scholarly journals Antiproliferative Activity of Triterpenoid and Steroid Compounds from Ethyl Acetate Extract of Calotropis gigantea Root Bark against P388 Murine Leukemia Cell Lines

2021 ◽  
Vol 89 (2) ◽  
pp. 21
Author(s):  
Kartini Hasballah ◽  
Murniana Sarong ◽  
Renzavaldy Rusly ◽  
Herdina Fitria ◽  
Dewi Rara Maida ◽  
...  

Calotropis gigantea has been known to produce bioactive secondary metabolites with antiproliferative activities against cancer cells. Herein, we extracted the secondary metabolites using ethyl acetate from its root bark and further tested its antiproliferative activities against P388 murine leukemia cell lines. The subfractions from the ethyl acetate extract was obtained from Vacuum Liquid Column Chromatography (VLCC), and followed by Gravity Column Chromatography (GCC). The subfraction C2 and D1 were identified to contain triterpenoids and steroids with the most potent cytotoxicity against Brine Shrimp Lethality Test (BSLT). A 3-(4,5-dimethylthiazol-2-yl) -2-5 diphenyl tetrazolium bromide (MTT) assay suggested that ethyl acetate extract has the highest antiproliferative activities against P388 murine leukemia cell lines (IC50 = 21.79 μg/mL), as opposed to subfraction C2 (IC50 = 50.64 µg/mL) and subfraction D1 (IC50 = 49.33 µg/mL). The compound identified in subfraction C2 and D1 are taraxerol acetate and calotropone, respectively. Though taraxerol acetate and calotropone were active in inhibiting the leukemic cell lines, their IC50s were lower than the ethyl acetate extract, which is probably due to the synergism of the secondary metabolites.

1999 ◽  
Vol 77 (1-2) ◽  
pp. 47-50 ◽  
Author(s):  
John D. Roberts ◽  
John Peroutka ◽  
G. Beggiolin ◽  
C. Manzotti ◽  
L. Piazzoni ◽  
...  

1988 ◽  
Vol 12 (9) ◽  
pp. 763-771 ◽  
Author(s):  
Hitoshi Sawada ◽  
Katsuhiko Itoh ◽  
Teruo Kirikae ◽  
Hiroto Sakoda ◽  
Hiroaki Tezuka ◽  
...  

2015 ◽  
Vol 50 (1) ◽  
pp. 33 ◽  
Author(s):  
Sol-Rim Jeon ◽  
Jae-Wook Lee ◽  
Pil-Sang Jang ◽  
Nack-Gyun Chung ◽  
Bin Cho ◽  
...  

Author(s):  
Kartini Hasballah ◽  
Murniana . ◽  
Erya . ◽  
Ardian .

<p><strong>Objective: </strong>The present study deals with the cytotoxic activity of n-hexane and ethyl acetate extracts of <em>Calotropis gigantea</em> L. stem bark and its fractions such as A, B, C, D and E fractions on murine leukemia cell line P388.</p><p><strong>Methods: </strong>The crude extracts of <em>C. gigantea</em> stem bark were prepared using n-hexane and ethyl acetate solvents. The plant extracts were subjected to vacuum liquid chromatography followed by TLC. According to the similarity of stain patterns, the fractions were combined. The extracts and its combined fractions were then subjected for the phytochemical test. Cytotoxic activity of those extracts and its combined fractions were tested using MTT assay. Fraction D was subjected to gravity column chromatography followed by TLC. Then, fractions A, B, and D2 were crystallized and subjected to GC-MS.</p><p><strong>Results: </strong>The qualitative screening of n-hexane extract of <em>Calotropis gigantea</em> L. stem bark for secondary metabolites showed the presence of terpenoid, flavonoids, phenolics and coumarins. While the ethyl acetate extract contained phenolics, steroids, flavonoids, saponins and coumarins compounds. IC<sub>50 </sub>values for n-hexane extract and E fraction are 76.29 µg/ml and 18.48 µg/ml, respectively. In the ethyl acetate extract and C fraction obtained IC<sub>50</sub> values 57.05 µg/ml and 52.58 µg/ml.</p><p><strong>Conclusion: </strong>Cytotoxic activity from E fraction of n-hexane extract of <em>C. gigantea</em> stem bark is the most potent and containing flavonoids, phenolics and coumarins. The main components from several compounds of n-hexane extract of <em>C. gigantea</em> are germacrane-A, (-)-globulol, urs-12-ene and veridiflorol. </p>


2019 ◽  
Vol 35 (1) ◽  
pp. 134-139
Author(s):  
Desi Harneti ◽  
Asep Supriadin ◽  
Rani Maharani ◽  
Nurlelasari Nurlelasari ◽  
Tri Mayanti ◽  
...  

Four dammarane-type triterpenoids, dammardienon (1), aglaiabbreviatin E (2), dammar-20,25-dien-3b,24-diol (3) and dammar-24-en-3b,20-diol (4) were isolated from methanolic extract of the bark of Aglaia glabrata. The structures of all triterpenoids were elucidated by 1D-, 2D-NMR, and comparison with previously reported data. All triterpenoids were applied into in vitro bioassay against P-388 murine leukemia cell. Dammar-24-en-3b,20-diol (4) has cytotoxic activity with IC50 value of 9.45 mM towards P-388 murine leukemia cell lines.


2020 ◽  
Author(s):  
Lokesh ST ◽  
Thippeswamy Basaiah ◽  
Sowmya HV ◽  
Vijaya Kumar ML

Abstract The present study deals with the secondary metabolites of Claviceps purpurea extract, which has been confirmed as an empirically potential cure for cancer. The purpose of this research was to prove scientifically the anticancer potential of ethyl acetate extract as its effects on the cell cycle, membrane apoptosis, DNA fragmentation and nuclear staining of lung cancer (A549) cell lines. The MTT method was used to measure cytotoxic effects, ethyl acetate extract of Claviceps purpurea showed IC50 value of 56.38 µg/ml compare to other cancer cell lines like breast cancer (MCF-7) cell lines (90.04 µg/ml) and cervical cancer (HeLa) cell lines (84.97 µg/ml) and normal cell lines fibroblast (3T3L1) does not show IC50 values due to lesser percentage of inhibition. The effect of inhibition of cell cycle and induction of cell apoptosis was measured by the flow cytometry method. DNA fragmentation studied by the gel electrophoresis method. The results showed, the ethyl acetate extract of Claviceps purpurea gives an induction of apoptosis effect on A549 cancer cell through inhibition of cell cycle in the G0-G1, Synthesis, and G2/M phases. In necrotic cell regions apoptosis was found. In DNA fragmentation 80 μg/ml concentration of extract showed significant results against A549 cell lines. In the nuclear staining study, membrane blebbing will be found. It indicates the extract was very active against cancer cell lines and it occurs in late apoptotic phase. In this study of all parameters, Colchicine was used as standard for cell cycle studies, for membrane apoptosis studies vinblastine and DNA fragmentation studies hydrogen peroxide was used.


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