Correlation Between Antioxidant Activity and Hepatoprotective Effect of a Vegetal Bioproduct

The purpose of this study was to evaluate the antioxidant activity and hepatoprotective effect of ethanol extracts of Actinidia rubricaulis (AR) on chronic liver injury induced by carbon tetrachloride ( CCl4) in rats. CCl4(20%, 0.5 ml/rat) was given twice a week for 8 weeks, and animals received AR throughout the entire experimental period. AR reduced the elevated levels of serum glutamate-oxalate-transaminase (sGOT) and glutamate-pyruvate-transaminase (sGPT) caused by CCl4at weeks 1,3,6, and 8. The biochemical data were consistent with those of the histological observations. The AR extract recovered the CCl4-induced liver injury and showed antioxidant effect in assays of antioxidant enzyme activity, such as SOD, GSH-Px and GSH-Rd. Based on these results, we suggest that the hepatoprotective effect of the AR is related to its antioxidant activity.

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Hepatoprotective Effect of Polyphenol-Enriched Fraction from Folium Microcos on Oxidative Stress and Apoptosis in Acetaminophen-Induced Liver Injury in Mice

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/3631565 ◽

2017 ◽

Vol 2017 ◽

pp. 1-14 ◽

Cited By ~ 17

Author(s):

Hongtan Wu ◽

Gang Zhang ◽

Lisen Huang ◽

Haiyue Pang ◽

Na Zhang ◽

...

Keyword(s):

Antioxidant Activity ◽

Liver Injury ◽

Target Genes ◽

Therapeutic Potential ◽

Radical Scavenging ◽

Hepatoprotective Effect ◽

Nuclear Accumulation ◽

Related Factor ◽

Hepatic Expression ◽

Phenolic Components

Folium Microcos (FM), the leaves of Microcos paniculata L., shows various biological functions including antioxidant activity and α-glucosidase inhibitory effect. However, its therapeutic potential in acute liver injury is still unknown. This study investigated the hepatoprotective effect and underlying mechanisms of the polyphenol-enriched fraction (FMF) from Folium Microcos. FMF exhibited strong free radical scavenging activities and prevented HepG2/Hepa1–6 cells from hydrogen peroxide- (H2O2-) induced ROS production and apoptosis in vitro. Antioxidant activity and cytoprotective effects were further verified by alleviating APAP-induced hepatotoxicity in mice. Western blot analysis revealed that FMF pretreatment significantly abrogated APAP-mediated phosphorylation of MAPKs, activation of proapoptotic protein caspase-3/9 and Bax, and restored expression of antiapoptotic protein Bcl2. APAP-intoxicated mice pretreated with FMF showed increased nuclear accumulation of nuclear factor erythroid 2-related factor (Nrf2) and elevated hepatic expression of its target genes, NAD(P)H:quinine oxidoreductase 1 (NQO1) and hemeoxygenase-1(HO-1). HPLC analysis revealed the four predominantly phenolic compounds present in FMF: narcissin, isorhamnetin-3-O-β-D-glucoside, isovitexin, and vitexin. Consequently, these findings indicate that FMF possesses a hepatoprotective effect against APAP-induced hepatotoxicity mainly through dual modification of ROS/MAPKs/apoptosis axis and Nrf2-mediated antioxidant response, which may be attributed to the strong antioxidant activity of phenolic components.

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Antioxidant Activity and Hepatoprotective Effect of Exopolysaccharides From Cultivated Ophiocordyceps Sinensis Against CCl4-Induced Liver Damages

Natural Product Communications ◽

10.1177/1934578x21997670 ◽

2021 ◽

Vol 16 (2) ◽

pp. 1934578X2199767

Author(s):

Quang-Vinh Nguyen ◽

Thi-Tuoi Vu ◽

Minh-Trang Tran ◽

Phuong Tham Ho Thi ◽

Huynh Thu ◽

...

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Positive Control ◽

Hepatoprotective Effect ◽

Protective Effects ◽

Bioactive Components ◽

Health Food ◽

Ophiocordyceps Sinensis ◽

Oxidative Effect

Cordyceps is a well-known and valuable fungal Chinese medicine and health food. The polysaccharides from C. sinensis have been reported as the main bioactive components, which possess antioxidant, anti-aging, and liver protective activities. This study aimed to investigate the in vitro antioxidative and protective effects of exopolysaccharides (EPS) from cultivated Ophiocordyceps sinensis (O. sinensis) on rats with liver damage induced by CCl4. The results indicated that cultivated O. sinensis EPS possess moderate ABTS and hydroxyl radical scavenging abilities with IC50 values of 2.00 and 3.78 mg/mL, respectively. EPS and the positive control (silymarin) could also protect rat liver from the oxidative effect induced by CCl4 via increasing GSH levels, reducing MAD levels and decreasing serum AST and ALT activities. Moreover, the changes in histopathological liver tissue showed that EPS significantly reduced the damage induced by CCl4 in the liver. The findings suggest that EPS possesses a significant hepatoprotective effect against hepatotoxicity induced by CCl4 in rats.

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Antioxidant activity and hepatoprotective effect of 10 medicinal herbs on CCl4-induced liver injury in mice

World Journal of Gastroenterology ◽

10.3748/wjg.v26.i37.5629 ◽

2020 ◽

Vol 26 (37) ◽

pp. 5629-5645

Author(s):

Xiao Meng ◽

Guo-Yi Tang ◽

Pin-He Liu ◽

Chan-Juan Zhao ◽

Qing Liu ◽

...

Keyword(s):

Antioxidant Activity ◽

Liver Injury ◽

Medicinal Herbs ◽

Hepatoprotective Effect

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Hepatoprotective Effect of Pretreatment withThymus vulgarisEssential Oil in Experimental Model of Acetaminophen-Induced Injury

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/954136 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 16

Author(s):

Renata Grespan ◽

Rafael Pazinatto Aguiar ◽

Frederico Nunes Giubilei ◽

Rafael Rocco Fuso ◽

Marcio José Damião ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Essential Oil ◽

Radical Scavenging ◽

Clinical Problem ◽

Hepatoprotective Activity ◽

Hepatoprotective Effect ◽

Thymus Vulgaris ◽

Histopathological Analysis

Acute liver damage caused by acetaminophen overdose is a significant clinical problem and could benefit from new therapeutic strategies.Objective. This study investigated the hepatoprotective effect ofThymus vulgarisessential oil (TEO), which is used popularly for various beneficial effects, such as its antiseptic, carminative, and antimicrobial effects. The hepatoprotective activity of TEO was determined by assessing serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in mice. Their livers were then used to determine myeloperoxidase (MPO) enzyme activity and subjected to histological analysis. In vitro antioxidant activity was evaluated by assessing the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•)-scavenging effects of TEO and TEO-induced lipid peroxidation. TEO reduced the levels of the serum marker enzymes AST, ALT, and ALP and MPO activity. The histopathological analysis indicated that TEO prevented acetaminophen-induced necrosis. The essential oil also exhibited antioxidant activity, reflected by its DPPH radical-scavenging effects and in the lipid peroxidation assay. These results suggest that TEO has hepatoprotective effects on acetaminophen-induced hepatic damage in mice.

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