scholarly journals PENETAPAN KADAR PIPERIN DALAM EKSTRAK BUAH LADA HITAM (Piper nigrum Linn.) MENGGUNAKAN LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC–MS/MS)

2018 ◽  
Vol 1 (2) ◽  
pp. 69-79
Author(s):  
Alifia Putri Febriyanti ◽  
Siti Jazimah Iswarin ◽  
Susanti Susanti
Keyword(s):  
Quantitative Analysis ◽  
Ethanol Extract ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Value Recovery ◽  
Pepper Fruit ◽  
Validation Parameters ◽  
Liquid Chromatography Tandem Mass ◽  
Layer Chromatography ◽  
Dark Blue

The utilization of medicinal plants have started growing rapidly in the world, including in Indonesia, along with the thinking back to nature, one of the plants that are often used as a medicine is black pepper fruit (Piper nigrum Linn.). Piperine is a major compound and potent substances contained in black pepper fruit as antidiarrheal activity. The aim of this study was to determine levels of piperine in 96% ethanol extract of black pepper fruit. The extraction method used Soxhlet in 96% ethanol as solvent. Analysis of piperine using qualitative analysis by thin layer chromatography (TLC) produces yellow stain after being sprayed by dragendorf and dark blue on observation Uv-Vis 365 nm with Rf extract value 0.49 and Rf piperine value 0.5. Quantitative analysis using LC – MS produces piperine levels at 26%. Before quantitative analysis, validation method needs to be done with the parameters, among others: linearity have regression equation y = has 1,981,691.1333x + 561,445.0000 and correlation coefficient (R2) of 0.9973, LOD and LOQ at 0.14 and 0.44 ppm, accuracy value (% recovery) between 95.90 – 100.77%, precision value (% KV) between 0.02 – 1.84%, and selectivity (RT) between 2.47 – 2.49. The results of the validation parameters are eligible so that piperine levels were obtained by LC – MS declared accurate, specific, and precise.

scholarly journals Piperine Levels in Java Chili and Black Fruits Extracts from Regions with Different Altitude

2021 ◽  
Vol 6 (1) ◽  
pp. 16-22
Author(s):  
Ni Putu Ermi Hikmawanti ◽  
Endang Hanani ◽  
Shintia Maharani ◽  
Ajeng Istiningtyas Wahyudi Putri
Keyword(s):  
High Altitude ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Pepper Fruit ◽  
Uv Visible ◽  
Low Altitude ◽  
Piper Retrofractum ◽  
Layer Chromatography

Piper retrofractum Vahl. (java chili or cabe jawa) and Piper nigrum L. (black pepper or lada hitam) are parts of the Piperaceae. One of the chemical constituents found in the fruit of the two plants is piperine, which has properties as antimicrobial, antidiabetic, anti-inflammatory, anticancer etc. This study aims to determine the piperine levels contained in 95% ethanol extract of java chili and black pepper fruits from regions with different altitude using UV-Visible spectrophotometer. Each sample were extracted by maceration method using 95% ethanol. Qualitative identification of piperine was carried out using thin layer chromatography (TLC). Determination of piperine levels was carried out with UV-Visible spectrophotometer at maximum wavelength of 253.8 nm. The results showed that the levels of piperine contained in the 95% ethanol extract of java chili fruit from low altitude (Lampung), medium altitude (Madura) and high altitude (Bogor) are 1.54±0.02%; 1.44±0.02%; and 1.41±0.02% (w/v), respectively. Whereas the levels of piperine contained in the 95% ethanol extract of black pepper fruit from low altitude (East Luwu), medium altitude (Central Lampung) and high altitude (Bogor) are 5.63±0.14%; 5.38±0.05%; and 4.87±0.09% (w/v), respectively.

Pharmacognostic evaluation and instrumental analysis (SEM) for the standardization of Piper nigrum L., (black pepper) fruit

2019 ◽  
Vol 51 (5) ◽  
Author(s):  
Najma Shaheen ◽  
Shahlla Imam ◽  
Rafi Akhter Sultan ◽  
Safia Abidi ◽  
Iqbal Azhar ◽  
...  
Keyword(s):  
Black Pepper ◽  
Instrumental Analysis ◽  
Piper Nigrum ◽  
Pepper Fruit

scholarly journals EFFECT OF FEEDING TREATMENT WITH SOME EXTRACTS OF BLACK PEPPER ON SOME BIOLOGICAL ASPECTS OF COTTON LEAF WORM

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
AL-Khazraji & et al.
Keyword(s):  
Ethanol Extract ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Cotton Leaf ◽  
Food Treatment ◽  
Malva Sylvestris ◽  
Effect Of Food ◽  
Oil Extract ◽  
Biological Aspects ◽  
Effect Of Feeding

This research was conducted to study the effect of food treatment leaves of Malva sylvestris with ethanol ,ethyl acetate, hexane and oil extract of  black pepper fruits Piper nigrum on some biological aspects of cotton leaf worm Spodoptera littoralis by using the concentrations of 1.25, 2.5 and 5% of each extract, The results of feeding treatments of second larvae instar showed an increase in mortality percentages which reached 100% at the all extracts when using 5% concentrate. used in the test, while the highest mortality reached to 26.7% when using ethanol extract at 5% concentrate for sixth larvae instar fed on treated leaves for 24 hrs., it was also found that the period of larvae growth and pupae were prolonged by ethanol extract which reached to 33 days at the conc. 2.5% for the second larvae instar while reached 25 days in control, and pupae growth reached to 14 days at the same conc. while reached 10.3 days in control.

scholarly journals OPTIMATION OF ETHANOL CONCENTRATION IN EXTRACTION OF EUGENOL FROM GALANGAL RHIZOME

2017 ◽  
Vol 10 (14) ◽  
pp. 18
Author(s):  
Adi Yugatama ◽  
Nur Wahida Ardiyati ◽  
Ira Yulianti
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Thin Layer ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Ethanol Concentration ◽  
Ethanol Extract ◽  
Standard Curve ◽  
Layer Chromatography ◽  
Curve Equation

   Objective: The main objective of this study was to determine the optimal ethanol concentration as solvent for extracting eugenol from galangal.Methods: Galangal rhizome extraction was conducted with kinetic maceration at 50°C with various ethanol concentration as solvent (0%, 30%, 50%, 70%, and 96%). A 1:10 ratio of rhizome and ethanol was applied. The extract was obtained, then its eugenol concentration was analyzed qualitatively and quantitatively by thin-layer chromatography-densitometer with n-hexane:ethyl acetate (4:1) by using mobile phase and silica gel 60 F254 as stationary phase and applying a wavelength of 283 nm.Results: The result of qualitative analysis showed that extract of 70% and 96% ethanol had a spot with Rf value 0.63, which was equal to the Rf value of standard eugenol. Standard curve equation for the 70% ethanol extract was y=89318x+656.07 (r=0.9993) and for the 96% ethanol extract was y=8658x+1743 (r=0.9999). The result of the quantitative analysis showed that the 70% and 96% ethanol extract contained 4.85% and 4.79% eugenol, respectively.Conclusion: Extraction of galangal rhizome in 70% and 96% ethanol was positively containing eugenol. Highest eugenol concentration (4.85%) was obtained from galangal rhizome extraction in 70% ethanol.

scholarly journals Studying the Effectiveness of Some Plant Powders on the Biological Activities of the Confused Flour Beetle Tribolium confusum du Val (Coleoptera: Tenebrio-nidae).

2020 ◽  
Vol 35 (2) ◽  
pp. 166-175
Author(s):  
Nagat Ali Abuelnnor
Keyword(s):  
Biological Activities ◽  
Black Pepper ◽  
Tribolium Confusum ◽  
Piper Nigrum ◽  
Flour Beetle ◽  
Pepper Fruit ◽  
Plant Powders ◽  
Leaf Powder ◽  
Myristica Fragrans Houtt ◽  
Confused Flour Beetle

This study aims to test the biological effect of three plant powders of (black pepper fruit Piper nigrum L., nutmeg seeds Myristica fragrans Houtt and bay leaf Laurus nobilis L.) when mixed with wheat flour at concentrations of; 0.2, 0.5, 0.75, 1 and 1.5 % (w/w) against the confused flour beetle Tribolium confusum. Results showed that the black pepper fruit caused a decrease in the production of larvae that reached 65.36%, while the nutmeg seeds powder caused a decrease of 50.93% compared with the bay leaf powder, which caused a decrease of 38.20%. The results also showed a decrease in the first generation progeny (F1) that reached 90.75% and 75.34% due to the treatment with the black pepper fruit and the nutmeg seeds powders respectively. However, the bay leaf powder was less effective, with only a decrease of 30.13%.

Antileishmanial Alkaloids from Black Pepper (Piper nigrum)

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
HRW Dharmaratne ◽  
BL Tekwani ◽  
NPD Nanayakkara
Keyword(s):  
Black Pepper ◽  
Piper Nigrum

DETERMINATION OF THE EFFICIENCY AND EFFICACY OF BIO-CHEMICAL FERTILIZER ON THE SOIL, YIELD AND GROWTH PERFORMANCE OF BLACK PEPPER (Piper nigrum L.)

2015 ◽  
Vol 4 (3) ◽  
pp. 460-468
Author(s):  
Yap Chin Ann
Keyword(s):  
Growth Performance ◽  
Nutrient Management ◽  
High Growth ◽  
Black Pepper ◽  
Chemical Fertilizer ◽  
Piper Nigrum ◽  
High Fertility ◽  
Fertility Level ◽  
Area Index ◽  
K 12

The last nutrient management review of black pepper was done in 1968. There is, therefore, a need to develop new technology to improve pepper production and transfer that technology to production site. This experiment was carried out to study the effect of newly developed biochemical fertilizer on some physiological characteristics, yield and soil fertility of pepper. The treatment consisted of T1 (BS): chemical fertilizer (N:12%, P:12%, K:17%); T2 (BK1): biochemical fertilizer F1 N:15%, P:5%, K:14) and T3 (BK2): biochemical fertilizer F2 (N:13%, P:4%, K:12). The biochemical fertilizer F1 out-yielded chemical and biochemical fertilizer F2 by 75.38% and 16.45% respectively with the higher yield being associated with various phonotypical alterations, which are reported here. Significant measureable changes were observed in physiological processes and plant characteristics, such as large leaf area index, more chlorophyll content and high photosynthesis rate coupled with lower transpiration rate in biochemical fertilizer F1(BK1) treatment compared with other treatment. The high fertility level in biochemical fertilizer F1 and biochemical fertilizer F2 (BK2) reflected the important of organic material in improving soil quality. In conclusion, the achieve high growth performance and yield in pepper, chemical fertilizer alone is insufficient whilst combination of organic and inorganic fertilizer with balance nutrient content gave a significant increase in yield and growth of pepper. 

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