KARAKTERISTIK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIUM DASAR JERAMI DAN TONGKOL JAGUNG

Laju pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus (Jacq) P. Kumm) dengan medium dasar jerami dan tongkol jagung telah dilaksanakan di Laboratorium Mikrobiologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam. Tujuan Penelitian; (a) Untuk mengamati pertumbuhan jamur tiram putih (Pleurotus ostreatus) pada formulasi media limbah jerami dan tongkol jagung; (b) Untuk mengetahui perbandingan dosis limbah jerami dan tongkol jagung yang dapat memberikan pertumbuhan terbaik terhadap jamur tiram putih. Penelitian ini didesain berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari 7 perlakuan dan 3 kali ulangan. Susunan perlakuan merupakan perbandingan antara Serbuk jerami jagung : serbuk Tongkol Jagung yakni P1 (Serbuk Jerami 100%, tanpa Serbuk Tongkol Jagung), P2 (80% + 20%), P3 (60% + 40%), P4 (40% + 60%), P5 (20% + 80%), P6 (Serbuk Tongkol Jagung 100%, tanpa Serbuk jerami), P7 (Serbuk Gergaji 70% + Dedak Padi 20% + Tepung Jagung 10%, tanpa Serbuk Jerami dan Tongkol Jagung). Parameter pengamatan; (a) Media dasar pertumbuhan; (b) Karakteristik mikroskopik hifa; (c) Laju pertumbuhan miselium; (d) Karakteristik makroskopik koloni; (e) Masa inkubasi. Hasil penelitian menunjukkan laju pertumbuhan miselium tercepat pada perlakuan P4 yaitu 103 mm dan terendah pada perlakuan P1 yaitu 75 mm. Jumlah koloni (CFU) tertinggi pada perlakuan P7 yaitu 1,6x1012CFU/g dan terendah pada perlakuan P4 yaitu 0,2x1012CFU/g. Waktu inkubasi paling cepat pada perlakuan P7 yaitu 28 hari dan terendah pada perlakuan P4 yaitu 30 hari.

INVENTARISASI ROTAN DI HUTAN LINDUNG DANAU SELOGAN KAPUAS HULU KALIMANTAN BARAT

Rotan mempunyai manfaat sebagai bahan baku kerajinan seperti keranjang, caping, tikar, nyiru, sapu, dan alat pengikat. Hutan Danau Selogan memiliki lingkungan yang cocok sebagai tempat tumbuhnya tanaman rotan. Penelitian tentang “inventarisasi rotan di hutan lindung danau selogan Kapuas Hulu”, Desa Ujung Said, Kecamatan Jongkong, Kabupaten Kapuas Hulu, Kalimantan Barat, telah dilaksanakan, penelitian ini bertujuan untuk mengetahui jenis-jenis rotan yang ada di hutan lindung Danau Selogan. Metode penelitian ini adalah deskriptif. Sampel diambil menggunakan teknik purposive sampling dengan metode jelajah. Berdasarkan hasil penelitian ditemukan 6 jenis tumbuhan rotan, yaitu Korthalsia rigida Blume, Calamus pasphalantus Beccari, Daemonorops sabut Beccari, Korthalsia junghuhnii Blume, Calamus caesius Blume, dan Korthalsia echinometra Beccari.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn) on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

RESPON IMUN HOSPES TERHADAP INFEKSI Vibrio cholerae

The immune sistem is a way of the body’s defense sistem to save the host from the invasion of outside pathogen. Based on how respon to disease, that differentiated into two immune system are innate and adaptive system. Because it an cant throgh the stomach, these pathogenic bacteria go to the small intestin as a site infection. In the intestine, V. cholerae bactesia adhere and colonize and invasion to intestinal epihelial cells. Protection mechanism to V. cholerae are the natural defense presence of tick mucosa on the surface of epithelial cells can inhibit pathogene to adhere tointestinal epithelial cells. One anothet defense namely innate immune system did by phagocytic cells to attac pathogen agent and adaptive immune system involves IgA to opsonization so that can increase intestinal mucosal immune system

PENGARUH PUPUK HAYATI DENGAN KOMPOSISI MIKROORGANISME YANG BERBEDA TERHADAP PERTUMBUHAN TANAMAN

Biofertilizer can be used to replace chemical fertilizer so that soil quality is maintained and soil pollution can be avoided. The study aimed to determine the effect of biofertilizers with different compositions of microorganisms on the growth of maize plants. The research was carried out in a greenhouse. Maize plants were fertilized by two kinds of biofertilizers with different compositions of microorganisms. The first biofertilizer contained Azotobacter sp, Azospirillum sp, Rhizobium sp, Trichoderma sp, and Lactobacillus sp, and the second biofertilizer contained Strenotrophomonas sp. and Paenibacillus polymyxa. As controls, maize plants were fertilized by sterilized those biofertilizers. The results showed that the second biofertilizer increased the dry weight of the maize plant. Meanwhile, the first biofertilizer did not increase it. Several things must be considered in the application of biofertilizers, such as the composition of microorganisms, type of plant, level of fertilization, and the method of applying fertilizer to the plant.

PENGARUH KOMBINASI IAA (Indole-3-Acetic Acid) DAN BAP (6-Benzylaminopurine) TERHADAP INISIASI TANAMAN VANILI (Vanilla planifolia Andrews)

Provision of Vanilla (Vanilla planifolia Andrews) with traditional cultivation often has problems, including the availability of unhealthy seeds due to disease caused by Fusarium oxysporum. This disease can thwart vanilla plantations up to 85% because the pathogen can infect all parts of the vanilla plant making it difficult to control efforts. Alternative efforts were made to overcome this problem, namely through the initiation process in tissue culture by combining the hormones IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine). This study aims to determine the effect and the maximum concentration of the combination of IAA and BAP on the initiation of vanilla plants. This study was conducted based on a completely randomized design (CRD) with 5 treatments and 3 repetitions. This treatment consisted of: A (MS + 0.1 ppm IAA + 1 ppm BAP), B (MS + 0.2 ppm IAA + 1 ppm BAP), C (MS + 0.3 ppm IAA + 1 ppm BAP), D (MS + 0.4 ppm IAA + 1 ppm BAP), and E (MS + 0.5 ppm IAA + 1 ppm BAP). Observations were made after 40 days from the planting process. The results showed that the concentration of the combination of IAA and BAP can affect the growth and organogenesis of the initiation of vanilla plants (Vanilla planifolia Andrews). Treatment C (MS + 0.3 ppm IAA + 1 ppm BAP) was the best concentration from this study, with average values: number of shoots (1), shoot length (1.73 cm), number of roots (1), and number of leaves (1.33).

IDENTIFICATION OF PLANT UTILIZATION IN THE LIFE CYCLE CEREMONY OF THE KAILI DA'A TRIBE IN UWEMANJE VILLAGE, KINOVARO DISTRICT, SIGI REGENCY

The purpose of this study was to obtain information about the types of plants used by the Kaili Da’a tribe in life cycle ceremonies that are still preserved for generations in Uwemanje village, Kinovaro district, Sigi district. The research population is all people who live in Uwemanje Village, Kinivaro District, which are spread over 4 hamlets. The research method uses survey research with the help of interview questionnaires. Interviews were conducted on key respondents and general respondents. Key respondents were selected by purposive sampling as much as 10% of the population, namely the people who were considered to be the most knowledgeable about the ins and outs of this ceremony, such as Sando, traditional leaders, village heads and village priests. Meanwhile, general respondents are people who are recommended by key respondents with a record that they are at least 30 years old and are married or have a family. The data and information in this study were processed descriptively. Types of plants/plants used in life cycle ceremonies consist of 21 species, namely: rice (Oryza sativa L.), coconut (Cocos nucifera L.), banana (Musa paradisiaca L.), cocor duck (Kalanchoe pinata L.), areca nut (Areca catechu L.), turmeric (Curcuma domestica L.), nail paci (Lawsonia enermis L.), bogenvil (Boganvillea glabra L.), pandanus (Pandanus amarylifolius L.), jatropha (Jatropha curcas), forest basil ( Ocimum sanctum L.), moringa (Moringa oleifera L.), shallot (Allium cepa L.), Bengal grass (Eleusine indica L.), hibiscus (Hibiscus tiliaceus L.), peacock flower (Caesalpinia pulcherima L.), betel (Piper betle L.), keluwih (Artocarpus camansi L.), jasmine (Jasminum sambac L.), palm sugar (Arenga pinnata), and red sugar cane (Saccharum officinarum L.). The percentage of utilization of plant parts that are most directly utilized is the leaves which amount to 14 with a percentage of 65.66%, flowers amount to 4 to 16.67%, fruit to reach 3 to 12.5%. The least used parts are rhizomes, seeds, tubers, roots and stems with a percentage of 5.17%.

POTENSI EKONOMI LIMBAH TANDAN KOSONG KELAPA SAWIT DAN DEDAK PADI SEBAGAI MEDIA TUMBUH MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus)

Penelitian ini perlu di lakukan karena pada limbah tandan kosong kelapa sawit dan dedak padi merupakan sumber nutrisi yang bernilai ekonomi tinggi yang dapat dimanfaatkan sebagai media pertumbuhan jamur tiram putih (Pleurotus ostreatus). Tujuan penelitian ini adalah (1) mengetahui pertumbuhan miselium jamur tiram putih (P. ostreatus) pada substrat limbah tandan kosong kelapa sawit dan dedak padi; (2) mengetahui karakteristik pertumbuhan jamur tiram putih (P. ostreatus) pada perlakuan jenis substrat yang berbeda. Penelitian ini telah di lakukan dalam rancangan acak lengkap (RAL) yang terdiri tujuh perlakuan dan tiga kali pengulangan. Susunan perlakuan merupakan perbandingan antara tandan kosong kelapa sawit : dedak padi, yakni P1 (50% : 50%), P2 (45% : 55%), P3 (40% : 60%), P4 (35% : 65%). P5 (30% : 70%), P6 (25% : 75%), P7 (serbuk geragaji 70%, dedak padi 20%, tepung jagung 10%). Adapun hasil penelitian menunjukkan laju pertumbuhan miselium tercepat pada perlakuan P1 yaitu 89 mm dan terendah pada perlakuan P5 yaitu 80 mm. Jumlah koloni tertinggi pada perlakuan P5 yaitu 1,4x1012 CFU/g dan terendah pada perlakuan P4 yaitu 0,4x1012 CFU/g. Waktu inkubasi paling cepat pada perlakuan P4 yaitu 28 hari dan terendah pada perlakuan P7 yaitu 33 hari.

EFEKTIVITAS PENGGUNAAN JARING KABUT DALAM KOLEKSI CHIROPTERA (MEGACHIROPTERA DAN MICROCHIROPTERA)

The use of traps is very important in the process of sampling bats in the field. The mist net is a type of traps that commonly used to catch bats for research purposes. This study aims to determine the effectiveness of using mist nets in bats sampling. Sampling site located in the Lindu Plain, District of Sigi, Central Sulawesi. Bats were sampled by mist nets using purposive sampling method, at four different types of habitat. The result of this study indicated that mist net is effective in catching fruit-eating bats (Megachiroptera), but is not effective in capturing insectivorous bats (Microchiroptera).

EKSPLORASI Actinomycetes SEBAGAI KANDIDAT ANTIBAKTERI PATOGEN YANG RESISTEN DARI RHIZOSFER TUMBUHAN LEDA (Eucalyptus deglupta Blume.) DI TAMAN NASIONAL LORE LINDU, INDONESIA

Actinomycetes that live in the rhizosphere of the Leda plant (Eucalyptus deglupta Blume.) Have the potential to produce pathogenic antibacterial compounds in humans. The ecosystems thought to be inhabited by Actinomycetes that produce pathogenic antibacterial compounds in humans is the rhizosphere of Leda endemic plant in the Lore Lindu National Park, Central Sulawesi. The purpose of this study was to isolate various isolates of Leda plant rhizosphere Actinomycetes in TNLL as a producer of pathogenic antibacterial compounds in humans. This research is divided into several stages. The first stage was sampling, isolation, and screening of Actinomycetes producing human pathogenic antibacterial compounds. Rhizosphere soil samples were taken by purposive sampling method combined with the quadrant method by measuring various environmental factors. The second stage is a selection to analyze the potential and efficacy of Actinomycetes isolates as producing antibacterial compounds against pathogenic bacteria in humans. The results showed that the condition of Leda plant vegetation in the Anaso-Rorekatimbu area was generally the same for each spot and supported the presence of Actinomycetes. The density of Actinomycetes is relatively the same for each spot. As many as 15 isolates were isolated from the Leda rhizosphere of Actinomycetes. Based on spore morphological and biochemical characterization, the 15 isolates were included in the Genus Streptomyces. Actinomycetes isolates L213, L433, and L411 were the most potent isolates and had broad-spectrum inhibition, because they were able to inhibit the four test bacteria S. aureus, MRSA, V. cholera, and EPEC. Keywords: Actinomycetes, Eucalyptus deglupta Blume., and pathogenic antibacterial