scholarly journals DIFFERING RATES OF DEATH AT INNER AND OUTER SURFACES OF THE PROTOPLASM

1944 ◽  
Vol 28 (1) ◽  
pp. 37-42
Author(s):  
W. J. V. Osterhout
Keyword(s):  
Outer Surface ◽  
Coming Out ◽  
Negative Potential ◽  
Protoplasmic Surface ◽  
Inner Surface

A previous paper showed that when the inner protoplasmic surface has lost its potential under the influence of formaldehyde the outer surface can still respond to changes in the concentration of electrolytes. The present paper indicates that after the inner surface has lost its potential there may be a sudden development of negative potential at the outer surface due to substances coming out of the sap and combining with formaldehyde.

A SEM and Light Microscope Study of the Epidermal Leaf Structures of the Carnivorous Plant, Sarracenia purpurea, L.

1971 ◽  
Vol 29 ◽  
pp. 358-359
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Outer Surface ◽  
Microscope Study ◽  
Light Microscope ◽  
Carnivorous Plant ◽  
Sarracenia Purpurea ◽  
Inner Surface

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

Ultrastructure and development of urediospore ornamentation in Melampsora lini

1971 ◽  
Vol 49 (12) ◽  
pp. 2067-2073 ◽  
Author(s):  
L. J. Littlefield ◽  
C. E. Bracker
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Outer Surface ◽  
Spore Wall ◽  
Wall Material ◽  
Wild Type ◽  
Melampsora Lini ◽  
Inner Surface ◽  
External Position ◽  
Basal Portion

The urediospores of Melampsora lini (Ehrenb.) Lev. are echinulate, with spines ca. 1 μ long over their surface. The spines are electron-transparent, conical projections, with their basal portion embedded in the electron-dense spore wall. The entire spore, including the spines, is covered by a wrinkled pellicle ca. 150–200 Å thick. The spore wall consists of three recognizable layers in addition to the pellicle. Spines form initially as small deposits at the inner surface of the spore wall adjacent to the plasma membrane. Endoplasmic reticulum occurs close to the plasma membrane in localized areas near the base of spines. During development, the spore wall thickens, and the spines increase in size. Centripetal growth of the wall encases the spines in the wall material. The spines progressively assume a more external position in the spore wall and finally reside at the outer surface of the wall. A mutant strain with finely verrucose spores was compared to the wild type. The warts on the surface of the mutant spores are rounded, electron-dense structures ca. 0.2–0.4 μ high, in contrast to spines of the wild type. Their initiation near the inner surface of the spore wall and their eventual placement on the outer surface of the spore are similar to that of spines. The wall is thinner in mutant spores than in wild-type spores.

Development of Inverse Analysis of Heat Conduction and Thermal Stress for Elbow: Part I

2013 ◽  
Author(s):  
Seiji Ioka ◽  
Shiro Kubo ◽  
Mayumi Ochi ◽  
Kiminobu Hojo
Keyword(s):  
Heat Conduction ◽  
Thermal Stress ◽  
Transfer Function ◽  
Surface Temperature ◽  
Outer Surface ◽  
Inverse Analysis ◽  
Temperature History ◽  
Analysis Method ◽  
Inner Surface ◽  
Inverse Analysis Method

Thermal fatigue may develop in piping elbow with high temperature stratified flow. To prevent the fatigue damage by stratified flow, it is important to know the distribution of thermal stress and temperature history in a pipe. In this study, heat conduction inverse analysis method for piping elbow was developed to estimate the temperature history and thermal stress distribution on the inner surface from the outer surface temperature history. In the inverse analysis method, the inner surface temperature was estimated by using the transfer function database which interrelates the inner surface temperature with the outer surface temperature. Transfer function database was calculated by FE analysis in advance. For some patterns of the temperature history, inverse analysis simulations were made. It was found that the inner surface temperature history was estimated with high accuracy.

Expansion of the area vasculosa of the chick after removal of the ectoderm

Development ◽  
1970 ◽  
Vol 24 (1) ◽  
pp. 95-108
Author(s):  
J. M. Augustine
Keyword(s):  
Basement Membrane ◽  
Outer Surface ◽  
Chicken Embryo ◽  
Area Vasculosa ◽  
Inner Surface

The role of the ectoderm in the expansion of the mesoderm in the area vasculosa of the chicken embryo was studied. The basement membrane of the ectoderm was found to constitute a substratum for the expansion of both layers of mesoderm, since (a) the somatic mesoderm, particularly at its margin, adheres to the basement membrane, and (b) the somatic and splanchnic mesoderm adhere to each other throughout most of the area opaca. Following removal of the ectoderm from the outer surface of the basement membrane, movement of the underlying mesoderm along its inner surface stopped. Mean expansion of the mesoderm in these cases was zero. Following removal of both ectoderm and basement membrane, expansion of the underlying mesoderm was normal in amount. Experimental changes in the ectodermal substratum can thus stop movement of the associated mesoderm, but the role which the substratum normally plays in mesodermal expansion remains unclear.

Parametric Study of Dry Sliding Wear Behaviour of Functionally Graded Al LM25/Si3N4 Composite by Response Surface Methodology

2015 ◽  
Vol 24 (6) ◽  
pp. 096369351502400 ◽  
Author(s):  
N. Radhika ◽  
R. Raghu
Keyword(s):  
Response Surface Methodology ◽  
Wear Rate ◽  
Response Surface ◽  
Outer Surface ◽  
Sliding Wear ◽  
Centrifugal Casting ◽  
Functionally Graded ◽  
Wear Behaviour ◽  
Inner Surface ◽  
Sliding Distance

Functionally graded aluminium LM25/silicon nitride composite was produced through stir casting followed by centrifugal casting and obtained a hollow cylindrical cast component with dimensions of 150 × 150 × 20 mm. The microstructural examination and the hardness test were carried out on the outer (1 mm) and inner surface (17 mm) as the function of radial distance from the outer periphery. The outer surface was observed with particle enriched region compared to inner surface and exhibited higher hardness. Hence the outer surface of the functionally graded composite was only further subjected to sliding wear test in pin-on-disc tribometer. The Central Composite Design in Response Surface Methodology was used to design the experiments for the selected parameters such as load (15–45 N), velocity (0.5–2.5 m/s) and sliding distance (500–2000 m). Regression test and Analysis of Variance were conducted to check the adequacy of the constructed model. The surface plots for wear rate showed that wear rate increased with increase in load and non-linearly varied with increase in velocity and sliding distance. Scanning Electron Microscopy analysis was conducted on the worn-out surfaces and observed mild to severe wear transition on increase of load.

scholarly journals ACTION CURVES WITH SINGLE PEAKS IN NITELLA IN RELATION TO THE MOVEMENT OF POTASSIUM

1940 ◽  
Vol 23 (6) ◽  
pp. 743-748 ◽  
Author(s):  
W. J. V. Osterhout ◽  
S. E. Hill
Keyword(s):  
Outer Surface ◽  
Distilled Water ◽  
Protoplasmic Surface ◽  
Outer Protoplasmic Surface ◽  
Two Peaks

In Nitella the action curve has two peaks, apparently because both protoplasmic surfaces (inner and outer) are sensitive to K+. Leaching in distilled water makes the outer surface insensitive to K+. We may therefore expect the action curve to have only one peak. This expectation is realized. The action curve thus obtained resembles that of Chara which has an outer protoplasmic surface that is normally insensitive to K+. The facts indicate that the movement of K+ plays an important part in determining the shape of the action curve.

Controlled Synthesis of PbWO4 Crystals with Good Fluorescence Property by a Novel Duck Egg Membrane

2015 ◽  
Vol 33 ◽  
pp. 49-59
Author(s):  
Bao You Gong ◽  
Le Pan ◽  
Qing Feng Zhang ◽  
Hui Zhang ◽  
An Jian Xie ◽  
...  
Keyword(s):  
Outer Surface ◽  
Fluorescence Spectra ◽  
Room Temperature ◽  
Blue Shift ◽  
Biomimetic Synthesis ◽  
Reactant Concentration ◽  
Inner Surface ◽  
Egg Membrane ◽  
Duck Egg ◽  
Slight Blue Shift

PbWO4 crystals with different morphologies were readily induced by duck egg membrane via biomimetic synthesis at room temperature. The size and morphologies of the PbWO4 crystals could be controlled by outer or inner surface of duck egg membrane, the reactant concentration and the reaction time. The results show that spherical, flower-like and spindle-like crystals were obtained on the inner surface of membrane while rhombic, hexagon-like and chrysanthemum-like crystals were gained on the outer surface with the concentration of Pb2+ and WO42- increases. Room-temperature fluorescence spectra indicate the products on the inner surface of the duck egg membrane have a slight blue shift compared to that on the outer surface at the same condition. The PbWO4 crystals with small size obtained at a lower reactant concentration present a better fluorescence performance. The exploration of the reaction mechanism reveals that the interaction between Pb2+ ions and the proteins on the surface of duck egg membrane can make the conformation of the proteins more ordered. In general, the present synthesis route may be extended to prepare other inorganic functional micro-materials.

scholarly journals Thermal Elastic-Plastic Stress Analysis of an Aluminium Composite Disc under Linearly Decreasing Thermal Loading

2008 ◽  
Vol 17 (3) ◽  
pp. 096369350801700 ◽  
Author(s):  
Muzaffer Topcu ◽  
Gurkan Altan ◽  
Hasan Callioglu ◽  
Burcin Deda Altan
Keyword(s):  
Thermal Stress ◽  
Stress Analysis ◽  
Tangential Stress ◽  
Outer Surface ◽  
Thermal Stresses ◽  
Stress Component ◽  
Aluminium Composite ◽  
Hardening Material ◽  
Elastic Plastic ◽  
Inner Surface

In this study, an elastic-plastic thermal stress analysis of an orthotropic aluminium metal matrix composite disc with a hole has been investigated analytically for non-linear hardening material behaviour. The aluminium composite disc reinforced curvilinearly by steel fibres is produced under hydraulic press. The mechanical properties of the composite disc are obtained by tests. A computer program is developed to calculate the thermal stresses under a linearly decreasing temperature from inner surface to outer surface. Elastic, elastic-plastic and residual thermal stress distributions are obtained analytically from inner surface to outer surface and they are presented in tables and Fig. s. The elastic-plastic solution is performed for the plastic region expanding around the inner surface. The magnitude of the tangential stress component has been found out in this study to be higher than the magnitude of the radial stress component. Besides, the tangential stress component is compressive at the inner surface and tensile at the outer surface. The magnitude of the tangential residual stress component is the highest at the inner surface of the composite disc.

scholarly journals DIFFERING RATES OF DEATH AT INNER AND OUTER SURFACES OF THE PROTOPLASM

1945 ◽  
Vol 28 (4) ◽  
pp. 343-347 ◽  
Author(s):  
W. J. V. Osterhout
Keyword(s):  
Surface Layer ◽  
Outer Surface ◽  
Diffusion Potential ◽  
Experimental Conditions ◽  
Toxic Agent ◽  
Inner Surface

The inner and outer protoplasmic surfaces in Nitella may differ greatly in behavior. When 0.01 M HgCl2 is applied externally death arrives first at the inner surface. But when 0.01 M HgCl2 + 0.01 M KCl is applied death takes place sooner at the outer surface. Since 0.01 M KCl by itself is not toxic its effect may be to condition the surface layer chemically or by means of the diffusion potential it sets up (this may amount to 100 mv.). These surfaces consist of non-aqueous films forming the boundaries of a layer of aqueous protoplasm not over 10 microns in thickness. These and earlier experiments with formaldehyde and with chloroform show clearly that it is possible to control the behavior of the protoplasmic surfaces so that when a toxic agent is applied it may produce death more rapidly at the inner or at the outer surface according to experimental conditions.

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