To find a cheap and effective hydrogen evolution reaction catalyst, a series of Ni-doped MoS2 microspheres consisting of MoS2 nanosheets have been prepared via hydrothermal synthesis and characterized by scanning electron microscopy (SEM), and X-ray powder diffraction (XRD). Their performance as electrochemical hydrogen evolution reaction (HER) catalyst was studied. It is found that the Ni-doped MoS2 exhibited superior electrocatalytic activity in the HER to undoped MoS2 catalysts. The overpotential of Ni-doped MoS2 has decreased compared with that of undoped sample, indicating an improved activity in HER. Furthermore, the cathodic current for the Ni-doped MoS2 catalyst is about 9 times as much as that for undoped MoS2. Based on our findings, the resulting Ni-doped MoS2 might be promising inexpensive alternative to platinum catalysts in hydrogen production.