scholarly journals CHARACTERISTICS OF MULTIDRUG RESISTANCE IN HUMAN SKIN MELANOMA CELL LINES

2015 ◽  
Vol 14 (4) ◽  
pp. 39-44
Author(s):  
D. A. Afanasieva ◽  
M. A. Baryshnikova ◽  
T. N. Zabotina ◽  
A. A. Borunova ◽  
O. S. Burova ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Line ◽  
Human Skin ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Mdr1 Gene ◽  
Multi Drug Resistance ◽  
Cytotoxic Action ◽  
Skin Melanoma ◽  
Melanoma Cell Lines

MDR is the main obstacle to chemotherapy efficiency. MDR can grow in cancer cells even if only the one cytostatic agent will act. The aim of the nowadays work is to characterize MDR in metastatic human skin melanoma cell lines prepared in “N.N. Blokhin Russian Cancer Research Center”. pgpl70 expression was detected by immunofluorescence methods. mRNA of MDR gene was identified by Reverse Transcriptase- PCR( RT-PCR) method. Rhodamine 123 (Rhl23) emission has been evaluated by flow cyto- fluorimetiy, cytotoxic activity was estimated by MTT-tests. The cells sensitivity to Aianoza cytostatic effects has showed that mel Kor cells were sensitive to Aranoza acting, but mel Ibr and mel Mtp X were not. Mel Ibr cells had expressed pgpl70 from 35 to 50 per cent, it was detected by immunofluorescence reaction. Mel Kor and mel Mtp-X cells were not expressed P-glycoprotein. mRNA of genes responsible for multi-drug resistance - MDR1, BCRP, MRP1 and LRP (MVP) - were detected by PCR. mRNA of BCRP and MRP1 genes has low expression, barely visible stripes after 33 cycles in all cell lines samples. LRP (MVP) genes expression of mRNA, unfortunately, never managed to see. YB1 gene mRNA expression is well, it is typically for cancer cells. mRNA of gene was found in mel MtpX and mel Ibr subclones cell lines. Mel Kor cells didn't contain mRNA of MDR1 gene. The study of the Rhl23 emission from cells showed that mel Kor control cells had accumulated Rhl23 and didn't throw it out. Mel Ibr cell line accumulated Rhl23 and threw out the half part of it. Mel MtpX cell tine had accumulated the less part of Rhl23 and almost all were thrown out. Thus, the study shows that mel Kor cell tine that are sensitive to Aranoza doesn't express pgpl70, not contain mRNA of multi-chug resistance genes and does not throw Rhl23. Mel Ibr cells resistant to the Aranoza cytotoxic action express pgpl70 ,contain mRNA of MDR1 gene and throw out Rhl23. However, mel MtpX cell line resistant to Aranoza does not express pgpl70, but contains mRNA of MDR1 gene and actively throws out Rhl23.

scholarly journals Adam-9 expression and regulation in human skin melanoma and melanoma cell lines

2005 ◽  
Vol 116 (6) ◽  
pp. 853-859 ◽  
Author(s):  
Paola Zigrino ◽  
Cornelia Mauch ◽  
Jay W. Fox ◽  
Roswitha Nischt
Keyword(s):  
Human Skin ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Skin Melanoma ◽  
Melanoma Cell Lines

scholarly journals In vitro cell cytotoxicity profile and morphological response to polyoxometalate-stabilised gold nanoparticles

2016 ◽  
Vol 40 (2) ◽  
pp. 1039-1047 ◽  
Author(s):  
Isabel Maicas Gabas ◽  
Grazyna Stepien ◽  
María Moros ◽  
Scott G. Mitchell ◽  
Jesús M. de la Fuente
Keyword(s):  
Gold Nanoparticles ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Cell Cytotoxicity ◽  
Skin Melanoma ◽  
Morphological Response ◽  
Antiproliferative Action ◽  
Melanoma Cell Lines

Polyoxometalate-stabilised gold nanoparticles internalise in vast quantities into kidney epithelial and skin melanoma cell lines causing antiproliferative action on tumoural cells.

Secretion of Cytokines by Human Choroidal Melanoma Cells and Skin Melanoma Cell Lines in vitro

1998 ◽  
Vol 30 (3) ◽  
pp. 189-194 ◽  
Author(s):  
Volker Enzmann ◽  
Frank Faude ◽  
Leon Kohen ◽  
Peter Wiedemann
Keyword(s):  
Cell Lines ◽  
Melanoma Cell ◽  
Choroidal Melanoma ◽  
Melanoma Cells ◽  
Skin Melanoma ◽  
Melanoma Cell Lines

scholarly journals Temporal Quantitative Proteomics Reveals Proteomic and Phosphoproteomic Alterations Associated with Adaptive Response to Hypoxia in Melanoma Cells

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2175
Author(s):  
Keshava K. Datta ◽  
Parthiban Periasamy ◽  
Sonali V. Mohan ◽  
Rebekah Ziegman ◽  
Harsha Gowda
Keyword(s):  
Protein Expression ◽  
Cell Survival ◽  
Cancer Cells ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Adaptive Response ◽  
Quantitative Proteomics ◽  
Cellular Response ◽  
Metabolic Reprogramming ◽  
Melanoma Cell Lines

Hypoxia is a common feature in various solid tumours, including melanoma. Cancer cells in hypoxic environments are resistant to both chemotherapy and radiation. Hypoxia is also associated with immune suppression. Identification of proteins and pathways that regulate cancer cell survival in hypoxic environments can reveal potential vulnerabilities that can be exploited to improve the efficacy of anticancer therapies. We carried out temporal proteomic and phosphoproteomic profiling in melanoma cell lines to identify hypoxia-induced protein expression and phosphorylation changes. By employing a TMT-based quantitative proteomics strategy, we report the identification and quantitation of >7000 proteins and >10,000 phosphosites in melanoma cell lines grown in hypoxia. Proteomics data show metabolic reprogramming as one of the prominent adaptive responses in hypoxia. We identify several novel hypoxia-mediated phosphorylation changes that have not been reported before. They reveal kinase signalling pathways that are potentially involved in modulating cellular response to hypoxia. In addition to known protein expression changes, we identify several novel proteomic alterations associated with adaptive response to hypoxia. We show that cancer cells require the ubiquitin–proteasome system to survive in both normoxia and hypoxia. Inhibition of proteasome activity affects cell survival and may provide a novel therapeutic avenue to target cancer cells in hypoxia. Our study can serve as a valuable resource to pursue novel candidates to target hypoxia in cancers and improve the efficacy of anticancer therapies.

Synthesis of N-aryl-2,6-diphenyl-2H-pyrazolo[4,3-c]pyridin-7-amines and their photodynamic properties in the human skin melanoma cell line G361

2021 ◽  
pp. 105570
Author(s):  
Beatričė Razmienė ◽  
Veronika Vojáčková ◽  
Eva Řezníčková ◽  
Lukáš Malina ◽  
Vaida Dambrauskienė ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Skin ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Skin Melanoma

scholarly journals Targeting Mitochondria in Melanoma

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1395
Author(s):  
Sepideh Aminzadeh-Gohari ◽  
Daniela D. Weber ◽  
Luca Catalano ◽  
René G. Feichtinger ◽  
Barbara Kofler ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Mitochondrial Respiration ◽  
Melanoma Cells ◽  
Dermal Fibroblasts ◽  
Glycolytic Activity ◽  
Anti Cancer ◽  
Cancer Agent ◽  
Melanoma Cell Lines

Drastically elevated glycolytic activity is a prominent metabolic feature of cancer cells. Until recently it was thought that tumor cells shift their entire energy production from oxidative phosphorylation (OXPHOS) to glycolysis. However, new evidence indicates that many cancer cells still have functional OXPHOS, despite their increased reliance on glycolysis. Growing pre-clinical and clinical evidence suggests that targeting mitochondrial metabolism has anti-cancer effects. Here, we analyzed mitochondrial respiration and the amount and activity of OXPHOS complexes in four melanoma cell lines and normal human dermal fibroblasts (HDFs) by Seahorse real-time cell metabolic analysis, immunoblotting, and spectrophotometry. We also tested three clinically approved antibiotics, one anti-parasitic drug (pyrvinium pamoate), and a novel anti-cancer agent (ONC212) for effects on mitochondrial respiration and proliferation of melanoma cells and HDFs. We found that three of the four melanoma cell lines have elevated glycolysis as well as OXPHOS, but contain dysfunctional mitochondria. The antibiotics produced different effects on the melanoma cells and HDFs. The anti-parasitic drug strongly inhibited respiration and proliferation of both the melanoma cells and HDFs. ONC212 reduced respiration in melanoma cells and HDFs, and inhibited the proliferation of melanoma cells. Our findings highlight ONC212 as a promising drug for targeting mitochondrial respiration in cancer.

Synthesis and photodynamic properties of pyrazole-indole hybrids in the human skin melanoma cell line G361

2020 ◽  
Vol 183 ◽  
pp. 108666
Author(s):  
Gabrielė Varvuolytė ◽  
Lukáš Malina ◽  
Aurimas Bieliauskas ◽  
Barbora Hošíková ◽  
Helena Simerská ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Skin ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Skin Melanoma
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