Metallic copper nanoparticles induce apoptosis in a human skin melanoma A-375 cell line

MDR is the main obstacle to chemotherapy efficiency. MDR can grow in cancer cells even if only the one cytostatic agent will act. The aim of the nowadays work is to characterize MDR in metastatic human skin melanoma cell lines prepared in “N.N. Blokhin Russian Cancer Research Center”. pgpl70 expression was detected by immunofluorescence methods. mRNA of MDR gene was identified by Reverse Transcriptase- PCR( RT-PCR) method. Rhodamine 123 (Rhl23) emission has been evaluated by flow cyto- fluorimetiy, cytotoxic activity was estimated by MTT-tests. The cells sensitivity to Aianoza cytostatic effects has showed that mel Kor cells were sensitive to Aranoza acting, but mel Ibr and mel Mtp X were not. Mel Ibr cells had expressed pgpl70 from 35 to 50 per cent, it was detected by immunofluorescence reaction. Mel Kor and mel Mtp-X cells were not expressed P-glycoprotein. mRNA of genes responsible for multi-drug resistance - MDR1, BCRP, MRP1 and LRP (MVP) - were detected by PCR. mRNA of BCRP and MRP1 genes has low expression, barely visible stripes after 33 cycles in all cell lines samples. LRP (MVP) genes expression of mRNA, unfortunately, never managed to see. YB1 gene mRNA expression is well, it is typically for cancer cells. mRNA of gene was found in mel MtpX and mel Ibr subclones cell lines. Mel Kor cells didn't contain mRNA of MDR1 gene. The study of the Rhl23 emission from cells showed that mel Kor control cells had accumulated Rhl23 and didn't throw it out. Mel Ibr cell line accumulated Rhl23 and threw out the half part of it. Mel MtpX cell tine had accumulated the less part of Rhl23 and almost all were thrown out. Thus, the study shows that mel Kor cell tine that are sensitive to Aranoza doesn't express pgpl70, not contain mRNA of multi-chug resistance genes and does not throw Rhl23. Mel Ibr cells resistant to the Aranoza cytotoxic action express pgpl70 ,contain mRNA of MDR1 gene and throw out Rhl23. However, mel MtpX cell line resistant to Aranoza does not express pgpl70, but contains mRNA of MDR1 gene and actively throws out Rhl23.

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Isolation and in vitro cytotoxic activity of 11-methylixoside isolated from bark of Randia dumetorum Lamk

Herba Polonica ◽

10.2478/hepo-2013-0005 ◽

2013 ◽

Vol 59 (1) ◽

pp. 44-52 ◽

Cited By ~ 2

Author(s):

Jeet Singh Jangwan ◽

Rita Patrizia Aquino ◽

Teresa Mencherini ◽

Raghubir Singh

Keyword(s):

Cytotoxic Activity ◽

Cell Line ◽

Human Skin ◽

Melanoma Cell ◽

Melanoma Cell Line ◽

Neuroblastoma Cell ◽

Skin Melanoma ◽

Iridoid Glucoside ◽

Medicinal Tree

Summary Randia dumetorum (family Rubiaceae) is highly reputed ayurvedic medicinal tree commonly known as the Mainphal. A large deciduous thorny shrub grows up to 5 m of height. It occurs almost throughout India up to 1200 m of altitude. It is found in Himalaya from Jammu East ward ascending to 400 m and from Kashmir to East ward up to 1200 m. 11-methylixoside (compound 1), an iridoid glucoside, was isolated from the bark of this plant. The structure was characterized by using spectroscopic methods including 1D-1HNMR,13C-NMR and 2D-NMR (HSQC,HMBC, DQF-COSY) experiments and confirmed by comparison of their NMR data with those from the literature. This compound has been reported for the first time in Randia dumetorum bark. The 11-methylixoside was subjected to cytotoxic activity against MDA-MB-231 (breast cancer cell line) and SK-MEL-2 (human skin melanoma cell line), BE(2)C (neuroblastoma cell line derived from human bone marrow) and U87MG (human neuronale glioblastoma (astrozytom) cell line showed appreciable cytotoxic effect with IC50 value 63.10 µg/ml concentration for SK-MEL-2 (human skin melanoma cell line).

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New Hybrid Copper Nanoparticles/Conjugated Polyelectrolyte Composite with Antibacterial Activity

Polymers ◽

10.3390/polym13030401 ◽

2021 ◽

Vol 13 (3) ◽

pp. 401

Author(s):

Ignacio A. Jessop ◽

Yasmín P. Pérez ◽

Andrea Jachura ◽

Hipólito Nuñez ◽

Cesar Saldías ◽

...

Keyword(s):

Antibacterial Activity ◽

Copper Nanoparticles ◽

Salmonella Enteritidis ◽

Theoretical Calculations ◽

Chemical Reduction ◽

Metallic Copper ◽

Resistant Bacteria ◽

Small Contribution ◽

Antibiotic Resistant ◽

Conjugated Polyelectrolyte

In the search for new materials to fight against antibiotic-resistant bacteria, a hybrid composite from metallic copper nanoparticles (CuNPs) and a novel cationic π-conjugated polyelectrolyte (CPE) were designed, synthesized, and characterized. The CuNPs were prepared by chemical reduction in the presence of CPE, which acts as a stabilizing agent. Spectroscopic analysis and electron microscopy showed the distinctive band of the metallic CuNP surface plasmon and their random distribution on the CPE laminar surface, respectively. Theoretical calculations on CuNP/CPE deposits suggest that the interaction between both materials occurs through polyelectrolyte side chains, with a small contribution of its backbone electron density. The CuNP/CPE composite showed antibacterial activity against Gram-positive (Staphylococcus aureus and Enterococcus faecalis) and Gram-negative (Escherichia coli and Salmonella enteritidis) bacteria, mainly attributed to the CuNPs’ effect and, to a lesser extent, to the cationic CPE.

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Silica-coating of metallic copper nanoparticles in aqueous solution

Colloids and Surfaces A Physicochemical and Engineering Aspects ◽

10.1016/j.colsurfa.2007.11.009 ◽

2008 ◽

Vol 317 (1-3) ◽

pp. 756-759 ◽

Cited By ~ 60

Author(s):

Y. Kobayashi ◽

T. Sakuraba

Keyword(s):

Aqueous Solution ◽

Copper Nanoparticles ◽

Metallic Copper ◽

Silica Coating

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Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.

The Journal of Cell Biology ◽

10.1083/jcb.106.3.761 ◽

1988 ◽

Vol 106 (3) ◽

pp. 761-771 ◽

Cited By ~ 2754

Author(s):

P Boukamp ◽

R T Petrussevska ◽

D Breitkreutz ◽

J Hornung ◽

A Markham ◽

...

Keyword(s):

Epithelial Cell ◽

Cell Line ◽

Human Skin ◽

Cell Lines ◽

Hacat Cell ◽

Epithelial Cell Line ◽

Spontaneous Transformation ◽

Chromosomal Alterations ◽

Hacat Cell Line

In contrast to mouse epidermal cells, human skin keratinocytes are rather resistant to transformation in vitro. Immortalization has been achieved by SV40 but has resulted in cell lines with altered differentiation. We have established a spontaneously transformed human epithelial cell line from adult skin, which maintains full epidermal differentiation capacity. This HaCaT cell line is obviously immortal (greater than 140 passages), has a transformed phenotype in vitro (clonogenic on plastic and in agar) but remains nontumorigenic. Despite the altered and unlimited growth potential, HaCaT cells, similar to normal keratinocytes, reform an orderly structured and differentiated epidermal tissue when transplanted onto nude mice. Differentiation-specific keratins (Nos. 1 and 10) and other markers (involucrin and filaggrin) are expressed and regularly located. Thus, HaCaT is the first permanent epithelial cell line from adult human skin that exhibits normal differentiation and provides a promising tool for studying regulation of keratinization in human cells. On karyotyping this line is aneuploid (initially hypodiploid) with unique stable marker chromosomes indicating monoclonal origin. The identity of the HaCaT line with the tissue of origin was proven by DNA fingerprinting using hypervariable minisatellite probes. This is the first demonstration that the DNA fingerprint pattern is unaffected by long-term cultivation, transformation, and multiple chromosomal alterations, thereby offering a unique possibility for unequivocal identification of human cell lines. The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation.

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