Test of Antibacterial Activity 70% Ethanol Extract of Seeds Black Pepper (Piper Nigrum L) Against Bacteria Escherichia Coli Atcc 25922

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn) on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

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AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Sinularia SP. DI TELUK MANADO

PHARMACON ◽

10.35799/pha.8.2019.29244 ◽

2019 ◽

Vol 8 (1) ◽

pp. 114

Author(s):

Lyone Katiandagho ◽

Defny S Wewengkang ◽

Sri Sudewi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Soft Coral ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Chloroform Fraction ◽

Water Fraction

ABSTRACT Soft Coral Sinularia sp.has been shown to have antibacterial activity. This research aims to determine the antibacterial activity of marine organisms obtained from Manado bay. The method in this study is the diffusion method with positive control chloramphenicol and negative control of methanol in Escerichia coli and Sthaphylococcus aureus. The result showed that extracts, chloroform fraction and methanol-water fraction were obtained inhibiting category of Sthapylococcus aureus bacteria, but in the chloroform fraction and ethanol extract can inhibit Escerichia coli bacteria categorized as strong. With it can be conlueded that the extract and fraction of soft Sinularia sp. have bioactive compounds with a broth spectrum of antibacterial activity.Keywords: Sinularia sp., Chloramphenicol, Escerichia coli, Sthaphylococcus aureus, and antibacterial. ABSTRAK Karang lunak Sinularia sp. telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri pada organisme biota laut yang diperoleh dari teluk Manado. Metode dalam penelitian ini adalah metode difusi agar dengan kontrol positif kloramfenikol dan kontrol negatit metanol pada Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak, fraksi kloroform, dan fraksi metanol-air dapat menghambat bakteri Staphylococcus aereus dikategorikan sedang, namun pada fraksi kloroform dan ekstrak etanol dapat menghambat bakteri Escerichia coli dikategorikan kuat. Dengan demikian dapat disimpulkan bahwa ekstrak dan fraksi karang lunak Sinularia sp.memiliki senyawa bioaktif dengan spektrum yang luas terhadap aktivitas antibakteri.Kata kunci: Sinularia sp, Kloramfenikol, Escherichia coli, Staphylococcus aureus, dan antibakteri

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UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽

10.33369/atp.v3i2.10122 ◽

2019 ◽

Vol 3 (2) ◽

Author(s):

Deza Oktasila ◽

Nurhamidah Nurhamidah ◽

Dewi Handayani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oil ◽

Ethanol Extract ◽

Inhibition Zone ◽

Raw Material ◽

Diffusion Method ◽

Citrus Leaves ◽

Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10; and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is 7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

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SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

Journal of Pharmacopolium ◽

10.36465/jop.v1i1.394 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

Vera Nurviana

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Diffusion Method ◽

Water Fraction ◽

Tetracycline Hcl

Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl. Keywords: Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.

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Aktivitas Antibakteri Ekstrak Jamur Lingzhi (Ganoderma lucidum) Menggunakan Pelarut Etanol terhadap Escherichia coli.

Journal of Pharmacy and Science ◽

10.53342/pharmasci.v2i1.64 ◽

2017 ◽

Vol 2 (1) ◽

pp. 33-35

Author(s):

Prasetyo Handrianto

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Ganoderma Lucidum ◽

Herbal Remedy ◽

Transmembrane Protein ◽

Ethanol Extract ◽

Inhibition Zone ◽

Clear Zone ◽

Porin Protein ◽

Paper Disc

ABSTRAKJamur Lingzhi (Ganoderma lucidum) merupakan herbal yang memiliki aktivitas antibiotik atau antibakteri. Triterpenoid dalam Jamur Lingzhi mengandung senyawa yang menunjukkan aktivitas antibakteri melaluimekanisme pengikatan polimer yang kuat terhadap porin (protein transmembran) pada membran luar dari dinding sel bakteri sehingga menyebabkan kerusakan pada porin. Tujuan penelitian ini adalah menentukan efek ekstrak etanol Jamur Lingzhi dalam menghambat pertumbuhan bakteri Escherichia coli. Metode yang digunakan adalah metode cakram dengan mengamati zona hambat yang muncul disekitar cakram. Kosentrasi ekstrak yang digunakan adalah 20 µg / ml, 40 µg / ml, 60 µg / ml, 80 µg / ml, 100 µg / ml. Kosentrasi ekstrak 20µg / ml dan 40 µg / ml menghasilkan zona hambat masing-masing 10,5 mm dan 12,4 mm yang dikategorikan sebagai kurang aktif, sedangkan kosentrasi 60 µg / ml, 80 µg / ml, dan 100 µg / ml menghasilkan zona hambat 13,65 mm, 14,9 mm, dan 16,5 mm yang dikategorikan sebagai aktif. Hasil menunjukkan ekstrak etanol Jamur Lingzhi dapat menghambat pertumbuhan bakteri Escherichia coli.Kata kunci : Ganoderma lucidum, Escherichia coli, Ekstrak etanol, Antibakteri.ABSTRACTLingzhi mushroom (Ganoderma lucidum) is a herbal remedy that has as an antibiotic or antibacterial activity. Lingzhi mushroom triterpenoids containing compounds which possess antibacterial activity by reacting with porin mechanisms (transmembrane protein) on the outer membrane of the bacterial cell wall, forming a strong bond polymers that cause damage porin. The purpose of this study was to was to determine the effect of Lingzhi mushroom extract (Ganoderma lucidum) using ethanol in inhibiting the growth of bacteria Escherichia coli. The method used was paper disc method by observing the clear zone that is visible around the paper disc. And concentration used was 20 µg / ml, 40 µg / ml, 60 µg / ml, 80 µg / ml, 100 µg / ml. At a concentration of 20 µg /ml and 40 µg / ml produce inhibition zone of 10.5 mm and 12.4 mm by category inhibition zone less active and at a concentration of 60 µg / ml, 80 µg / ml, 100 µg / ml produce inhibition zone 13.65 mm by 14.9 mm and 16.5 mm in the active category. The results obtained show that the extract of lingzhi mushroom (Ganoderma lucidum) using ethanol effect on the growth of the bacterium Escherichia coli.Keywords: Ganoderma lucidum , Escherichia coli, Ethanol extract, Antibacterial.

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PENETAPAN KADAR PIPERIN DALAM EKSTRAK BUAH LADA HITAM (Piper nigrum Linn.) MENGGUNAKAN LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC–MS/MS)

Jurnal Ilmiah Farmasi Farmasyifa ◽

10.29313/jiff.v1i2.3160 ◽

2018 ◽

Vol 1 (2) ◽

pp. 69-79

Author(s):

Alifia Putri Febriyanti ◽

Siti Jazimah Iswarin ◽

Susanti Susanti

Keyword(s):

Quantitative Analysis ◽

Ethanol Extract ◽

Black Pepper ◽

Piper Nigrum ◽

Value Recovery ◽

Pepper Fruit ◽

Validation Parameters ◽

Liquid Chromatography Tandem Mass ◽

Layer Chromatography ◽

Dark Blue

The utilization of medicinal plants have started growing rapidly in the world, including in Indonesia, along with the thinking back to nature, one of the plants that are often used as a medicine is black pepper fruit (Piper nigrum Linn.). Piperine is a major compound and potent substances contained in black pepper fruit as antidiarrheal activity. The aim of this study was to determine levels of piperine in 96% ethanol extract of black pepper fruit. The extraction method used Soxhlet in 96% ethanol as solvent. Analysis of piperine using qualitative analysis by thin layer chromatography (TLC) produces yellow stain after being sprayed by dragendorf and dark blue on observation Uv-Vis 365 nm with Rf extract value 0.49 and Rf piperine value 0.5. Quantitative analysis using LC – MS produces piperine levels at 26%. Before quantitative analysis, validation method needs to be done with the parameters, among others: linearity have regression equation y = has 1,981,691.1333x + 561,445.0000 and correlation coefficient (R2) of 0.9973, LOD and LOQ at 0.14 and 0.44 ppm, accuracy value (% recovery) between 95.90 – 100.77%, precision value (% KV) between 0.02 – 1.84%, and selectivity (RT) between 2.47 – 2.49. The results of the validation parameters are eligible so that piperine levels were obtained by LC – MS declared accurate, specific, and precise.

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Antibacterial Activity of Dacryodes edulis Seed Extracts on Food-Borne Pathogens

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v4i1.3 ◽

1970 ◽

Vol 4 (1) ◽

pp. 17-21 ◽

Cited By ~ 3

Author(s):

BA Omogbai ◽

TO Eneh

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Pharmaceutical Preparation ◽

Water Extract ◽

Ethanol Extract ◽

Antibacterial Activities ◽

Food Borne ◽

Dacryodes Edulis ◽

Seed Extracts

The antibacterial effect of aqueous and ethanolic seed extracts of Dacryodes edulis was studied using agar diffusion technique. Extracts tested at a final concentration 2000;g/ml produced in vitro antibacterial activities in assays against food-borne bacterial isolates of Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes, Pseudomonas aeruginosa, Salmonella typhi and Proteus mirabilis. The highest zone of inhibition was obtained from ethanol extract at 2000;g/ml against Escherichia coli with a diameter of 28mm.The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the water extract ranged between 62.5-500;g/ml and 125-1000;g/ml respectively. The MIC and MBC of the ethanol extract ranged between 31.25-250;g/ml and 62.5-500;g/ml. The percentage inhibition of the isolates at 1000;g/ml was more in the ethanol extract (65.4-95.5%) compared to the water extract (30-2-60.2%). This study shows that the extracts of Dacryodes edulis have potential usage as a food preservative and pharmaceutical preparation. Keywords: Dacryodes edulis, Antibacterial activity, Aqueous, Ethanolic, Seed extract

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Effects of Black Pepper (Piper nigrum L.) Chloroform Extract on the Enzymatic Activity and Metabolism of Escherichia coli and Staphylococcus aureus

Journal of Food Quality ◽

10.1155/2018/9635184 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Wenxue Chen ◽

Lan Zou ◽

Weijun Chen ◽

Yueying Hu ◽

Haiming Chen

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Bacterial Cell ◽

Cell Walls ◽

Antibacterial Activities ◽

Black Pepper ◽

Chloroform Extract ◽

Piper Nigrum ◽

Bacterial Cells ◽

Bacterial Cell Walls

The chemical composition and antimicrobial mechanism of action of black pepper chloroform extract (BPCE) were investigated, as well as the potential antibacterial activities of BPCE against Escherichia coli and Staphylococcus aureus. The results showed that 1H-Cycloprop[e]azulen-7-ol, decahydro-1,1,7-trimethyl-4-methylene-, [1ar-(1aα,4aα,7β,7a,β,7bα.)]- (8.39%) and 2-methylene-4,8,8-trimethyl-4-vinyl-bicyclo[5.2.0]nonane (6.92%) were identified as the two primary components of BPCE. The release of intracellular transaminases from bacteria after being incubated with BPCE revealed that the bacterial cell walls and membranes were degraded and that protein synthesis was inhibited to some extent. The inhibition of bacterial Na+/K+-ATPase activity upon the addition of BPCE also indicated an enhanced permeability of bacterial cell membranes. Moreover, an analysis of hexokinase and pyruvate kinase activities showed that BPCE affected the metabolic rate of glycolysis and disrupted the normal metabolism of bacteria. This phenomenon was supported by an observed accumulation of lactic acid (LA) in the treated bacterial cells. Overall, our results indicated that BPCE damaged bacterial cell walls and membranes, which was followed by a disruption of bacterial cell respiration.

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Influence of Magnetic Field on Antibacterial Effect of Extracts from Black Pepper

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1677 ◽

2012 ◽

Vol 550-553 ◽

pp. 1677-1680

Author(s):

Wen Xue Chen ◽

Jiao Mei Li ◽

Mei Fang Peng ◽

Song Hai Wu ◽

Hou Yuan Qiu

Keyword(s):

Magnetic Field ◽

Escherichia Coli ◽

Antibacterial Activity ◽

Aspergillus Niger ◽

Antibacterial Effect ◽

Inhibition Zone ◽

Black Pepper ◽

Indicator Bacteria ◽

Ethanol Extraction ◽

Ultrasonic Assisted

In order to investigate the effect of magnetic field on the antibacterial substances from black pepper, we used shaking and ultrasonic-assisted ethanol extraction with Escherichia coli, Staphylococcus aureus, Bacillus anthracis and Aspergillus niger as indicator bacteria respectively. Meanwhile, extraction rate and diameter of inhibition zone were regarded as the activity monitoring parameters. The results showed the existence of magnetic field can make a difference. It weakened the antibacterial activity of black pepper by shaking extraction. However, the antibacterial activity of substances to Aspergillus niger was proportional to the intensity of the applied magnetic field by ultrasonic-assisted ethanol extraction.

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