511. THE EFFECTS OF HIGH FAT DIET ON LIPID LOCALISATION IN THE PERI-OVULATORY CUMULUS OOCYTE COMPLEX

2009 ◽  
Vol 21 (9) ◽  
pp. 110
Author(s):  
X. Yang ◽  
K. R. Dunning ◽  
T. E. Hickey ◽  
R. J. Norman ◽  
X. Liang ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Fat Diet ◽  
Neutral Lipids ◽  
Control Diet ◽  
Cumulus Cells ◽  
Early Embryo ◽  
Developmental Competence ◽  
Obese Women ◽  
High Fat ◽  
Cumulus Oocyte Complex

Intracellular neutral lipids are stored in discrete droplets that are surrounded by lipid associated proteins, such as adipophilin and perilipin, which control cellular lipid metabolism by regulating the access of lipases. The role of lipids in oocyte maturation is unclear, although they have a potential role as an energy source for the oocyte and early embryo. To elucidate potential mechanisms controlling lipid utilisation in the peri-ovulatory cumulus-oocyte-complex (COC) we 1) localised lipid droplets by immunohistochemistry for adipophilin and perilipin and direct staining of neutral lipids with BODIPY and 2) investigated whether a high fat diet can alter oocyte lipid quantity or localisation. Ovaries were isolated from 21 day old mice before and 10h after the ovulation stimulus hCG. Adipophilin and perilipin were both detected by immunohistochemistry in peri-ovulatory follicles with similar localisation before and after hCG. In separate experiments, adult mice were fed a high fat or control diet for 4 weeks and COCs were isolated from preovulatory follicles prior to hCG or from the oviduct 13h after hCG stimulation followed by BODIPY staining and quantification with confocal microscopy. BODIPY staining showed that COCs possess low levels of lipids evenly distributed in the oocyte before hCG but increased lipid assembled as droplets in the oocyte after ovulation. In mice fed a high fat diet, intracellular lipids were markedly increased in both the cumulus cells and oocytes from preovulatory and ovulated COCs. The ubiquitous expression of lipid droplet proteins in the peri-ovulatory follicle together with the changes in neutral lipid storage concurrent with ovulation suggests that lipid metabolism play an important role in oocyte release, transport and/or developmental competence. Furthermore, the dramatic effect of dietary fat on COC lipid content may contribute to the impaired oocyte quality we have observed in obese mice as well as reduced fertility in obese women

scholarly journals Flavonoids from Mulberry Leaves Alleviate Lipid Dysmetabolism in High Fat Diet-Fed Mice: Involvement of Gut Microbiota

2020 ◽  
Vol 8 (6) ◽  
pp. 860 ◽  
Author(s):  
Yinzhao Zhong ◽  
Bo Song ◽  
Changbing Zheng ◽  
Shiyu Zhang ◽  
Zhaoming Yan ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Lipid Metabolism ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Control Diet ◽  
Liver Steatosis ◽  
High Fat ◽  
Acetic Acid Production ◽  
Mulberry Leaves ◽  
Brown Adipose

Here, we investigated the roles and mechanisms of flavonoids from mulberry leaves (FML) on lipid metabolism in high fat diet (HFD)-fed mice. ICR mice were fed either a control diet (Con) or HFD with or without FML (240 mg/kg/day) by oral gavage for six weeks. FML administration improved lipid accumulation, alleviated liver steatosis and the whitening of brown adipose tissue, and improved gut microbiota composition in HFD-fed mice. Microbiota transplantation from FML-treated mice alleviated HFD-induced lipid metabolic disorders. Moreover, FML administration restored the production of acetic acid in HFD-fed mice. Correlation analysis identified a significant correlation between the relative abundances of Bacteroidetes and the production of acetic acid, and between the production of acetic acid and the weight of selected adipose tissues. Overall, our results demonstrated that in HFD-fed mice, the lipid metabolism improvement induced by FML administration might be mediated by gut microbiota, especially Bacteroidetes-triggered acetic acid production.

scholarly journals Tis7 deletion reduces survival and induces intestinal anastomotic inflammation and obstruction in high-fat diet-fed mice with short bowel syndrome

2014 ◽  
Vol 307 (6) ◽  
pp. G642-G654 ◽  
Author(s):  
Amy M. Garcia ◽  
Derek Wakeman ◽  
Jianyun Lu ◽  
Christopher Rowley ◽  
Taylor Geisman ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Small Bowel ◽  
Short Bowel Syndrome ◽  
High Fat Diet ◽  
Adaptive Response ◽  
Bowel Resection ◽  
Control Diet ◽  
Small Bowel Resection ◽  
High Fat ◽  
Short Bowel

Effective therapies are limited for patients with parenteral nutrition-dependent short bowel syndrome. We previously showed that intestinal expression of the transcriptional coregulator tetradecanoyl phorbol acetate-induced sequence 7 ( tis7) is markedly increased during the adaptive response following massive small bowel resection and tis7 plays a role in normal gut lipid metabolism. Here, we further explore the functional implications of tis7 deletion in intestinal lipid metabolism and the adaptive response following small bowel resection. Intestinal tis7 transgenic ( tis7tg), tis7−/−, and wild-type (WT) littermates were subjected to 50% small bowel resection. Mice were fed a control or a high-saturated-fat (42% energy) diet for 21 days. Survival, body weight recovery, lipid absorption, mucosal lipid analysis, and the morphometric adaptive response were analyzed. Quantitative real-time PCR was performed to identify tis7 downstream gene targets. Postresection survival was markedly reduced in high-fat, but not control, diet-fed tis7−/− mice. Decreased survival was associated with anastomotic inflammation and intestinal obstruction postresection. High-fat, but not control, diet-fed tis7−/− mice had increased intestinal IL-6 expression. Intestinal lipid trafficking was altered in tis7−/− compared with WT mice postresection. In contrast, high-fat diet-fed tis7tg mice had improved survival postresection compared with WT littermates. High-fat diet feeding in the setting of tis7 deletion resulted in postresection anastomotic inflammation and small bowel obstruction. Tolerance of a calorie-rich, high-fat diet postresection may require tis7 and its target genes. The presence of luminal fat in the setting of tis7 deletion promotes an intestinal inflammatory response postresection.

scholarly journals Maternal Manganese Restriction Increases Susceptibility to High-Fat Diet-Induced Dyslipidemia and Altered Adipose Function in WNIN Male Rat Offspring

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Manisha Ganeshan ◽  
Pothaganti B. Sainath ◽  
Inagadapa J. Naga Padmavathi ◽  
Lagishetty Venu ◽  
Yedla Durga Kishore ◽  
...  
Keyword(s):  
Body Composition ◽  
Lipid Metabolism ◽  
High Fat Diet ◽  
Control Diet ◽  
Male Rat ◽  
Restricted Diet ◽  
High Fat ◽  
Rat Offspring ◽  
Ad Libitum ◽  
Fat Feeding

Growthin uterois largely a reflection of nutrient and oxygen supply to the foetus. We studied the effects of Mn restrictionper se, maternal Mn restriction, and postnatal high-fat feeding in modulating body composition, lipid metabolism and adipocyte function in Wistar/NIN (WNIN) rat offspring. Female weanling, WNIN rats receivedad libitumfor 4 months, a control or Mn-restricted diet and were mated with control males. Some restricted mothers were rehabilitated with control diet from conception (MnRC) or parturition (MnRP), and their offspring were raised on control diet. Some restricted offspring were weaned onto control diet (MnRW), while others continued on restricted diet throughout (MnR). A set of offspring from each group was fed high-fat diet from 9 months onwards. Body composition, adipocytes function, and lipid metabolism were monitored in male rat offspring at regular intervals. Maternal manganese restriction increased the susceptibility of the offspring to high-fat-induced adiposity, dyslipidaemia, and a proinflammatory state but did not affect their glycemic or insulin status.

scholarly journals Adverse effects of obesity and/or high-fat diet on oocyte quality and metabolism are not reversible with resumption of regular diet in mice

2015 ◽  
Vol 27 (4) ◽  
pp. 716 ◽  
Author(s):  
Kasey A. Reynolds ◽  
Anna L. Boudoures ◽  
Maggie M.-Y. Chi ◽  
Qiang Wang ◽  
Kelle H. Moley
Keyword(s):  
Adverse Effects ◽  
High Fat Diet ◽  
Control Diet ◽  
Intervention Group ◽  
Oocyte Quality ◽  
Metabolic Parameters ◽  
Control Group ◽  
Obese Women ◽  
High Fat ◽  
Obesogenic Diet

Obesity adversely affects reproduction and results in oocyte defects in both mice and humans. In the present study we used a mouse model to examine whether the adverse effects of an obesogenic diet on oocyte metabolism and morphology can be reversed by return to a control diet. The intervention group consisted of C57BL6/J mice placed on a high-fat diet (HFD; 35.8% fat and 20.2% protein by nutritional content) for 6 weeks and then switched to an isocaloric control diet (CD; 13% fat and 25% protein) for 8 weeks (HFD/CD mice). The control group consisted of age-matched C57BL6/J mice maintained on CD for 14 weeks (CD/CD mice). Although metabolic parameters (weight, glucose tolerance and cholesterol levels) of HFD/CD mice returned to normal after this ‘diet reversal’ period, several oocyte defects were not reversible. These HFD/CD oocytes demonstrated significantly higher percentages of abnormal meiotic spindles, lower mitochondrial membrane potential and lower ATP and citrate levels, and higher percentages of abnormal lipid accumulation and mitochondrial distribution compared with CD/CD mice. These results suggest that the negative effects of an obesogenic diet on oocyte quality are not reversible, despite reversal of metabolic parameters. These data may provide better insight when counselling obese women regarding reproductive options and success.

scholarly journals Melatonin Modulates Lipid Metabolism in Porcine Cumulus–Oocyte Complex via Its Receptors

2021 ◽  
Vol 9 ◽  
Author(s):  
Tianqi Zhu ◽  
Shengyu Guan ◽  
Dongying Lv ◽  
Mengmeng Zhao ◽  
Laiqing Yan ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Energy Resource ◽  
Cumulus Cells ◽  
Melatonin Receptors ◽  
Developmental Competence ◽  
General Level ◽  
Cumulus Oocyte Complex ◽  
Triacsin C ◽  
Porcine Oocyte

Lipid is a crucial energy resource for mammalian oocyte. Melatonin could benefit the maturation of porcine oocyte in vitro, but the related mechanism is not elucidated yet. In the current study, methods to monitor lipid metabolism in single live oocytes were firstly established using probes (Lipi-Blue and Lipi-Green). It was observed that both lipid biogenesis and lipolysis occurred in maturing oocyte, but the general level of lipids dropped. Then maturing oocytes stained with probes were treated with melatonin or lipid metabolic-related inhibitors (triacsin C, rotenone, or etomoxir). The results showed that the lipid metabolism and maturation of porcine oocytes were all disrupted and that melatonin rescued the oocytes treated with triacsin C or rotenone, but not those treated with etomoxir. Further investigation demonstrated that cumulus cells are able to transfer lipids to oocytes via gap junctions. It was also observed that melatonin receptors exist in cumulus cells and are required for oocytes to maintain lipid metabolism. Meanwhile, the global gene expressing in cumulus cells was also modulated by melatonin, especially the genes related to antioxidants (SOD1, GPX1, GPX3, GPX4, PRDX2, and PRDX5), lipid metabolism (FABP3, FABP5, ACACB, TECR, etc.), and mitochondrial respiration (GPD1, ETFB, CYC1, and the genes of ATP synthase). Altogether the current research demonstrates that melatonin modulates lipid metabolism in maturing oocytes through its receptors in cumulus cells and benefits the developmental competence of oocytes.

scholarly journals Changes in serum cholinesterase (EC 3 1 * 1 * 8) activity in rats consuming a high-fat diet

1989 ◽  
Vol 62 (2) ◽  
pp. 343-348 ◽  
Author(s):  
Jesus Osada ◽  
Hortensia Aylagas ◽  
Gonzalo Cao ◽  
Maria Jesus MIRo−Obradors ◽  
Evangelina Palacios−Alaiz
Keyword(s):  
Lipid Metabolism ◽  
High Fat Diet ◽  
Intraperitoneal Injection ◽  
Control Diet ◽  
Male Rats ◽  
Serum Cholinesterase ◽  
High Fat ◽  
Dietary Components ◽  
Apparent Susceptibility ◽  
Daily Intraperitoneal Injection

Adult male rats were fed on a control diet containing (g/kg) carbohydrate 600, lipid 35 and protein 190, or on a high-fat diet containing carbohydrate 360, lipid 420 and protein 120. After 30 d, the high-fat diet provoked a decrease in serum cholinesterase (EC3.1.1.8) activity which was reversed by feeding rats on the control diet. The observed decrease after 90 d on the high-fat diet was not seen if a simultaneous daily intraperitoneal injection of a lipotrophic agent containing (mg/kg) S-adenosyl-l-methionine 3, coenzyme A 0.1, UDP-glucose 30 and CDP-choline 1.5 was given to rats on the high-fat diet. The findings are discussed in relation to the apparent susceptibility of serum cholinesterase to dietary components and its possible role in lipid metabolism.

Effect of Brassica rapa Sprouts on Lipid Metabolism in Rats Fed High Fat Diet

2010 ◽  
Vol 39 (5) ◽  
pp. 669-676 ◽  
Author(s):  
Jae-Joon Lee ◽  
Tae-Man Ha ◽  
Yu-Mi Lee ◽  
Ah-Ra Kim ◽  
Myung-Yul Lee
Keyword(s):  
Lipid Metabolism ◽  
Brassica Rapa ◽  
High Fat Diet ◽  
High Fat

Anti-obesity effects of Grifola frondosa through the modulation of lipid metabolism via ceramide in mice fed a high-fat diet

2021 ◽  
Author(s):  
Xue Jiang ◽  
Jie Hao ◽  
Zijian Liu ◽  
Xueting Ma ◽  
Yuxin Feng ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Lipid Metabolism ◽  
High Fat Diet ◽  
Fat Deposition ◽  
Grifola Frondosa ◽  
High Fat ◽  
Metabolic Complications ◽  
Basidiomycete Fungus

Obesity is characterized by massive fat deposition and is related to a series of metabolic complications, such as insulin resistance (IR) and steatohepatitis. Grifola frondosa (GF) is a basidiomycete fungus...

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