Biocompatibility of electrospun cell culture scaffolds made from balangu seed mucilage/PVA composites

2021 ◽  
Author(s):  
alireza allafchian ◽  
Shiva Saeedi ◽  
Seyed Amir Hossein Jalali
Keyword(s):  
Cell Culture ◽  
Chemical Interaction ◽  
Electrospun Nanofibers ◽  
Vero Cells ◽  
Amorphous Structure ◽  
Tension Tests ◽  
Cell Layers ◽  
Major Chemical ◽  
Pva Solutions ◽  
Seed Mucilage

Abstract Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds. Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers. Different BSM/PVA ratios were fabricated to achieve the desired nanofibrous structure for cell proliferation. It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth. The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests. According to the thermal analysis (TGA/DSC), incorporation of PVA enhanced thermal stability of the samples. Successful fabrication of the nanofibers is verified by FT-IR spectra, where no major chemical interaction between BSM and PVA is detected. The crystallinity of the electrospun nanofibers is investigated by XRD, revealing the nearly amorphous structure of BSM/PVA scaffolds. The MTT assay is employed to verify the biocompatibility of the scaffolds. The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.

scholarly journals Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Azmir Arifin ◽  
Maizirwan Mel ◽  
Mohamed Ismail Abdul Karim ◽  
Aini Ideris
Keyword(s):  
Cell Culture ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
High Speed ◽  
Virus Titer ◽  
Vero Cells ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Maximum Cell

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was1.93×106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about7.95×105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.

Mycobacterium abscessus Infection in an Owl Monkey (Aotus trivirgatus)

1970 ◽  
Vol 7 (5) ◽  
pp. 448-454 ◽  
Author(s):  
Alfred G. Karlson ◽  
Herman R. Seibold ◽  
Robert H. Wolf
Keyword(s):  
Cell Culture ◽  
Vero Cell ◽  
Intraperitoneal Injection ◽  
Herpes Virus ◽  
Vero Cells ◽  
Mycobacterium Abscessus ◽  
Vero Cell Culture ◽  
Owl Monkey ◽  
Herpès Virus

Mycobacterium abscessus was isolated from the lungs of an owl monkey which died 27 days after intraperitoneal injection of herpes virus-infected Vero cells. The lungs and liver had multiple microscopic granulomas with acid-fast microorganisms. The mycobacteria also were isolated from a Vero-cell culture inoculated with a suspension of lung and liver. The same microorganism was eventually isolated from Vero cells of the same source as that used to propagate the herpes virus for the original attempt to infect the monkey.

scholarly journals Biocompatible biodegradable polycaprolactone/basil seed mucilage scaffold for cell culture

2018 ◽  
Vol 12 (8) ◽  
pp. 1108-1113 ◽  
Author(s):  
Ali Reza Allafchian ◽  
Seyed Amir Hossein Jalali ◽  
Seyed Ebrahim Mousavi
Keyword(s):  
Cell Culture ◽  
Seed Mucilage

Large-Scale Culture of Vero Cells on GT-2 Microcarrier in Cell Culture Bioreactors

1992 ◽  
pp. 347-349
Author(s):  
Chen Yinliang ◽  
Dong Shupei ◽  
Gu Xiaohua ◽  
Yan Chun ◽  
Song Jiali ◽  
...  
Keyword(s):  
Cell Culture ◽  
Large Scale ◽  
Vero Cells ◽  
Large Scale Culture

Micro cell culture method for determination of diphtheria toxin and antitoxin titres using VERO cells

1974 ◽  
Vol 2 (3) ◽  
pp. 203-209 ◽  
Author(s):  
Kikuko Miyamura ◽  
Etsuko Tajiri ◽  
A. Ito ◽  
R. Murata ◽  
R. Kono
Keyword(s):  
Cell Culture ◽  
Diphtheria Toxin ◽  
Culture Method ◽  
Vero Cells

scholarly journals Identification of Contaminated Cells with Viruses, Bacteria, or Fungi by Fourier Transform Infrared Microspectroscopy

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
V. Erukhimovitch ◽  
M. Huleihil ◽  
M. Huleihel
Keyword(s):  
Cell Culture ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Vero Cells ◽  
Ftir Microscopy ◽  
E Coli ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
Simplex Virus

Fourier transform infrared microspectroscopy (FTIR-M) can detect small molecular changes in cells and therefore was previously applied for the identification of different biological samples. In the present study, FTIR spectroscopy was used for the identification and discrimination of Vero cells infected with herpes viruses or contaminated with bacteria or fungi in cell culture. Vero cells in culture were infected herpes simplex virus type 1 (HSV-1) or contaminated withE. colibacteria orCandida albicansfungi and analyzed by FTIR microscopy at 24 h postinfection/contamination. Specific different spectral changes were observed according to the infecting or contaminating agent. For instance, both pure fungi and cell culture contaminated with this fungi showed specific peaks at 1030 cm−1and at 1373 cm−1regions, while pureE. coliand cell culture contaminated with this bacteria showed a specific and unique peak at 1657 cm−1. These results support the potential of developing FTIR microspectroscopy as a simple, reagent free method for identification and discrimination between different tissue infection or contamination with various pathogens.

Microcarrier cell culture: Vero cells on cytodex® 1

1986 ◽  
Vol 9 (4) ◽  
pp. 205-210
Author(s):  
U. Lindskog ◽  
B. Lundgren ◽  
I. Wergeland ◽  
D. Billig
Keyword(s):  
Cell Culture ◽  
Vero Cells ◽  
Microcarrier Cell Culture

scholarly journals Retinoic acid improves baseline barrier function and attenuates TNF-α-induced barrier leak in human bronchial epithelial cell culture model, 16HBE 14o-

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0242536
Author(s):  
Patrick J. Callaghan ◽  
Elizabeth Rybakovsky ◽  
Bryan Ferrick ◽  
Sunil Thomas ◽  
James M. Mullin
Keyword(s):  
Cell Culture ◽  
Retinoic Acid ◽  
Barrier Function ◽  
Bronchial Epithelial Cell ◽  
Cell Culture Model ◽  
Epithelial Cell Culture ◽  
Bronchial Epithelial ◽  
Culture Model ◽  
Tnf Α ◽  
Cell Layers

Retinoic acid (RA) has been shown to improve epithelial and endothelial barrier function and development and even suppress damage inflicted by inflammation on these barriers through regulating immune cell activity. This paper thus sought to determine whether RA could improve baseline barrier function and attenuate TNF-α-induced barrier leak in the human bronchial epithelial cell culture model, 16HBE14o- (16HBE). We show for the first time that RA increases baseline barrier function of these cell layers indicated by an 89% increase in transepithelial electrical resistance (TER) and 22% decrease in 14C-mannitol flux. A simultaneous, RA-induced 70% increase in claudin-4 attests to RA affecting the tight junctional (TJ) complex itself. RA was also effective in alleviating TNF-α-induced 16HBE barrier leak, attenuating 60% of the TNF-α-induced leak to 14C-mannitol and 80% of the leak to 14C-inulin. Interleukin-6-induced barrier leak was also reduced by RA. Treatment of 16HBE cell layers with TNF-α resulted in dramatic decrease in immunostaining for occludin and claudin-4, as well as a downward “band-shift” in occludin Western immunoblots. The presence of RA partially reversed TNF-α’s effects on these select TJ proteins. Lastly, RA completely abrogated the TNF-α-induced increase in ERK-1,2 phosphorylation without significantly decreasing the TNF-driven increase in total ERK-1,2. This study suggests RA could be effective as a prophylactic agent in minimizing airway barrier leak and as a therapeutic in preventing leak triggered by inflammatory cascades. Given the growing literature suggesting a “cytokine storm” may be related to COVID-19 morbidity, RA may be a useful adjuvant for use with anti-viral therapies.

scholarly journals Genetic relationships of Corynebacterium diphtheriae strains isolated from a diphtheria case and carriers by restriction fragment length polymorphism of rRNA genes

1995 ◽  
Vol 37 (4) ◽  
pp. 291-296
Author(s):  
Claudio Tavares Sacchi ◽  
Ana Paula Silva de Lemos ◽  
Silvana Tadeu Casagrande ◽  
Alice Massumi Mori ◽  
Carmecy Lopes de Almeida
Keyword(s):  
Cell Culture ◽  
Genetic Relationships ◽  
Culture Method ◽  
Vero Cells ◽  
Corynebacterium Diphtheriae ◽  
Rrna Genes ◽  
Diffusion Method ◽  
Rrna Gene

In the present study we report the results of an analysis, based on ribotyping of Corynebacterium diphtheriae intermedius strains isolated from a 9 years old child with clinical diphtheria and his 5 contacts. Quantitative analysis of RFLPs of rRNA was used to determine relatedness of these 7 C.diphtheriae strains providing support data in the diphtheria epidemiology. We have also tested those strains for toxigenicity in vitro by using the Elek's gel diffusion method and in vivo by using cell culture method on cultured monkey kidney cell (VERO cells). The hybridization results revealed that the 5 C.diphtheriae strains isolated from contacts and one isolated from the clinical case (nose case strain) had identical RFLP patterns with all 4 restriction endonucleases used, ribotype B. The genetic distance from this ribotype and ribotype A (throat case strain), that we initially assumed to be responsible for the illness of the patient, was of 0.450 showing poor genetic correlation among these two ribotypes. We found no significant differences concerned to the toxin production by using the cell culture method. In conclusion, the use of RFLPs of rRNA gene was successful in detecting minor differences in closely related toxigenic C.diphtheriae intermedius strains and providing information about genetic relationships among them.

scholarly journals Complete Genome Sequences, before and after Mammalian Cell Culture, of Zika Virus Isolated from the Serum of a Symptomatic Male Patient from Oaxaca, Mexico

2017 ◽  
Vol 5 (12) ◽  
Author(s):  
Celia Boukadida ◽  
Jesús M. Torres-Flores ◽  
Martha Yocupicio-Monroy ◽  
Elvira Piten-Isidro ◽  
Amaranta Y. Rivero-Arrieta ◽  
...  
Keyword(s):  
Cell Culture ◽  
Male Patient ◽  
Zika Virus ◽  
Mammalian Cell Culture ◽  
Vero Cells ◽  
Neurological Complications ◽  
Nucleotide Polymorphisms ◽  
Limited Information ◽  
Viral Genomes ◽  
Before And After

ABSTRACT Zika virus (ZIKV) is an emerging arthropod-borne flavivirus associated with severe congenital malformations and neurological complications. Although the ZIKV genome is well characterized, there is limited information regarding changes after cell isolation and culture adaptation. We isolated, and passaged in Vero cells, ZIKV from the serum of a symptomatic male patient and compared the viral genomes before and after culture. Single nucleotide polymorphisms were characteristic among serum-circulating genomes, while such diversity decreased after cell culture.

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