scholarly journals Molecular Analysis of Murine Leukemia Cell Lines Resistant to 5,10-Dideazatetrahydrofolate Identifies Several Amino Acids Critical to the Function of Folylpolyglutamate Synthetase

2000 ◽  
Vol 275 (34) ◽  
pp. 26599-26606 ◽  
Author(s):  
Rongbao Zhao ◽  
Steven Titus ◽  
Feng Gao ◽  
Richard G. Moran ◽  
I. David Goldman
Keyword(s):  
Amino Acids ◽  
Cell Lines ◽  
Molecular Analysis ◽  
Leukemia Cell ◽  
Folylpolyglutamate Synthetase ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Murine Leukemia

Comparison of cytotoxicity and cellular accumulation of polynuclear platinum complexes in L1210 murine leukemia cell lines

1999 ◽  
Vol 77 (1-2) ◽  
pp. 47-50 ◽  
Author(s):  
John D. Roberts ◽  
John Peroutka ◽  
G. Beggiolin ◽  
C. Manzotti ◽  
L. Piazzoni ◽  
...  
Keyword(s):  
Cell Lines ◽  
Leukemia Cell ◽  
Platinum Complexes ◽  
Cellular Accumulation ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Murine Leukemia

scholarly journals Antiproliferative Activity of Triterpenoid and Steroid Compounds from Ethyl Acetate Extract of Calotropis gigantea Root Bark against P388 Murine Leukemia Cell Lines

2021 ◽  
Vol 89 (2) ◽  
pp. 21
Author(s):  
Kartini Hasballah ◽  
Murniana Sarong ◽  
Renzavaldy Rusly ◽  
Herdina Fitria ◽  
Dewi Rara Maida ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Ethyl Acetate Extract ◽  
Leukemia Cell ◽  
Root Bark ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Murine Leukemia ◽  
Antiproliferative Activities

Calotropis gigantea has been known to produce bioactive secondary metabolites with antiproliferative activities against cancer cells. Herein, we extracted the secondary metabolites using ethyl acetate from its root bark and further tested its antiproliferative activities against P388 murine leukemia cell lines. The subfractions from the ethyl acetate extract was obtained from Vacuum Liquid Column Chromatography (VLCC), and followed by Gravity Column Chromatography (GCC). The subfraction C2 and D1 were identified to contain triterpenoids and steroids with the most potent cytotoxicity against Brine Shrimp Lethality Test (BSLT). A 3-(4,5-dimethylthiazol-2-yl) -2-5 diphenyl tetrazolium bromide (MTT) assay suggested that ethyl acetate extract has the highest antiproliferative activities against P388 murine leukemia cell lines (IC50 = 21.79 μg/mL), as opposed to subfraction C2 (IC50 = 50.64 µg/mL) and subfraction D1 (IC50 = 49.33 µg/mL). The compound identified in subfraction C2 and D1 are taraxerol acetate and calotropone, respectively. Though taraxerol acetate and calotropone were active in inhibiting the leukemic cell lines, their IC50s were lower than the ethyl acetate extract, which is probably due to the synergism of the secondary metabolites.

Establishment of a hemopoietic stimulating factor producing murine leukemia cell lines: Pathogenesis of granulocytosis in L8313 bearing mice

1988 ◽  
Vol 12 (9) ◽  
pp. 763-771 ◽  
Author(s):  
Hitoshi Sawada ◽  
Katsuhiko Itoh ◽  
Teruo Kirikae ◽  
Hiroto Sakoda ◽  
Hiroaki Tezuka ◽  
...  
Keyword(s):  
Cell Lines ◽  
Leukemia Cell ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Stimulating Factor ◽  
Murine Leukemia

scholarly journals Anti-leukemic properties of deferasirox via apoptosis in murine leukemia cell lines

2015 ◽  
Vol 50 (1) ◽  
pp. 33 ◽  
Author(s):  
Sol-Rim Jeon ◽  
Jae-Wook Lee ◽  
Pil-Sang Jang ◽  
Nack-Gyun Chung ◽  
Bin Cho ◽  
...  
Keyword(s):  
Cell Lines ◽  
Leukemia Cell ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Murine Leukemia

Disparate Mechanisms of Antifolate Resistance Provoked by Methotrexate and Its Metabolite 7-Hydroxymethotrexate in Leukemia Cells: Implications for Efficacy of Methotrexate Therapy.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4369-4369
Author(s):  
Freidoun Albertioni ◽  
Gerrit Jansen ◽  
Kambiz Fotoohi ◽  
Yehuda G. Assaraf ◽  
Lilah Rothem ◽  
...  
Keyword(s):  
Cell Lines ◽  
Leukemia Cell ◽  
Parent Drug ◽  
Underlying Mechanism ◽  
Human Leukemia ◽  
Folylpolyglutamate Synthetase ◽  
Leukemia Cell Lines ◽  
Short Term Exposure ◽  
Extensive Metabolism ◽  
Antifolate Resistance

Abstract Methotrexate (MTX) is one of the leading drugs in the treatment of leukemia, but extensive metabolism to 7-hydroxymethotrexate (7-OHMTX) can limit its therapeutic efficacy. In this study we investigated whether 7-OHMTX itself can provoke antifolate resistance that may further disrupt MTX efficacy. For this purpose we developed resistance to 7-OHMTX as well as MTX in two human leukemia cell lines (CCRF-CEM and MOLT-4) by stepwise exposure to increasing concentrations of 7-OHMTX and MTX. Consequently, both leukemia cell lines displayed marked levels of resistance to 7-OHMTX (>10 fold) and MTX (>75 fold), respectively. The underlying mechanism of resistance in the MTX-exposed cells was a marked decrease (>10-fold) in reduced folate carrier (RFC)-mediated cellular uptake of MTX. This was associated with transcriptional silencing of the RFC gene in MTX-resistant CCRF-CEM cells. In contrast, the molecular basis for the resistance to 7-OHMTX was solely due to a marked decreased (> 95%) in folylpolyglutamate synthetase (FPGS) activity which conferred >100-fold MTX resistance upon a short term exposure to this drug. This is the first demonstration that 7-OHMTX can provoke distinct modalities of antifolate resistance as compared to the parent drug MTX.

Phenotypic and molecular analysis of ph1-chromosome-positive acute lymphoblastic leukemia cell lines

1993 ◽  
Vol 53 (3) ◽  
pp. 457-462 ◽  
Author(s):  
T. Miyagi ◽  
J. Ohyashiki ◽  
K. Yamato ◽  
H. P. Koeffler ◽  
I. Miyoshi
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Cell Lines ◽  
Molecular Analysis ◽  
Lymphoblastic Leukemia ◽  
Leukemia Cell ◽  
Leukemia Cell Lines

scholarly journals Triterpenoids from the Bark of Aglaia glabrata and their In vitro Effects on P-388 Murine Leukemia Cells

2019 ◽  
Vol 35 (1) ◽  
pp. 134-139
Author(s):  
Desi Harneti ◽  
Asep Supriadin ◽  
Rani Maharani ◽  
Nurlelasari Nurlelasari ◽  
Tri Mayanti ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Leukemia Cell ◽  
2D Nmr ◽  
Leukemia Cell Lines ◽  
Ic50 Value ◽  
In Vitro Effects ◽  
Murine Leukemia Cell ◽  
Reported Data ◽  
Murine Leukemia

Four dammarane-type triterpenoids, dammardienon (1), aglaiabbreviatin E (2), dammar-20,25-dien-3b,24-diol (3) and dammar-24-en-3b,20-diol (4) were isolated from methanolic extract of the bark of Aglaia glabrata. The structures of all triterpenoids were elucidated by 1D-, 2D-NMR, and comparison with previously reported data. All triterpenoids were applied into in vitro bioassay against P-388 murine leukemia cell. Dammar-24-en-3b,20-diol (4) has cytotoxic activity with IC50 value of 9.45 mM towards P-388 murine leukemia cell lines.

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