Resistance to stripe rust in wheat: pathogen biology driving resistance breeding.

Abstract Background: Lesion mimics (LMs) are disease-like symptoms that occur randomly on plant green leaves in the absence of pathogens. A previous study showed that LMs are related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can substantially reduce the quality and yield of crops. The development of resistant cultivars is an economical and environmentally friendly way to enhance the adaptability and yield stability of crops instead of the use of fungicide applications. Results: In this study, a novel LM gene affording Pst resistance was identified and mapped with molecular markers developed for marker-assisted selection (MAS)-based wheat breeding. In this study, a novel LM gene named lm4, which is closely linked (8.06 cM) to SSR markers Xgwm210 and Xgwm455, was identified by using a Yanzhan 1/Neixiang 188 RIL population. The genetic distance of lm4 was then narrowed such that it was flanked by SSR markers with 0.51 cM and 0.77 cM intervals. Two SSR markers, lm4_01_cib and lm4_02_cib, were developed based on the content in the Chinese Spring genome database and wheat 660K SNP results; these markers can be used to conduct MAS of LMs in wheat. The results also showed that lm4 significantly improved the resistance of stripe rust in wheat. Conclusions: Therefore, lm4 is associated with stripe rust resistance, which may provide theoretical support for future crop disease-resistance breeding and for understanding the plant apoptosis mechanism.

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Molecular identification and mapping of a novel stripe rust resistance gene in wheat resistance line CH5389

Ciência Rural ◽

10.1590/0103-8478cr20170846 ◽

2018 ◽

Vol 48 (5) ◽

Author(s):

Haixian Zhan ◽

Huijuan Guo ◽

Linyi Qiao ◽

Liping Mao ◽

Shuosheng Zhang

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Dominant Gene ◽

Genomic Analysis ◽

Resistance Breeding ◽

Genetic Distances ◽

Stripe Rust Resistance ◽

Comparative Genomic

ABSTRACT: Stripe rust, caused by Puccinia striiformis is one of the most destructive diseases of wheat worldwide. CH5389 is a wheat-Thinopyrum intermedium derived line conferring stripe rust resistance. Genetic analyses of seedlings of F2 populations and F2:3 families developed by crossing CH5389 and susceptible common wheat revealed that stripe rust resistance in CH5389 was controlled by a single dominant gene that was designated YrCH5389. Eight SSR and EST-PCR polymorphic markers on chromosome 3AL were identified in F2 population of CH5389/Taichung29. The YrCH5389 was flanked by EST marker BE405348 and SSR marker Xwmc388 on chromosome 3AL with genetic distances of 2.2 and 4.6 cM, respectively. Comparative genomic analysis demonstrated that the orthologous genomic region of YrCH5389 covered 990 kb in rice, 640 kb in Brachypodium, and 890 kb in sorghum. Based on the locations of the markers, the resistance gene was located to chromosome deletion bin 3AL-0.85-1.00. Because there are no officially named stripe rust resistance genes on the 3AL chromosome, the YrCH5389 should be designated as a new resistance gene. These linkage markers could be useful for marker-assisted selection in wheat resistance breeding.

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Wheat stripe rust in China

Australian Journal of Agricultural Research ◽

10.1071/ar06142 ◽

2007 ◽

Vol 58 (6) ◽

pp. 605 ◽

Cited By ~ 149

Author(s):

A. M. Wan ◽

X. M. Chen ◽

Z. H. He

Keyword(s):

Stripe Rust ◽

Resistance Breeding ◽

Agricultural Practice ◽

Wheat Stripe Rust ◽

North West ◽

Western Sichuan ◽

The North ◽

Pathogen Variability ◽

Application Diversification ◽

Chinese Wheat

China has the largest stripe rust epidemic region in the world in terms of wheat acreage affected by the disease. Extensive studies on the epidemiology and management of stripe rust have been carried out since the widespread occurrence of the disease in the 1950s. Fifteen epidemic zones were classified, based on factors influencing the occurrence of wheat stripe rust. The disease can spread inter-regionally, mainly from west to east and from south to north. Winter-wheat growing regions in the north-west, south-west, and north, and spring-wheat growing regions in the north-west are the major epidemic areas. Hotspots of wheat stripe rust are mainly located in south-eastern Gansu and north-western Sichuan, and these areas constitute the inoculum base, centre of diversity, and major over-summering areas of the pathogen. Successful control in over-summering areas is the key to achieving sustainable management of stripe rust in China. Pathogen variability and race virulence have been monitored continuously, and the resistance genetics and background of Chinese wheat cultivars analysed. Effective management of stripe rust includes resistance breeding and application, diversification and deployment of various resistance genes, use of chemicals, adoption of appropriate agricultural practice, and integrated disease management in ‘hotspot’ regions. Collaboration of scientists among various organisations and disciplines is vital. We discuss the progress and challenges of wheat stripe rust management in China.

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Molecular mapping of a novel lesion mimic gene (lm4) associated with enhanced resistance to stripe rust in bread wheat

BMC Genomic Data ◽

10.1186/s12863-021-00963-6 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Rong Liu ◽

Jing Lu ◽

Shigang Zheng ◽

Mei Du ◽

Chihong Zhang ◽

...

Keyword(s):

Ssr Markers ◽

Stripe Rust ◽

Molecular Mapping ◽

Resistance Breeding ◽

Wheat Breeding ◽

Stripe Rust Resistance ◽

Genome Database ◽

Theoretical Support ◽

Enhanced Resistance ◽

Crop Disease

Abstract Background Lesion mimics (LMs) are disease-like symptoms that occur randomly on plant green leaves in the absence of pathogens. A previous study showed that LMs are related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can substantially reduce the quality and yield of crops. The development of resistant cultivars is an economical and environmentally friendly way to enhance the adaptability and yield stability of crops instead of the use of fungicide applications. Results In this study, a novel LM gene affording Pst resistance was identified and mapped with molecular markers developed for marker-assisted selection (MAS)-based wheat breeding. In this study, a novel LM gene named lm4, which is closely linked (8.06 cM) to SSR markers Xgwm210 and Xgwm455, was identified by using a Yanzhan 1/Neixiang 188 RIL population. The genetic distance of lm4 was then narrowed such that it was flanked by SSR markers with 0.51 cM and 0.77 cM intervals. Two SSR markers, lm4_01_cib and lm4_02_cib, were developed based on the content in the Chinese Spring genome database and wheat 660 K SNP results; these markers can be used to conduct MAS of LMs in wheat. The results also showed that lm4 significantly improved the resistance of stripe rust in wheat. Conclusions Therefore, lm4 is associated with stripe rust resistance, which may provide theoretical support for future crop disease-resistance breeding and for understanding the plant apoptosis mechanism.

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Identification of temperature sensitive resistance to Puccinia striiformis f. sp. tritici in wheat differential hosts and its potential applications in resistance breeding

10.36811/ijpsh.2019.110015 ◽

2019 ◽

pp. 163-172

Author(s):

ING FENG ◽

HAITING ZENG ◽

FENGTAO WANG ◽

RUIMING LIN

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Infection Type ◽

Resistance Breeding ◽

Temperature Sensitive ◽

Wheat Cultivars ◽

Temperature Regimes ◽

Potential Applications ◽

Differential Hosts ◽

Two Temperature

Temperature affects wheat resistance responses infected by Puccinia striiformis f. sp. tritici (Pst). In order to identify if thirty-one entries of Chinese, international and other tester wheat cultivars possess temperature sensitive resistance, the entries were studied in seedling stage at two different day/night temperature regimes (24/18°C and 14/10°C). Four entries, Lutescens 128, Funo, Lee and Carstens V, were confirmed no temperature sensitive resistance genes. Six wheat cultivars, Early Piemium, Fengchan 3, Fulhard, Heines VII, Mentana and Virgilio, have shown temperature sensitive resistance. Comparison with four standard lines (S110, S111, S112, S113) with 0-3 temperature sensitive genes, derived from crosses of Itana/PI 178383, the resistance to Pst race 10E162 in Virgilio controlled by two temperature sensitive genes, and Mentana and Fulhard each possessed one temperature sensitive gene. Virgilio, Fulhard and Mentana as the temperature sensitive gene resources are useful in breeding for resistance to stripe rust. As the differential hosts of wheat stripe rust, it is necessary to strictly control the temperature without exceeding 18°C, since infection type may differ due to the different temperature. Keywords: Differential hosts; Puccinia striiformis f. sp. tritici; Temperature sensitive resistance; Wheat

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