Evaluating the contribution of Yr genes to stripe rust resistance breeding through marker-assisted detection in wheat

Stripe rust, caused by Puccinia striiormis Westend f. sp. tritici, is one of the most important foliar diseases of wheat ( Triticum aestivum L.) worldwide. Stripe rust resistance genes Yr27, Yr31, YrSp, YrV23, and YrCN19 on chromosome 2BS confer resistance to some or all Chinese P. striiormis f. sp. tritici races CYR31, CYR32, SY11-4, and SY11-14 in the greenhouse. To screen microsatellite (SSR) markers linked with YrCN19, F1, F2, and F3 populations derived from cross Ch377/CN19 were screened with race CYR32 and 35 SSR primer pairs. Linkage analysis indicated that the single dominant gene YrCN19 in cultivar CN19 was linked with SSR markers Xgwm410, Xgwm374, Xwmc477, and Xgwm382 on chromosome 2BS with genetic distances of 0.3, 7.9, 12.3, and 21.2 cM, respectively. Crosses of CN19 with wheat lines carrying other genes on chromosome 2B showed that all were located at different loci. YrCN19 is thus different from the other reported Yr genes in chromosomal location and resistance response and was therefore named Yr41. Prospects and strategies of using Yr41 and other Yr genes in wheat improvement for stripe rust resistance are discussed.

Download Full-text

Molecular identification and mapping of a novel stripe rust resistance gene in wheat resistance line CH5389

Ciência Rural ◽

10.1590/0103-8478cr20170846 ◽

2018 ◽

Vol 48 (5) ◽

Author(s):

Haixian Zhan ◽

Huijuan Guo ◽

Linyi Qiao ◽

Liping Mao ◽

Shuosheng Zhang

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Dominant Gene ◽

Genomic Analysis ◽

Resistance Breeding ◽

Genetic Distances ◽

Stripe Rust Resistance ◽

Comparative Genomic

ABSTRACT: Stripe rust, caused by Puccinia striiformis is one of the most destructive diseases of wheat worldwide. CH5389 is a wheat-Thinopyrum intermedium derived line conferring stripe rust resistance. Genetic analyses of seedlings of F2 populations and F2:3 families developed by crossing CH5389 and susceptible common wheat revealed that stripe rust resistance in CH5389 was controlled by a single dominant gene that was designated YrCH5389. Eight SSR and EST-PCR polymorphic markers on chromosome 3AL were identified in F2 population of CH5389/Taichung29. The YrCH5389 was flanked by EST marker BE405348 and SSR marker Xwmc388 on chromosome 3AL with genetic distances of 2.2 and 4.6 cM, respectively. Comparative genomic analysis demonstrated that the orthologous genomic region of YrCH5389 covered 990 kb in rice, 640 kb in Brachypodium, and 890 kb in sorghum. Based on the locations of the markers, the resistance gene was located to chromosome deletion bin 3AL-0.85-1.00. Because there are no officially named stripe rust resistance genes on the 3AL chromosome, the YrCH5389 should be designated as a new resistance gene. These linkage markers could be useful for marker-assisted selection in wheat resistance breeding.

Download Full-text

Association Analysis Identifies New Loci for Resistance to Chinese Yr26-Virulent Races of the Stripe Rust Pathogen in a Diverse Panel of Wheat Germplasm

Plant Disease ◽

10.1094/pdis-12-19-2663-re ◽

2020 ◽

Vol 104 (6) ◽

pp. 1751-1762 ◽

Cited By ~ 3

Author(s):

Jianhui Wu ◽

Xiaoting Wang ◽

Nan Chen ◽

Rui Yu ◽

Shizhou Yu ◽

...

Keyword(s):

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Snp Array ◽

Stripe Rust Resistance ◽

Phenotypic Effect ◽

Breeding Programs ◽

Stripe Rust Resistance Gene ◽

Trait Locus ◽

Yr Genes

Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is one of the most destructive fungal diseases of wheat worldwide. The expanding Yr26-virulent Pst race (V26) group overcomes almost all currently deployed resistance genes in China and has continued to accumulate new virulence. Investigating the genetic architecture of stripe rust resistance in common wheat is an important basis for a successful utilization of resistance in breeding programs. A panel of 410 exotic wheat germplasms was used for characterizing new stripe rust resistance loci. This panel was genotyped using high-density wheat 660K single-nucleotide polymorphism (SNP) array, and phenotypic evaluation of seedlings for stripe rust resistance was performed using multiple Pst races. Thirty-five loci conferring resistance were identified through genome-wide association mapping, and explained phenotypic variances ranged from 53 to 75%. Of these, 14 were colocated in the proximity of the known loci, including cataloged Yr genes Yr9, Yr10, Yr26, Yr33, Yr47, Yr56, Yr57, Yr64, Yr67, Yr72, and Yr81 and three temporarily designated as YrCen, YrNP63, and YrRC detected in our quantitative trait locus (QTL) mapping studies. Seven of them (Yr9, Yr10, Yr24/26, Yr81, YrCEN, YrNP63, and YrRC) were confirmed by molecular detection or genetic analysis. New loci that were identified to be different from reported Yr genes need further confirmation. Nine QTL with significantly large phenotypic effect on resistance to all tested races were considered as major loci for effective resistance. The identified loci enrich our stripe rust resistance gene pool, and the linked SNPs should be useful for marker-assisted selection in breeding programs.

Download Full-text

Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars From the Huang-Huai-Hai Region of China

Plant Disease ◽

10.1094/pdis-10-19-2119-re ◽

2020 ◽

Vol 104 (6) ◽

pp. 1763-1770

Author(s):

Liang Huang ◽

Xing Zhi Xiao ◽

Bo Liu ◽

Li Gao ◽

Guo Shu Gong ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Molecular Testing ◽

Wheat Cultivars ◽

Rust Resistance Genes ◽

Yr Genes

Wheat stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious fungal disease worldwide, especially in the Huang-Huai-Hai region, a main wheat production area in China. Gene postulation, molecular testing, and pedigree analysis were conducted to determine the presence of stripe rust resistance genes to 15 Pst races in 66 selected commercial wheat cultivars released from 2000 to 2016. In addition, races CYR32, CYR33, and CYR34 were used to evaluate resistance to Pst at the adult-plant stage of wheat in the field. Four Yr genes (Yr9, Yr10, Yr26, and Yr32) were postulated in 24 wheat cultivars either singly or in combination. Thirty-six cultivars might contain unknown Yr genes, whereas no identified Yr gene was postulated in six cultivars. Yr9 was detected at a frequency of 28.8%, and no cultivars carried Yr5, Yr15, or Yr18. Ten cultivars (15.2%) exhibited adult-plant resistance in the field tests with three predominant races. Three cultivars (Langyan 43, Xinong 889, and Yunfeng 139) had all-stage resistance. These results are useful to growers selecting cultivars and to breeders aiming to use more resistance genes to develop new cultivars with effective resistance in order to reduce stripe rust damage.

Download Full-text

Advances in identifying stripe rust resistance genes in cereals

10.19103/as.2021.0092.04 ◽

2021 ◽

pp. 39-80

Author(s):

Tianheng Ren ◽

◽

Zhi Li ◽

Feiquan Tan ◽

Cheng Jiang ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Wheat Breeding ◽

Stripe Rust Resistance ◽

Wheat Varieties ◽

Wheat Diseases ◽

The World ◽

Rust Resistance Genes ◽

Yr Genes

Stripe rust is one of the most serious wheat diseases of the world, usually resulting in massive loss of grain production. The most effective and environmentally friendly way to control the spread of stripe rust is to plant wheat varieties that carry stripe rust resistance genes. The identification and utilization of stripe rust resistance genes is very important for achieving this goal. This chapter summarizes the hazards of stripe rust and the current progress in the discovery of stripe rust resistance genes. It also introduces the advanced methods to identify Yr genes. The chapter also shows the successful application of Yr genes in wheat breeding program in southwestern China, which is the largest epidemic area of stripe rust in the world. The further identification and applications of Yr genes are also discussed.

Download Full-text