Polyphenolic profile, antibacterial activity and brine shrimp toxicity of leaf extracts from six Tunisian spontaneous species

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Hajer Tlili ◽  
Andreana Marino ◽  
Giovanna Ginestra ◽  
Francesco Cacciola ◽  
Luigi Mondello ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Brine Shrimp ◽  
Leaf Extracts ◽  
Brine Shrimp Toxicity ◽  
Polyphenolic Profile

scholarly journals Antioxidant, Antibacterial activity and Brine shrimp toxicity test of some Mountainous Lichens from Nepal

2012 ◽  
Vol 45 (4) ◽  
pp. 387-391 ◽  
Author(s):  
Babita Paudel ◽  
Hari Datta Bhattarai ◽  
Durga Prasad Pandey ◽  
Jae Seoun Hur ◽  
Soon Gyu Hong ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Brine Shrimp Toxicity

The in vitro Antibacterial Activity and Brine Shrimp Toxicity of Manihot esculenta var. Sri Pontian (Euphorbiacea) Extracts

2006 ◽  
Vol 2 (2) ◽  
pp. 216-220 ◽  
Author(s):  
Z.A. Zakaria ◽  
H.M. Khairi . ◽  
M.N. Somchit . ◽  
M.R. Sulaiman . ◽  
A.M. Mat Jais . ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Manihot Esculenta ◽  
Brine Shrimp ◽  
In Vitro Antibacterial Activity ◽  
Brine Shrimp Toxicity

Antibacterial Activity against Clinical Isolates of Salmonella enterica Serovar Paratyphi and Brine Shrimp Lethality Assay of Trichilia megalanthaHarms and Trichilia welwitschii C. DC

2020 ◽  
Vol 36 (1) ◽  
pp. 11-18
Author(s):  
Philip A. Idowu ◽  
Tajudeen A. Adegbenle
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Salmonella Enterica ◽  
Brine Shrimp ◽  
Antimicrobial Agents ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay

Increasing resistance of typhoidal Salmonella enterica to conventional antibiotics has caused more cases of typhoid, therapeutic failure, morbidity and mortality; creating the need to search for new and effective antimicrobial agents from medicinal plants. The present study aimed to detect antisalmonella activity and cytotoxicity (safety) status of the stem bark and leaves of two Nigerian medicinal plants, Trichilia megalantha and Trichilia welwitschii. Nine clinical isolates of Salmonella paratyphi, whose antibiogram were determined by Kirby-Bauer disc diffusion method were used. Antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on the clinical isolates were done by agar cup diffusion and agar dilution methods, respectively. Acute toxicity of the extracts was determined using brine shrimp lethality assay (BSLA). All the nine isolates of Salmonella paratyphi were resistant to β-lactam antibiotics (augmentin, cefuroxime, ceftazidime and ampicillin) but susceptible to fluoroquinolones (ciprofloxacin, ofloxacin), nitrofurantoin and gentamicin. The crude extracts of the two plants elicited activity against the nine clinical isolates with the bark extracts being more active than the leaf extracts. T. welwitschii was slightly less active than T. megalantha. The MIC and MBC ranged 1.25 - 5.0 mg/ ml and 2.5 - 10 mg/ml, respectively for the plants. The antisalmonella activity of methanolic extracts of both plants were found to be less than that of ciprofloxacin and ofloxacin. With modal cytotoxicity values of 400 - 500 μg/ml, the plant parts were considered nontoxic. Therefore, T. megalantha and T. welwitschii could provide a potential source of antibacterial agent(s) for the treatment of Salmonella paratyphoid infections. Keywords: Trichilia megalantha; Trichilia welwitschii, antisalmonella; cytotoxicity; Salmonella paratyphi 

scholarly journals Azadirachta indica and Terminalia arjuna Leaf Extracts Induce Death of Bacterial Cells Involving Aggregation of Proteins

1970 ◽  
Vol 25 (2) ◽  
pp. 115-121
Author(s):  
Miraj Kobad Chowdhury ◽  
Nazmul Ahsan ◽  
Anwarul Azim Akhand
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
Azadirachta Indica ◽  
Brine Shrimp ◽  
Cellular Proteins ◽  
Terminalia Arjuna ◽  
Bacterial Cells ◽  
Leaf Extracts ◽  
Lc50 Value ◽  
Therapeutic Value

Different parts of various plants or their extracts have been used to treat a number of diseases from the ancient time due to their therapeutic value. However, their mechanism of actions remains mostly undiscovered. In this study, brine shrimp (Artemia salina) lethality and antibacterial activity of Azadirachta indica and Terminalia arjuna ethanolic leaf extracts were investigated. A. indica extract showed greater brine shrimp cytotoxicity with lethal concentration 50 (LC50) value of 36.813 mg/ml, whereas the LC50 value of T. arjuna extract was 44.157 mg/ml. Antimicrobial activity of these extracts was assayed by agar-well diffusion method and it was found that both of the extracts were effective against both Gram-positive and Gram-negative bacteria. Later, we found that the cellular proteins isolated from Shigella dysenteriae, which were treated with both of the plant extracts, were aggregated. This aggregation of proteins was demonstrated by detection of protein bands at the upper portion of both of the stacking and separating gels. The extracts-mediated aggregation of cellular proteins might be responsible for the cytotoxic effect that ultimately caused death of the bacterial cells. All of the above results suggest that both of the plant extracts have potential bioactivities that may have therapeutic value. These findings may lead us to develop new effective medicine in future. Key words: Azadirachta indica; Terminalia arjuna; Brine shrimp; Antibacterial activity; Protein aggregationDOI: http://dx.doi.org/10.3329/bjm.v25i2.4873 Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 115-121

Antibacterial activity of Matricaria chamomilla flower and leaf extracts

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
BF Navarro ◽  
M del Carmen Ortiz García ◽  
BN Marin ◽  
ML Rodríguez ◽  
AV Hernández
Keyword(s):  
Antibacterial Activity ◽  
Matricaria Chamomilla ◽  
Leaf Extracts

scholarly journals Antibiotic sensitivity and in vitro antimicrobial activity of plant extracts to pseudomonas fluorescens isolates collected from diseased fish

1970 ◽  
Vol 1 (4) ◽  
pp. 82-88 ◽  
Author(s):  
MJ Foysal ◽  
MM Rahman ◽  
M Alam
Keyword(s):  
Antibacterial Activity ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Leaf Extracts ◽  
Sensitivity Pattern ◽  
Medicinal Plant Extracts

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88

scholarly journals Study on Phytochemical, Antibacterial, Antioxidant and Toxicity Profile of Viscum album Linn Associated with Acacia catechu

2015 ◽  
Vol 3 (1) ◽  
pp. 60-65
Author(s):  
Meena Kusi ◽  
Kanti Shrestha ◽  
Rajani Malla
Keyword(s):  
Brine Shrimp ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Viscum Album ◽  
Biologically Active ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Percolation Method ◽  
Brine Shrimp Toxicity ◽  
Acacia Catechu

This study focuses on antibacterial, antioxidant and toxic potentials of Viscum album Linn, commonly known as European mistletoe associated with Acacia catechu (Khayer in Nepali). Methanol extract of the aerial parts of the Mistletoe was prepared by cold percolation method. The resulting extract was simultaneously subjected to phytochemical screening; anti-microbial activity; anti-oxidant potential and Brine shrimp toxicity test. The major biologically active phyto-constituents observed were alkaloids, glycosides, saponins, polyphenols, flavonoids, tannins, terpenoids and cardiac glycosides. Upon antibacterial activity screening, the plant extract was found to be highly effective against Pseudomonas aeruginosa with the zone of inhibition 16±1mm compared to 17±1mm of chloramphenicol (50 mcg). The antioxidant activity as EC50 value by DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity was found to be 1.58 mg/ml while the ferric reducing capacity was measured to be 282.83±19.55 mg FeSO4.7H2O eqvt/g dry wt. of the extract during Ferric Ion Reducing Antioxidant Power (FRAP) Assay. The LC50 value for Brine Shrimp Toxicity Assay was found to be 31.62 ppm. This study shows the medicinal value of the mistletoe associated with Acacia catechu. Further meticulous analysis of this plant might lead to identification of active biomolecules effective as drugs for various ailmentsNepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 60-65

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals Bioactivity of extracts of Centaurea polyclada dc. (Asteraceae)

2009 ◽  
Vol 61 (3) ◽  
pp. 447-452 ◽  
Author(s):  
Melanie Granger ◽  
Emilie Samson ◽  
Severine Sauvage ◽  
Anisha Majumdar ◽  
Poonam Nigam ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Solid Phase Extraction ◽  
Brine Shrimp ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase Extraction ◽  
Brine Shrimp Lethality Assay

The free radical-scavenging property, antibacterial activity, and brine shrimp toxicity of n-hexane, dichloromethane (DCM), and methanol (MeOH) extracts of Centaurea polyclada, an endemic Turkish species, were assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtiter plate-based assay, and the brine shrimp lethality assay, respectively. The DCM and MeOH extracts of C. polyclada exhibited free radical-scavenging ability with RC50 values 1.17 and 0.015 mg/mL, respectively. Among solid-phase extraction fractions of the MeOH extract, the fraction eluted with 60% MeOH in water demonstrated the highest level of free radical-scavenging activity (RC50 = 0.016 mg/mL). Only the DCM extract showed considerable antibacterial activity against all nine test strains except Escherichia coli, with MIC ranging from 1.25 to 2.50 mg/mL. This antibacterial activity pattern was also observed with solid-phase extraction fractions of the DCM extract with varied potencies. None of the extracts showed any significant toxicity towards brine shrimps (LD50 = >1.00 mg/mL).

Sign in / Sign up

Export Citation Format

Share Document