scholarly journals iPS Cells: Born-Again Stem Cells for Biomedical Applications

10.5772/19440 ◽  
2012 ◽  
Author(s):  
Ambrose Jon ◽  
Vimal Selvaraj
Keyword(s):  
Stem Cells ◽  
Biomedical Applications ◽  
Ips Cells ◽  
Born Again

An Insight into DNA-free Reprogramming Approaches to Generate Integration-free Induced Pluripotent Stem Cells for Prospective Biomedical Applications

2018 ◽  
Vol 15 (2) ◽  
pp. 286-313 ◽  
Author(s):  
Manash P. Borgohain ◽  
Krishna Kumar Haridhasapavalan ◽  
Chandrima Dey ◽  
Poulomi Adhikari ◽  
Rajkumar P. Thummer
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Biomedical Applications ◽  
Induced Pluripotent ◽  
Insight Into

Biocompatible succinic acid-based polyesters for potential biomedical applications: fungal biofilm inhibition and mesenchymal stem cell growth

RSC Advances ◽  
2015 ◽  
Vol 5 (104) ◽  
pp. 85756-85766 ◽  
Author(s):  
E. Jäger ◽  
R. K. Donato ◽  
M. Perchacz ◽  
A. Jäger ◽  
F. Surman ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Cell Growth ◽  
Mesenchymal Stem Cell ◽  
Medical Devices ◽  
Biomedical Applications ◽  
Growth Promotion ◽  
Intrinsic Properties ◽  
Biofilm Inhibition

Poly(alkene succinates) are promising materials for specialized medical devices and tissue engineering, presenting intrinsic properties, such as; fungal biofilm inhibition, biocompatibility and stem cells controlled growth promotion.

Abstract 340: From Stem Cells to Cardiomyocytes: HDAC1 Induces Cardiovascular Differentiation by Regulating SOX17-BMP2 Expression in Early Development.

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Eneda Hoxha ◽  
Erin Lambers ◽  
Veronica Ramirez ◽  
Prasanna Krishnamurthy ◽  
Suresh Verma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Molecular Mechanisms ◽  
Es Cells ◽  
Ips Cells ◽  
Full Potential ◽  
Pluripotent Cells ◽  
Differentiation Potential ◽  
Histone Deacetylase 1 ◽  
Specific Differentiation ◽  
Induced Pluripotent

Cardiomyocytes derived from embryonic and induced pluripotent stem cells (ES/iPS) provide an excellent source for cell replacement therapies following myocardial ischemia. However, some of the obstacles in the realization of the full potential of iPS/ES cells arise from incomplete and poorly understood molecular mechanisms and epigenetic modifications that govern their cardiovascular specific differentiation. We identified Histone Deacetylase 1 (HDAC1) as a crucial regulator in early differentiation of mES and iPS cells. We propose a novel pathway in which HDAC1 regulates cardiovascular differentiation by regulating SOX17 which in turn regulates BMP2 signaling in differentiating pluripotent cells. Utilizing stable HDAC1 knock-down (HDAC1-KD) cell lines, we report an essential role for HDAC1 in deacetylating regulatory regions of pluripotency-associated genes during early cardiovascular differentiation. HDAC1-KD cells show severely repressed cardiomyocyte differentiation potential. We propose a novel HDAC1-BMP2-SOX17 dependent pathway through which deacetylation of pluripotency associated genes leads to their suppression and allows for early cardiovascular-associated genes to be expressed and differentiation to occur. Furthermore, we show that HDAC1 affects DNA methylation both during pluripotency and differentiation and plays a crucial, non-redundant role in cardiovascular specific differentiation and cardiomyocyte maturation. Our data elucidates important differences between ES and iPS HDAC1-KD cells that affect their ability to differentiate into cardiovascular lineages. As varying levels of chromatin modifying enzymes are likely to exist in patient derived iPS cells, understanding the molecular circuitry of these enzymes in ES and iPS cells is critical for their potential therapeutic applications in regenerative medicine. Further research in the molecular mechanisms involved in this process will greatly aid our understanding of the epigenetic circuitry of pluripotency and differentiation in pluripotent cells.

scholarly journals Transfer of Synthetic Human Chromosome into Human Induced Pluripotent Stem Cells for Biomedical Applications

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 261 ◽  
Author(s):  
Sergey Sinenko ◽  
Elena Skvortsova ◽  
Mikhail Liskovykh ◽  
Sergey Ponomartsev ◽  
Andrey Kuzmin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Biomedical Applications ◽  
Fluorescent Protein ◽  
Leukemia Virus ◽  
Embryonic Stem ◽  
Induced Pluripotent Stem Cell ◽  
Human Artificial Chromosome ◽  
Human Escs ◽  
Human Ipscs ◽  
Induced Pluripotent

AlphoidtetO-type human artificial chromosome (HAC) has been recently synthetized as a novel class of gene delivery vectors for induced pluripotent stem cell (iPSC)-based tissue replacement therapeutic approach. This HAC vector was designed to deliver copies of genes into patients with genetic diseases caused by the loss of a particular gene function. The alphoidtetO-HAC vector has been successfully transferred into murine embryonic stem cells (ESCs) and maintained stably as an independent chromosome during the proliferation and differentiation of these cells. Human ESCs and iPSCs have significant differences in culturing conditions and pluripotency state in comparison with the murine naïve-type ESCs and iPSCs. To date, transferring alphoidtetO-HAC vector into human iPSCs (hiPSCs) remains a challenging task. In this study, we performed the microcell-mediated chromosome transfer (MMCT) of alphoidtetO-HAC expressing the green fluorescent protein into newly generated hiPSCs. We used a recently modified MMCT method that employs an envelope protein of amphotropic murine leukemia virus as a targeting cell fusion agent. Our data provide evidence that a totally artificial vector, alphoidtetO-HAC, can be transferred and maintained in human iPSCs as an independent autonomous chromosome without affecting pluripotent properties of the cells. These data also open new perspectives for implementing alphoidtetO-HAC as a gene therapy tool in future biomedical applications.

Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells

2011 ◽  
Vol 71 ◽  
pp. e332
Author(s):  
Hiroko Shimada ◽  
Yohei Okada ◽  
Ikuo Tomioka ◽  
Erika Sasaki ◽  
Masaya Nakamura ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Es Cells ◽  
Common Marmoset ◽  
Ips Cells

Biotechnological and biomedical applications of mesenchymal stem cells as a therapeutic system

2014 ◽  
Vol 44 (2) ◽  
pp. 559-570 ◽  
Author(s):  
Amirbahman Rahimzadeh ◽  
Fatemeh Sadat Tabatabaei Mirakabad ◽  
Aliakbar Movassaghpour ◽  
Karim Shamsasenjan ◽  
Saber Kariminekoo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Biomedical Applications ◽  
Therapeutic System

scholarly journals Signaling Inhibitors Accelerate the Conversion of mouse iPS Cells into Cancer Stem Cells in the Tumor Microenvironment

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Juan Du ◽  
Yanning Xu ◽  
Saki Sasada ◽  
Aung Ko Ko Oo ◽  
Ghmkin Hassan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tumor Microenvironment ◽  
Cancer Stem Cells ◽  
Ips Cells ◽  
Signaling Inhibitors

scholarly journals Molecular and Functional Verification of Wharton’s Jelly Mesenchymal Stem Cells (WJ-MSCs) Pluripotency

2019 ◽  
Vol 20 (8) ◽  
pp. 1807 ◽  
Author(s):  
Aleksandra Musiał-Wysocka ◽  
Marta Kot ◽  
Maciej Sułkowski ◽  
Bogna Badyra ◽  
Marcin Majka
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ips Cells ◽  
Biological Properties ◽  
Wharton’S Jelly ◽  
Functional Verification ◽  
High Plasticity ◽  
Wharton's Jelly ◽  
Pluripotency Markers

The properties of mesenchymal stem cells (MSCs), especially their self-renewal and ability to differentiate into different cell lines, are widely discussed. Considering the fact that MSCs isolated from perinatal tissues reveal higher differentiation capacity than most adult MSCs, we examined mesenchymal stem cells isolated from Wharton’s jelly of umbilical cord (WJ-MSCs) in terms of pluripotency markers expression. Our studies showed that WJ-MSCs express some pluripotency markers—such as NANOG, OCT-4, and SSEA-4—but in comparison to iPS cells expression level is significantly lower. The level of expression can be raised under hypoxic conditions. Despite their high proliferation potential and ability to differentiate into different cells type, WJ-MSCs do not form tumors in vivo, the major caveat of iPS cells. Owing to their biological properties, high plasticity, proliferation capacity, and ease of isolation and culture, WJ-MSCs are turning out to be a promising tool of modern regenerative medicine.

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