Role of BMAL1 and CLOCK in regulating the secretion of melatonin in chick retina under monochromatic green light

UnaG is a new class of fluorescence protein for which an endogenous ligand namely bilirubin (BLR) is playing the role of the chromophore. Upon photoexcitation, holoUnaG emits the green light....

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Role of Blue-green-light Filter in Detecting a Spontaneous Cerebrospinal Fluid Rhinorrhea in an Unusual Site

Clinical Rhinology An International Journal ◽

10.5005/jp-journals-10013-1344 ◽

2018 ◽

Vol 11 (2-3) ◽

pp. 52-54

Author(s):

Jyotirmay S Hegde ◽

Sunil K Saxena ◽

Natarajan Ramalingam ◽

Nithin P Nair

Keyword(s):

Cerebrospinal Fluid ◽

Green Light ◽

Light Filter ◽

Unusual Site ◽

Cerebrospinal Fluid Rhinorrhea

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Kinetics of Plasmon-Driven Hydrosilylation of Silicon Surfaces: Photogenerated Charges Drive Silicon- Carbon Bond Formation

10.26434/chemrxiv.14700762 ◽

2021 ◽

Author(s):

Chengcheng Rao ◽

Brian Olsen ◽

Erik Luber ◽

Jillian Buriak

Keyword(s):

Green Light ◽

Kinetic Studies ◽

Silicon Surfaces ◽

Moderate Intensity ◽

Carbon Bond ◽

Silicon Carbon ◽

Set Up ◽

Kinetics Of ◽

Insight Into

Optically transparent PDMS stamps coated with a layer of gold nanoparticles were employed as plasmonic stamps to drive surface chemistry on silicon surfaces. Illumination of a sandwich of plasmonic stamps, an alkene ink, and hydride-terminated silicon with green light of moderate intensity drives hydrosilylation on the surface. The key to the mechanism of the hydrosilylation is the presence of holes at the Si-H-terminated interface, which is followed by attack by a proximal alkene and formation of the silicon-carbon bond. In this work, detailed kinetic studies of the hydrosilylation on silicon with different doping levels, n++, p++, n, p, and intrinsic were carried out to provide further insight into the role of the metal-insulator-semiconductor (MIS) junction that is set up during the stamping.

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NF-κB signaling regulates the formation of proliferating Müller glia-derived progenitor cells in the avian retina

10.1101/724260 ◽

2019 ◽

Cited By ~ 2

Author(s):

Isabella Palazzo ◽

Kyle Deistler ◽

Thanh V. Hoang ◽

Seth Blackshaw ◽

Andy J. Fischer

Keyword(s):

Progenitor Cells ◽

Nuclear Factor Kappa B ◽

Neuronal Damage ◽

Muller Glia ◽

Müller Glia ◽

Reactive Microglia ◽

Chick Retina ◽

Cell Signaling Pathways

AbstractNeuronal regeneration in the retina is a robust, effective process in some cold-blooded vertebrates, but this process is ineffective in warm-blooded vertebrates. Understanding the mechanisms and cell-signaling pathways that restrict the reprogramming of Müller glia into proliferating neurogenic progenitors is key to harnessing the regenerative potential of the retina. Inflammation and reactive microglia are known to influence the formation of Müller glia-derived progenitor cells (MGPCs), but the mechanisms underlying this response are unknown. Using the chick retina in vivo as a model system, we investigate the role of the Nuclear Factor kappa B (NF-κB) signaling, a critical regulator of inflammation. We find that components of the NF-κB pathway are expressed by Müller glia and are dynamically regulated after neuronal damage or treatment with growth factors. Inhibition of NF-κB enhances, whereas activation suppresses the formation of proliferating MGPCs. Additionally, activation of NF-κB promotes glial differentiation from MGPCs in damaged retinas. With microglia ablated, the effects of NF-κB-agonists/antagonists on MGPC formation are reversed, suggesting that the context and timing of signals provided by reactive microglia influence how NF-κB-signaling impacts the reprogramming of Müller glia. We propose that NF-κB-signaling is an important signaling “hub” that suppresses the reprogramming of Müller glia into proliferating MGPCs and this “hub” coordinates signals provided by reactive microglia.

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Kinetics of Plasmon-Driven Hydrosilylation of Silicon Surfaces: Photogenerated Charges Drive Silicon- Carbon Bond Formation

10.26434/chemrxiv.14700762.v1 ◽

2021 ◽

Author(s):

Chengcheng Rao ◽

Brian Olsen ◽

Erik Luber ◽

Jillian Buriak

Keyword(s):

Green Light ◽

Kinetic Studies ◽

Silicon Surfaces ◽

Moderate Intensity ◽

Carbon Bond ◽

Silicon Carbon ◽

Set Up ◽

Kinetics Of ◽

Insight Into

Optically transparent PDMS stamps coated with a layer of gold nanoparticles were employed as plasmonic stamps to drive surface chemistry on silicon surfaces. Illumination of a sandwich of plasmonic stamps, an alkene ink, and hydride-terminated silicon with green light of moderate intensity drives hydrosilylation on the surface. The key to the mechanism of the hydrosilylation is the presence of holes at the Si-H-terminated interface, which is followed by attack by a proximal alkene and formation of the silicon-carbon bond. In this work, detailed kinetic studies of the hydrosilylation on silicon with different doping levels, n++, p++, n, p, and intrinsic were carried out to provide further insight into the role of the metal-insulator-semiconductor (MIS) junction that is set up during the stamping.

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Antisera to basal lamina and glial endfeet disturb the normal extension of axons on retina and pigment epithelium basal laminae

Development ◽

10.1242/dev.107.2.281 ◽

1989 ◽

Vol 107 (2) ◽

pp. 281-297

Author(s):

W. Halfter

Keyword(s):

Basal Lamina ◽

Pigment Epithelium ◽

Growth Cones ◽

Normal Extension ◽

Embryonic Chick ◽

Adhesive Matrix ◽

Chick Retina ◽

Directed Growth ◽

The Individual

In order to determine the role of the extracellular matrix in regulating the directed growth of embryonic neurites, antisera to retina (a-RBL I and II), to pigment epithelium (a-PBL) and to glomerular (a-GBL) basal lamina were probed for an effect on the ordered extension of neurites. In the assays, retina explants from chick and quail were cultured on basal lamina from embryonic chick retina and pigment epithelium either in the presence of anti-basal lamina antisera or in the presence of the corresponding preimmune sera. In the presence of all anti-basal lamina antisera, normal extension of axons was greatly inhibited both on retina and on pigment epithelium basal lamina. The antisera affected the growth pattern and the morphology of the individual axons in two ways: in the presence of a-RBL I the short axons were less directed, developed more and longer side branches, and the lamellipodia of the growth cones were reduced in size compared to axons from control cultures. In the presence of a-RBL II and a-GBL, axons grew slowly out from the explants as very thick bundles, strikingly different from axons in control cultures. The antiserum to pigment epithelium basal lamina induced both strong fasciculation and disorganization of the linear fiber extension, being intermediate between the two types of effects observed after antiserum addition. The results suggest that adhesive matrix molecules in basal laminae have important functions in elongation, fasciculation and in the morphology of growing axons.

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Green Light to Plant Responses to Pathogens: The Role of Chloroplast Light-Dependent Signaling in Biotic Stress

Photochemistry and Photobiology ◽

10.1111/php.12466 ◽

2015 ◽

Vol 91 (5) ◽

pp. 1004-1011 ◽

Cited By ~ 16

Author(s):

María Laura Delprato ◽

Adriana R. Krapp ◽

Néstor Carrillo

Keyword(s):

Biotic Stress ◽

Green Light ◽

Plant Responses

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Role of the cell recognition molecule, cognin, in GABAergic differentiation in chick retina

Brain Research ◽

10.1016/0006-8993(92)91286-n ◽

1992 ◽

Vol 589 (2) ◽

pp. 268-274 ◽

Cited By ~ 9

Author(s):

Bukhtiar H. Shah ◽

A.S.M. Krishna Rao ◽

R.E. Hausman

Keyword(s):

Cell Recognition ◽

Chick Retina ◽

Recognition Molecule

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BMAL1 but not CLOCK is associated with monochromatic green light-induced circadian rhythm of melatonin in chick pinealocytes

Endocrine Connections ◽

10.1530/ec-18-0377 ◽

2019 ◽

Vol 8 (1) ◽

pp. 57-68 ◽

Cited By ~ 2

Author(s):

Shuhui Ma ◽

Zixu Wang ◽

Jing Cao ◽

Yulan Dong ◽

Yaoxing Chen

Keyword(s):

Circadian Rhythm ◽

Circadian Rhythms ◽

Pineal Gland ◽

Clock Genes ◽

Critical Role ◽

Green Light ◽

Circadian Oscillator ◽

Melatonin Secretion ◽

Expression Levels

The avian pineal gland, an independent circadian oscillator, receives external photic cues and translates them for the rhythmical synthesis of melatonin. Our previous study found that monochromatic green light could increase the secretion of melatonin and expression of CLOCK and BMAL1 in chick pinealocytes. This study further investigated the role of BMAL1 and CLOCK in monochromatic green light-induced melatonin secretion in chick pinealocytes using siRNAs interference and overexpression techniques. The results showed that si-BMAL1 destroyed the circadian rhythms of AANAT and melatonin, along with the disruption of the expression of all the seven clock genes, except CRY1. Furthermore, overexpression of BMAL1 also disturbed the circadian rhythms of AANAT and melatonin, in addition to causing arrhythmic expression of BMAL1 and CRY1/2, but had no effect on the circadian rhythms of CLOCK, BMAL2 and PER2/3. The knockdown or overexpression of CLOCK had no impact on the circadian rhythms of AANAT, melatonin, BMAL1 and PER2, but it significantly deregulated the circadian rhythms of CLOCK, BMAL2, CRY1/2 and PER3. These results suggested that BMAL1 rather than CLOCK plays a critical role in the regulation of monochromatic green light-induced melatonin rhythm synthesis in chicken pinealocytes. Moreover, both knockdown and overexpression of BMAL1 could change the expression levels of CRY2, it indicated CRY2 may be involved in the BMAL1 pathway by modulating the circadian rhythms of AANAT and melatonin.

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