Antioxidant and Chemoprevention Activity of Camelia Sinensis-Annona muricata Extract Combination against WiDr Cells Line

Antioxidant compounds have an essential role in inhibiting the process of cell proliferation and have a chemopreventive effect. This study aims to trace the presence of antioxidant compounds allegedly contained in tea leaves (Camelia Sinensis L.) and soursop leaves (Anonna muricata L.) and investigate their potency as chemopreventive agents. Research steps include (1) identify the active compounds using thin-layer chromatography (TLC); (2) find out the potential compounds against cancer cells by molecular docking using Autodock Vina; (3) conduct a potential antioxidant test using free radicals DPPH (1,1-diphenyl-2-pikrihidrazil); and (4) identify the cytotoxic effect on WiDr colon cancer cells test using MTT Assay method. The results showed that the ethanolic extract of Camellia sinensis-Annona muricata leaf combination was suspected of containing flavonoid compounds with Rf values of 0.66 and 0.68. Besides, the DPPH antioxidant test showed an IC50 value of 26.9 μg/mL. Cytotoxic potential against WiDr cells resulted in an IC50 value of 41 μg/mL. Furthermore, the molecular docking test of epigallocatechin gallate (EGCG) and Acetogenin compounds against bcl-xl target proteins showed the docking score of -8.1 kcal/mol and -6.7 kcal/mol, respectively. It concluded that the extract combination of Camelia Sinensis-Annona muricata leaf had strong potency as an antioxidant and chemopreventive agent against the WiDr colon cancer cells line.

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Cytotoxic Activity and Apoptosis Induction of Ethanolic Extract of Pericarps of Mangosteen (Garcinia mangostana Linn.) on WiDr Cells and Interaction Study of Alpha-mangosteen to IKK and VEGF Based on Molecular Docking

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev4iss1pp470-476 ◽

2013 ◽

Vol 4 (1) ◽

pp. 470

Author(s):

Annishfia Lailatur Rohmah ◽

Fikri Amalia ◽

Erlina Rivanti ◽

Dyaningtyas Dewi Pamungkas Putri ◽

Nunuk Aries Nurulita

Keyword(s):

Colon Cancer ◽

Molecular Docking ◽

Cancer Cells ◽

Cytotoxic Effect ◽

Ethanolic Extract ◽

Apoptosis Induction ◽

Colon Cancer Cells ◽

Garcinia Mangostana ◽

Double Staining Method ◽

Native Ligand

One of the compounds found efficacious as an anti-proliferative on colon cancer is alpha-mangosteen, a xanthon compound that is abundant in pericarp of mangosteen. This study focused to evaluate anticancer activity of the ethanolic extract of pericarp of mangosteen (Garcinia mangostana Linn.) on WiDr colon cancer cells and to observe the affinity of alpha-mangosteen in inhibiting IKK and VEGF. Cytotoxic effect was tested by MTT assay and apoptosis induction was evaluated by double staining method on WiDr colon cancer cells, while interaction between alpha-mangosteen to the receptors was measured by molecular docking. The ethanolic extract was proven to have cytotoxic activity against WiDr colon cancer cells with IC50 of 25 µg/mL. In addition, the observation of apoptosis induction by double-staining method showed that apoptosis associated the cytotoxic effect of ethanolic extract of pericarp of mangosteen (EPM) on WiDr colon cancer cells. Molecular docking showed that active compounds in pericarp of mangosteen could compete with the native ligand of VEGF because the docking score of alpha-mangosteen was almost equal with native ligand. From these results we could be concluded that the cytotoxic effect of EPM to WiDr cells was mediated by cell apoptosis. This extract was potential to be developed as chemopreventive agent.Keywords : Garcinia mangostana Linn., cytotoxic effect, apoptosis, WiDr cell, molecular docking

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Co-Chemotherapeutic Effect of n-Hexane Fraction of Binahong (Anredera cordifolia [Tenore] Steen.) on WiDr Colon Cancer Cell Line

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.5810 ◽

2021 ◽

Vol 9 (T4) ◽

pp. 77-82

Author(s):

Rifki Febriansah ◽

Harena Anggun Lakshita

Keyword(s):

Colon Cancer ◽

Molecular Docking ◽

Cancer Cells ◽

Hexane Fraction ◽

Flavonoid Compound ◽

Natural Material ◽

Phytochemical Screening ◽

Colon Cancer Cells ◽

Ic50 Value ◽

Cox 2

BACKGROUND: The incidence of colon cancer ranks the third most common cancer case in Indonesia, with 12.8 cases per 100.000 community. Problems that arise from cancer treatment with chemotherapy are severe side effects. Thus, we develop cancer therapy using a natural material, namely, binahong leaves (Anredera cordifolia). AIM: This study aims to investigate the co-chemotherapy activity of the n-hexane fraction of binahong (NFB) on the WiDr human colon cancer cells line. METHODS: The sample was prepared by maceration using ethanol 70% and fractionation using n-hexane. Phytochemical screening was conducted with the thin-layer chromatography method. The antioxidant test was carried out using 1,1 diphenyl-2-dipicrilhydrazil assay, while the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay was designed for cytotoxic tests on WiDr colon cancer cells to identify the single effect and its combination with 5-fluorouracil as chemotherapy agents. Further, in silico test, molecular docking was used to determine the 4’, 6, 7-trihydroxyaurone as an active compound on NFB against inhibitors of κB kinases (IKK) and COX-2 proteins. RESULTS: Phytochemical screening showed that NFB contained a flavonoid compound. NFB had a weak antioxidant activity with the inhibition concentration (IC50) value of 2851 μg/mL, while NFB had a relatively strong cytotoxic activity on WiDr colon cells with the IC50 value of 191 μg/mL, and had the synergist activity in combination with 5-fluorouracil as a chemotherapy agent. The molecular docking showed the best interaction of 4’, 6, 7-trihydroxyaurone against IKK, and COX-2 proteins with docking scores of −9.0 kcal/mol and −8.1 kcal/mol, respectively. CONCLUSION: NFB had the potential to inhibit cell growth and had a synergistic effect in combination with 5-fluorouracil on WiDr colon cancer cells line.

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Human Colon Cancer Cells Undergo Apoptosis by Theaflavin Digallate, Epigallocatechin Gallate, and Oolong Tea Polyphenol Extract

Journal of Herbs Spices & Medicinal Plants ◽

10.1300/j044v10n04_04 ◽

2004 ◽

Vol 10 (4) ◽

pp. 29-38 ◽

Cited By ~ 3

Author(s):

Hiroshige Hibasami ◽

Zong-Xuan Jin ◽

Kazumi Yoshioka ◽

Kohko Ina ◽

Kazuko Ohnishi

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Epigallocatechin Gallate ◽

Human Colon ◽

Colon Cancer Cells ◽

Tea Polyphenol ◽

Human Colon Cancer ◽

Oolong Tea ◽

Human Colon Cancer Cells

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Annona muricata leaves induce G1 cell cycle arrest and apoptosis through mitochondria-mediated pathway in human HCT-116 and HT-29 colon cancer cells

Journal of Ethnopharmacology ◽

10.1016/j.jep.2014.08.011 ◽

2014 ◽

Vol 156 ◽

pp. 277-289 ◽

Cited By ~ 55

Author(s):

Soheil Zorofchian Moghadamtousi ◽

Hamed Karimian ◽

Elham Rouhollahi ◽

Mohammadjavad Paydar ◽

Mehran Fadaeinasab ◽

...

Keyword(s):

Cell Cycle ◽

Colon Cancer ◽

Cell Cycle Arrest ◽

Cancer Cells ◽

Annona Muricata ◽

Colon Cancer Cells ◽

Cycle Arrest ◽

Hct 116 ◽

G1 Cell Cycle Arrest ◽

Ht 29

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Tetrandrine inhibits colon carcinoma HT-29 cells growth via the Bcl-2/Caspase 3/PARP pathway and G1/S phase

Bioscience Reports ◽

10.1042/bsr20182109 ◽

2019 ◽

Vol 39 (5) ◽

Cited By ~ 10

Author(s):

JiaNan Li ◽

QiuHong Wang ◽

ZhiBin Wang ◽

Na Cui ◽

BingYou Yang ◽

...

Keyword(s):

Colon Cancer ◽

Molecular Docking ◽

Cancer Cells ◽

Caspase 3 ◽

Caspase 8 ◽

Therapeutic Effects ◽

Dependent Manner ◽

Colon Cancer Cells ◽

Ht 29 ◽

Active Caspase

Abstract Tetrandrine (Tet) bisbenzylisoquinoline alkaloids isolated from Stephania tetrandra and other related species of Menispermaceae. It has been demonstrated to have positive therapeutic effects on cardiovascular disease, hypertension, silicosis, autoimmune diseases. In recent years, some reports have shown that Tet has anticancer activity in human cancers. To explore the pharmacological activity and mechanism of Tet on colon cancer and its unique advantages as a natural product. In the present study, analyses of the cell cycle, apoptosis, targets prediction, molecular docking, and alterations in protein levels were performed to elucidate how Tet functions in colon cancer. We found that Tet robustly induced arrest at the G1 phase in colon cancer cell line HT-29. It induced HT-29 cell apoptosis in a dose-dependent manner. Similarly, analysis of protein expression levels in HT-29 cells showed down-regulation of Bcl-2, pro-caspase 3, pro-caspase 8, PARP, cyclin D1 (CCND1), cyclin-dependent kinase 4 (CDK 4), and up-regulation of Bax, active caspase 3, and active caspase 8. These results indicate that Tet induces apoptosis of colon cancer cells through the mitochondrial pathway and caspase family pathway. Molecular docking showed interaction effects and binding energy. Comparing with the CDK4 inhibitors ribociclib and palbociclib, the docking energy is similar to the docked amino acid residues. Therefore, we conclude that Tet and the CCND1/CDK4 compound could form hydrogen bonds and a stable compound structure, which can inhibit colon cancer cells proliferation by regulating CCND1/CDK4 compound and its downstream proteins phosphorylated Rb (p-Rb). In summary, Tet may be a potential drug for colon cancer therapy.

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Anticarcinogenic Effects of the Ethanolic Extract ofSalix aegyptiacain Colon Cancer Cells: Involvement of Akt/PKB and MAPK Pathways

Nutrition and Cancer ◽

10.1080/01635581.2013.850966 ◽

2013 ◽

Vol 65 (7) ◽

pp. 1045-1058 ◽

Cited By ~ 16

Author(s):

Shabnam Enayat ◽

Müşerref Şeyma Ceyhan ◽

Arif Ahmet Başaran ◽

Mayda Gürsel ◽

Sreeparna Banerjee

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Ethanolic Extract ◽

Colon Cancer Cells ◽

Mapk Pathways

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Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev7iss2pp60-67 ◽

2017 ◽

Vol 7 (2) ◽

pp. 60

Author(s):

Erlina Rivanti ◽

Bani Adlina Shabrina ◽

Ika Nurzijah ◽

Cyndwika Ayu ◽

Adam Hermawan

Keyword(s):

Cancer Cells ◽

Hela Cells ◽

Breast Cancer Cells ◽

Ethanolic Extract ◽

Vero Cells ◽

Cytotoxic Activities ◽

Colon Cancer Cells ◽

Caesalpinia Sappan ◽

Ic50 Value ◽

Cervical Cancer Cells

The present study investigate the selectivity of heartwood of secang ethanolic extract (SEE) on T47D breast cancer cells, WiDr colon cancer cells, and HeLa cervical cancer cells, compared to Vero normal epithelial cells. The cytotoxic effect was evaluated by using MTT assay with 24-hour treatment to get IC50values. Selectivity was evaluated by using selectivity index (SI). SEE had a potent cytotoxic activity on T47D and WiDr cancer cells (IC50 <100 µg/ml). IC50 value of HeLa cancer cells was observed on moderate cytotoxic (100 <IC50 <1000 µg/ml). SEE demonstrated more selective to T47D and WiDr than Vero cells (SI > 3), while in HeLa cells is not selective (SI < 3). This result indicating its potential of Caesalpinia sappan as a chemopreventive agent in cancer therapy.Keywords: Cancer, selectivity, Secang, T47D, WiDr, HeLa, Vero

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Ethanolic Extract of Citrus reticulata Peel Inhibits the Migration of WiDr Colon Cancer Cells

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev11iss2pp60-66 ◽

2020 ◽

Vol 11 (2) ◽

pp. 60

Author(s):

Yoni Astuti ◽

Aulia Primasari

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Migration And Invasion ◽

Citrus Reticulata ◽

Colon Cancer Cells ◽

Migration Assay ◽

Widr Cell ◽

Cancer Line

Colorectal cancer is third rank on the cancer cases in Indonesia. To cure the cancer needs big cost and lot of effort. On the other side, the side effect of medicine or chemotherapy on patient need to reduce. Cancer cell spread to other tissue based on its migration and invasion ability. Citrus reticulata peel contains flavonoid such as Tangeretin and Nobiletin, both of this compounds have anticancer activity. The aims of this study is to reveals the potency of ethanol extract of Citrus reticulata peel on the inhibition of migration on WiDr colon cancer cells. The toxicity of ethanol extract of Citurs reticulata peel on WiDr colon cancer line was measured using 3-(4,5-dimethyltiazol-2-il)-2,5-diphenyltrazolium bromide (MTT) assay and investigate the cell migration was using scratch wound healing assay. The ethanol extract of Citrus reticulata peel showed the value of inhibitory concentration 50 (IC50) was 184.5 μg/mL, this result categorize as moderate cytotoxic. Meanwhile the migration assay showed that the deceleration of migration occurred on 0.5 IC50, 0.33 IC50 and 0.25 IC50 during 24 h and 36 h incubation, event thought there were not significant different (p>0.05). The ethanol extract of Citrus reticulata peel has a potential migration inhibition on WiDr cell line.Keywords: Citrus reticulata, WiDr cell line, migration

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