Genome-wide investigation of ZINC-IRON PERMEASE (ZIP) genes in Areca catechu and potential roles of ZIPs in Fe and Zn uptake and transport

Abstract Background The homeodomain-leucine zipper (HD-Zip) gene family is one of the plant-specific transcription factor families, involved in plant development, growth, and in the response to diverse stresses. However, comprehensive analysis of the HD-Zip genes, especially those involved in response to drought and salinity stresses is lacking in sesame (Sesamum indicum L.), an important oil crop in tropical and subtropical areas. Results In this study, 45 HD-Zip genes were identified in sesame, and denominated as SiHDZ01-SiHDZ45. Members of SiHDZ family were classified into four groups (HD-Zip I-IV) based on the phylogenetic relationship of Arabidopsis HD-Zip proteins, which was further supported by the analysis of their conserved motifs and gene structures. Expression analyses of SiHDZ genes based on transcriptome data showed that the expression patterns of these genes were varied in different tissues. Additionally, we showed that at least 75% of the SiHDZ genes were differentially expressed in responses to drought and salinity treatments, and highlighted the important role of HD-Zip I and II genes in stress responses in sesame. Conclusions This study provides important information for functional characterization of stress-responsive HD-Zip genes and may contribute to the better understanding of the molecular basis of stress tolerance in sesame.

Download Full-text

Genome-wide identification and characterization of HD-ZIP genes in potato

Gene ◽

10.1016/j.gene.2019.02.024 ◽

2019 ◽

Vol 697 ◽

pp. 103-117 ◽

Cited By ~ 11

Author(s):

Wan Li ◽

Jieya Dong ◽

Minxuan Cao ◽

Xianxian Gao ◽

Dongdong Wang ◽

...

Keyword(s):

Genome Wide ◽

Identification And Characterization ◽

Zip Genes

Download Full-text

Genome-wide identification and expression profile of HD-ZIP genes in physic nut and functional analysis of the JcHDZ16 gene in transgenic rice

BMC Plant Biology ◽

10.1186/s12870-019-1920-x ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 2

Author(s):

Yuehui Tang ◽

Jian Wang ◽

Xinxin Bao ◽

Mengyu Liang ◽

Huimin Lou ◽

...

Keyword(s):

Functional Analysis ◽

Transgenic Rice ◽

Expression Profile ◽

Physic Nut ◽

Genome Wide ◽

Zip Genes

Download Full-text

Improving Zinc and Iron Accumulation in Maize Grains Using the Zinc and Iron Transporter ZmZIP5

Plant and Cell Physiology ◽

10.1093/pcp/pcz104 ◽

2019 ◽

Vol 60 (9) ◽

pp. 2077-2085 ◽

Cited By ~ 4

Author(s):

Suzhen Li ◽

Xiaoqing Liu ◽

Xiaojin Zhou ◽

Ye Li ◽

Wenzhu Yang ◽

...

Keyword(s):

Double Mutant ◽

Gus Assay ◽

Divalent Metal Ions ◽

Rnai Line ◽

Zn Uptake ◽

Zip Family ◽

Fe Uptake ◽

Low Levels ◽

Iron Regulated Transporter ◽

Uptake And Transport

Abstract Zinc (Zn) and iron (Fe) are essential micronutrients for plant growth. Thus, it is important to understand the mechanisms of uptake, transport and accumulation of these micronutrients in maize to improve crop nutritional quality. Members of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) family are responsible for the uptake and transport of divalent metal ions in plant. Previously, we showed that ZmZIP5 functionally complemented the Zn uptake double mutant zrt1zrt2, Fe-uptake double mutant fet3fet4 in yeast. In our β-glucuronidase (GUS) assay, the germinated seeds, young sheaths, and stems of ZmZIP5-promoter-GUS transgenic plants were stained. We generated and compared two maize lines for this study: Ubi-ZmZIP5, in which ZmZIP5 was constitutively overexpressed, and ZmZIP5i, a RNAi line. At the seedling stage, high levels of Zn and Fe were found in the roots and shoots of Ubi-ZmZIP5 plants, whereas low levels were found in the ZmZIP5i plants. Zn and Fe contents decreased in the seeds of Ubi-ZmZIP5 plants and remained unchanged in the seeds of ZmZIP5i plants. The seeds of Leg-ZmZIP5 plants, in which ZmZIP5 overexpression is specific to the endosperm, had higher levels of Zn and Fe. Our results imply that ZmZIP5 may play a role in Zn and Fe uptake and root-to-shoot translocation. Endosperm-specific ZmZIP5 overexpression could be useful for Zn and Fe biofortification of cereal grains.

Download Full-text

A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members

Plant Molecular Biology ◽

10.1007/s11103-007-9255-7 ◽

2007 ◽

Vol 66 (1-2) ◽

pp. 87-103 ◽

Cited By ~ 116

Author(s):

Adamantia Agalou ◽

Sigit Purwantomo ◽

Elin Övernäs ◽

Henrik Johannesson ◽

Xiaoyi Zhu ◽

...

Keyword(s):

Family Members ◽

Genome Wide ◽

A Genome ◽

Genome Wide Survey ◽

Zip Genes

Download Full-text

Genome-wide analysis of HD-Zip genes in grape (Vitis vinifera)

Tree Genetics & Genomes ◽

10.1007/s11295-014-0827-9 ◽

2014 ◽

Vol 11 (1) ◽

Cited By ~ 9

Author(s):

Haiyang Jiang ◽

Jing Jin ◽

Huan Liu ◽

Qing Dong ◽

Hanwei Yan ◽

...

Keyword(s):

Vitis Vinifera ◽

Genome Wide Analysis ◽

Genome Wide ◽

Zip Genes

Download Full-text

Use of SEM, X-ray analysis and TEM to localize Fe3+ reduction in roots

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100104029 ◽

1988 ◽

Vol 46 ◽

pp. 392-393 ◽

Cited By ~ 3

Author(s):

William P. Wergin ◽

P. F. Bell ◽

Rufus L. Chaney

Keyword(s):

Electron Microscopy ◽

Root Hairs ◽

Rapid Reduction ◽

X Ray ◽

Physical Evidence ◽

Transmission Electron ◽

Young Root ◽

Insoluble Precipitate ◽

Uptake And Transport ◽

Scanning Electron

In dicotyledons, Fe3+ must be reduced to Fe2+ before uptake and transport of this essential macronutrient can occur. Ambler et al demonstrated that reduction along the root could be observed by the formation of a stain, Prussian blue (PB), Fe4 [Fe(CN)6]3 n H2O (where n = 14-16). This stain, which is an insoluble precipitate, forms at the reduction site when the nutrient solution contains Fe3+ and ferricyanide. In 1972, Chaney et al proposed a model which suggested that the Fe3+ reduction site occurred outside the cell membrane; however, no physical evidence to support the model was presented at that time. A more recent study using the PB stain indicates that rapid reduction of Fe3+ occurs in a region of the root containing young root hairs. Furthermore the most pronounced activity occurs in plants that are deficient in Fe. To more precisely localize the site of Fe3+ reduction, scanning electron microscopy (SEM), x-ray analysis, and transmission electron microscopy (TEM) were utilized to examine the distribution of the PB precipitate that was induced to form in roots.

Download Full-text