The murine lymphocyte receptor for IgE. II. Characterization of the multivalent nature of the B lymphocyte receptor for IgE.

The murine B lymphocyte Fc epsilon R is functionally multivalent. Radiolabeled rat IgE, when bound to the B cell Fc epsilon R will co-isolate with the Fc epsilon R on a rat IgE affinity column; examination of the affinity column eluate by SDS-PAGE reveals the component previously identified as the Fc epsilon R as well as E and L chains from IgE. At low levels of Fc epsilon R saturation, up to 30% of the Fc epsilon R bound IgE becomes bound to IgE-Affi-Gel. By using a biotin-avidin system, the coprecipitation of non-haptenated IgE with haptenated IgE was examined and the results suggest (but do not prove) a divalent receptor.

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Interleukin 7-dependent B lymphocyte precursor cells are ultrasensitive to apoptosis.

Journal of Experimental Medicine ◽

10.1084/jem.179.6.1789 ◽

1994 ◽

Vol 179 (6) ◽

pp. 1789-1797 ◽

Cited By ~ 42

Author(s):

S D Griffiths ◽

D T Goodhead ◽

S J Marsden ◽

E G Wright ◽

S Krajewski ◽

...

Keyword(s):

B Cells ◽

B Cell ◽

B Lymphocyte ◽

Precursor Cells ◽

Alpha Particles ◽

Differential Sensitivity ◽

X Rays ◽

Interleukin 7 ◽

Low Levels ◽

B Cell Precursors

We have compared the sensitivity of clonogenic interleukin 7 (IL-7)-dependent murine B cell precursors with that of clonogenic mature B cells and myeloid precursors to alpha-particles from plutonium-238 and X radiation. All three populations are relatively sensitive, but B cell precursors are ultrasensitive. This differential sensitivity is also observed with corticosteroid, etoposide, and cisplatin, all apoptosis-inducing drugs used in the treatment of leukemia and other cancers. Further, we show that x-rays and drugs induce the bulk of the B cell precursor population to undergo rapid apoptosis, despite the continued presence of IL-7. B cell precursors were found to express very low levels of BCL-2 protein compared with mature splenic B cells and their resistance to x-rays and corticosteroid could be enhanced by expression of a BCL-2 transgene. These data have important implications for normal lymphopoiesis and for the behavior of leukemic lymphoid precursor cells.

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Isolation and partial characterization of a lectin from Bauhinia pentandra (bong) vog. Ex. Steua.

Revista Brasileira de Fisiologia Vegetal ◽

10.1590/s0103-31312001000300002 ◽

2001 ◽

Vol 13 (3) ◽

pp. 262-269 ◽

Cited By ~ 7

Author(s):

ANDRÉ LUIS COELHO DA SILVA ◽

ANA CECÍLIA GÓES HORTA ◽

RENATO DE AZEVEDO MOREIRA

Keyword(s):

Protein Band ◽

Carbohydrate Specificity ◽

Original Activity ◽

Divalent Metal Cations ◽

Ph Values ◽

Seed Flour ◽

Sds Page ◽

Low Levels ◽

Sepharose 4B

Bauhinia pentandra (Bong) Vog. ex. Steua seeds were investigated with respect to phenologic aspects (size, mass, hilum and length) and with respect to their chemical composition. The total nitrogen content of the seed flour was determined, and the flour was extracted in different pH values. A lectin was isolated from the seeds by Sepharose-4B affinity chromatography. The homogeneity of the lectin was demonstrated by SDS-PAGE in the presence of beta-mercaptoethanol. Only one protein band with an apparent molecular mass of 30 kDa was found. The B. pentandra lectin showed a carbohydrate specificity for D-galactose, a requirement for divalent metal cations (Ca2+ and Mn2+) for full activity and amino acid composition with a high content of aspartic acid, glutamic acid and alanine and low levels of methionine, cysteine and tryptophan. The lectin agglutinated rabbit and human A group erythrocytes and was relatively stable to heat treatment, retaining half of its original activity after 60 min at 70 ºC.

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Purification and characterization of a 47 kDa protease from Schistosoma mansoni cercarial secretion

Parasitology ◽

10.1017/s0031182000074138 ◽

1992 ◽

Vol 105 (2) ◽

pp. 211-218 ◽

Cited By ~ 13

Author(s):

C. Chavez-Olortegui ◽

M. Resende ◽

C. A. P. Tavares

Keyword(s):

Monoclonal Antibody ◽

Schistosoma Mansoni ◽

Protease Inhibitors ◽

Collagen Type ◽

Affinity Column ◽

Purification And Characterization ◽

Sds Page ◽

Collagen Type Vi ◽

Optimal Ph

SUMMARYFractionation of Schistosoma mansoni cercariae gland secretion on a Sephadex G-150 column followed by a Superose-12 column in an FPLC system, isolated a 47 kDa protease which migrated as a single band on SDS–PAGE gels. A monoclonal antibody (MAb) was produced which recognizes only the 47 kDa protease, and an immuno-affinity column with the MAb was used to isolate the protease. The 47 kDa protease showed activity on several macromolecules such as elastin and collagen type VI besides gelatin and casein. This suggests that this enzyme can be one of the enzymes that might facilitate invasion of the cercariae through host skin. The optimal pH of the protease against the synthetic substrate, Ac-Phe-Arg-Nan, in Tris–HCI buffer was 10. Experiments with protease inhibitors indicate that the purified enzyme is a serine protease.

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Faculty Opinions recommendation of Multiple signaling pathways promote B lymphocyte stimulator dependent B-cell growth and survival.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1101071.557050 ◽

2008 ◽

Author(s):

E Charles Snow

Keyword(s):

Cell Growth ◽

B Cell ◽

Signaling Pathways ◽

B Lymphocyte ◽

Growth And Survival ◽

B Lymphocyte Stimulator ◽

Multiple Signaling ◽

Cell Growth And Survival

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Faculty Opinions recommendation of Characterization of the B cell epitopes associated with a truncated form of Pseudomonas exotoxin (PE38) used to make immunotoxins for the treatment of cancer patients.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1113121.569111 ◽

2008 ◽

Author(s):

Arthur Frankel

Keyword(s):

B Cell ◽

Cancer Patients ◽

Truncated Form ◽

Pseudomonas Exotoxin ◽

B Cell Epitopes

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Faculty Opinions recommendation of Characterization of pediatric Philadelphia-negative B-cell precursor acute lymphoblastic leukemia with kinase fusions in Japan.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726364889.793551058 ◽

2018 ◽

Author(s):

Jan Starý

Keyword(s):

Acute Lymphoblastic Leukemia ◽

B Cell ◽

Lymphoblastic Leukemia ◽

Cell Precursor

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Characterization of Venoms of Deinagkistrodon acutus and Bungarus multicinctus Using Proteomics and Peptidomics

Current Proteomics ◽

10.2174/1570164617666191121112319 ◽

2020 ◽

Vol 17 (3) ◽

pp. 241-254

Author(s):

Yaqiong Zhang ◽

Zhiping Jia ◽

Yunyang Liu ◽

Xinwen Zhou ◽

Yi Kong

Keyword(s):

Snake Venom ◽

Protein Families ◽

Traditional Medicines ◽

Phospholipases A2 ◽

Inhibitory Peptides ◽

Sds Page ◽

Deinagkistrodon Acutus ◽

Venom Proteins ◽

Proteins And Peptides

Background: Deinagkistrodon acutus (D. acutus) and Bungarus multicinctus (B. multicinctus) as traditional medicines have been used for hundreds of years in China. The venoms of these two species have strong toxicity on the victims. Objective: The objective of this study is to reveal the profile of venom proteins and peptides of D. acutus and B. multicinctus. Method: Ultrafiltration, SDS-PAGE coupled with in-gel tryptic digestion and Liquid Chromatography- Electrospray Ionization-Tandem Mass Spectrometry (LC-ESI-MS/MS) were used to characterize proteins and peptides of venoms of D. acutus and B. multicinctus. Results: In the D. acutus venom, 67 proteins (16 protein families) were identified, and snake venom metalloproteinases (SVMPs, 38.0%) and snake venom C-type lectins (snaclecs, 36.7%) were dominated proteins. In the B. multicinctus venom, 47 proteins (15 protein families) were identified, and three-finger toxins (3FTxs, 36.3%) and Kunitz-type Serine Protease Inhibitors (KSPIs, 32.8%) were major components. In addition, both venoms contained small amounts of other proteins, such as Snake Venom Serine Proteinases (SVSPs), Phospholipases A2 (PLA2s), Cysteine-Rich Secreted Proteins (CRISPs), 5'nucleotidases (5'NUCs), Phospholipases B (PLBs), Phosphodiesterases (PDEs), Phospholipase A2 Inhibitors (PLIs), Dipeptidyl Peptidases IV (DPP IVs), L-amino Acid Oxidases (LAAOs) and Angiotensin-Converting Enzymes (ACEs). Each venom also had its unique proteins, Nerve Growth Factors (NGFs) and Hyaluronidases (HYs) in D. acutus, and Cobra Venom Factors (CVFs) in B. multicinctus. In the peptidomics, 1543 and 250 peptides were identified in the venoms of D. acutus and B. multicinctus, respectively. Some peptides showed high similarity with neuropeptides, ACE inhibitory peptides, Bradykinin- Potentiating Peptides (BPPs), LAAOs and movement related peptides. Conclusion: Characterization of venom proteins and peptides of D. acutus and B. multicinctus will be helpful for the treatment of envenomation and drug discovery.

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Characterization of Glycoprotein Fraction from Carp Pituitaries Isolated Using Concanavalin A as the Affinity Ligand

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19980434 ◽

1998 ◽

Vol 63 (3) ◽

pp. 434-440 ◽

Cited By ~ 2

Author(s):

Irena Hulová ◽

Jana Barthová ◽

Helena Ryšlavá ◽

Václav Kašička

Keyword(s):

Concanavalin A ◽

Reversed Phase ◽

Amino Acid Sequences ◽

Gel Chromatography ◽

Affinity Ligand ◽

Sds Page ◽

Terminal Amino ◽

Α And Β Subunits

Glycoproteins that have affinity to Concanavalin A were isolated from the acetone-dried pituitaries of common carp (Cyprinus carpio L.). Two fractions of glycoproteins were separated using gel chromatography on Superdex 75HR. The fraction with lower molecular weight (30 000) corresponding to the carp gonadotropin cGtH II was composed of two subunits as determined using SDS-PAGE. This protein fraction was further divided into four components using reversed-phase HPLC. Two fractions were pure α and β subunits of cGtH II as follows from immunodetection and from determination of N-terminal amino acid sequences. The other two were a mixture of α and β subunits as was also revealed by N-terminal analysis. Capillary electrophoresis was also used for characterization of isolated glycoproteins.

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