scholarly journals A framework for standardized qPCR-targets and protocols for quantifying antibiotic resistance in surface water, recycled water and wastewater

2022 ◽  
pp. 1-25
Author(s):  
Ishi Keenum ◽  
Krista Liguori ◽  
Jeanette Calarco ◽  
Benjamin C. Davis ◽  
Erin Milligan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Surface Water ◽  
Recycled Water ◽  
Water And Wastewater

Antibiotic resistance genes in surface water and groundwater from mining affected environments

2021 ◽  
Vol 772 ◽  
pp. 145516
Author(s):  
Hai-Yan Zou ◽  
Liang-Ying He ◽  
Fang-Zhou Gao ◽  
Min Zhang ◽  
Shuai Chen ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Surface Water ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Surface Water And Groundwater

Determination of antibiotic resistance genes in a WWTP-impacted river in surface water, sediment, and biofilm: Influence of seasonality and water quality

2021 ◽  
pp. 144526
Author(s):  
Gabriela Reichert ◽  
Stephan Hilgert ◽  
Johannes Alexander ◽  
Júlio César Rodrigues de Azevedo ◽  
Tobias Morck ◽  
...  
Keyword(s):  
Water Quality ◽  
Antibiotic Resistance ◽  
Surface Water ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes

scholarly journals Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia

1999 ◽  
Vol 123 (2) ◽  
pp. 225-232 ◽  
Author(s):  
S. RADU ◽  
Y. K. HO ◽  
S. LIHAN ◽  
YUHERMAN ◽  
G. RUSUL ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Surface Water ◽  
Vibrio Cholerae ◽  
Genetic Relatedness ◽  
Profile Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Plasmid Profile ◽  
Significant Public Health ◽  
Resistance Patterns ◽  
Wide Variability

A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water) and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and 1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based assays, resistance to antibiotics, plasmid profiles and random amplified polymorphic DNA (RAPD) analysis. All were resistant to 9 or more of the 17 antibiotics tested. Identical antibiotic resistance patterns of the isolates may indicate that they share a common mode of developing antibiotic resistance. Furthermore, the multiple antibiotic resistance indexing showed that all strains tested originated from high risk contamination. Plasmid profile analysis by agarose gel electrophoresis showed the presence of small plasmids in 12 (7 non-O1 and 5 O1 serotypes) with sizes ranging 1·3–4·6 MDa. The CT gene was detected in all clinical isolates but was present in only 14 (6 O1 serotype and 8 non-O1 serotype) isolates from environmental waters. The genetic relatedness of the clinical and environmental Vibrio cholerae O1 and non-O1 strains was investigated by RAPD fingerprinting with four primers. The four primers generated polymorphisms in all 31 strains of Vibrio cholerae tested, producing bands ranging from <250 to 4500 bp. The RAPD profiles revealed a wide variability and no correlation with the source of isolation. This study provides evidence that Vibrio cholerae O1 and non-O1 have significant public health implications.

scholarly journals Comparison of Culture- and Quantitative PCR-Based Indicators of Antibiotic Resistance in Wastewater, Recycled Water, and Tap Water

2019 ◽  
Vol 16 (21) ◽  
pp. 4217 ◽  
Author(s):  
Jaqueline Rocha ◽  
Telma Fernandes ◽  
Maria V. Riquelme ◽  
Ni Zhu ◽  
Amy Pruden ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Tap Water ◽  
Quantitative Polymerase Chain Reaction ◽  
Antibiotic Resistance Genes ◽  
Fecal Coliforms ◽  
Future Application ◽  
Strongly Correlated ◽  
Recycled Water ◽  
The Us ◽  
Genes Encoding

Standardized methods are needed to support monitoring of antibiotic resistance in environmental samples. Culture-based methods target species of human-health relevance, while the direct quantification of antibiotic resistance genes (ARGs) measures the antibiotic resistance potential in the microbial community. This study compared measurements of tetracycline-, sulphonamide-, and cefotaxime-resistant presumptive total and fecal coliforms and presumptive enterococci versus a suite of ARGs quantified by quantitative polymerase chain reaction (qPCR) across waste-, recycled-, tap-, and freshwater. Cross-laboratory comparison of results involved measurements on samples collected and analysed in the US and Portugal. The same DNA extracts analysed in the US and Portugal produced comparable qPCR results (variation <28%), except for blaOXA-1 gene (0%–57%). Presumptive total and fecal coliforms and cefotaxime-resistant total coliforms strongly correlated with blaCTX-M and intI1 (0.725 ≤ R2 ≤ 0.762; p < 0.0001). Further, presumptive total and fecal coliforms correlated with the Escherichia coli-specific biomarkers, gadAB, and uidA, suggesting that both methods captured fecal-sourced bacteria. The genes encoding resistance to sulphonamides (sul1 and sul2) were the most abundant, followed by genes encoding resistance to tetracyclines (tet(A) and tet(O)) and β-lactams (blaOXA-1 and, blaCTX-M), which was in agreement with the culture-based enumerations. The findings can help inform future application of methods being considered for international antibiotic resistance surveillance in the environment.

scholarly journals Antibiotic Residue in the Aquatic Environment: Status in Africa

2018 ◽  
Vol 16 (1) ◽  
pp. 890-903 ◽  
Author(s):  
A.C. Faleye ◽  
A.A. Adegoke ◽  
K. Ramluckan ◽  
Faizal Bux ◽  
T. A. Stenström
Keyword(s):  
Antibiotic Resistance ◽  
Surface Water ◽  
Aquatic Environment ◽  
Human Population ◽  
Potable Water ◽  
Wastewater Treatment Plants ◽  
Fecal Matter ◽  
Sanitation Systems ◽  
Poor Nutrition ◽  
Antibiotic Residue

AbstractInformation on the presence of antibiotics is sparse for all types of water in Africa, including groundwater, surface water, effluent of wastewater treatment plants (WWTPs) and municipal potable water. With the relatively high sales of different antibiotics to treat infectious diseases in the human population of Africa, the residual of the antibiotics is bound to be released through excretion via urine or fecal matter in parallel to the high sales. This article reviews the published analysis on the occurrence of antibiotics in the environment particularly in the aquatic environment in some countries in Africa. In general, sulfamethoxazole was the most commonly detected in Africa surface water (with eight reports from four countries) at a concentration range of 0.00027 – 39 μgL-1. Wastewater analysis is believed to give an early warning for preventing epidemics. Thus, we discuss the associated level of antibiotic resistance to some prevalent diseases in Africa whose aetiological agents can develop antibiotic resistance due to exposure to antibiotic residue in water. This is important because of rising population of immuno-deficient African residents ravaged by HIV/AIDS, poor nutrition and less efficient sanitation systems.

Sign in / Sign up

Export Citation Format

Share Document