Short-Term Effects of Albendazole on the Oxidative Stress Markers and Hematological Parameters in Tissues of African CatfishClarias gariepinus

Oxidative stress and inflammation are implicated in obesity. Therefore, we investigated whether moderate and short-term calorie restriction (CR) reflects a real-life situation, mediates weight loss, and improves oxidative stress markers. We analyzed oxidative stress markers in patients with obesity undergoing moderate CR. Serum oxidative stress markers (myeloperoxidase (MPO), superoxide dismutase (SOD), catalase, total antioxidant status (TAS), and reactive oxygen species (ROS) (generation by endothelial cells in vitro)) were measured in 53 subjects (mean BMI 37.8 ± 5.9 kg/m2) who underwent 8 weeks of CR, which included a reduction of 300–500 kcal/day. MPO was the most CR-sensitive parameter. The mean level of serum MPO in patients with obesity was 20% higher than that in post CR intervention (p < 0.001). SOD increased by 12% after CR (p < 0.05), which was largely due to the improvement in glucose tolerance and the reduction in insulin resistance after CR. Other tested parameters were not modified during the treatment. CR resulted in an expected decrease in body weight (by 5.9 ± 4.6 kg, p < 0.0001) and other anthropometric parameters. Additionally, it was accompanied by a significant change in hsCRP, hsTNF alpha, hsIL-6, leptin (all p < 0.0001), and HOMA-IR (p < 0.05). Cardiovascular and metabolic parameters were also partially improved. Short-term, moderate CR partially improves antioxidant capacity but is enough to substantially change anthropometric parameters in obese patients. Our observations indicate that mimicking real-life situations and low-cost dietary intervention can be successfully implemented in obesity treatment with a simultaneous moderate effect on antioxidant status.

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Graphene Oxide Normal (GO + Mn2+) and Ultrapure: Short-Term Impact on Selected Antioxidant Stress Markers and Cytokines in NHDF and A549 Cell Lines

Antioxidants ◽

10.3390/antiox10050765 ◽

2021 ◽

Vol 10 (5) ◽

pp. 765

Author(s):

Dominika Stygar ◽

Aleksandra Pogorzelska ◽

Elżbieta Chełmecka ◽

Bronisława Skrzep-Poloczek ◽

Barbara Bażanów ◽

...

Keyword(s):

Oxidative Stress ◽

Graphene Oxide ◽

Cell Lines ◽

A549 Cell ◽

A549 Cells ◽

Dermal Fibroblasts ◽

Oxidative Stress Markers ◽

Short Term ◽

Necrosis Factor Alpha ◽

Stress Markers

Since biological applications and toxicity of graphene-based materials are structure dependent, studying their interactions with the biological systems is very timely and important. We studied short-term (1, 24, and 48 h) effects of ultrapure (GO) and Mn2+-contaminated (GOS) graphene oxide on normal human dermal fibroblasts (NHDF) and adenocarcinomic human alveolar basal epithelial cells (A549) using selected oxidative stress markers and cytokines: glutathione reductase (GR) and catalase (CAT) activity, total antioxidative capacity (TAC), and malondialdehyde (MDA) concentration, levels of vascular endothelial growing factor (VEGF), tumor necrosis factor-alpha (TNF-a), platelet-derived growth factor-BB (PDGF-BB), and eotaxin. GOS induced higher levels of oxidative stress, measured with CAT activity, TAC, and MDA concentration than GO in both cell lines when compared to control cells. GR activity decreased in time in NHDF cells but increased in A549 cells. The levels of cytokines were related to the exposure time and graphene oxide type in both analyzed cell lines and their levels comparably increased over time. We observed higher TNF-a levels in NHDF and higher levels of VEGF and eotaxin in the A549 cell line. Both types of cells showed similar susceptibility to GO and GOS. We concluded that the short-time exposure to GOS induced the stronger response of oxidative stress markers without collapsing the antioxidative systems of analysed cells. Increased levels of inflammatory cytokines after GO and GOS exposure were similar both in NHDF and A549 cells.

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Short-term glucose variability in healthy volunteers is not associated with raised oxidative stress markers

Diabetes Obesity and Metabolism ◽

10.1111/j.1463-1326.2012.01625.x ◽

2012 ◽

Vol 14 (11) ◽

pp. 1047-1049 ◽

Cited By ~ 7

Author(s):

A. Wakil ◽

K. A. Smith ◽

S. L. Atkin ◽

E. S. Kilpatrick

Keyword(s):

Oxidative Stress ◽

Healthy Volunteers ◽

Glucose Variability ◽

Oxidative Stress Markers ◽

Short Term ◽

Stress Markers

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Blood Oxidative Stress Markers During a Short Term Trek on Mount Rainier

Medicine & Science in Sports & Exercise ◽

10.1249/01.mss.0000400780.20978.e5 ◽

2011 ◽

Vol 43 (Suppl 1) ◽

pp. 284

Author(s):

Lindsey E. Miller ◽

Graham McGinnis ◽

Brian Kliszeczewicz ◽

Dustin Slivka ◽

Walter Hailes ◽

...

Keyword(s):

Oxidative Stress ◽

Oxidative Stress Markers ◽

Short Term ◽

Stress Markers ◽

Mount Rainier

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Effects of Spiruline (Spirulina platensis) Supplementation on Oxidative Stress Markers, Biochemical Characteristics, and Hematological Parameters in Rabbit (Oryctolagus cuniculus) Does

Asian Journal of Agriculture and Rural Development ◽

10.18488/journal.ajard.2021.112.163.170 ◽

2021 ◽

Vol 11 (2) ◽

pp. 163-170

Author(s):

Chongsi Margaret Mary Momo ◽

Mahamat Tahir Markhous Adam ◽

Tchoffo Herve ◽

Vemo Bertin Narcisse ◽

Ngoula Ferdinand ◽

...

Keyword(s):

Oxidative Stress ◽

Hematological Parameters ◽

Control Group ◽

Oxidative Stress Markers ◽

Feed Supplementation ◽

Biochemical Characteristics ◽

Oxidative Stress Parameters ◽

Stress Markers ◽

Sexually Mature ◽

Stress Parameters

The present study was carried out in order to evaluate the effects of feed supplementation with spiruline powder on the oxidative stress markers, biochemical characteristics, and hematological parameters in rabbit doe. Twenty-one nulliparous and sexually mature does (7–8 months old) were distributed into three groups of seven does each, comparable in terms of body weight. After a week of feeding with experimental feed, does of each group were mated. During the trial, does of group 1 (control group) were fed ad libitum with a feed free from spiruline (control feed), while those of groups 2 and 3 received the control feed supplemented with spiruline powder at 0.6 and 1.2%, respectively. Immediately after parturition, blood was collected for analyses of hematological, biochemical, and oxidative stress parameters. Studied parameters included serum concentrations of total proteins, cholesterol, aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), creatinine, and urea; malondialdehyde, reduced glutathione, catalase, and superoxide dismutase; and hematological parameters. The total protein concentration, number of white and red blood cells, platelets, hemoglobin, hematocrit, and mean corpuscular volume were significantly (P<0.05) higher in does treated compared to control. Oxidative stress parameters were comparable (P>0.05) among treatments. Hence, these results show that feed supplementation with spirulina powder can improve rabbit doe health, notably at 1.2%, since optimal results were obtained at this percentage.

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