scholarly journals Triterpenoids from the Bark of Aglaia glabrata and their In vitro Effects on P-388 Murine Leukemia Cells

2019 ◽  
Vol 35 (1) ◽  
pp. 134-139
Author(s):  
Desi Harneti ◽  
Asep Supriadin ◽  
Rani Maharani ◽  
Nurlelasari Nurlelasari ◽  
Tri Mayanti ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Leukemia Cell ◽  
2D Nmr ◽  
Leukemia Cell Lines ◽  
Ic50 Value ◽  
In Vitro Effects ◽  
Murine Leukemia Cell ◽  
Reported Data ◽  
Murine Leukemia

Four dammarane-type triterpenoids, dammardienon (1), aglaiabbreviatin E (2), dammar-20,25-dien-3b,24-diol (3) and dammar-24-en-3b,20-diol (4) were isolated from methanolic extract of the bark of Aglaia glabrata. The structures of all triterpenoids were elucidated by 1D-, 2D-NMR, and comparison with previously reported data. All triterpenoids were applied into in vitro bioassay against P-388 murine leukemia cell. Dammar-24-en-3b,20-diol (4) has cytotoxic activity with IC50 value of 9.45 mM towards P-388 murine leukemia cell lines.

scholarly journals Emodin Induces Apoptotic Death in Murine Myelomonocytic Leukemia WEHI-3 CellsIn Vitroand Enhances Phagocytosis in Leukemia MiceIn Vivo

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Yuan-Chang Chang ◽  
Tung-Yuan Lai ◽  
Chun-Shu Yu ◽  
Hung-Yi Chen ◽  
Jai-Sing Yang ◽  
...  
Keyword(s):  
Chromatin Condensation ◽  
Leukemia Cell ◽  
Cell Types ◽  
Leukemia Cell Line ◽  
Apoptotic Death ◽  
Murine Leukemia Cell ◽  
Available Information ◽  
Murine Leukemia

Emodin is one of major compounds in rhubarb (Rheum palmatumL.), a plant used as herbal medicine in Chinese population. Although many reports have shown that emodin exhibits anticancer activity in many tumor cell types, there is no available information addressing emodin-affected apoptotic responses in the murine leukemia cell line (WEHI-3) and modulation of the immune response in leukemia mice. We investigated that emodin induced cytotoxic effectsin vitroand affected WEHI-3 cellsin vivo. This study showed that emodin decreased viability and induced DNA fragmentation in WEHI-3 cells. Cells after exposure to emodin for 24 h have shown chromatin condensation and DNA damage. Emodin stimulated the productions of ROS and Ca2+and reduced the level ofΔΨmby flow cytometry. Our results from Western blotting suggest that emodin triggered apoptosis of WEHI-3 cells through the endoplasmic reticulum (ER) stress, caspase cascade-dependent and -independent mitochondrial pathways. Inin vivostudy, emodin enhanced the levels of B cells and monocytes, and it also reduced the weights of liver and spleen compared with leukemia mice. Emodin promoted phagocytic activity bymonocytesandmacrophagesin comparison to the leukemia mice group. In conclusions, emodin induced apoptotic death in murine leukemia WEHI-3 cells and enhanced phagocytosis in the leukemia animal model.

Comparison of cytotoxicity and cellular accumulation of polynuclear platinum complexes in L1210 murine leukemia cell lines

1999 ◽  
Vol 77 (1-2) ◽  
pp. 47-50 ◽  
Author(s):  
John D. Roberts ◽  
John Peroutka ◽  
G. Beggiolin ◽  
C. Manzotti ◽  
L. Piazzoni ◽  
...  
Keyword(s):  
Cell Lines ◽  
Leukemia Cell ◽  
Platinum Complexes ◽  
Cellular Accumulation ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Murine Leukemia

scholarly journals Antiproliferative Activity of Triterpenoid and Steroid Compounds from Ethyl Acetate Extract of Calotropis gigantea Root Bark against P388 Murine Leukemia Cell Lines

2021 ◽  
Vol 89 (2) ◽  
pp. 21
Author(s):  
Kartini Hasballah ◽  
Murniana Sarong ◽  
Renzavaldy Rusly ◽  
Herdina Fitria ◽  
Dewi Rara Maida ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Ethyl Acetate Extract ◽  
Leukemia Cell ◽  
Root Bark ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Murine Leukemia ◽  
Antiproliferative Activities

Calotropis gigantea has been known to produce bioactive secondary metabolites with antiproliferative activities against cancer cells. Herein, we extracted the secondary metabolites using ethyl acetate from its root bark and further tested its antiproliferative activities against P388 murine leukemia cell lines. The subfractions from the ethyl acetate extract was obtained from Vacuum Liquid Column Chromatography (VLCC), and followed by Gravity Column Chromatography (GCC). The subfraction C2 and D1 were identified to contain triterpenoids and steroids with the most potent cytotoxicity against Brine Shrimp Lethality Test (BSLT). A 3-(4,5-dimethylthiazol-2-yl) -2-5 diphenyl tetrazolium bromide (MTT) assay suggested that ethyl acetate extract has the highest antiproliferative activities against P388 murine leukemia cell lines (IC50 = 21.79 μg/mL), as opposed to subfraction C2 (IC50 = 50.64 µg/mL) and subfraction D1 (IC50 = 49.33 µg/mL). The compound identified in subfraction C2 and D1 are taraxerol acetate and calotropone, respectively. Though taraxerol acetate and calotropone were active in inhibiting the leukemic cell lines, their IC50s were lower than the ethyl acetate extract, which is probably due to the synergism of the secondary metabolites.

Establishment of a hemopoietic stimulating factor producing murine leukemia cell lines: Pathogenesis of granulocytosis in L8313 bearing mice

1988 ◽  
Vol 12 (9) ◽  
pp. 763-771 ◽  
Author(s):  
Hitoshi Sawada ◽  
Katsuhiko Itoh ◽  
Teruo Kirikae ◽  
Hiroto Sakoda ◽  
Hiroaki Tezuka ◽  
...  
Keyword(s):  
Cell Lines ◽  
Leukemia Cell ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Stimulating Factor ◽  
Murine Leukemia

Thyroid hormone responsiveness of the L1210 murine leukemia cell line

1992 ◽  
Vol 126 (4) ◽  
pp. 374-377 ◽  
Author(s):  
J Brtko ◽  
P Filipčík ◽  
J Knopp ◽  
V Sedláková ◽  
Keyword(s):  
Cell Cycle ◽  
Growth Rate ◽  
Protein Phosphorylation ◽  
Nuclear Receptors ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
L1210 Leukemia ◽  
Murine Leukemia Cell ◽  
Murine Leukemia

The presence of saturable and high affinity 3,5,3′-triiodothyronine (T3) binding sites was demonstrated in LI 210 murine leukemia cell nuclei. Scatchard analysis revealed one class of receptors for T3 with Ka = 2.187 × 109l/mol and a maximum binding capacity (Bmax) of 3.96 fmol/106 cells. The effects of T3 on protein phosphorylation and growth rate of L1210 cells were investigated in a medium containing T3-depleted fetal calf serum. T3 was observed to be effective in enhancing protein phosphorylation (153.06%±5.99 sd) compared to cells grown in the absence of T3 (81.49%±13.50 sd). Moreover, in the presence of high T3 concentration (11.15 nmol/l) T3 was found to significantly increase the cell growth rate. In addition, the T3 receptor-associated alterations during the cell cycle, as measured by flow cytometry, suggest that the presence of T3 receptors becomes evident during the late G3 phase of the cell cycle, and T3 receptor numbers increase during the S phase. These results suggest that in in vitro conditions representing high T3 concentration, the number of L1210 leukemia cells may be increased by T3 via nuclear receptors. The L1210 leukemia cell line may serve as a convenient tool for in vitro studies of nuclear receptors and/or mechanism of action of T3. The binding affinity of T3 receptors is similar to that found in rat hepatocytes or human lymphocytes.

scholarly journals Cyclic Polyketides with α-Glucosidase Inhibitory Activity from Endiandra kingiana Gamble and Molecular Docking Study

2021 ◽  
Vol 15 (5) ◽  
pp. 414-419
Author(s):  
Mohamad Nurul Azmi Mohamad Taib ◽  
Nur Amirah Saad ◽  
Mohamad Hafizi Abu Bakar ◽  
Mohammad Tasyriq Che Omar ◽  
Ahmad Nazif Aziz ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Active Site ◽  
Methanolic Extract ◽  
Docking Study ◽  
2D Nmr ◽  
Inhibition Activity ◽  
Molecular Docking Study ◽  
Phytochemical Investigation ◽  
Ic50 Value

A phytochemical investigation of the methanolic extract of Endiandra kingiana (bark) led to the isolation of four major compounds which are kingianic acid A (1), tsangibeilin B (2), kingianin A (3) and kingianin F (4). The structures were determined by 1D- and 2D-NMR analysis in combination with HRMS experiments. The compounds were screened for their in vitro α-glucosidase inhibition activity. Among them, compounds 3-4 showed potent α-glucosidase inhibition activity with IC50 value at 11.9 ± 2.0 µM and 19.7 ± 1.5 µM, respectively. The molecular docking study found that both compounds were bound into the active site of the N-terminal of MGAM, and thus agreed with the in vitro α-glucosidase enzyme inhibition activity results.

scholarly journals Anti-leukemic properties of deferasirox via apoptosis in murine leukemia cell lines

2015 ◽  
Vol 50 (1) ◽  
pp. 33 ◽  
Author(s):  
Sol-Rim Jeon ◽  
Jae-Wook Lee ◽  
Pil-Sang Jang ◽  
Nack-Gyun Chung ◽  
Bin Cho ◽  
...  
Keyword(s):  
Cell Lines ◽  
Leukemia Cell ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Murine Leukemia
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