Cecilia Volta ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Growth Factor ◽  
Binding Protein ◽  
Serum Insulin ◽  
Molecular Forms ◽  
Insulin Like Growth Factor ◽  
Serum Concentrations ◽  
C Peptide ◽  
Igf I ◽  
Igfbp 3

Objective: In inflammatory bowel diseases, increased serum interleukin (IL)-6 levels are associated with high serum insulin-like growth factor-binding protein 2 (IGFBP-2) levels, and cytokines modify the insulin-like growth factor (IGF)/IGFBP system in models in vitro. In cystic fibrosis (CF) the IGF/IGFBP system has not been extensively studied, and relationships with proinflammatory cytokines have not been explored. The aim of this study was to investigate the IGF/IGFBP system and verify changes dependent on IL-1β, IL-6, tumour necrosis factor α (TNFα), and insulin. Methods: Eighteen subjects with CF (mean age 26.6 ± 1.1 years) and 18 controls, comparable for age, sex, and body mass index, were enrolled. Serum IGF-I, IGF-II, IGFBP-2, IGFBP-3, IL-1β, IL-6, TNFα, insulin and C-peptide were measured. Different molecular forms of IGFBP-2 and IGFBP-3 were investigated by Western immunoblotting. The patients were analysed as a whole and as two subgroups depending on established clinical criteria (Swachman–Kulczycki score). Results: Patients had higher serum concentrations of IL-1β, IL-6, TNFα and IGFBP-2 than controls. Serum concentrations of IGF-I and IGF-II were significantly lower and insulin and C-peptide levels significantly increased in CF compared with healthy controls whereas IGFBP-3 serum concentrations were similar, with comparable IGF-I/IGFBP-3 and decreased IGF-I/IGFBP-2 and IGF-II/IGFBP-2 molar ratios. From correlation analysis we detected a significant positive correlation between IGFBP-2 and IL-6 and a negative correlation between IGFBP-2 and IGFBP-3. Conclusions: Our findings suggest that inflammation is an important modulator of the IGF/IGFBP system with an overall reduction in IGF bioactivity in CF.

Effect of propylene glycol supplementation around parturition on milk yield, reproduction performance and some hormonal and metabolic characteristics in dairy cows

1996 ◽  
Vol 63 (1) ◽  
pp. 11-24 ◽  
Author(s):  
Andrea Formigoni ◽  
Marie-Christine Cornil ◽  
Alberto Prandi ◽  
Archimede Mordenti ◽  
Andriana Rossi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Propylene Glycol ◽  
Milk Yield ◽  
Post Partum ◽  
Treated Group ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Metabolic Characteristics ◽  
Cholesterol Levels ◽  
Milk Urea

SummaryThirty-nine multiparous Holstein cows were used to measure the effect of propylene glycol treatment around parturition on milk yield, reproductive efficiency and some hormone and metabolite concentrations. Cows were assigned randomly to control (n = 19) or propylene glycol treated (n = 20) groups. Propylene glycol (300g) was administered directly mixed with the diet from day 10 prior to the expected calving date until parturition (day 0) and orally after dilution in 1 l water on days 3, 6, 9 and 12. Blood samples were collected on days −20, −5, 0, 3, 10, 25 and 50 while milk samples were taken weekly until 13 weeks post partum. Body condition scores, recorded on days −20, 15 and 50, were not affected by propylene glycol administration. Propylene glycol did not significantly affect milk yield or composition but linear somatic cell score measured from the first 13 weeks post partum was reduced by propylene glycol administration (P < 0·01). Moreover, propylene glycol reduced milk urea (−25 mg/l, P < 0·05), especially during the first 9 weeks post partum. Plasma insulin concentrations were similar in both groups during the experiment while insulin-like growth factor I (P < 0·05) and insulin-like growth factor-binding protein 3 (P < 0·001) levels were higher on days 10, 25 and 50 post partum in the propylene glycol group. Propylene glycol administration decreased plasma non-esterified fatty acid concentrations (P < 0·05 to P < 0·01) but increased total cholesterol levels (P < 0·01) after parturition while 3-hydroxy-butyrate levels were unaffected by the treatment. Changes in the hormone and metabolic concentrations after propylene glycol administration in the last few days of gestation and the first week of lactation seem to indicate that energy balance in the treated group was probably more positive than in the control group. There was also evidence that propylene glycol administration prevented fatty liver syndrome and hastened the resumption of oestrous cycles (P < 0·001).

scholarly journals The role of insulin-like growth factor in Acrochordon Etiopathology

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Hanife Gündoğdu Köseoğlu ◽  
Burçin Cansu Bozca ◽  
Cumhur İbrahim Başsorgun ◽  
Ramazan Sarı ◽  
Sadıka Halide Akbaş ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Growth Factor ◽  
Serum Insulin ◽  
Tissue Expression ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Insulin Metabolism ◽  
Skin Tag ◽  
Igf Receptors ◽  
History Of

Abstract Background There are reports that acrochordon (skin tag), the most common fibroepithelial tumor of the skin, may be associated with metabolic syndrome components, particularly insulin metabolism disorders. However, to the best of our knowledge, there is no study examining its association with insulin resistance and tissue levels of insulin-like growth factor 1 receptor (IGF-1R) and insulin-like growth factor 2 receptor (IGF-2R). Methods Thirty patients with at least one acrochordon in their body who had no known history of diabetes mellitus and a control group comprised 30 individuals who had no acrochordon or no known history of diabetes mellitus were included. The tissue expression of IGF-1R and IGF-2R were investigated via immunohistochemical assessment in both groups. Results In the group with acrochordon, IGF-1R and IGF-2R expression was found to be significantly higher compared to the control group (p < 0,01). Using logistic regression analysis, an increase in serum insulin, serum IGF-1 and HOMA-IR levels was found to be associated with the expression levels of IGF-1R and IGF-2R. Conclusion These findings support the view that insulin metabolism disorders should be evaluated in patients with acrochordon. Our study indicates that IGF receptors may have an effect on acrochordon pathogenesis and that acrochordon etiology and related conditions can be clarified by detection of parameters that influence receptor levels.

scholarly journals The Role of Insulin-Like Growth Factor in the Acrochordon Etiopathology

2019 ◽  
Author(s):  
Hanife Gündoğdu Köseoğlu ◽  
Burçin Cansu Bozca ◽  
Cumhur Ibrahım Bassorgun ◽  
Ramazan Sarı ◽  
Sadıka Halide Akbas ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Growth Factor ◽  
Serum Insulin ◽  
Tissue Level ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Insulin Metabolism ◽  
Tissue Samples ◽  
Skin Tag ◽  
History Of

Abstract Background There are reports that acrochordons (skin tag), the most common fibroepithelial tumor of the skin, may be associated with metabolic syndrome components, particularly insulin metabolism disorders. However, to the best of our knowledge, there is no study examining its association with the insulin resistance, insulin-like growth factor 1 receptor (IGF-1R) and insulin-like growth factor 2 receptor (IGF-2R) levels at tissue level yetMethods Thirty patients with at least 1 acrochordon in their body who had no known history of diabetes mellitus and the control group comprised 30 individuals who had no acrochordon or no known history of diabetes mellitus were included. IGF-1R and IGF-2R expression was investigated using immunohistochemical assessment administered to the tissue samples from the study and control groups.Results In the group with acrochordon, IGF-1R and IGF-2R expression was found to be significantly higher compared to the control group (p<0,01). IGF-1R expression was found to correlate with serum IGF-1 and BMI (p=0,03 r=0,028 and p=0,048 r=0,257 respectively). IGF-2R expression was found to correlate with BMI and HOMA-IR (p=0,03 r=0,357 and p=0,046 r=0,256 respectively). Using logistic regression analysis, an increase in serum insulin, serum IGF-1 and HOMA-IR levels was shown to increase IGF-1R and IGF-2R expression rates.Conclusion These findings support the view that insulin metabolism disorders in patients with acrochordon should be evaluated. Our study indicates that insulin like growth factor receptors may have an effect on the acrochordon pathogenesis and that acrochordon etiology and related conditions can be clarified by detection of parameters that influence the receptor levels.

Insulin-like growth factor and growth hormone receptor in nephrotic rats

1994 ◽  
Vol 266 (1) ◽  
pp. E102-E106
Author(s):  
M. A. Thabet ◽  
A. Challa ◽  
W. Chan ◽  
F. Liu ◽  
R. L. Hintz ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Nephrotic Syndrome ◽  
Growth Factor ◽  
Growth Retardation ◽  
Hormone Receptor ◽  
Growth Hormone Receptor ◽  
Serum Insulin ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Igf I

In an attempt to elucidate the mechanism of growth retardation in the nephrotic syndrome, specific serum and hepatic growth factors were measured in Sprague-Dawley rats in which nephrotic syndrome was produced by administration of puromycin (1.5 mg.100 g body wt-1.day-1) for 12 days. On the 13th day, the results of these nephrotic animals were compared with those of an equal number of pair-fed and control animals: the mean dietary intake of the nephrotic group was 71% that of the control group (P < 0.001). Serum insulin-like growth factor (IGF) binding protein-3 was significantly reduced (P < 0.005) in the nephrotic rats, compared with the pair-fed and the control groups. Hepatic IGF-I mRNA in the nephrotic rats averaged 36% that of control (P < 0.001) and 46% that of the pair-fed animals (P < 0.001). Hepatic growth hormone receptor (GHr) mRNA in the nephrotic rats averaged 19% of that of the control (P < 0.001) and 27% of that of the pair-fed rats (P < 0.001). These results indicate that the growth retardation of the nephrotic rats may be associated with the significant decrease in IGF-I mRNA and reduction in GHr mRNA.

scholarly journals Vitamin D improves the content of TGF-β and IGF-1 in intervertebral disc of diabetic rats

2017 ◽  
Vol 242 (12) ◽  
pp. 1254-1261 ◽  
Author(s):  
Ji-long An ◽  
Wei Zhang ◽  
Jian Zhang ◽  
Li-chao Lian ◽  
Yong Shen ◽  
...  
Keyword(s):  
Vitamin D ◽  
Growth Factor ◽  
Intervertebral Disc ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Normal Group ◽  
Degenerative Changes ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
And Control

Objective: The contents of transforming growth factor-β and insulin-like growth factor-1 in disc of diabetic rats were measured at three different periods after injected with 1,25-Dihydroxyvitamin D3, and compared with that in normal rats. The significance of content changes was also discussed. Methods: Fourty-five Sprague-Dawley (SD) rats were divided into three groups, namely the experimental group (STZ+calcitriol), control group (STZ+citrate buffer), and normal group (citrate buffer). Complete lumbar discs in these groups were obtained at the second, fourth, sixth week, respectively. After paraffin-embedded sections and HE staining, the structure and morphology changes of disc were observed. The content of transforming growth factor-β and insulin-like growth factor-1 was measured by immunohistochemical method, and the expression of transforming growth factor-β and insulin-like growth factor-1 was detected by Western Blot. Results: In hematoxylin–eosin staining, degenerative changes were observed in disc of experimental and control group at three different periods, and there were no changes in disc in normal group. Immunohistochemical method indicated the content of transforming growth factor-β and insulin-like growth factor-1 in experimental and control group was significantly lower than normal group at three different periods ( P < 0.05). And there were significant differences between experimental and control group at three different periods ( P < 0.05). Conclusion: Vitamin D can protect the degeneration of intervertebral disc and improve the content of transforming growth factor-β and insulin-like growth factor-1 in the intervertebral disc, which provides a new idea for the prevention and treatment of degenerative changes of the intervertebral disc in diabetic patients. Impact statement No researchers reported Vitamin D could protect degeneration of intervertebral disc. That is to say, we found a new method to prevent and treat degenerative changes of the intervertebral disc in diabetic patients. And Vitamin D prevented the discs by improving the content of TGF-β and IGF-1.

scholarly journals The Role of Insulin-like Growth Factor in Acrochordon Etiopathology

2020 ◽  
Author(s):  
Hanife Gündoğdu Köseoğlu ◽  
Burçin Cansu Bozca ◽  
Cumhur Ibrahım Bassorgun ◽  
Ramazan Sarı ◽  
Sadıka Halide Akbas ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Growth Factor ◽  
Serum Insulin ◽  
Tissue Expression ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Insulin Metabolism ◽  
Skin Tag ◽  
Igf Receptors ◽  
History Of

Abstract Background: There are reports that acrochordon (skin tag), the most common fibroepithelial tumor of the skin, may be associated with metabolic syndrome components, particularly insulin metabolism disorders. However, to the best of our knowledge, there is no study examining its association with insulin resistance and tissue levels of insulin-like growth factor 1 receptor (IGF-1R) and insulin-like growth factor 2 receptor (IGF-2R).Methods: Thirty patients with at least one acrochordon in their body who had no known history of diabetes mellitus and a control group comprised 30 individuals who had no acrochordon or no known history of diabetes mellitus were included. The tissue expression of IGF-1R and IGF-2R were investigated via immunohistochemical assessment in both groups.Results: In the group with acrochordon, IGF-1R and IGF-2R expression was found to be significantly higher compared to the control group (p<0,01). Using logistic regression analysis, an increase in serum insulin, serum IGF-1 and HOMA-IR levels was found to be associated with the expression levels of IGF-1R and IGF-2R.Conclusion: These findings support the view that insulin metabolism disorders should be evaluated in patients with acrochordon. Our study indicates that IGF receptors may have an effect on acrochordon pathogenesis and that acrochordon etiology and related conditions can be clarified by detection of parameters that influence receptor levels.

scholarly journals 284THE EFFECT OF FEEDING PROPYLENE GLYCOL TO DAIRY COWS DURING THE EARLY POSTPARTUM PERIOD ON SERUM INSULIN CONCENTRATION AND THE RELATIONSHIP WITH OOCYTE DEVELOPMENTAL COMPETENCE

2004 ◽  
Vol 16 (2) ◽  
pp. 262
Author(s):  
D. Rizos ◽  
W. Griffin ◽  
P. Duffy ◽  
M. Wade ◽  
K. Quinn ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Propylene Glycol ◽  
Serum Insulin ◽  
Developmental Competence ◽  
Insulin Concentration ◽  
Control Group ◽  
Serum Insulin Concentration ◽  
Oocyte Developmental Competence ◽  
And Control

High yielding dairy cows are typically in negative energy balance postpartum (pp). It has been shown that initiation of the first pp ovulation and, therefore, the resumption of normal oestrous cycles is delayed in high genetic merit dairy cows and is associated with lower circulating insulin concentration (Gutierrez et al., 1999 J. Reprod. Fertil. 24, 32 abst). Evidence shows that propylene glycol (PG) rapidly elevates systemic concentrations of insulin (Bremmer et al., 2000 J. Dairy Sci. 83, 2239–2251). The aim of this study was to determine the effects of PG feeding to dairy cows in the early pp period, on serum insulin and ovarian function, and on oocyte developmental competence after in vitro maturation, fertilization, and culture. Thirteen Holstein-Friesian cows were assigned to PG (n=6) or control (n=7) groups. Each treated cow received 500mL of PG and each control was given 500mL of water daily from Day 5pp until day of AI. Blood samples for insulin were collected at 0, 30, 60 and 90min post-drenching on Days 5, 15 and 25 pp. All cows were fed 3kg concentrates at milking (twice daily) and had ad libitum access to a 50:50 maize silage : grass silage forage from the time of last blood collection. Oocytes were collected by ovum pick-up (OPU) in four sessions (following treatment with pFSH) beginning on Day 25–35 pp. The recovered oocytes were graded (Grade 1 to 4) in terms of their surrounding cumulus cells and the appearance of the cytoplasm. Grade 1–2 oocytes were matured in vitro, then fertilized using frozen-thawed bull semen, and subsequently cultured up to Day 8 in synthetic oviduct fluid. All data were analyzed using SAS version 6.12 and split-plot designs, following square root or arc sine transformation, if appropriate. PG significantly increased (P&lt;0.001) serum insulin concentration (0min: 1.55±0.19; 30min: 4.48±0.82; 60min: 4.74±0.72; 90min: 4.10±0.56) compared to the control group (0min: 1.91±0.28; 30min 1.96±0.27; 60min: 2.37±0.44; 90min: 2.04±0.26). The follicle size distribution was similar between treated and control cows for categories 2–4mm (4.0±0.47; 4.3±0.70), 8–10mm (3.2±0.47; 2.5±0.39), and &gt;10mm (0.42±0.12; 0.67±0.17). However, there were significantly more follicles in the 5–7mm category (6.2±0.82 v. 3.3±0.43; P&lt;0.05) for treated cows. The number of follicles punctured (13.8±1.02; 10.7±1.04), the number of oocytes recovered (4.5±0.53; 3.5±0.61), and the number of Grade 1–2 oocytes (2.8±0.35; 1.8±0.35) were not different between treated and control cows. Although cleavage rate (68.3 v. 58.9%) and blastocyst yield (25.3 v. 14.4%) were higher for treated cows, the differences were not significant. In conclusion, these results indicate that feeding cows with PG during the early pp period increased the circulating insulin concentration. However, the developmental competence of the recovered oocytes did not differ between the groups.

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