Biomedical applications of muscle-derived stem cells: from bench to bedside

2020 ◽  
Vol 20 (11) ◽  
pp. 1361-1371
Author(s):  
Tetsuro Tamaki
Keyword(s):  
Stem Cells ◽  
Biomedical Applications

An Insight into DNA-free Reprogramming Approaches to Generate Integration-free Induced Pluripotent Stem Cells for Prospective Biomedical Applications

2018 ◽  
Vol 15 (2) ◽  
pp. 286-313 ◽  
Author(s):  
Manash P. Borgohain ◽  
Krishna Kumar Haridhasapavalan ◽  
Chandrima Dey ◽  
Poulomi Adhikari ◽  
Rajkumar P. Thummer
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Biomedical Applications ◽  
Induced Pluripotent ◽  
Insight Into

Biocompatible succinic acid-based polyesters for potential biomedical applications: fungal biofilm inhibition and mesenchymal stem cell growth

RSC Advances ◽  
2015 ◽  
Vol 5 (104) ◽  
pp. 85756-85766 ◽  
Author(s):  
E. Jäger ◽  
R. K. Donato ◽  
M. Perchacz ◽  
A. Jäger ◽  
F. Surman ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Cell Growth ◽  
Mesenchymal Stem Cell ◽  
Medical Devices ◽  
Biomedical Applications ◽  
Growth Promotion ◽  
Intrinsic Properties ◽  
Biofilm Inhibition

Poly(alkene succinates) are promising materials for specialized medical devices and tissue engineering, presenting intrinsic properties, such as; fungal biofilm inhibition, biocompatibility and stem cells controlled growth promotion.

scholarly journals Transfer of Synthetic Human Chromosome into Human Induced Pluripotent Stem Cells for Biomedical Applications

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 261 ◽  
Author(s):  
Sergey Sinenko ◽  
Elena Skvortsova ◽  
Mikhail Liskovykh ◽  
Sergey Ponomartsev ◽  
Andrey Kuzmin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Biomedical Applications ◽  
Fluorescent Protein ◽  
Leukemia Virus ◽  
Embryonic Stem ◽  
Induced Pluripotent Stem Cell ◽  
Human Artificial Chromosome ◽  
Human Escs ◽  
Human Ipscs ◽  
Induced Pluripotent

AlphoidtetO-type human artificial chromosome (HAC) has been recently synthetized as a novel class of gene delivery vectors for induced pluripotent stem cell (iPSC)-based tissue replacement therapeutic approach. This HAC vector was designed to deliver copies of genes into patients with genetic diseases caused by the loss of a particular gene function. The alphoidtetO-HAC vector has been successfully transferred into murine embryonic stem cells (ESCs) and maintained stably as an independent chromosome during the proliferation and differentiation of these cells. Human ESCs and iPSCs have significant differences in culturing conditions and pluripotency state in comparison with the murine naïve-type ESCs and iPSCs. To date, transferring alphoidtetO-HAC vector into human iPSCs (hiPSCs) remains a challenging task. In this study, we performed the microcell-mediated chromosome transfer (MMCT) of alphoidtetO-HAC expressing the green fluorescent protein into newly generated hiPSCs. We used a recently modified MMCT method that employs an envelope protein of amphotropic murine leukemia virus as a targeting cell fusion agent. Our data provide evidence that a totally artificial vector, alphoidtetO-HAC, can be transferred and maintained in human iPSCs as an independent autonomous chromosome without affecting pluripotent properties of the cells. These data also open new perspectives for implementing alphoidtetO-HAC as a gene therapy tool in future biomedical applications.

Biotechnological and biomedical applications of mesenchymal stem cells as a therapeutic system

2014 ◽  
Vol 44 (2) ◽  
pp. 559-570 ◽  
Author(s):  
Amirbahman Rahimzadeh ◽  
Fatemeh Sadat Tabatabaei Mirakabad ◽  
Aliakbar Movassaghpour ◽  
Karim Shamsasenjan ◽  
Saber Kariminekoo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Biomedical Applications ◽  
Therapeutic System

Transgenic perspectives in livestock science: a review

2004 ◽  
Vol 44 (11) ◽  
pp. 1113 ◽  
Author(s):  
M. B. Nottle ◽  
A. C. Boquest ◽  
S. J. Harrison ◽  
C. G. Grupen ◽  
R. A. Faast ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Biomedical Applications ◽  
Genetic Material ◽  
Embryonic Stem ◽  
Full Potential ◽  
Transgenic Technology ◽  
Artificial Chromosomes ◽  
Fetal Fibroblasts ◽  
The Future

The limitations of existing transgenic technology, the potential of cloning technology to overcome these, as well as technologies which may be available in the future for inserting new genetic material are discussed. Currently, transgenic livestock are produced by injecting hundreds to thousands of copies of a particular transgene into the pronucleus of a fertilised egg. This method suffers from a number of inherent limitations that prevent the full potential of this technology from being explored. Most of these limitations stem from the fact that it is impossible to control the site at which the transgene becomes inserted. Transgenic technology holds considerable promise for the livestock industries as well as having important biomedical applications. However, before any of these possibilities can be realised, technology is required whereby a single copy of a particular transgene can be inserted or ‘knocked in’ at a site that does not interfere with expression, as well as having the capacity to ‘knockout’ existing genes. This is possible in mice using a combination of homologous recombination and embryonic stem cell technologies. However, despite considerable effort worldwide, embryonic stem cells are yet to be isolated from any of the livestock species. The ability to clone these now means that somatic cells most notably fetal fibroblasts, can used for gene targeting purposes instead of embryonic stem cells. However, this method is not without its limitations and it is possible that more efficient methods will be developed in the future. In particular, the use of mammalian artificial chromosomes will extend this technology to allow combinations of transgenes as well as chromosomal segments to be incorporated, allowing us to explore the full potential of transgenic technology for agricultural as well as biomedical applications.

scholarly journals Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sepideh Yazdian Kashani ◽  
Mostafa Keshavarz Moraveji ◽  
Shahin Bonakdar
Keyword(s):  
Stem Cells ◽  
Microfluidic Device ◽  
Microfluidic Chip ◽  
Biomedical Applications ◽  
Chondrogenic Differentiation ◽  
Experimental Studies ◽  
Normal Behavior ◽  
A Cell ◽  
Computational Fluid Dynamics Cfd ◽  
Irregular Cell

AbstractIt has been proved that cell-imprinted substrates molded from template cells can be used for the re-culture of that cell while preserving its normal behavior or to differentiate the cultured stem cells into the template cell. In this study, a microfluidic device was presented to modify the previous irregular cell-imprinted substrate and increase imprinting efficiency by regular and objective cell culture. First, a cell-imprinted substrate from template cells was prepared using a microfluidic chip in a regular pattern. Another microfluidic chip with the same pattern was then aligned on the cell-imprinted substrate to create a chondrocyte-imprinted-based integrated microfluidic device. Computational fluid dynamics (CFD) simulations were used to obtain suitable conditions for injecting cells into the microfluidic chip before performing experimental evaluations. In this simulation, the effect of input flow rate, number per unit volume, and size of injected cells in two different chip sizes were examined on exerted shear stress and cell trajectories. This numerical simulation was first validated with experiments with cell lines. Finally, chondrocyte was used as template cell to evaluate the chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) in the chondrocyte-imprinted-based integrated microfluidic device. ADSCs were positioned precisely on the chondrocyte patterns, and without using any chemical growth factor, their fibroblast-like morphology was modified to the spherical morphology of chondrocytes after 14 days of culture. Both immunostaining and gene expression analysis showed improvement in chondrogenic differentiation compared to traditional imprinting methods. This study demonstrated the effectiveness of cell-imprinted-based integrated microfluidic devices for biomedical applications.

scholarly journals Polymeric Gelatin Scaffolds Affect Mesenchymal Stem Cell Differentiation and Its Diverse Applications in Tissue Engineering

2020 ◽  
Vol 21 (22) ◽  
pp. 8632
Author(s):  
Chia-Yu Wang ◽  
Po-Da Hong ◽  
Ding-Han Wang ◽  
Juin-Hong Cherng ◽  
Shu-Jen Chang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Defect ◽  
Biomedical Applications ◽  
Stem Cell Differentiation ◽  
Osteogenic Potential ◽  
Calvarial Bone ◽  
Suitable Material ◽  
Mesenchymal Stem Cell Differentiation

Studies using polymeric scaffolds for various biomedical applications, such as tissue engineering, implants and medical substitutes, and drug delivery systems, have attempted to identify suitable material for tissue regeneration. This study aimed to investigate the biocompatibility and effectiveness of a gelatin scaffold seeded with human adipose stem cells (hASCs), including physical characteristics, multilineage differentiation in vitro, and osteogenic potential, in a rat model of a calvarial bone defect and to optimize its design. This functionalized scaffold comprised gelatin-hASCs layers to improve their efficacy in various biomedical applications. The gelatin scaffold exhibited excellent biocompatibility in vitro after two weeks of implantation. Furthermore, the gelatin scaffold supported and specifically regulated the proliferation and osteogenic and chondrogenic differentiation of hASCs, respectively. After 12 weeks of implantation, upon treatment with the gelatin-hASCs scaffold, the calvarial bone harboring the critical defect regenerated better and displayed greater osteogenic potential without any damage to the surrounding tissues compared to the untreated bone defect. These findings suggest that the present gelatin scaffold is a good potential carrier for stem cells in various tissue engineering applications.

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