Antioxidant, Antibacterial activity and Brine shrimp toxicity test of some Mountainous Lichens from Nepal

The present study determined the antifungal activity of the root of extracts Argemone mexicana against 7 fungi using dichloromethane and methanol as solvent. Among these pathogens, 3 were dermatophytes and the rest 4 were nondermatophytes. The dichloromethane extract of Argemone mexicana exhibited moderate to good activity at concentration of 200 ?g/disc but at concentration of 50 ?g/disc, the same extract showed no activity against the tested fungi. The methanol extract were also inactive against the tested pathogens. In brine shrimp toxicity test, it was observed that LC50 value of the dichloromethane extract of Argemone mexicana was 22.35 ?g/ml. From these findings it is indicative that dichloromethane extract of Argemone mexicana is biologically active and may have antifungal principles that could be useful in fungal diseases.KYAMC Journal Vol. 4, No.-2, Jan 2014, Page 402-405

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Study on Phytochemical, Antibacterial, Antioxidant and Toxicity Profile of Viscum album Linn Associated with Acacia catechu

Nepal Journal of Biotechnology ◽

10.3126/njb.v3i1.14234 ◽

2015 ◽

Vol 3 (1) ◽

pp. 60-65

Author(s):

Meena Kusi ◽

Kanti Shrestha ◽

Rajani Malla

Keyword(s):

Brine Shrimp ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Viscum Album ◽

Biologically Active ◽

Frap Assay ◽

Antioxidant Power ◽

Percolation Method ◽

Brine Shrimp Toxicity ◽

Acacia Catechu

This study focuses on antibacterial, antioxidant and toxic potentials of Viscum album Linn, commonly known as European mistletoe associated with Acacia catechu (Khayer in Nepali). Methanol extract of the aerial parts of the Mistletoe was prepared by cold percolation method. The resulting extract was simultaneously subjected to phytochemical screening; anti-microbial activity; anti-oxidant potential and Brine shrimp toxicity test. The major biologically active phyto-constituents observed were alkaloids, glycosides, saponins, polyphenols, flavonoids, tannins, terpenoids and cardiac glycosides. Upon antibacterial activity screening, the plant extract was found to be highly effective against Pseudomonas aeruginosa with the zone of inhibition 16±1mm compared to 17±1mm of chloramphenicol (50 mcg). The antioxidant activity as EC50 value by DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity was found to be 1.58 mg/ml while the ferric reducing capacity was measured to be 282.83±19.55 mg FeSO4.7H2O eqvt/g dry wt. of the extract during Ferric Ion Reducing Antioxidant Power (FRAP) Assay. The LC50 value for Brine Shrimp Toxicity Assay was found to be 31.62 ppm. This study shows the medicinal value of the mistletoe associated with Acacia catechu. Further meticulous analysis of this plant might lead to identification of active biomolecules effective as drugs for various ailmentsNepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 60-65

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Bioactivity of extracts of Centaurea polyclada dc. (Asteraceae)

Archives of Biological Sciences ◽

10.2298/abs0903447g ◽

2009 ◽

Vol 61 (3) ◽

pp. 447-452 ◽

Cited By ~ 6

Author(s):

Melanie Granger ◽

Emilie Samson ◽

Severine Sauvage ◽

Anisha Majumdar ◽

Poonam Nigam ◽

...

Keyword(s):

Antibacterial Activity ◽

Free Radical ◽

Solid Phase Extraction ◽

Brine Shrimp ◽

Solid Phase ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Phase Extraction ◽

Brine Shrimp Lethality Assay

The free radical-scavenging property, antibacterial activity, and brine shrimp toxicity of n-hexane, dichloromethane (DCM), and methanol (MeOH) extracts of Centaurea polyclada, an endemic Turkish species, were assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtiter plate-based assay, and the brine shrimp lethality assay, respectively. The DCM and MeOH extracts of C. polyclada exhibited free radical-scavenging ability with RC50 values 1.17 and 0.015 mg/mL, respectively. Among solid-phase extraction fractions of the MeOH extract, the fraction eluted with 60% MeOH in water demonstrated the highest level of free radical-scavenging activity (RC50 = 0.016 mg/mL). Only the DCM extract showed considerable antibacterial activity against all nine test strains except Escherichia coli, with MIC ranging from 1.25 to 2.50 mg/mL. This antibacterial activity pattern was also observed with solid-phase extraction fractions of the DCM extract with varied potencies. None of the extracts showed any significant toxicity towards brine shrimps (LD50 = >1.00 mg/mL).

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Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract

Elkawnie ◽

10.22373/ekw.v6i1.5732 ◽

2020 ◽

Vol 6 (1) ◽

pp. 67

Author(s):

Robby Gus Mahardika ◽

Occa Roanisca ◽

Fajar Indah Puspita Sari

Keyword(s):

Toxicity Test ◽

Brine Shrimp ◽

Leaf Extract ◽

Positive Control ◽

Acetone Extract ◽

Antidiabetic Activity ◽

Brine Shrimp Lethality ◽

Lc50 Value ◽

Ic50 Value ◽

Ft Ir

Abstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract possessed antidiabetic activity with an IC50 value of 8.83 ± 0.31 (μg/mL). This value is not much different from the positive control of quercetin which has an IC50 value of 6.04 ± 0.14 (μg/mL). The characteristics of the FT-IR spectrum of acetone extract showed that Tristaniopsis merguensis leaf has the groups Ar-OH (phenolic), -OH (hydroxyl), C=O (ketone) and C=C (aromatic). Based on the toxicity test, the Tristaniopsis merguensis leaf acetone extract has an LC50 value of 959.25 ppm which means that the acetone extract is toxic. Therefore, the acetone extract of Tristaniopsis merguensis might be the potential agent of antidiabetic.Abstrak : Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan toksisitas dari ekstrak aseton daun Tristaniopsis merguensis Griff. Uji aktivitas antidiabetes ditentukan berdasarkan metode inhibisi enzim α-glucosidase, sedangkan toksisitas ditentukan berdasarkan metode Brine Shrimp Lethality Test (BSLT). Ekstrak aseton memiliki aktivitas antidiabetes dengan nilai IC50 8,83 ± 0,31 (μg/mL). Nilai ini tidak jauh berbeda dengan kontrol positif quersetin yang memiliki nilai IC50 6,04 ± 0,14 (μg/mL). Karakteristik spektrum FT-IR ekstrak aseton menunjukkan bahwa daun Tristaniopsis merguensis memiliki gugus fungsi Ar-OH (fenolik), -OH (hidroksil), C=O (keton) dan C=C (aromatik). Berdasarkan uji toksisitas, ekstrak aseton daun Tristaniopsis merguensis memiliki nilai LC50 sebesar 959,25 ppm yang berarti bahwa ekstrak aseton bersifat toksik. Oleh karena itu, ekstrak aseton dari Tristaniopsis merguensis berpotensi untuk dijadikan agen antidiabetes.

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UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.7.3.2019.26017 ◽

2019 ◽

Vol 7 (3) ◽

pp. 265

Author(s):

Faradila Y. Karim ◽

Nickson J Kawung ◽

Billy Th. Wagey

Keyword(s):

Cytotoxic Activity ◽

Toxicity Test ◽

Brine Shrimp ◽

Bioactive Compound ◽

Artemia Salina ◽

Probit Analysis ◽

Thalassia Hemprichii ◽

Brine Shrimp Lethality ◽

A Value ◽

Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)

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The potential biopesticide toxicity test of Ipomoea batatas (L.) Lam (Purple Sweet Potato leaf extract) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test Method

International Journal of Scientific and Research Publications (IJSRP) ◽

10.29322/ijsrp.10.08.2020.p10428 ◽

2020 ◽

Vol 10 (8) ◽

pp. 212-217

Author(s):

Onik Anggita Sari ◽

Slamet Isworo

Keyword(s):

Sweet Potato ◽

Toxicity Test ◽

Brine Shrimp ◽

Ipomoea Batatas ◽

Leaf Extract ◽

Artemia Salina ◽

Test Method ◽

Potato Leaf ◽

Brine Shrimp Lethality ◽

Purple Sweet Potato

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Brine Shrimp Toxicity of Leaf and Seed Extracts of Cassia alata Linn. and Their Antibacterial Potency

Journal of Medical Sciences(Faisalabad) ◽

10.3923/jms.2004.188.193 ◽

2004 ◽

Vol 4 (3) ◽

pp. 188-193 ◽

Cited By ~ 16

Author(s):

M.A. Awal ◽

Ainun Nahar . ◽

M. Shamim Hossain . ◽

M.A. Bari . ◽

M. Rahman . ◽

...

Keyword(s):

Brine Shrimp ◽

Cassia Alata ◽

Brine Shrimp Toxicity ◽

Seed Extracts

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Screening of Plants Used as Traditional Anticancer Remedies in Mkuranga and Same Districts, Tanzania, Using Brine Shrimp Toxicity Bioassay

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/3034612 ◽

2018 ◽

Vol 2018 ◽

pp. 1-16 ◽

Cited By ~ 3

Author(s):

Daniel Z. Matata ◽

Olipa D. Ngassapa ◽

Francis Machumi ◽

Mainen J. Moshi

Keyword(s):

Plant Species ◽

Brine Shrimp ◽

Health Care Services ◽

Literature Search ◽

Strong Support ◽

Preliminary Evaluation ◽

Contributing Factors ◽

Anticancer Compounds ◽

Care Services ◽

Brine Shrimp Toxicity

Background.Inadequate specialized cancer hospitals and high costs are contributing factors that delay cancer patients from accessing health care services in Tanzania. Consequently, majority of patients are first seen by Traditional Health Practitioners (THPs) before they access specialized services. This study presents ethnomedical information and preliminary evaluation of 25 plant species claimed by THPs in Mkuranga and Same districts of Tanzania on use for treatment of cancer. Literature search and laboratory investigation results are presented to support evaluation.Methods. This study was a single disease ethnomedical enquiry focusing on plants being used for cancer treatment.Face-to-face interviews and questionnaires were administered toeight (8) THPsin Mkuranga and Same districts on the claimed plants and their use for management of cancer. Plants were selected based on being frequently mentioned and emphasis given by THPs. Literature search and brine shrimp toxicity (BST) of methanol : dichloromethane (1:1) extracts was used as surrogates to evaluate strength of the claims.Results.This study reports 25 plant species used by the THPs in two districts of Tanzania. Eight plants (32%) have been reported in the literature to have activity against cancer cells. BST results revealed, 14 (56%) plants exhibited high toxicity against brine shrimps. The most active plants includedCroton pseudopulchellusPax (LC504.2μg/ml),Dalbergia melanoxylonGuill. & Perr. (LC506.8μg/ml),Loranthus micranthusLinn (LC504.0μg/ml),Ochna mossambicensisKlotzsch (LC503.3μg/ml), andSpirostachys africanaSond. (LC504.4μg/ml); their toxicity was comparable to that ofCatharanthus roseus(L) G. Don. (LC506.7μg/ml), an established source of anticancer compounds. Nine other plants had LC50values between (19.8 and 71.6)μg/ml, indicating also potential to yield anticancer.Conclusion.Literature search and BST results provide a strong support of the potential of the claimed plants to yield active anticancer compounds.

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