scholarly journals Label-free hematology analysis using deep-ultraviolet microscopy

2020 ◽  
Vol 117 (26) ◽  
pp. 14779-14789 ◽  
Author(s):  
Ashkan Ojaghi ◽  
Gabriel Carrazana ◽  
Christina Caruso ◽  
Asad Abbas ◽  
David R. Myers ◽  
...  
Keyword(s):  
Blood Cell ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Live Cells ◽  
Label Free ◽  
Diagnostic Efficacy ◽  
Ultraviolet Microscopy ◽  
Blood Smears ◽  
Hematological Analysis ◽  
Deep Ultraviolet

Hematological analysis, via a complete blood count (CBC) and microscopy, is critical for screening, diagnosing, and monitoring blood conditions and diseases but requires complex equipment, multiple chemical reagents, laborious system calibration and procedures, and highly trained personnel for operation. Here we introduce a hematological assay based on label-free molecular imaging with deep-ultraviolet microscopy that can provide fast quantitative information of key hematological parameters to facilitate and improve hematological analysis. We demonstrate that this label-free approach yields 1) a quantitative five-part white blood cell differential, 2) quantitative red blood cell and hemoglobin characterization, 3) clear identification of platelets, and 4) detailed subcellular morphology. Analysis of tens of thousands of live cells is achieved in minutes without any sample preparation. Finally, we introduce a pseudocolorization scheme that accurately recapitulates the appearance of cells under conventional staining protocols for microscopic analysis of blood smears and bone marrow aspirates. Diagnostic efficacy is evaluated by a panel of hematologists performing a blind analysis of blood smears from healthy donors and thrombocytopenic and sickle cell disease patients. This work has significant implications toward simplifying and improving CBC and blood smear analysis, which is currently performed manually via bright-field microscopy, and toward the development of a low-cost, easy-to-use, and fast hematological analyzer as a point-of-care device and for low-resource settings.

scholarly journals M2G1G2 white blood cell flag by three-part automated hematology analyzer: A hint to dengue infection in appropriate clinical context

2019 ◽  
Vol 11 (02) ◽  
pp. 103-106
Author(s):  
Maitrayee Roy ◽  
Akshay Bali
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Dengue Infection ◽  
Blood Film ◽  
Clinical Context ◽  
Normal Limits ◽  
Hematology Analyzer ◽  
Automated Hematology Analyzer

Abstract BACKGROUND: Automated hematology analyzers often generate many flags which can provide important clues to the underlying hematological abnormality. Although pathologists are generally well versed in recognizing the importance of flags indicating potential leukemic blasts, their utility in hinting toward infectious etiology, especially during epidemics, is less well known. We analyzed any abnormal flags generated by a three-part automated hematology analyzer in serologically confirmed cases of dengue. MATERIALS AND METHODS: The study included 28 patients diagnosed with serologically confirmed dengue infection. The venous samples were run on ABX Miros-60 three-part hematology analyzer. The complete blood count data and any abnormal flags were noted and correlated with peripheral blood film findings in all patients. RESULTS: An abnormal white blood cell (WBC) flag was noted in all 28 patients, including two in whom all other hematological parameters were within normal limits. In 26 (93%) patients, M2G1G2 WBC flag was noted while the samples of the remaining two patients generated M2G1 and L1M2G1G2 WBC flags, respectively. CONCLUSION: An abnormal WBC flag, when correlated in appropriate clinical context, especially during a dengue outbreak, can aid in targeting the cohort of patients who will require immediate referral for serological confirmation.

Tunisian Newborn's Cord Blood: Reference Values of Complete Blood Count and Hemoglobin Fractions

2020 ◽  
Author(s):  
Sana Zitouni ◽  
Emna Bouatrous ◽  
Ons Laabidi ◽  
Imen Boudrigua ◽  
Dorra Chaouachi ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cord Blood ◽  
Reference Values ◽  
Blood Count ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Reference Ranges ◽  
Hematological Indices ◽  
Umbilical Blood ◽  
Vaginal Deliveries

Objective This study was aimed to establish local reference values for hematological indices and hemoglobin (Hb) fractions in umbilical cord blood (UCB) for the northern population of Tunisia. Study Design Our study included full-term newborns by vaginal deliveries. Hematological parameters were collected using an automated blood cell counter. The amounts of Hb fractions were measured by capillary electrophoresis of Hb. Statistical analysis was performed using R software. Results A total of 328 cord blood samples were analyzed. Among them, 154 (male: 44.8%, female: 55.2%) were used to establish reference values. The normal reference values of complete blood count (CBC) and Hb fractions were calculated. Mean neonatal Hb was 14.75 ± 2.26 g/dL. Gestational age affects the expression of CBC values as red blood cell (RBC), Hb, hematocrit (Hct), mean corpuscular volume (MCV), white blood cell (WBC), and the Hb profile. Umbilical blood hemogram parameters and Hb profile are affected by the environment; higher in newborns from urban regions but not affected by gender ratio. Conclusion Reference ranges of normal CBC indices and Hb fractions have been successfully established in Tunisian neonates' UCB. Our data suggest reference values that could be useful for neonatal patients' laboratory results and clinical interpretation. Key Points

scholarly journals A comparative study of hematological parameters between hypertensive and normotensive individuals in Harar, eastern Ethiopia

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260751
Author(s):  
Beza Sileshi ◽  
Fekadu Urgessa ◽  
Moges Wordofa
Keyword(s):  
Blood Cell ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
University Hospital ◽  
Health Concern ◽  
Control Group ◽  
Eastern Ethiopia ◽  
Distribution Width ◽  
Wbc Count ◽  
Apparently Healthy

Background Hypertension is the major public health concern; leading to cardiovascular disease. It is associated with alteration in hematological parameters which may lead to end-organ damage. Thus, this study aimed to compare hematological parameters between hypertensive and normotensive adult groups in Harar, eastern Ethiopia. Methods A comparative cross-sectional study was conducted from January to March, 2020 at Jugel and Hiwotfana Specialized University hospital, Harar, eastern Ethiopia. Convenient sampling technique was used to recruit 102 hypertensive patients from the two hospitals and 102 apparently healthy blood donors. Participant’s socio-demographic and clinical information were collected using pre-tested structured questionnaire. Blood sample were collected and analyzed by Beckman Coulter DxH 500 analyzer for complete blood count. The data were entered and analyzed using SPSS version 23. Independent t-test and Mann Whitney u-test was used for comparison between groups. Spearman’s correlation was used for correlation test. P values less than 0.05 was considered as statistically significant. Result 102 hypertensive and 102 healthy controls were enrolled in this study. The median ± IQR value of white blood cell (WBC) count, hemoglobin (Hgb), hematocrit (HCT), red cell distribution width (RDW) and mean platelet volume (MPV) were significantly higher in hypertensive group compared to apparently healthy control group. Additionally, RBC (red blood cell) count, HCT and RDW showed statistically significant positive correlations with systolic and diastolic blood pressure. WBC count and RDW were significantly and positively correlated with body mass index (BMI). Platelet (PLT) count had a significant but negative correlation (r = -0.219, P = 0.027) with duration of hypertension illness while MPV showed positive and significant correlation (r = 0.255, P = 0.010). Conclusion The median values of WBC, Hgb, HCT, RDW and MPV were significantly higher in hypertensive patient compared to apparently healthy individuals. Hence, it is important to assess hematological parameters for hypertensive individuals which may help to prevent complications associated with hematological aberrations. However, further studies are required to understand hypertensive associated changes in hematological parameters.

scholarly journals Hematological status and functional abilities of the cardiorespiratory system of physically active female vegans

2020 ◽  
Vol 74 (1) ◽  
pp. 39-46
Author(s):  
Vladimir Ilić ◽  
Slobodanka Dobrijević ◽  
Nemanja Rebić
Keyword(s):  
Complete Blood Count ◽  
Hematological Parameters ◽  
Maximal Oxygen Consumption ◽  
Vegan Diet ◽  
Control Group ◽  
Standard Diet ◽  
Functional Abilities ◽  
Cardiorespiratory System ◽  
Blood Lipid Profile ◽  
Hematological Analysis

The aim of this study was to examine the influence of vegan diet on hematological parameters, and to compare the functional abilities of the cardiorespiratory system of vegans and counterparts on a standard diet. The sample consisted of 24 females, divided into two groups: 11 vegans and 13 women on a standard diet. Assessment of the level of physical activity of the participants was estimated by the standard IPAQ questionnaire. Hematological analysis included: complete blood count, blood lipid profile, vitamin B12 and iron. Basic anthropometric variables were monitored, and a submaximal Astrand test on a bicycle ergometer was used to estimate maximal oxygen consumption. The RAMP continuous protocol was used to measure the maximum values of heart rate and blood pressure. The results showed that active vegans have a significantly lower body weight and body mass index compared to active women on a standard diet. The number of erythrocytes and platelets, as well as LDL - cholesterol show lower values in vegans compared to the control group (p<0.01). Indicators of the functional abilities of the cardiorespiratory system do not differ between active vegans and women on a standard diet.

Synchrotron UV Fluorescence Microscopy Uncovers New Probes in Cells and Tissues

2010 ◽  
Vol 16 (5) ◽  
pp. 507-514 ◽  
Author(s):  
Frederic Jamme ◽  
Sandrine Villette ◽  
Alexandre Giuliani ◽  
Valerie Rouam ◽  
Frank Wien ◽  
...  
Keyword(s):  
High Resolution ◽  
Wavelength Range ◽  
Cell Biology ◽  
Live Cells ◽  
Label Free ◽  
Full Spectrum ◽  
The Past ◽  
Good Spatial Resolution ◽  
Deep Ultraviolet ◽  
Microscopic World

AbstractUse of deep ultraviolet (DUV, below 350 nm) fluorescence opens up new possibilities in biology because it does not need external specific probes or labeling but instead allows use of the intrinsic fluorescence that exists for many biomolecules when excited in this wavelength range. Indeed, observation of label free biomolecules or active drugs ensures that the label will not modify the biolocalization or any of its properties. In the past, it has not been easy to accomplish DUV fluorescence imaging due to limited sources and to microscope optics. Two worlds were coexisting: the spectrofluorometric measurements with full spectrum information with DUV excitation, which lacked high-resolution localization, and the microscopic world with very good spatial resolution but poor spectral resolution for which the wavelength range was limited to 350 nm. To combine the advantages of both worlds, we have developed a DUV fluorescence microscope for cell biology coupled to a synchrotron beamline, providing fine tunable excitation from 180 to 600 nm and full spectrum acquired on each point of the image, to study DUV excited fluorescence emitted from nanovolumes directly inside live cells or tissue biopsies.

scholarly journals Clinician-ordered peripheral blood smears have low reimbursement and variable clinical value: a three-institution study, with suggestions for operational efficiency

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Amy K. Beckman ◽  
Valerie L. Ng ◽  
David L. Jaye ◽  
Manila Gaddh ◽  
Sarah A. Williams ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Complete Blood Count ◽  
Clinical Value ◽  
Laboratory Staff ◽  
Clinical Scenarios ◽  
Blood Smears ◽  
Peripheral Blood Smears

Abstract Background Peripheral blood smears are performed to evaluate a variety of hematologic and non-hematologic disorders. At the authors’ institutions, clinician requests for pathologist-performed blood smear reviews have increased in recent years. Blood smears may contribute significantly to pathologists’ workloads, yet their clinical value is variable, and professional reimbursement rates are low. This study aimed to identify clinical scenarios in which smear review is likely to provide value beyond automated laboratory testing. Methods Blood smear review practices at three institutions were examined, and the indications for and interpretations of clinician-initiated smears were reviewed to determine the percentage of smears with potential added clinical value. A smear review was classified as having added clinical value if the pathologist’s interpretation included a morphologic abnormality that had the potential to impact patient management, and that could not be diagnosed by automated complete blood count with white blood cell differential or automated iron studies alone. Results Among 515 consecutive clinician-requested smears performed during the study timeframes, 23% yielded interpretations with potential added clinical value. When sorted by indication, 25, 19, and 13% of smear reviews requested for white blood cell abnormalities, red blood cell abnormalities, and platelet abnormalities, respectively, had findings with potential added clinical value. The proportion of smears with potential clinical value differed significantly across these three categories (p = 0.0375). Conclusions Smear review ordering practices across three institutions resulted in a minority of smears with potential added clinical value. The likelihood of value varied according to the indication for which the smear was requested. Given this, efforts to improve the utilization and efficiency of smear review are worthwhile. Solutions are discussed, including engaging laboratory staff, educating clinicians, and modifying technology systems.

scholarly journals Therapeutic efficacy and safety evaluation of erythrocyte concentrate used in dogs

2016 ◽  
Vol 73 (2) ◽  
pp. 399
Author(s):  
Ildiko Barabasi ◽  
Cristina Stefanut ◽  
Laurent Ognean
Keyword(s):  
Statistical Analysis ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Therapeutic Efficacy ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Safety Evaluation ◽  
Efficacy And Safety ◽  
Transfusion Therapy ◽  
Test Kit

Therapeutic efficacy and safety evaluation of erythrocyte concentrate used in dogs 1Ildikó BARABÁSI, 1Cristina ȘTEFǍNUȚ, 1Laurenţ OGNEAN 1University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400037, Manastur street, no.3-5, Cluj-Napoca, Romania *Corresponding author: [email protected]   Keywords: dogs, erythrocyte concentrate, hematocrit, immune-mediated hemolytic anemia, transfusion therapy Introduction: The minimum dose of whole blood products as well as erythrocyte concentrate has been under a lot of debate, new equations for calculating the optimal dose being made up from a large variety of hematologists (Kisielewicz et al 2014; Helm and Knottenbelt, 2010; Gibson, 2007). Aim: The therapeutical efficacy of erythrocyte concentrates in dogs with different types of anemia by measuring the hematocrit level 6 hours after the transfusion and a complete blood count 5 days post-transfusion therapy. Materials and methods: Blood tests were performed with ADIVA hematological analyzer; the 6 hour post-transfusion hematocrit was determined by a micro hematocrit. On admission every patient received a routine blood test that included 40 hematological parameters and 21 biochemical parameters. In addition, a detailed examination of the blood smears was also performed by the ADIVA hematological analyzer with 26 parameters that mostly referred to red blood cell and white blood cell morphology. Blood typing was done using the RapidVet quick test kit. Patients received only type specific blood and to limit transfusion reaction occurrences, in addition, a crossmatch test was performed before every transfusion. Statistical analysis was accomplished with GraphPadInStat 3.0 and the graphical depiction of the obtained results was made using the Origin 8.5. graphics program. Results: Statistical analysis reveal that the total red blood cell count underwent very significant changes (p=0.0052) as well as the hemoglobin (p=0.0085). The hematocrit had an extremely significant evolution (p=0.0002). As far as the other hematological parameters are concerned, none underwent statistically significant evolutions from first day of transfusion (T0) until five days post-transfusion therapy (T5). Conclusion: The erythrocyte concentrate can be used safely even in critically ill or immune-suppressed patients and even in patients with an exaggerated immune response. A clear dosage of this blood product has not been set yet, every administration has to be tailored to the patient’s needs.

scholarly journals Label-free automated neutropenia detection and grading using deep-ultraviolet microscopy

2021 ◽  
Author(s):  
Ashkan Ojaghi ◽  
Paloma Casteleiro Costa ◽  
Christina Caruso ◽  
wilbur lam ◽  
Francisco Robles
Keyword(s):  
Label Free ◽  
Ultraviolet Microscopy ◽  
Deep Ultraviolet
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