Anti-leukemic properties of deferasirox via apoptosis in murine leukemia cell lines

Calotropis gigantea has been known to produce bioactive secondary metabolites with antiproliferative activities against cancer cells. Herein, we extracted the secondary metabolites using ethyl acetate from its root bark and further tested its antiproliferative activities against P388 murine leukemia cell lines. The subfractions from the ethyl acetate extract was obtained from Vacuum Liquid Column Chromatography (VLCC), and followed by Gravity Column Chromatography (GCC). The subfraction C2 and D1 were identified to contain triterpenoids and steroids with the most potent cytotoxicity against Brine Shrimp Lethality Test (BSLT). A 3-(4,5-dimethylthiazol-2-yl) -2-5 diphenyl tetrazolium bromide (MTT) assay suggested that ethyl acetate extract has the highest antiproliferative activities against P388 murine leukemia cell lines (IC50 = 21.79 μg/mL), as opposed to subfraction C2 (IC50 = 50.64 µg/mL) and subfraction D1 (IC50 = 49.33 µg/mL). The compound identified in subfraction C2 and D1 are taraxerol acetate and calotropone, respectively. Though taraxerol acetate and calotropone were active in inhibiting the leukemic cell lines, their IC50s were lower than the ethyl acetate extract, which is probably due to the synergism of the secondary metabolites.

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Establishment of a hemopoietic stimulating factor producing murine leukemia cell lines: Pathogenesis of granulocytosis in L8313 bearing mice

Leukemia Research ◽

10.1016/0145-2126(88)90010-0 ◽

1988 ◽

Vol 12 (9) ◽

pp. 763-771 ◽

Cited By ~ 10

Author(s):

Hitoshi Sawada ◽

Katsuhiko Itoh ◽

Teruo Kirikae ◽

Hiroto Sakoda ◽

Hiroaki Tezuka ◽

...

Keyword(s):

Cell Lines ◽

Leukemia Cell ◽

Leukemia Cell Lines ◽

Murine Leukemia Cell ◽

Stimulating Factor ◽

Murine Leukemia

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Molecular Analysis of Murine Leukemia Cell Lines Resistant to 5,10-Dideazatetrahydrofolate Identifies Several Amino Acids Critical to the Function of Folylpolyglutamate Synthetase

Journal of Biological Chemistry ◽

10.1074/jbc.m002580200 ◽

2000 ◽

Vol 275 (34) ◽

pp. 26599-26606 ◽

Cited By ~ 27

Author(s):

Rongbao Zhao ◽

Steven Titus ◽

Feng Gao ◽

Richard G. Moran ◽

I. David Goldman

Keyword(s):

Amino Acids ◽

Cell Lines ◽

Molecular Analysis ◽

Leukemia Cell ◽

Folylpolyglutamate Synthetase ◽

Leukemia Cell Lines ◽

Murine Leukemia Cell ◽

Murine Leukemia

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Triterpenoids from the Bark of Aglaia glabrata and their In vitro Effects on P-388 Murine Leukemia Cells

Oriental Journal Of Chemistry ◽

10.13005/ojc/350114 ◽

2019 ◽

Vol 35 (1) ◽

pp. 134-139

Author(s):

Desi Harneti ◽

Asep Supriadin ◽

Rani Maharani ◽

Nurlelasari Nurlelasari ◽

Tri Mayanti ◽

...

Keyword(s):

Methanolic Extract ◽

Leukemia Cell ◽

2D Nmr ◽

Leukemia Cell Lines ◽

Ic50 Value ◽

In Vitro Effects ◽

Murine Leukemia Cell ◽

Reported Data ◽

Murine Leukemia

Four dammarane-type triterpenoids, dammardienon (1), aglaiabbreviatin E (2), dammar-20,25-dien-3b,24-diol (3) and dammar-24-en-3b,20-diol (4) were isolated from methanolic extract of the bark of Aglaia glabrata. The structures of all triterpenoids were elucidated by 1D-, 2D-NMR, and comparison with previously reported data. All triterpenoids were applied into in vitro bioassay against P-388 murine leukemia cell. Dammar-24-en-3b,20-diol (4) has cytotoxic activity with IC50 value of 9.45 mM towards P-388 murine leukemia cell lines.

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