Genetic variation within the dasyurid marsupial genus Planigale

2000 ◽  
Vol 48 (5) ◽  
pp. 443 ◽  
Author(s):  
Mark J. Blacket ◽  
Mark Adams ◽  
Carey Krajewski ◽  
Michael Westerman
Keyword(s):  
Genetic Variation ◽  
South Australia ◽  
Taxonomic Diversity ◽  
12S Rrna ◽  
Rrna Gene ◽  
Widespread Species ◽  
Geographic Ranges ◽  
12S Rrna Gene ◽  
The Status ◽  
Taxonomic Changes

Genetic variation within the genus Planigale was examined through analyses of 12S rRNA gene sequences and allozymes. The level of genetic divergence between the five currently recognised Planigale species was compared and the magnitude of divergence among populations assessed. This examination of molecular variation within the genus revealed that Planigale contains far more taxonomic diversity than is currently recognised. Specifically, the Pilbara region of Western Australia probably contains two currently unrecognised Planigale species and there is substantial genetic heterogeneity within the widespread species P. maculata. Ambiguity over the status of several genetic and/or morphological forms within the genus indicates that further taxonomic changes are likely to be warranted in the future. This study also demonstrates that the currently accepted geographic ranges of many planigale species require careful re-evaluation and that many specimens in collections are apparently misidentified. This is especially true of P. ingrami, which appears to have a much greater range than is currently recognised, being present in South Australia.

Molecular review of the genus Panaspis sensu lato (Reptilia: Scincidae) in Cameroon, with special reference to the status of the proposed subgenera

Zootaxa ◽  
2005 ◽  
Vol 863 (1) ◽  
pp. 1 ◽  
Author(s):  
ANDREAS SCHMITZ ◽  
IVAN INEICH ◽  
LAURENT CHIRIO
Keyword(s):  
Genetic Variation ◽  
Phylogenetic Inference ◽  
12S Rrna ◽  
Rrna Gene ◽  
Likelihood Methods ◽  
Morphological Studies ◽  
The Status ◽  
Maximum Likelihood Methods ◽  
Taxonomic Changes ◽  
Mitochondrial 12S Rrna

The scincid taxa connected to the genus Panaspis sensu lato have undergone various taxonomic changes based mainly on morphological studies in the last century, but their true relationships are still far from clear. In the present study we applied Bayesian, maximum parsimony, and maximum likelihood methods of phylogenetic inference to mitochondrial 12S rRNA and 16S rRNA gene fragments to examine the degree of genetic variation within the genus. We found considerable genetic differentiation between the different proposed subgenera. Similarly, we could show that two species considered to be part of the former subgenus Leptosiaphos are in reality genuine members of the genus Lacertaspis. Based on our results we propose the recognition of the four former subgenera as full genera, and we identify several additional, possibly subgeneric, lineages within the different newly recognized full genera.

scholarly journals Genetic variation and phylogenetic analysis of Indonesian indigenous catfish (baung fish) based on mitochondrial 12S rRNA gene

2021 ◽  
Vol 14 (3) ◽  
pp. 751-757
Author(s):  
Rini Widayanti ◽  
Ken Ayik Kusumaastuti ◽  
Joana Martha Novi ◽  
Fadila Khairuna Adani ◽  
Catrine Relia Patrecia Gultom ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Phylogenetic Relationships ◽  
Population Based ◽  
Genetic Distances ◽  
12S Rrna ◽  
Rrna Gene ◽  
Mystus Vittatus ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

Background and Aim: Baung fish is an essential commodity in Indonesia; however, few studies have explored the genetic diversity of Indonesian catfish. Thus, this study aimed to analyze the genetic variation and phylogenetic relationships among Indonesian catfish based on the mitochondrial 12S ribosomal RNA (rRNA) gene. Materials and Methods: In total, 28 catfish were collected from nine rivers in seven provinces and from the Indian Ocean. Catfish genomes were obtained from epaxial and hepaxial muscle samples. The mitochondrial 12S rRNA gene was amplified by polymerase chain reaction using a pair of primers (Baung12SF and Baung12SR). The 12S rRNA sequences were analyzed using MEGA X to determine genetic variation and phylogenetic relationships. Results: In total, 178 variation sites in the 12S rRNA gene were substituted among Indonesian catfish. The genetic distance between all Indonesian catfish samples was 0.1-16.0%. The closest genetic distance was between MP and PM catfish, whereas the farthest genetic distances were between BF and EM and PD and EM. For the entire population, based on mean diversity calculations, the number of base substitutions per site was 0.08. Conclusion: Indonesian catfish were divided into four clades based on the 12S rRNA gene. The catfish MP, KR, PM, MS, BB, and KS were grouped with Hemibagrus nemurus, the catfish EM was grouped with Mystus vittatus, the catfish BSBJ was grouped with Pangasius pangasius, and the catfish PD and BF were grouped with Netuma thalassina.

scholarly journals First Molecular Confirmation of Equine Ocular Setaria digitata in China

2021 ◽  
Vol 8 (4) ◽  
pp. 55
Author(s):  
Feng Yu ◽  
Bo Liu ◽  
Shulei Chen ◽  
Ziwen Yi ◽  
Xianyong Liu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Light Microscopy ◽  
Corneal Opacity ◽  
12S Rrna ◽  
Rrna Gene ◽  
Asian Countries ◽  
Setaria Digitata ◽  
12S Rrna Gene ◽  
The Right ◽  
Ocular Discharge

A 5-year-old Mongolian mare (Equus caballus Linnaeus, 1758) was observed to have corneal opacity and excessive ocular discharge. An ophthalmic examination revealed a moving thread-like cylindrical worm in the anterior chamber of the right eye. The parasite was successfully removed surgically. The worm was observed under light microscopy and confirmed as Setaria digitata by 12S rRNA gene amplification and sequencing. Phylogenetic analysis demonstrated similarity with Setaria digitata in the National Center for Biotechnology Information (NCBI) GenBank database isolated from other Asian countries. This report is the first confirmed case of equine ocular setariasis by molecular diagnosis in China, which may indicate its presence in livestock and promote research on its epidemiology.

scholarly journals Markers of Genetic Variation in Blue Gourami (Trichogaster trichopterus) as a Model for Labyrinth Fish

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 228
Author(s):  
Gad Degani ◽  
Isana Veksler-Lublinsky ◽  
Ari Meerson
Keyword(s):  
Growth Hormone ◽  
Genetic Variation ◽  
Genetic Markers ◽  
Basic Research ◽  
Coi Gene ◽  
12S Rrna ◽  
Rrna Gene ◽  
Somatotropic Axis ◽  
Genes Encoding ◽  
Growth And Reproduction

Markers of genetic variation between species are important for both applied and basic research. Here, various genes of the blue gourami (Trichogaster trichopterus, suborder Anabantoidei, a model labyrinth fish), many of them involved in growth and reproduction, are reviewed as markers of genetic variation. The genes encoding the following hormones are described: kisspeptins 1 and 2, gonadotropin-releasing hormones 1, 2, and 3, growth hormone, somatolactin, prolactin, follicle- stimulating hormone and luteinizing hormone, as well as mitochondrial genes encoding cytochrome b and 12S rRNA. Genetic markers in blue gourami, representing the suborder Anabantoidei, differ from those in other bony fishes. The sequence of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene of blue gourami is often used to study the Anabantoidei suborder. Among the genes involved in controlling growth and reproduction, the most suitable genetic markers for distinguishing between species of the Anabantoidei have functions in the hypothalamic–pituitary–somatotropic axis: pituitary adenylate cyclase-activating polypeptide and growth hormone, and the 12S rRNA gene.

Mitochondrial 12S rRNA gene mutations affect RNA secondary structure and lead to variable penetrance in hearing impairment

2006 ◽  
Vol 341 (4) ◽  
pp. 950-957 ◽  
Author(s):  
Ester Ballana ◽  
Estela Morales ◽  
Raquel Rabionet ◽  
Bàrbara Montserrat ◽  
Marina Ventayol ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Hearing Impairment ◽  
Rna Secondary Structure ◽  
Gene Mutations ◽  
12S Rrna ◽  
Rrna Gene ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna ◽  
Variable Penetrance

Maternally transmitted aminoglycoside-induced and non-syndromic hearing loss caused by the 1494C > T mutation in the mitochondrial 12S rRNA gene in two Chinese families

2012 ◽  
Vol 52 (2) ◽  
pp. 98-103 ◽  
Author(s):  
Qinjun Wei ◽  
Dan Xu ◽  
Zhibin Chen ◽  
Haifeng Li ◽  
Yajie Lu ◽  
...  
Keyword(s):  
Hearing Loss ◽  
12S Rrna ◽  
Rrna Gene ◽  
Chinese Families ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna ◽  
Syndromic Hearing Loss

Phylogenetic Relationships among the Species of the Genus Testudo (Testudines: Testudinidae) Inferred from Mitochondrial 12S rRNA Gene Sequences

2002 ◽  
Vol 22 (2) ◽  
pp. 174-183 ◽  
Author(s):  
Antoinette C. van der Kuyl ◽  
Donato L. Ph. Ballasina ◽  
John T. Dekker ◽  
Jolanda Maas ◽  
Ronald E. Willemsen ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
12S Rrna ◽  
Rrna Gene ◽  
Gene Sequences ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

scholarly journals Evaluation and utility of mitochondrial ribosomal genes for molecular systematics of parasitic nematodes

2020 ◽  
Author(s):  
Abigail Hui En Chan ◽  
Kittipong Chaisiri ◽  
Serge Morand ◽  
Naowarat Saralamba ◽  
Urusa Thaenkham
Keyword(s):  
Genetic Marker ◽  
Genetic Markers ◽  
Molecular Systematics ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
16S Ribosomal Rna ◽  
12S Rrna ◽  
Rrna Gene ◽  
12S Rrna Gene ◽  
Rna Genes

Abstract Background Molecular advances have accelerated our understanding of nematode systematics and taxonomy. However, comparative analyzes between various genetic markers have led to discrepancies in nematode phylogenies. This study aimed to evaluate the suitability of using mitochondrial 12S and 16S ribosomal RNA genes for nematode molecular systematics. Methods To study the suitability of mitochondrial 12S and 16S ribosomal RNA genes as genetic markers for nematode molecular systematics, we compared them with the other commonly used genetic markers, nuclear internal transcribed spacer 1 and 2 regions, nuclear 18S and 28S ribosomal RNA genes, and mitochondrial cytochrome c oxidase subunit 1 gene. After that, phylum-wide primers for mitochondrial 12S and 16S ribosomal RNA genes were designed, and parasitic nematodes of humans and animals from 75 taxa with 21 representative species were inferred through phylogenetic analyzes. Phylogenetic analyzes were carried out using maximum likelihood and Bayesian inference algorithms. Results The phylogenetic relationships of nematodes based on the mitochondrial 12S rRNA gene supported the monophyly of nematodes in clades I, IV, and V, reinforcing the potential of this gene as a genetic marker for nematode systematics. In contrast, the mitochondrial 16S rRNA gene only supported the monophyly of clades I and V, providing evidence that the 12S rRNA gene is more suitable for nematode molecular systematics. In this study, subclades of clade III containing various nematode families were not monophyletic when the 16S or 12S rRNA gene was used as the genetic marker. This is similar to the phylogenetic relationship revealed by previous studies using whole mitochondrial genomes as genetic markers. Conclusions This study supports the use of the 12S rRNA gene as a genetic marker for studying the molecular systematics of nematodes to understand intra-phyla relationships. Phylum-wide primers for nematodes using mitochondrial ribosomal genes were prepared, which may enhance future studies. Furthermore, sufficient genetic variation in the mitochondrial 12S and 16S rRNA genes between species also allowed for accurate taxonomy to species level, revealing the potential of these two genes as genetic markers for DNA barcoding.

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