Identification of culturable petroleum-degrading bacteria and fungi from petroleum-contaminated sites in Brunei Darussalam

2020 ◽  
Vol 55 (13) ◽  
pp. 1542-1547
Author(s):  
Hussein Taha ◽  
Pooja Shivanand ◽  
De Hwa Khoo ◽  
Yumni Haziqah Mohammad ◽  
Nur Bazilah Afifah Matussin ◽  
...  
Keyword(s):  
Contaminated Sites ◽  
Brunei Darussalam ◽  
Degrading Bacteria ◽  
Bacteria And Fungi

scholarly journals Soil microbiota and microarthropod communities in oil contaminated sites in the European Subarctic

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
E. N. Melekhina ◽  
E. S. Belykh ◽  
M. Yu. Markarova ◽  
A. A. Taskaeva ◽  
E. E. Rasova ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Taxonomic Diversity ◽  
Northern Taiga ◽  
Contaminated Sites ◽  
Oil Contamination ◽  
Polluted Soils ◽  
Degrading Bacteria ◽  
Upper Level ◽  
Bacteria And Fungi ◽  
Soil Level

AbstractThe present comprehensive study aimed to estimate the aftermath of oil contamination and the efficacy of removing the upper level of polluted soil under the conditions of the extreme northern taiga of northeastern European Russia. Soil samples from three sites were studied. Two sites were contaminated with the contents of a nearby sludge collector five years prior to sampling. The highly contaminated upper soil level was removed from one of them. The other was left for self-restoration. A chemical analysis of the soils was conducted, and changes in the composition of the soil zoocoenosis and bacterial and fungal microbiota were investigated. At both contaminated sites, a decrease in the abundance and taxonomic diversity of indicator groups of soil fauna, oribatid mites and collembolans compared to the background site were found. The pioneer eurytopic species Oppiella nova, Proisotoma minima and Xenyllodes armatus formed the basis of the microarthropod populations in the contaminated soil. A complete change in the composition of dominant taxonomic units was observed in the microbiota, both the bacterial and fungal communities. There was an increase in the proportion of representatives of Proteobacteria and Actinobacteria in polluted soils compared to the background community. Hydrocarbon-degrading bacteria—Alcanivorax, Rhodanobacter ginsengisoli, Acidobacterium capsulatum, and Acidocella—and fungi—Amorphotheca resinae abundances greatly increased in oil-contaminated soil. Moreover, among both bacteria and fungi, a sharp increase in the abundance of uncultivated organisms that deserve additional attention as potential oil degraders or organisms with a high resistance to oil contamination were observed. The removal of the upper soil level was partly effective in terms of decreasing the oil product concentration (from approximately 21 to 2.6 g/kg of soil) and preventing a decrease in taxonomic richness but did not prevent alterations in the composition of the microbiota or zoocoenosis.

Enrichment and Characterization of Hydrocarbon Degrading Bacteria from Various Oil-Contaminated Sites in Pakistan

2021 ◽  
pp. 1-11
Author(s):  
Amna Aqeel ◽  
Zahid Hussain ◽  
Qurat-Ul-Ain Aqeel ◽  
Javaria Zafar ◽  
Naureen Ehsan ◽  
...  
Keyword(s):  
Contaminated Sites ◽  
Degrading Bacteria

scholarly journals Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Christabel Ndahebwa Muhonja ◽  
Gabriel Magoma ◽  
Mabel Imbuga ◽  
Huxley Mae Makonde
Keyword(s):  
Bacillus Cereus ◽  
Aspergillus Oryzae ◽  
Bacterial Degradation ◽  
Low Density ◽  
Optimum Growth ◽  
Alkane Hydroxylase ◽  
Fungal Isolates ◽  
Degrading Bacteria ◽  
Bacteria And Fungi

This study aimed at molecular and biochemical characterization of low-density polyethene (LDPE) degrading fungi and bacteria from Dandora dumpsite, Nairobi. Twenty bacterial and 10 fungal isolates were identified using 16S rDNA and 18S rDNA sequences for bacteria and fungi, respectively. The highest fungal degradation was attributed to Aspergillus oryzae strain A5,1 while the highest bacterial degradation was attributed to Bacillus cereus strain A5,a and Brevibacillus borstelensis strain B2,2, respectively. Isolates were screened for their ability to produce extracellular laccase and esterase; Aspergillus fumigatus strain B2,2 exhibited the highest presence of laccase (15.67 mm) while Aspergillus oryzae strain A5,1 exhibited the highest presence of esterase (14.33 mm). Alkane hydroxylase-encoding genes were screened for using primer AlkB 1 which amplified the fragment of size 870 bp. Four bacterial samples were positive for the gene. Optimum growth temperature of the fungal isolates was 30°C. The possession of laccase, esterase, and alkane hydroxylase activities is suggested as key molecular basis for LDPE degrading capacity. Knowledge of optimum growth conditions will serve to better utilize microbes in the bioremediation of LDPE. The application of Aspergillus oryzae strain A5,1 and Bacillus cereus strain A5,a in polyethene degradation is a promising option in this kind of bioremediation as they exhibited significantly high levels of biodegradation. Further investigation of more alkane degrading genes in biodegrading microbes will inform the choice of the right microbial consortia for bioaugmentation strategies.

scholarly journals Isolation and Identification of Phenol-Degrading Bacteria from Oil-Contaminated Sites

2021 ◽  
Vol 38 (1) ◽  
pp. 160-165
Author(s):  
Z.M. Usman ◽  
M.A. Said ◽  
F.A. Shehu ◽  
K. Abdussalam ◽  
T.M. Abdulrazak ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
System Analysis ◽  
Bacterial Species ◽  
Phenol Degradation ◽  
Contaminated Sites ◽  
Isolation And Identification ◽  
Selective Enrichment ◽  
Enrichment Technique ◽  
Degrading Bacteria ◽  
Cupriavidus Pauculus

This work is aimed at isolating and identifying phenol-degrading bacteria from oil-contaminated sites. Five soil samples from three auto-mechanic workshops within Katsina metropolis were collected. The samples were analyzed by selective enrichment technique, which resulted in the isolation of four bacterial species. The species were further subjected to the Vitek 2 compact microbiological system analysis. Cupriavidus pauculus, Pontoea spp, Proteus mirabilis 1 and Proteus mirabilis 2 were identified. Result from the present study showed that the bacteria could utilize phenol as their carbon source. Proteus mirabilis 1 and Proteus mirabilis 2 showed lower phenol degradation potential, under similar conditions. Cupriavidus pauculus and Pontoea sp. showed significant increases (p<0.05) in their optical densities. The optical density increment is strongly correlated with increase in colony forming units of the bacteria. This study further showed that the isolates could tolerate high phenol concentrations and may serve as strong putative isolates in bioremediation of phenol-contaminated sites.

Effects and modes of action of live yeasts in the rumen

Biologia ◽  
2006 ◽  
Vol 61 (6) ◽  
Author(s):  
Gérard Fonty ◽  
Frédérique Chaucheyras-Durand
Keyword(s):  
Feed Additives ◽  
Microbial Colonization ◽  
Yeast Cells ◽  
Rumen Microorganisms ◽  
Positive Effects ◽  
Modes Of Action ◽  
Oxygen Scavenging ◽  
Degrading Bacteria ◽  
Bacteria And Fungi ◽  
Set Up

AbstractLive yeasts (Saccharomyces cerevisiae) are more and more widely used as feed additives for ruminants. They are considered as allochtonous microorganisms in the rumen environment, however, distributed daily to dairy cows or beef cattle they can survive in the digestive tract and interact with autochtonous microbial populations. The positive effects of yeast cells have been mainly demonstrated on growth and activity of fibre-degrading bacteria and fungi, on stabilisation of rumen pH and prevention of lactate accumulation, on ruminal microbial colonization and on the set up of fermentative processes during the pre-weaning period. Modes of action of yeast probiotics depend on their viability and stability in the rumen ecosystem. Up to now, the main modes of action identified are the supply of growth factors to rumen microorganisms, oxygen scavenging inducing more favourable conditions for the anaerobic communities, and nutritional competition with autochtonous ruminal species.

scholarly journals Identification and characterisation of oil sludge degrading bacteria isolated from compost

2016 ◽  
Vol 42 (2) ◽  
pp. 67-77 ◽  
Author(s):  
Onyedikachi Ubani ◽  
Harrison Ifeanyichukwu Atagana ◽  
Mapitsi Silvester Thantsha ◽  
Adeleke Rasheed
Keyword(s):  
Phylogenetic Analyses ◽  
Oil Sludge ◽  
Bacterial Degradation ◽  
Rrna Gene ◽  
Molecular Weights ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic ◽  
Polymerase Chain ◽  
Bacteria And Fungi ◽  
The 16S Rrna Gene

AbstractCompounds present in oil sludge such as polycyclic aromatic hydrocarbons (PAHs) are known to be cytotoxic, mutagenic and potentially carcinogenic. Microorganisms including bacteria and fungi have been reported to degrade oil sludge components to innocuous compounds such as carbon dioxide, water and salts. In the present study, we isolated different bacteria with PAH-degrading capabilities from compost prepared from oil sludge and animal manures. These bacteria were isolated on a mineral base medium and mineral salt agar plates. A total of 31 morphologically distinct isolates were carefully selected from 5 different compost treatments for identification using polymerase chain reaction (PCR) of the 16S rRNA gene with specific primers (universal forward 16S-P1 PCR and reverse 16S-P2 PCR). The amplicons were sequenced and sequences were compared with the known nucleotides from the GenBank. The phylogenetic analyses of the isolates showed that they belong to 3 different clades; Firmicutes, Proteobacteria and Actinobacteria. These bacteria identified were closely related to the generaBacillus, Arthrobacter, Staphylococcus, Brevibacterium, Variovorax, Paenibacillus, Ralstonia and Geobacillus.The results showed thatBacillus species were predominant in all composts. Based on the results of the degradation of the PAHs in the composts and results of previous studies on bacterial degradation of hydrocarbons in oil, the characteristics of these bacterial isolates suggests that they may be responsible for the breakdown of PAHs of different molecular weights in the composts. Thus, they may be potentially useful for bioremediation of oil sludge during compost bioremediation.

CEYRANBATAN SU ANBARI SUYUNUN SANİTAR-KİMYƏVİ VƏ HİDROBİOLOJİ XÜSUSİYYƏTLƏRİ

2021 ◽  
Vol 1 (1) ◽  
pp. 89-95
Author(s):  
Ü.Ə Tağıyeva ◽  
X.Ə., Əliyeva ◽  
L.Ə., Əhmədova ◽  
, Ş.R. Əliyeva
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Water Reservoir ◽  
Ecological Condition ◽  
Practice Research ◽  
Biogenic Elements ◽  
Initial Product ◽  
Degrading Bacteria ◽  
Bacteria And Fungi ◽  
Practical Recommendations

Abstract. The article presents fragments of the research with some sanitarychemical and hydrobiological characteristics of the water of the Jeyranbatan reservoir, which has an exceptional role in the production of water by the population of baku and Absheron region. At the same time, the commented materials contain information on the hydro-ecological condition of the water of the Jeyranbatan reservoir, which provides drinking water to about 65% of the population of the region. During the assessment of the aquatic environment in the samples taken in the spring of 2020, important indicators such as initial product and destruction parameters were identified. The research was conducted with methods widely used in international practice. Research has been carried out to develop practical recommendations for the study of saprophytic bacteria, which are considered autochthonous microbiota, as well as a number of physiological groups of azotobacter, phenol-absorbing, denitrating, cellulose-degrading bacteria and fungi in water samples. Key words: Jeyranbatan water reservoir, biogenic elements, production, destruction, autochthon, microbiota.

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