scholarly journals Association of Pre-diagnostic Antibody Responses to Escherichia coli and Bacteroides fragilis Toxin Proteins with Colorectal Cancer in a European Cohort

Gut Microbes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 1-14
Author(s):  
Julia Butt ◽  
Mazda Jenab ◽  
Jill Werner ◽  
Veronika Fedirko ◽  
Elisabete Weiderpass ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Escherichia Coli ◽  
Bacteroides Fragilis ◽  
Antibody Responses

scholarly journals 16S rRNA of Mucosal Colon Microbiome and CCL2 Circulating Levels Are Potential Biomarkers in Colorectal Cancer

2021 ◽  
Vol 22 (19) ◽  
pp. 10747
Author(s):  
Carmela Nardelli ◽  
Ilaria Granata ◽  
Marcella Nunziato ◽  
Mario Setaro ◽  
Fortunata Carbone ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Escherichia Coli ◽  
16S Rrna ◽  
Bacteroides Fragilis ◽  
Fusobacterium Nucleatum ◽  
Normal Weight ◽  
Western World ◽  
Intestinal Dysbiosis ◽  
Rrna Sequencing ◽  
Circulating Levels

Colorectal cancer (CRC) is one of the most common malignancies in the Western world and intestinal dysbiosis might contribute to its pathogenesis. The mucosal colon microbiome and C-C motif chemokine 2 (CCL2) were investigated in 20 healthy controls (HC) and 20 CRC patients using 16S rRNA sequencing and immunoluminescent assay, respectively. A total of 10 HC subjects were classified as overweight/obese (OW/OB_HC) and 10 subjects were normal weight (NW_HC); 15 CRC patients were classified as OW/OB_CRC and 5 patients were NW_CRC. Results: Fusobacterium nucleatum and Escherichia coli were more abundant in OW/OB_HC than in NW_HC microbiomes. Globally, Streptococcus intermedius, Gemella haemolysans, Fusobacterium nucleatum, Bacteroides fragilis and Escherichia coli were significantly increased in CRC patient tumor/lesioned tissue (CRC_LT) and CRC patient unlesioned tissue (CRC_ULT) microbiomes compared to HC microbiomes. CCL2 circulating levels were associated with tumor presence and with the abundance of Fusobacterium nucleatum, Bacteroides fragilis and Gemella haemolysans. Our data suggest that mucosal colon dysbiosis might contribute to CRC pathogenesis by inducing inflammation. Notably, Fusobacterium nucleatum, which was more abundant in the OW/OB_HC than in the NW_HC microbiomes, might represent a putative link between obesity and increased CRC risk.

scholarly journals The Four Horsemen in Colon Cancer

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Marco Antonio Hernández-Luna ◽  
Sergio López-Briones ◽  
Rosendo Luria-Pérez
Keyword(s):  
Colorectal Cancer ◽  
Escherichia Coli ◽  
Gastrointestinal Tract ◽  
Salmonella Enterica ◽  
Bacteroides Fragilis ◽  
Fusobacterium Nucleatum ◽  
Cancer Development ◽  
Gastrointestinal Neoplasms ◽  
Intestinal Dysbiosis ◽  
Incidence And Mortality

Worldwide, neoplasms of the gastrointestinal tract have a very high incidence and mortality. Among these, colorectal cancer, which includes colon and rectum malignancies, representing both highest incidence and mortality. While gallbladder cancer, another neoplasm associated to gastrointestinal tract occurs less frequently. Genetic factors, inflammation and nutrition are important risk factors associated with colorectal cancer development. Likewise, pathogenic microorganisms inducing intestinal dysbiosis have become an important scope to determine the role of bacterial infection on tumorigenesis. Interestingly, in human biopsies of different types of gastrointestinal tract cancer, the presence of different bacterial strains, such as Fusobacterium nucleatum, Escherichia coli, Bacteroides fragilis and Salmonella enterica have been detected, and it has been considered as a high-risk factor to cancer development. Therefore, pathogens infection could contribute to neoplastic development through different mechanisms; including intestinal dysbiosis, inflammation, evasion of tumoral immune response and activation of pro-tumoral signaling pathways, such as β catenin. Here, we have reviewed the suggested bacterial molecular mechanisms and their possible role on development and progression of gastrointestinal neoplasms, focusing mainly on colon neoplasms, where the bacteria Fusobacterium nucleatum, Escherichia coli, Bacteroides fragilis and Salmonella enterica infect.

scholarly journals Association between colorectal cancer and Fusobacterium nucleatum and Bacteroides fragilis bacteria in Iranian patients: a preliminary study

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Aref Shariati ◽  
Shabnam Razavi ◽  
Ehsanollah Ghaznavi-Rad ◽  
Behnaz Jahanbin ◽  
Abolfazl Akbari ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Relative Abundance ◽  
Normal Tissue ◽  
Bacteroides Fragilis ◽  
Fusobacterium Nucleatum ◽  
Normal Mucosa ◽  
Clinicopathologic Characteristics ◽  
Tissue Samples ◽  
Adjacent Normal Mucosa ◽  
Iranian Patients

Abstract Background and aim Recent studies have proposed that commensal bacteria might be involved in the development and progression of gastrointestinal disorders such as colorectal cancer (CRC). Therefore, in this study, the relative abundance of Fusobacterium nucleatum, Bacteroides fragilis, Streptococcus bovis/gallolyticus, and Enteropathogenic Escherichia coli (EPEC) in CRC tissues, and their association with clinicopathologic characteristics of CRC was investigated in Iranian patients. Moreover, the role of these bacteria in the CRC-associated mutations including PIK3CA, KRAS, and BRAF was studied. Method To these ends, the noted bacteria were quantified in paired tumors and normal tissue specimens of 30 CRC patients, by TaqMan quantitative Real-Time Polymerase Chain Reaction (qPCR). Next, possible correlations between clinicopathologic factors and mutations in PIK3CA, KRAS, and BRAF genes were analyzed. Results In studied samples, B. fragilis was the most abundant bacteria that was detected in 66 and 60% of paired tumor and normal samples, respectively. Furthermore, 15% of the B. fragilis-positive patients were infected with Enterotoxigenic B. fragilis (ETBF) in both adenocarcinoma and matched adjacent normal samples. F. nucleatum was also identified in 23% of tumors and 13% of adjacent normal tissue samples. Moreover, the relative abundance of these bacteria determined by 2-ΔCT was significantly higher in CRC samples than in adjacent normal mucosa (p < 0.05). On the other hand, our findings indicated that S. gallolyticus and EPEC, compared to adjacent normal mucosa, were not prevalent in CRC tissues. Finally, our results revealed a correlation between F. nucleatum-positive patients and the KRAS mutation (p = 0.02), while analyses did not show any association between bacteria and mutation in PIK3CA and BRAF genes. Conclusion The present study is the first report on the analysis of different bacteria in CRC tissue samples of Iranian patients. Our findings revealed that F. nucleatum and B. fragilis might be linked to CRC. However, any link between gut microbiome dysbiosis and CRC remains unknown.

scholarly journals Mother-to-infant transmission of the carcinogenic colibactin-producing bacteria

2020 ◽  
Author(s):  
Kenji Watanabe ◽  
Yuta Tsunematsu ◽  
Koji Hosomi ◽  
Jun Kunisawa ◽  
Michio Sato ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Escherichia Coli ◽  
Preventive Measures ◽  
Biosynthetic Gene ◽  
Month Of Birth ◽  
Natural Childbirth ◽  
Fecal Samples ◽  
Infection Route

Abstract While colibactin-producing Escherichia coli is linked to colorectal oncogenesis, its infection route remains poorly characterized. Here, analysis of fecal samples of infants over the first month of birth for the presence of a colibactin biosynthetic gene revealed that the bacterium may be transmitted from mother to infant through intimate contacts, such as natural childbirth and breastfeeding. This finding suggests the possibility of developing early preventive measures against colorectal cancer.

scholarly journals Pola kuman dari infeksi luka operasi pada pasien multitrauma

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Sidhit Barung ◽  
Heber B. Sapan ◽  
Winfrid M. Sumanti ◽  
Rudy Tubagus
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Bacteroides Fragilis ◽  
Enterobacter Cloacae ◽  
Alcaligenes Faecalis ◽  
Cross Sectional ◽  
Enterobacter Hormaechei ◽  
Cross Sectional Design ◽  
Pcr Test

Abstract: Surgical site infection (SSI) is the main surgery complication which can increase morbidity, mortality, as well as the hospital cost. The prevalence of SSIs at a health care reflects its sevice quality. This study was aimed to obtain the bacterial profile of SSIs among multitraumatic patients at Prof. Dr. R. D. Kandou Hospital from June through December 2016. This was a descriptive study with a cross sectional design. Pus was obtained from SSIs of laparotomy and ORIF operation wounds, and was further examined with PCR. The results showed that of 16 samples, 3 samples were negative (18.75%) and 13 samples were positive (81.25 %). The PCR test showed that the highest percentage of bacteria was Pseudomonas aeruginosa (6 samples; 46.1%), followed by Escherichia coli (2 samples; 15.4%), and Enterobacter hormaechei, Alcaligenes faecalis, Enterobacter cloacae, Bacteroides fragilis as well as Proteus mirabilis (each of 1 sample; 7.7%). Conclusion: Based on the PCR test, there were 7 types of bacteria at the SSIs of multitraumatic patients at Prof. Dr. R. D. Kandou Hospital Manado, all of them were Gram negative, and the most common type was Pseudomonas aeruginosa.Keywords: bacterial profile, PCR, SSIs, multitraumatic patientsAbstrak: Infeksi luka operasi merupakan salah satu komplikasi utama operasi yang dapat meningkatkan morbiditas, mortalitas, dan biaya perawatan penderita di rumah sakit. Angka kejadian infeksi luka operasi pada suatu institusi penyedia pelayanan kesehatan mencerminkan kualitas pelayanan pada institusi tersebut. Penelitian ini bertujuan untuk mendapatkan pola kuman infeksi luka operasi pada pasien multitrauma di ruang perawatan bedah RSUP Prof. Dr. R. D. Kandou selama bulan Juni-Desember 2016. Jenis penelitian ialah deskriptif dengan desain potong lintang. Apusan pus diambil dari luka operasi terinfeksi pada tindakan laparotomi dan ORIF kemudian diperiksa dengan PCR. Hasil penelitian memperlihatkan dari 16 sampel yang diteliti ditemukan 3 sampel negatif (18,75%) dan 13 sampel positif (81,25 %). Berdasarkan hasil PCR ditemukan pertumbuhan kuman terbanyak ialah Pseudomonas aeruginosa sejumlah 6 sampel (46,1%), diikuti Escherichia coli sejumlah 2 sampel (15,4%), serta Enterobacter hormaechei, Alkaligenes faecalis, Enterobacter cloacae, Bacteroides fragilis, dan Proteus mirabilis, masing-masing sejumlah 1 sampel (7,7%). Simpulan: Berdasarkan hasil PCR didapatkan 7 jenis kuman pada infeksi luka operasi dari pasien multitrauma di RSUP Prof. Dr. R. D. Kandou Manado, kesemuanya tergolong bakteri Gram negatif, dan yang tersering ialah Pseudomonas aeruginosa.Kata kunci: pola kuman, PCR, infeksi luka operasi, pasien multitrauma

Escherichia coli and colorectal cancer: Unfolding the enigmatic relationship

2021 ◽  
Vol 22 ◽  
Author(s):  
Rogayeh Nouri ◽  
Alka Hasani ◽  
Kourosh Masnadi Shirazi ◽  
Mohammad Reza Aliand ◽  
Bita Sepehri ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Escherichia Coli ◽  
Mammalian Cells ◽  
Chromosomal Rearrangements ◽  
Current Evidence ◽  
Dna Breaks ◽  
Colon Cells ◽  
E Coli ◽  
Dna Mismatch ◽  
Double Strand Dna Breaks

: Colorectal cancer (CRC) is one of the deadliest cancers in the world. Specific strains of intestinal Escherichia coli (E. coli) may influence the initiation and development of CRC by exploiting virulence factors and inflammatory pathways. Mucosa-associated E. coli strains are more prevalent in CRC biopsies in comparison to healthy controls. Moreover, these strains can survive and replicate within macrophages and induce a pro-inflammatory response. Chronic exposure to inflammatory mediators can lead to increased cell proliferation and cancer. Production of colobactin toxin by the majority of mucosa-associated E. coli isolated from CRC patients is another notable finding. Colibactin-producing E. coli strains, in particular, induce double-strand DNA breaks, stop the cell cycle, involve in chromosomal rearrangements of mammalian cells and are implicated in carcinogenic effects in animal models. Moreover, some enteropathogenic E. coli (EPEC) strains are able to survive and replicate in colon cells as chronic intracellular pathogens and may promote susceptibility to CRC by downregulation of DNA Mismatch Repair (MMR) proteins. In this review, we discuss current evidence and focus on the mechanisms by which E. coli can influence the development of CRC.

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