Dickeya solani (black leg disease of potato).

2021 ◽  
Author(s):  
Gerry Saddler
Keyword(s):  
Bacterial Pathogen ◽  
Potato Production ◽  
Northern Europe ◽  
Varietal Resistance ◽  
Seed Certification ◽  
Horticultural Crops ◽  
System P ◽  
Recent Emergence ◽  
Dickeya Solani ◽  
And Control

Abstract Dickeya solani is a bacterial pathogen of potato thought to have crossed from horticultural crops in Northern Europe in 2005-2006. The earliest reports of disease on potato are from Belgium and the Netherlands, though it is now present in most European countries and Israel. Symptoms range from blackleg to top wilt in the growing plant and soft rotting of tubers. Symptoms are indistinguishable from those caused by Pectobacterium atrosepticum, P. carotovorum, P. parmentieri (formerly P. wasabiae) (Khayi et al., 2016) and D. dianthicola and control is usually reliant on seed certification schemes to mitigate its worst effects. It should be noted that most losses are attributable to the certification process itself though losses as high as 30% have been recorded in crops grown in Israel. There is no evidence of varietal resistance in potato. D. solani is a highly clonal organism highlighting its recent emergence as a pathogen but also the vulnerability of Europe's highly integrated potato production system.

scholarly journals First Report of Dickeya solani Causing Soft Rot in Imported Bulbs of Hyacinthus orientalis in China

Plant Disease ◽  
2015 ◽  
Vol 99 (1) ◽  
pp. 155-155 ◽  
Author(s):  
X. F. Chen ◽  
H. L. Zhang ◽  
J. Chen
Keyword(s):  
16S Rrna ◽  
The Netherlands ◽  
Bacterial Pathogen ◽  
Soft Rot ◽  
Potato Production ◽  
Sterile Water ◽  
First Report ◽  
Hyacinthus Orientalis ◽  
Dickeya Solani ◽  
Negative Controls

A bacterial pathogen, Dickeya solani, emerged as a major threat to potato (Solanum tuberosum) production in Europe in 2004 and has spread to many potato-growing regions via international trade. In December 2013, soft rot symptoms were observed in hyacinth (Hyacinthus orientalis) bulbs imported from the Netherlands into China at Ningbo Port. Diseased bulbs gave off an offensive odor. The base and internal parts of diseased bulbs rotted, and the margins of diseased tissues showed brown discoloration. Isolation on nutrient agar glucose (NAG) medium resulted in dominating colonies of characteristic “fried egg” morphology (1). One colony was chosen for further investigation and tentatively named “isolate 6165-3.” Under microscopic visualization after gram stain, the cells of isolate 6165-3 were gram-negative, motile, and rod shaped. The isolate was then identified as a member of genus Dickeya using the Biolog GN microplate. The 16S rRNA, recA, and dnaX sequences of isolate 6165-3 were subsequently determined and deposited in GenBank with accession numbers KM405240, KM405241, and KM405242, sharing 99% (16S rRNA), 100% (recA), and 100% (dnaX) nucleotide identity with those of known D. solani isolates, respectively. By this means, the isolate 6165-3 was identified as D. solani (1,2). To confirm the pathogenicity of the isolate, four plants each of 30-day-old hyacinth, 14-day-old potato, and 60-day-old moth orchid (Phalaenopsis amabilis) were inoculated with suspensions of the isolate with a concentration of 108 CFU/ml in sterile water by stabbing. Plants were incubated in a climate chamber at 28°C during the day and 24°C during the night with a relative humidity of 93% and a photoperiod of 12/12 h. Plants inoculated with sterile water were included as negative controls. After 2 or 3 days, typical symptoms such as water-soaked lesions and soft rot developed around the inoculation point, while the negative controls remained symptomless. Koch's postulates were fulfilled by re-isolating bacteria from lesions, which had identical sequence and morphology characters with the inoculated isolate. This is the first report of intercepted D. solani on hyacinth bulbs imported from the Netherlands into China, indicating that D. solani can spread via hyacinth. Further spread of the pathogen into potato production might lead to immeasurable economic consequences for China. References: (1) P. F. Sarris et al. New Dis. Rep. 24:21, 2011. (2) J. M. van der Wolf et al. Int. J. Syst. Evol. Microbiol. 64:768, 2014.

scholarly journals Pectobacterium brasiliense (soft rot and blackleg of ornamentals and potato).

2021 ◽  
Author(s):  
Janak Raj Joshi ◽  
Yuan Zeng ◽  
Amy Charkowski
Keyword(s):  
South Africa ◽  
Bacterial Pathogen ◽  
Soft Rot ◽  
Varietal Resistance ◽  
Seed Certification ◽  
Wide Range ◽  
Cultivated Potatoes ◽  
North And South America ◽  
First Time ◽  
North And South

Abstract Pectobacterium brasiliense is a bacterial pathogen primarily infecting potato and other vegetables and ornamentals. The earliest reports of the bacterium causing disease were from Brazil in 2004 (El-Tassa and Duarte, 2004; Duarte et al., 2004). This pathogen was reported for the first time in Europe (Belgium) in 2012 (van der Wolf et al., 2017). Since then, the pathogen has been reported in many regions of Europe, Asia, Africa, Australia, North and South America. The bacterium has adapted itself to a wide range of temperatures and host species, thus it is considered a culprit for significant losses in China, South Africa, Brazil, Netherlands, Switzerland and UK (Meng et al., 2017; van der Wolf et al., 2017). Symptoms caused by P. brasiliense are indistinguishable from other soft rot Pectobacterium and Dickeya, therefore it is impossible to identify this species on the basis of field and laboratory symptoms. Unfortunately, there is no evidence of curative measures and varietal resistance (in cultivated potatoes) against this group of bacteria, so farmers rely on seed certification, exclusion and sanitation to mitigate its worst effects.

Pectobacterium brasiliense (soft rot and blackleg of ornamentals and potato).

2021 ◽  
Author(s):  
Janak Raj Joshi ◽  
Yuan Zeng ◽  
Amy Charkowski
Keyword(s):  
South Africa ◽  
Bacterial Pathogen ◽  
Soft Rot ◽  
Varietal Resistance ◽  
Seed Certification ◽  
Wide Range ◽  
Cultivated Potatoes ◽  
North And South America ◽  
First Time ◽  
North And South

Abstract Pectobacterium brasiliense is a bacterial pathogen primarily of potato and other vegetables and ornamentals. The earliest reports of the bacterium causing disease were from Brazil in 2004 (El-Tassa and Duarte, 2004). This pathogen was reported for the first time in Europe (Belgium) in 2012 (van der Wolf et al., 2017). Since then, the pathogen has been reported in many regions of Europe, Asia, Africa, Australia, North and South America. The bacterium has adapted itself to a wide range of temperatures and host species, thus it is considered a culprit for significant losses in China, South Africa, Brazil, Netherlands, Switzerland and UK (Meng et al., 2017; van der Wolf et al., 2017). Symptoms caused by P. brasiliense are indistinguishable from other soft rot Pectobacterium and Dickeya, therefore it is impossible to identify this species on the basis of field and laboratory symptoms. Unfortunately, there is no evidence of curative measures and varietal resistance (in cultivated potatoes) against this group of bacteria, so farmers rely on seed certification, exclusion and sanitation to mitigate its worst effects.

Autonomous Vehicles in the Mobility System

2020 ◽  
pp. 500-514
Author(s):  
Thilo von Pape
Keyword(s):  
Artificial Intelligence ◽  
Mobile Phones ◽  
Mobile Communication ◽  
Autonomous Vehicles ◽  
Communication Research ◽  
Collective Control ◽  
System P ◽  
Mobility System ◽  
And Control ◽  
Transportation Technologies

This chapter discusses how autonomous vehicles (AVs) may interact with our evolving mobility system and what they mean for mobile communication research. It juxtaposes a conceptualization of AVs as manifestations of automation and artificial intelligence with an analysis of our mobility system as a historically grown hybrid of communication and transportation technologies. Since the emergence of railroad and telegraph, this system has evolved on two layers: an underlying infrastructure to power and coordinate the movements of objects, people, and ideas in industrially scaled speeds, volumes, and complexity and an interface to seamlessly access this infrastructure and control it. AVs are poised to further enhance the seamlessness which mobile phones and cars already lent to mobility. But in assuming increasingly sophisticated control tasks, AVs also disrupt an established shift toward individual control, demanding new interfaces to enable higher levels of individual and collective control over the mobility infrastructure.

scholarly journals Unlocking the Potential of 46 New Bacteriophages for Biocontrol of Dickeya Solani

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 621 ◽  
Author(s):  
Alexander Carstens ◽  
Amaru Djurhuus ◽  
Witold Kot ◽  
Deborah Jacobs-Sera ◽  
Graham Hatfull ◽  
...  
Keyword(s):  
Soft Rot ◽  
Potato Production ◽  
Storage Conditions ◽  
Bacterial Diseases ◽  
Modern Agriculture ◽  
Production Pipeline ◽  
Global Demand ◽  
Phage Cocktail ◽  
Dickeya Solani ◽  
Sustainability Standards

Modern agriculture is expected to face an increasing global demand for food while also needing to comply with higher sustainability standards. Therefore, control of crop pathogens requires new, green alternatives to current methods. Potatoes are susceptible to several bacterial diseases, with infections by soft rot Enterobacteriaceae (SRE) being a significant contributor to the major annual losses. As there are currently no efficient ways of combating SRE, we sought to develop an approach that could easily be incorporated into the potato production pipeline. To this end, 46 phages infecting the emerging potato pathogen Dickeya solani were isolated and thoroughly characterized. The 46 isolated phages were grouped into three different groups based on DNA similarity, representing two distinct clusters and a singleton. One cluster showed similarity to phages previously used to successfully treat soft rot in potatoes, whereas the remaining phages were novel and showed only very limited similarity to previously isolated phages. We selected six diverse phages in order to create the hereto most complex phage cocktail against SRE. The cocktail was applied in a proof-of-principle experiment to treat soft rot in potatoes under simulated storage conditions. We show that the phage cocktail was able to significantly reduce the incidence of soft rot as well as disease severity after 5 days of storage post-infection with Dickeya solani. This confirms results from previous studies that phages represent promising biocontrol agents against SRE infection in potato.

scholarly journals Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani

2020 ◽  
Author(s):  
Agata Motyka-Pomagruk ◽  
Sabina Zoledowska ◽  
Agnieszka Emilia Misztak ◽  
Wojciech Sledz ◽  
Alessio Mengoni ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Large Scale ◽  
De Novo ◽  
Genetic Material ◽  
Soft Rot ◽  
Potato Production ◽  
Core Gene ◽  
Ecological Niches ◽  
Dickeya Solani ◽  
High Level

Abstract Background: Dickeya solani is an important plant pathogenic bacterium causing severe losses in European potato production. This species draws a lot of attention due to its remarkable virulence, great devastating potential and easier spread in contrast to other Dickeya spp. In view of a high need for extensive studies on economically important soft rot Pectobacteriaceae , we performed a comparative genomics analysis on D. solani strains to search for genetic foundations that would explain the differences in the observed virulence levels within the D. solani population. Results: High quality assemblies of 8 de novo sequenced D. solani genomes have been obtained. Whole-sequence comparison, ANIb, ANIm, Tetra and pangenome-oriented analyses performed on these genomes and the sequences of 14 additional strains revealed an exceptionally high level of homogeneity among the studied genetic material of D. solani strains. With the use of 22 genomes, the pangenome of D. solani , comprising 84.7% core, 7.2% accessory and 8.1% unique genes, has been almost completely determined, suggesting the presence of a nearly closed pangenome structure. Attribution of the genes included in the D. solani pangenome fractions to functional COG categories showed that higher percentages of accessory and unique pangenome parts in contrast to the core section are encountered in phage/mobile elements- and transcription- associated groups with the genome of RNS 05.1.2A strain having the most significant impact. Also, the first D. solani large-scale genome-wide phylogeny computed on concatenated core gene alignments is herein reported. Conclusions: The almost closed status of D. solani pangenome achieved in this work points to the fact that the unique gene pool of this species should no longer expand. Such a feature is characteristic of taxa whose representatives either occupy isolated ecological niches or lack efficient mechanisms for gene exchange and recombination, which seems rational concerning a strictly pathogenic species with clonal population structure. Finally, no obvious correlations between the geographical origin of D. solani strains and their phylogeny were found, which might reflect the specificity of the international seed potato market.

scholarly journals The S-layer protein of a Clostridium difficile SLCT-11 strain displays a complex glycan required for normal cell growth and morphology

2018 ◽  
Vol 293 (47) ◽  
pp. 18123-18137 ◽  
Author(s):  
Emma Richards ◽  
Laura Bouché ◽  
Maria Panico ◽  
Ana Arbeloa ◽  
Evgeny Vinogradov ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Biofilm Formation ◽  
Bacterial Pathogen ◽  
Low Molecular Weight ◽  
Major Health ◽  
Archaeal Species ◽  
Core Peptide ◽  
And Control ◽  
Protein Attachment ◽  
Complex Glycan

Clostridium difficile is a bacterial pathogen that causes major health challenges worldwide. It has a well-characterized surface (S)-layer, a para-crystalline proteinaceous layer surrounding the cell wall. In many bacterial and archaeal species, the S-layer is glycosylated, but no such modifications have been demonstrated in C. difficile. Here, we show that a C. difficile strain of S-layer cassette type 11, Ox247, has a complex glycan attached via an O-linkage to Thr-38 of the S-layer low-molecular-weight subunit. Using MS and NMR, we fully characterized this glycan. We present evidence that it is composed of three domains: (i) a core peptide–linked tetrasaccharide with the sequence -4-α-Rha-3-α-Rha-3-α-Rha-3-β-Gal-peptide; (ii) a repeating pentasaccharide with the sequence -4-β-Rha-4-α-Glc-3-β-Rha-4-(α-Rib-3-)β-Rha-; and (iii) a nonreducing end–terminal 2,3 cyclophosphoryl-rhamnose attached to a ribose-branched sub-terminal rhamnose residue. The Ox247 genome contains a 24-kb locus containing genes for synthesis and protein attachment of this glycan. Mutations in genes within this locus altered or completely abrogated formation of this glycan, and their phenotypes suggested that this S-layer modification may affect sporulation, cell length, and biofilm formation of C. difficile. In summary, our findings indicate that the S-layer protein of SLCT-11 strains displays a complex glycan and suggest that this glycan is required for C. difficile sporulation and control of cell shape, a discovery with implications for the development of antimicrobials targeting the S-layer.

Therapy-related issues: endocrine system

2012 ◽  
Author(s):  
Philip Wiffen ◽  
Marc Mitchell ◽  
Melanie Snelling ◽  
Nicola Stoner
Keyword(s):  
Diabetes Mellitus ◽  
Endocrine System ◽  
Monitoring And Control ◽  
Cell Dysfunction ◽  
System P ◽  
The Uk ◽  
P Type ◽  
And Control

Diabetes mellitus 444Monitoring and control 449Thyroid disorders 450Diabetes mellitus (DM) affects approximately 4% of the UK population. In 2009, Diabetes UK reported that 2.6 million people in the UK have diabetes.Type 2 diabetes accounts for 90% of all diabetes and is a result of insulin resistance and pancreatic β-cell dysfunction. Type 1 diabetes results from an absolute insulin deficiency secondary to autoimmune dysfunction....

scholarly journals The Role of Gender in Potato Production and Marketing: Case of Sheka Zone, Southwest Ethiopia

2018 ◽  
Vol 4 (2) ◽  
pp. 16
Author(s):  
Feyere Bekele ◽  
Benyam Tadesse
Keyword(s):  
Potato Production ◽  
Relevant Information ◽  
Positive Influence ◽  
Southwest Ethiopia ◽  
Seed Collection ◽  
Land Preparation ◽  
Secondary Sources ◽  
Lack Of Control ◽  
And Control

The objective of the study was assessing gender roles in potato production and marketing in the study area. Relevant information was gathered from 193 potato producers by using primary and secondary sources of data. The data were analyzed by using descriptive statistics. The result indicated that in seed collection (46.3%), land preparation (60.3%), planting (96.8%), weeding (50.3%), cultivation (32.6%), Harvesting (38%), transporting (39.9%) and marketing (68.9%) of women respondents participate. Decisions about production and marketing of potato in the study area was mainly taken by men and in some cases, women participates. The main challenges that hinder gender participation in potato production and marketing was lack of access and control over assets(19.2%), lack of control of income(30.6%), Lack of land (6.7%) and lack of decision making on potato production(39.9%). Therefore, increasing opportunities for women, giving recognition, encouraging, documenting and disseminating their role can have a positive influence on productivity of potato.

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