scholarly journals STUDIES ON THE PATHOGENESIS OF FEVER

1957 ◽  
Vol 106 (2) ◽  
pp. 293-314 ◽  
Author(s):  
Robert G. Petersdorf ◽  
Ivan L. Bennett
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Essential Role ◽  
Nitrogen Mustard ◽  
Lysergic Acid ◽  
The Other ◽  
Endogenous Pyrogen ◽  
Endogenous Factor ◽  
Bacterial Endotoxins ◽  
Endogenous Serum Pyrogen

Intravenous administration of bacterial endotoxins in dogs is followed within 2 hours by the appearance of a fever-producing substance in the blood. This endogenous pyrogen differs from the endotoxins originally administered by its ability to produce fever in tolerant recipients and failure to promote tolerance after repeated daily injections. Endogenous serum pyrogen is destroyed by heating at 90°C. for 30 minutes, and is also inactivated to some degree by incubation at 37°C. for 24 hours. Suppression of fever by aminopyrine does not affect appearance of the endogenous factor. Animals made febrile with dinitrophenol, kaolin, or lysergic acid do not elaborate a fever-promoting substance in the blood. Sterile abscesses, accompanied by elevations in body temperature of the host, are unassociated with detectable amounts of secondary pyrogen in the serum. The absence of endogenous pyrogen in the blood of febrile dogs made leukopenic with nitrogen mustard favors the idea that polymorphonuclear leukocytes injured by endotoxins release the endogenous factor. On the other hand, the finding that the granulocytopenic animals are febrile when no circulating endogenous pyrogen is present, casts doubt upon the essential role of this substance in endotoxin fever.

The Gastronomy of the Eye

2020 ◽  
pp. 175-198
Author(s):  
Vrushali Dhage
Keyword(s):  
Essential Role ◽  
Historical Perspective ◽  
Smart Cities ◽  
Methodological Approach ◽  
Economic Liberalization ◽  
The Other ◽  
Works Of Art

Works of art can be read at various levels: from being objects of simple retinal pleasure to the other extreme of being significant critical statements of their time. This chapter aims to strike a cerebral dialogue through the works of art. The current study shall consider the latter function of art and analyze the methods in which contemporary Indian artists have made attempts to provide a critique of the early initiatives towards developing Delhi and Mumbai as ‘smart cities'. The review of works from India concludes the essential role of infrastructural projects and envisioned spaces built in the era of economic liberalization. The study aims at drawing a methodological approach, with an art historical perspective, with the artists analysing and translating the urban experiential phenomenon, into artworks.

scholarly journals RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit

2019 ◽  
Vol 47 (17) ◽  
pp. 8975-8987 ◽  
Author(s):  
Paula Allepuz-Fuster ◽  
Michael J O’Brien ◽  
Noelia González-Polo ◽  
Bianca Pereira ◽  
Zuzer Dhoondia ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Essential Role ◽  
Transcription Termination ◽  
Active Role ◽  
The Other ◽  
Loop Formation ◽  
Direct Role

AbstractGene loops are formed by the interaction of initiation and termination factors occupying the distal ends of a gene during transcription. RNAPII is believed to affect gene looping indirectly owing to its essential role in transcription. The results presented here, however, demonstrate a direct role of RNAPII in gene looping through the Rpb4 subunit. 3C analysis revealed that gene looping is abolished in the rpb4Δ mutant. In contrast to the other looping-defective mutants, rpb4Δ cells do not exhibit a transcription termination defect. RPB4 overexpression, however, rescued the transcription termination and gene looping defect of sua7-1, a mutant of TFIIB. Furthermore, RPB4 overexpression rescued the ssu72-2 gene looping defect, while SSU72 overexpression restored the formation of gene loops in rpb4Δ cells. Interestingly, the interaction of TFIIB with Ssu72 is compromised in rpb4Δ cells. These results suggest that the TFIIB–Ssu72 interaction, which is critical for gene loop formation, is facilitated by Rpb4. We propose that Rpb4 is promoting the transfer of RNAPII from the terminator to the promoter for reinitiation of transcription through TFIIB–Ssu72 mediated gene looping.

scholarly journals THE ROLE OF POLYMORPHONUCLEAR LEUKOCYTES IN THE INITIATION AND CESSATION OF THE ARTHUS VASCULITIS

1959 ◽  
Vol 110 (3) ◽  
pp. 481-494 ◽  
Author(s):  
Charles G. Cochrane ◽  
William O. Weigle ◽  
Frank J. Dixon
Keyword(s):  
Inflammatory Reaction ◽  
Polymorphonuclear Leukocytes ◽  
Essential Role ◽  
Temporal Relationship ◽  
Mononuclear Cells ◽  
Small Vessels ◽  
Hypersensitivity Vasculitis ◽  
Inflammatory Site ◽  
Damaged Vessel

The role of polymorphs in the Arthus type hypersensitivity vasculitis has been studied. Polymorphs were found to play an essential role in not only producing the inflammatory vasculitis, but also were instrumental in ridding the damaged vessel of the antigen, probably by means of proteolytic catabolism at the inflammatory site. A temporal relationship between the disappearance of antigen from the damaged vessels and a decrease in inflammatory reaction was found. The earliest localization of antigen and its associated rabbit globulin in the Arthus vasculitis was found to be beneath the endothelium of small vessels. Since submitting this article for publication, Sorkin and Boyden (J. Immunol., 1959, 82, 332) have reported the catabolism of antigen in the presence of antibody by mononuclear cells obtained from the peritoneal cavities of guinea pigs. Evidence was presented indicating that the antigen molecule was actually broken down by the mononuclear cells. Techniques similar to those reported here were used.

scholarly journals The Roles of Sodium and Potassium Ions in the Generation of the Electro-Olfactogram

1968 ◽  
Vol 51 (4) ◽  
pp. 552-578 ◽  
Author(s):  
Sadayuki F. Takagi ◽  
Gordon A. Wyse ◽  
Harunobu Kitamura ◽  
Katsuhiro Ito
Keyword(s):  
Essential Role ◽  
Ringer Solution ◽  
The Other ◽  
Potassium Ions ◽  
Ionic Mechanism ◽  
Ringer’S Solution ◽  
Sodium And Potassium

In order to clarify whether or not the electronegative olfactory mucosal potentials (EOG) are generator potentials, the effects of changed ionic enviroment were studied. The EOG decreased in amplitude and in some cases nearly or completely disappeared, when Na+ in the bathing Ringer solution was replaced by sucrose, Li+, choline+, tetraethylammonium+ (TEA), or hydrazine. In the K+-free Ringer solution, the negative EOG's initially increased and then decreased in amplitude. In Ringer's solution with increased K+, the negative EOG's increased in amplitude. When K+ was increased in exchange for Na+ in Ringer's solution, the negative EOG's decreased, disappeared, and then reversed their polarity (Fig. 6). Next, when the K+ was replaced by equimolar sucrose, Li+, choline+, TEA+, hydrazine, or Na+, the reversed potentials recovered completely only in Na+-Ringer's solution, but never in the other solutions. Thus, the essential role of Na+ and K+ in the negative EOG's was demonstrated. Ba++ was found to depress selectively the electropositive EOG, but it hardly decreased and never increased the negative EOG. Hence, it is concluded that Ba++ interferes only with Cl- influx, and that the negative EOG's are elicited by an increase in permeability of the olfactory receptive membrane to Na+ and K+, but not to Cl-. From the ionic mechanism it is inferred that the negative EOG's are in most cases composites of generator and positive potentials.

scholarly journals DRIS Norms and Sufficiency Ranges for Pepper Grown under Greenhouses Conditions in the Southeast of Spain

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 837
Author(s):  
Alfonso Llanderal ◽  
Pedro García-Caparrós ◽  
María Teresa Lao ◽  
Maria Luz Segura
Keyword(s):  
Capsicum Annuum ◽  
Fruit Development ◽  
Essential Role ◽  
Integrated System ◽  
The Other ◽  
Phenological Stages ◽  
Capsicum Annuum L ◽  
Crop Nutrition

The aim of this study was to compile DRIS (Diagnosis and Recommendation Integrated System) norms and sufficiency ranges for pepper grown in greenhouses and the determination of the variation in DRIS norms and sufficiency ranges during the different phenological stages of the crop. The DRIS norms and sufficiency ranges were constituted by 367 leaf samples of Capsicum annuum L. which were collected in different phenological stages of the crop (flowering (FL), fruiting (FT), fruit development (FD), and harvesting (HV)). Two sufficiency ranges were developed; one including all leaf samples and the other considering the leaf samples by phenological stages ((FL) (44), (FT) (96), (FD) (100), and (HV) (127)). Furthermore, 10 DRIS norms and CV were calculated from N, P, K, Ca, and Mg. Two ratios of them were calculated in FL (N/P and N/K), two in FD (N/P and Ca/N) and one in HV (N/P) showing low CV, meaning, according to DRIS norms, the essential role of these nutrients in the crop nutrition. Moreover, the sufficiency ranges computed by all database were: N (34–56), P (1.9–4.3), K (30–51), Ca (12–33), and Mg (4.0–15) (mg g−1 DW), respectively. The data obtained in this experiment highlighted the importance of the sensitivity (low CV) of nutrient in the phenological stages such as happened with N.

scholarly journals Lockdown e “Chiesa in uscita”. Un caso di studio in Italia: la fraternità “AGBP”

2021 ◽  
Vol 6 (13) ◽  
pp. 131-142
Author(s):  
Daniela Turco
Keyword(s):  
Catholic Church ◽  
Essential Role ◽  
Social Bonds ◽  
The Other ◽  
Focus Attention ◽  
Daily Lives ◽  
On Line ◽  
The Many ◽  
The One

The impetuosity of the Sars-Covid 2 infection has been remarkable, quickly upsetting the habits of individuals in every seat of the planet. We can observe, on the one hand, the individuals, lost and called to redefine every area of their daily lives. On the other, the institutions that, seizing this loss, have been called to play their essential role of creating and strengthening social bonds. The Catholic Church has promoted respect for the norms, without renouncing a “religious offer” which seems to have responded to an effective demand for accompaniment with a lot of possibility to chose on line liturgical celebrations, with a multiplicity of forms and possibilities of access. Among the various options possible, it was decided to focus attention on a specific case: the case of the fraternity “Amici di Gesù Buon Pastore” (AGBP), which has experienced a significant increase in its followers in the days of lockdown. In particular, we will ask ourselves what so many spiritual-followers have been looking for and if (and how) this interest, born during the quarantine period, can transform the new forms of religious belonging into a context where the historical religions seem to be unattractive if compared to the many alternatives of spirituality present on the “market”.

scholarly journals The release of an endogenous pyrogen from guinea pig leukocytes in vitro: a new model for investigating the role of lymphocytes in fevers induced by antigen in hosts with delayed hypersensitivity.

1977 ◽  
Vol 145 (5) ◽  
pp. 1288-1298 ◽  
Author(s):  
P Chao ◽  
L Francis ◽  
E Atkins
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Polymorphonuclear Leukocytes ◽  
Delayed Hypersensitivity ◽  
Endogenous Pyrogen ◽  
Phagocytic Cells ◽  
Relationship Of ◽  
The Relationship

Guinea pig periotoneal exudate (PE) cells incubated overnight in vitro with heat-killed Staphylococci released an endogenous pyrogen (EP) that could be assayed by intravenous injection in rabbits. The febrile responses were linearly related to the dosage of EP over an eightfold range. PE cells derived from guinea pigs with delayed hypersensitivity (DH) to bovine gamma globulin (BGG), also released EP when incubated with antigen in vitro. This reaction was specific and did not occur withe PE cells from normal or complete Freund's adjuvant-sensitized guinea pigs. Studies indicated that monos and/or polymorphonuclear leukocytes rather than lymphocytes were the source of EP. However, when incubated with BGG and sufficient dosages of BGG-sensitized lymphocytes, normal PE cells released EP over a 42 h period. These results suggest that antigen stimulates specifically sensitized lymphocytes to release an agent (perhaps a lymphokine) that activates phagocytic cells to release EP. This model offers unique advantages for investigating in vitro the role of the lymphocyte in antigen-induced fever in DH as well as the relationship of this lymphocyte-induced activity to other known biologic activities mediated by antigen stimulated lymphocytes.

scholarly journals METABOLIC PATTERNS IN THREE TYPES OF PHAGOCYTIZING CELLS

1963 ◽  
Vol 17 (3) ◽  
pp. 487-501 ◽  
Author(s):  
Rachel Oren ◽  
Ann E. Farnham ◽  
Kazuhisa Saito ◽  
Eva Milofsky ◽  
Manfred L. Karnovsky
Keyword(s):  
Alveolar Macrophages ◽  
Inorganic Phosphate ◽  
Polymorphonuclear Leukocytes ◽  
Cell Types ◽  
The Other ◽  
Metabolic Energy ◽  
Free Swimming ◽  
Metabolic Characteristics ◽  
Complex Lipids

Some chemical and metabolic characteristics of polymorphonuclear leukocytes and monocytes from peritoneal exudates of the guinea pig, and of alveolar macrophages from the same animal, have been compared. Changes in the metabolic patterns of these three types of cell have been followed during the act of phagocytosis. The effect of conventional inhibitors of metabolism, and of anaerobiosis on the phagocytic ability of each of the three cell types mentioned has also been determined. From these studies it was found that alveolar macrophages depend to a considerable degree upon oxidative phosphorylation to provide energy for phagocytosis. The other two types of cell depend only on glycolysis as the source of metabolic energy for that function. In some experiments aimed at obtaining information on the possible role of complex lipids in the function of the cell membrane, it was noted that phagocytosis stimulated the incorporation of inorganic phosphate-P32 into the phosphatides of both types of cell from peritoneal exudates—whether these were free-swimming or adherent to a surface. This phenomenon has not yet been detected in the case of alveolar macrophages.

Protein C Is Responsible for the Rapid Inactivation of Factor Va following Blood Clotting In Vitro

1994 ◽  
Vol 72 (01) ◽  
pp. 070-073
Author(s):  
Denise E Jackson ◽  
Christina A Mitchell ◽  
Hatem H Salem
Keyword(s):  
Protein C ◽  
Blood Clotting ◽  
Essential Role ◽  
The Other ◽  
Factor V ◽  
Factor Va ◽  
Rapid Inactivation ◽  
Protein C Activity

SummaryWhen whole blood is allowed to clot in vitro, factor V is rapidly activated to factor Va which is subsequently inactivated. We developed two monoclonal anti-protein C antibodies, one of which inhibits protein C activation and the other inhibits protein C activity. The addition of either antibody to blood before clotting in vitro significantly inhibited the inactivation of factor Va, confirming the essential role of protein C in mediating the rapid inactivation of factor Va.

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