Thromboxane generation by human peripheral blood polymorphonuclear leukocytes.

Human peripheral blood polymorphonuclear leukocytes were stimulated to generate thromboxane B2 in a time- and concentration-dependent fashion upon exposure to serum-treated zymosan particles. Conversion by stimulated PMN of [14C] arachidonic acid to [14C]thromboxane B2 was confirmed by thin-layer radiochromatography, radio-gas chromatography, and mass spectrometry. Generation of thromboxane B2 was independent of platelet contamination and could be inhibited by the cyclooxygenase inhibitor, indomethacin. Cells rendered incapable of ingesting particles by treatment with cytochalasin B generated comparable amounts of thromboxane B2. These results suggest that human peripheral blood polymorphonuclear leukocytes synthesize thromboxanes in response to surface stimulation independently of phagocytosis.

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Analysis of 24-hour steroid excretion in patients with congenital adrenal hyperplasia by capillary gas chromatography, thin-layer chromatography and mass spectrometry

Acta Endocrinologica ◽

10.1530/acta.0.107s119 ◽

1984 ◽

Vol 104 (4_Supplb) ◽

pp. S119

Author(s):

H. VIERHAPPER ◽

P. NOVOTNY ◽

W. WALDHÄUSL ◽

H. FRISCH

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Thin Layer ◽

Congenital Adrenal Hyperplasia ◽

Thin Layer Chromatography ◽

Capillary Gas Chromatography ◽

Adrenal Hyperplasia ◽

Steroid Excretion ◽

Layer Chromatography

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Detection of Antibacterial and Antioxidant Compounds in the Essential Oil of Schizonepeta annua (Pall.) Schischk. Using High-Performance Thin-Layer Chromatography–Direct Bioautography and Gas Chromatography–Quadrupole Time-of-Flight Mass Spectrometry

JPC - Journal of Planar Chromatography - Modern TLC ◽

10.1556/1006.2019.32.5.2 ◽

2019 ◽

Vol 32 (5) ◽

pp. 359-364 ◽

Cited By ~ 2

Author(s):

Zehui Li ◽

Xi Bai ◽

Qingling Ma ◽

Haji Akber Aisa ◽

Maitinuer Maiwulanjiang

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Essential Oil ◽

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Antioxidant Compounds ◽

Flight Mass Spectrometry ◽

Direct Bioautography ◽

Layer Chromatography

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Airway synthesis of 20-hydroxyeicosatetraenoic acid: metabolism by cyclooxygenase to a bronchodilator

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1999.276.2.l280 ◽

1999 ◽

Vol 276 (2) ◽

pp. L280-L288 ◽

Cited By ~ 15

Author(s):

Elizabeth R. Jacobs ◽

Richard M. Effros ◽

John R. Falck ◽

K. Malla Reddy ◽

William B. Campbell ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Arachidonic Acid ◽

Airway Resistance ◽

Acid Metabolism ◽

Gas Chromatography Mass Spectrometry ◽

Dependent Manner ◽

Hydroxyeicosatetraenoic Acid ◽

Polar Metabolites ◽

H 20

Rabbit airway tissue is a particularly rich source of cytochrome P-4504A protein, but very little information regarding the effect(s) of 20-hydroxyeicosatetraenoic acid (20-HETE) on bronchial tone is available. Our studies examined the response of rabbit bronchial rings to 20-HETE and the metabolism of arachidonic acid and 20-HETE from airway microsomes. 20-HETE (10−8 to 10−6 M) produced a concentration-dependent relaxation of bronchial rings precontracted with KCl or histamine but not with carbachol. Relaxation to 20-HETE was blocked by indomethacin or epithelium removal, consistent with the conversion of 20-HETE to a bronchial relaxant by epithelial cyclooxygenase. A cyclooxygenase product of 20-HETE also elicited relaxation of bronchial rings. [14C]arachidonic acid was converted by airway microsomes to products that comigrated with authentic 20-HETE (confirmed by gas chromatography-mass spectrometry as 19- and 20-HETE) and to unidentified polar metabolites. [3H]20-HETE was metabolized to indomethacin-inhibitable products. These data suggest that 20-HETE is an endogenous product of rabbit airway tissue and may modulate airway resistance in a cyclooxygenase-dependent manner.

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