Bifacial biological effects of ethanol: acetaldehyde production by oral Streptococcus species and the antibacterial effects of ethanol against these bacteria

The bark of various tree species is a byproduct of the forestry industry that is not used at its full potential, considering the wide range of phytochemicals that are contained in these vegetal matrices and the health benefits that these compounds could provide for society. Our goal was to assess and compare the phytochemical composition of some hydroalcoholic spruce (Picea abies) bark extracts attained by ultrasound assisted extraction (UAE) and microwave-assisted extraction (MAE) and their antioxidant and antibacterial effects. The levels of total phenolics and tannins in the bark extracts were determined using methods based on the Folin–Ciocâlteu reagent, while specific phenolic and volatile compounds were identified and quantified using an UPLC-PDA method and a GC-FID method, respectively. After the chemical composition assessment, the antioxidant capacity (AC) was evaluated by measuring the scavenging ability against two free radicals (DPPH and ABTS). The minimum inhibitory concentration (MIC) was determined to assess the antibacterial activity of the extracts. The results indicated that the extracts produced by UAE had higher contents of polyphenols and tannins and also a higher content of the main phenolic compounds identified, catechin and epicatechin, compared to the MAE extracts. In contrast the highest content of volatile terpenoids (mainly α- and β-pinene) was found in the MAE extracts. All of the tested extracts exhibited relatively high antioxidant activities (especially the UAE extracts) and low MICs against Gram-positive bacteria but were mildly efficient against Gram-negative bacteria. These findings show that the spruce bark might be an important source of bioactive compounds that can be easily extracted from these industrial secondary products. Various uses of this vegetal material may emerge, due to its antioxidant and antibacterial effects.

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Relationship of adhesion in vitro and cariogenicity of oral Streptococcus species in germ-free rats

Archives of Oral Biology ◽

10.1016/0003-9969(85)90085-8 ◽

1985 ◽

Vol 30 (8) ◽

pp. 635-639 ◽

Cited By ~ 5

Author(s):

M.D.P. Willcox ◽

D.B. Drucker ◽

V.F. Hillier ◽

R.M. Green

Keyword(s):

Germ Free ◽

Streptococcus Species ◽

Oral Streptococcus ◽

Relationship Of

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Metabolic property of acetaldehyde production from ethanol and glucose by oral Streptococcus and Neisseria

Scientific Reports ◽

10.1038/s41598-019-46790-9 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 6

Author(s):

Ryo Tagaino ◽

Jumpei Washio ◽

Yuki Abiko ◽

Naoko Tanda ◽

Keiichi Sasaki ◽

...

Keyword(s):

Oral Streptococcus ◽

Acetaldehyde Production

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Utilization of mucin by oral Streptococcus species

Antonie van Leeuwenhoek ◽

10.1007/bf00403951 ◽

1990 ◽

Vol 57 (3) ◽

pp. 165-172 ◽

Cited By ~ 43

Author(s):

J. S. van der Hoeven ◽

C. W. A. van den Kieboom ◽

P. J. M. Camp

Keyword(s):

Streptococcus Species ◽

Oral Streptococcus

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Growth of oral Streptococcus species and Actinomyces viscosus in human saliva.

Applied and Environmental Microbiology ◽

10.1128/aem.47.5.901-904.1984 ◽

1984 ◽

Vol 47 (5) ◽

pp. 901-904 ◽

Cited By ~ 52

Author(s):

M H de Jong ◽

J S van der Hoeven ◽

J H van OS ◽

J H Olijve

Keyword(s):

Human Saliva ◽

Streptococcus Species ◽

Oral Streptococcus

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Optimized Antibacterial Effects in a Designed Mixture of Essential Oils of Myrtus communis, Artemisia herba-alba and Thymus serpyllum for Wide Range of Applications

Foods ◽

10.3390/foods11010132 ◽

2022 ◽

Vol 11 (1) ◽

pp. 132

Author(s):

Wessal Ouedrhiri ◽

Hamza Mechchate ◽

Sandrine Moja ◽

Sylvie Baudino ◽

Asmaa Saleh ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Design Method ◽

Biological Effects ◽

Mixture Design ◽

Chemical Compositions ◽

Myrtus Communis ◽

Antibacterial Effects ◽

Wide Range ◽

Thymus Serpyllum

Nowadays, the combination of molecules influences their biological effects, and interesting outcomes can be obtained from different component interactions. Using a mixture design method, this research seeks to simulate the efficacy of essential oil combinations against various bacteria and forecast the ideal combination. The chemical compositions of Myrtus communis, Artemisia herba-alba and Thymus serpyllum essential oils were analyzed using CG/MS. Then, the combined antibacterial effects were evaluated by testing mixture design formulations using the microdilution bioassay. The main compounds detected for M. communis essential oil were myrtenyl acetate (33.67%), linalool (19.77%) and 1,8-cineole (10.65%). A. herba-alba had piperitone as a chemotype, representing 85%. By contrast, the T. serpyllum oil contained thymol (17.29%), γ-terpinene (18.31%) and p-cymene (36.15%). The antibacterial effect of the essential oils studied, and the optimum mixtures obtained were target strain-dependent. T. serpyllum alone ensured the optimal inhibition against S. aureus and E. coli, while a ternary mixture consisting of 17.1%, 39.6% and 43.1% of M. communis, A. herba-alba and T. serpyllum respectively, was associated with optimal inhibitory activity against B. subtilis. The outcome of this research supports the idea of the boosting effect of essential oil combinations toward better activities, giving better understanding of the usefulness of mixture designs for food, cosmetics, and pharmaceutical applications.

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Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100095017 ◽

1972 ◽

Vol 30 ◽

pp. 350-351

Author(s):

K. Shankar Narayan ◽

Kailash C. Gupta ◽

Tohru Okigaki

Keyword(s):

Ultraviolet Light ◽

Mammalian Cells ◽

Biological Effects ◽

Survival Rates ◽

Chinese Hamster ◽

Cell Types ◽

Differential Sensitivity ◽

Ultrastructural Changes ◽

Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

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Genomic analysis of C-type lectins

Biochemical Society Symposium ◽

10.1042/bss0690059 ◽

2002 ◽

Vol 69 ◽

pp. 59-72 ◽

Cited By ~ 85

Author(s):

Kurt Drickamer ◽

Andrew J. Fadden

Keyword(s):

Biological Effects ◽

Cell Signalling ◽

Genomic Analysis ◽

Binding Activity ◽

Immunoglobulin Superfamily ◽

Carbohydrate Binding ◽

Carbohydrate Recognition ◽

Calcium Dependent ◽

Binding Domains ◽

Carbohydrate Recognition Domains

Many biological effects of complex carbohydrates are mediated by lectins that contain discrete carbohydrate-recognition domains. At least seven structurally distinct families of carbohydrate-recognition domains are found in lectins that are involved in intracellular trafficking, cell adhesion, cell–cell signalling, glycoprotein turnover and innate immunity. Genome-wide analysis of potential carbohydrate-binding domains is now possible. Two classes of intracellular lectins involved in glycoprotein trafficking are present in yeast, model invertebrates and vertebrates, and two other classes are present in vertebrates only. At the cell surface, calcium-dependent (C-type) lectins and galectins are found in model invertebrates and vertebrates, but not in yeast; immunoglobulin superfamily (I-type) lectins are only found in vertebrates. The evolutionary appearance of different classes of sugar-binding protein modules parallels a development towards more complex oligosaccharides that provide increased opportunities for specific recognition phenomena. An overall picture of the lectins present in humans can now be proposed. Based on our knowledge of the structures of several of the C-type carbohydrate-recognition domains, it is possible to suggest ligand-binding activity that may be associated with novel C-type lectin-like domains identified in a systematic screen of the human genome. Further analysis of the sequences of proteins containing these domains can be used as a basis for proposing potential biological functions.

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