Fluorescence lifetime of indocyanine green in molecular and nano-forms in the cellular model of a brain tumor in vitro

Abstract Any surgical intervention to a central nervous system requires special accuracy and selectivity of the effect on the cancer cells. The application of laser-spectroscopic methods provides a unique opportunity to non-invasively determine the most significant parameters that characterize the tissue states. Moreover, non-invasive state assessment of the brain tumor tissue and surrounding tissues is essential for performing a relapse-free operation. Indocyanine Green (ICG) is a photosensitizer approved for clinical practice and has absorption peak in the near-infrared range corresponding to the spectral transparency window of biological tissue. Also, the aqueous colloidal solution of ICG aggregates was used for spectroscopic properties research in glioma tumor cells. The comparative analysis of ICG in molecular and nanoforms demonstrate the difference between spectral values which allow distinguishing monomers and aggregates in tumor tissue.

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Non-invasive, real-time reporting drug release in vitro and in vivo

Chemical Communications ◽

10.1039/c4cc09920f ◽

2015 ◽

Vol 51 (32) ◽

pp. 6948-6951 ◽

Cited By ~ 31

Author(s):

Yanfeng Zhang ◽

Qian Yin ◽

Jonathan Yen ◽

Joanne Li ◽

Hanze Ying ◽

...

Keyword(s):

Drug Release ◽

Real Time ◽

Near Infrared ◽

Reporting System ◽

Real Time Monitoring ◽

Near Infrared Fluorescence ◽

Non Invasive ◽

Fluorescence Dye

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

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Influence of Hyperthermia on Efficacy and Uptake of Carbon Nanohorn-Cisplatin Conjugates

Journal of Biomechanical Engineering ◽

10.1115/1.4026318 ◽

2014 ◽

Vol 136 (2) ◽

Cited By ~ 14

Author(s):

Matthew R. DeWitt ◽

Allison M. Pekkanen ◽

John Robertson ◽

Christopher G. Rylander ◽

Marissa Nichole Rylander

Keyword(s):

Cellular Uptake ◽

Near Infrared ◽

Drug Efficacy ◽

Infrared Light ◽

Exterior Surface ◽

Mild Hyperthermia ◽

Carbon Nanohorns ◽

Thermal Enhancement ◽

The Difference

Single-walled carbon nanohorns (SWNHs) have significant potential for use in photothermal therapies due to their capability to absorb near infrared light and deposit heat. Additionally, their extensive relative surface area and volume makes them ideal drug delivery vehicles. Novel multimodal treatments are envisioned in which laser excitation can be utilized in combination with chemotherapeutic-SWNH conjugates to thermally enhance the therapeutic efficacy of the transported drug. Although mild hyperthermia (41–43 °C) has been shown to increase cellular uptake of drugs such as cisplatin (CDDP) leading to thermal enhancement, studies on the effects of hyperthermia on cisplatin loaded nanoparticles are currently limited. After using a carbodiimide chemical reaction to attach CDDP to the exterior surface of SWNHs and nitric acid to incorporate CDDP in the interior volume, we determined the effects of mild hyperthermia on the efficacy of the CDDP-SWNH conjugates. Rat bladder transitional carcinoma cells were exposed to free CDDP or one of two CDDP-SWNH conjugates in vitro at 37 °C and 42 °C with the half maximal inhibitory concentration (IC50) for each treatment. The in vitro results demonstrate that unlike free CDDP, CDDP-SWNH conjugates do not exhibit thermal enhancement at 42 °C. An increase in viability of 16% and 7% was measured when cells were exposed at 42 deg compared to 37 deg for the surface attached and volume loaded CDDP-SWNH conjugates, respectively. Flow cytometry and confocal microscopy showed a decreased uptake of CDDP-SWNH conjugates at 42 °C compared to 37 °C, revealing the importance of nanoparticle uptake on the CDDP-SWNH conjugate's efficacy, particularly when hyperthermia is used as an adjuvant, and demonstrates the effect of particle size on uptake during mild hyperthermia. The uptake and drug release studies elucidated the difference in viability seen in the drug efficacy studies at different temperatures. We speculate that the disparity in thermal enhancement efficacy observed for free drug compared to the drug SWNH conjugates is due to their intrinsic size differences and, therefore, their mode of cellular uptake: diffusion or endocytosis. These experiments indicate the importance of tuning properties of nanoparticle-drug conjugates to maximize cellular uptake to ensure thermal enhancement in nanoparticle mediated photothermal-chemotherapy treatments.

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Indocyanine Green-Nexturastat A-PLGA Nanoparticles Combine Photothermal and Epigenetic Therapy for Melanoma

Nanomaterials ◽

10.3390/nano10010161 ◽

2020 ◽

Vol 10 (1) ◽

pp. 161 ◽

Cited By ~ 4

Author(s):

Debbie K. Ledezma ◽

Preethi B. Balakrishnan ◽

Juliana Cano-Mejia ◽

Elizabeth E. Sweeney ◽

Melissa Hadley ◽

...

Keyword(s):

Indocyanine Green ◽

Near Infrared ◽

Specific Activity ◽

Melanoma Cells ◽

Plga Nanoparticles ◽

Epigenetic Therapy ◽

Photothermal Heating ◽

Epigenetic Drug

In this study, we describe poly (lactic-co-glycolic) acid (PLGA)-based nanoparticles that combine photothermal therapy (PTT) with epigenetic therapy for melanoma. Specifically, we co-encapsulated indocyanine green (ICG), a PTT agent, and Nexturastat A (NextA), an epigenetic drug within PLGA nanoparticles (ICG-NextA-PLGA; INAPs). We hypothesized that combining PTT with epigenetic therapy elicits favorable cytotoxic and immunomodulatory responses that result in improved survival in melanoma-bearing mice. We utilized a nanoemulsion synthesis scheme to co-encapsulate ICG and NextA within stable and monodispersed INAPs. The INAPs exhibited concentration-dependent and near-infrared (NIR) laser power-dependent photothermal heating characteristics, and functioned as effective single-use agents for PTT of melanoma cells in vitro. The INAPs functioned as effective epigenetic therapy agents by inhibiting the expression of pan-histone deacetylase (HDAC) and HDAC6-specific activity in melanoma cells in vitro. When used for both PTT and epigenetic therapy in vitro, the INAPs increased the expression of co-stimulatory molecules and major histocompatibility complex (MHC) Class I in melanoma cells relative to controls. These advantages persisted in vivo in a syngeneic murine model of melanoma, where the combination therapy slowed tumor progression and improved median survival. These findings demonstrate the potential of INAPs as agents of PTT and epigenetic therapy for melanoma.

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Tungsten disulfide-based nanocomposites for photothermal therapy

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.10.81 ◽

2019 ◽

Vol 10 ◽

pp. 811-822 ◽

Cited By ~ 3

Author(s):

Tzuriel Levin ◽

Hagit Sade ◽

Rina Ben-Shabbat Binyamini ◽

Maayan Pour ◽

Iftach Nachman ◽

...

Keyword(s):

Photothermal Therapy ◽

Near Infrared ◽

Transition Metal Dichalcogenides ◽

Tungsten Disulfide ◽

Ceric Ammonium Nitrate ◽

Infrared Range ◽

Metal Dichalcogenides ◽

Current Article ◽

Functionalized Nanotubes

Nanostructures of transition-metal dichalcogenides (TMDC) have raised scientific interest in the last few decades. Tungsten disulfide (WS2) nanotubes and nanoparticles are among the most extensively studied members in this group, and are used for, e.g., polymer reinforcement, lubrication and electronic devices. Their biocompatibility and low toxicity make them suitable for medical and biological applications. One potential application is photothermal therapy (PTT), a method for the targeted treatment of cancer, in which a light-responsive material is irradiated with a laser in the near-infrared range. In the current article we present WS2 nanotubes functionalized with previously reported ceric ammonium nitrate–maghemite (CAN-mag) nanoparticles, used for PTT. Functionalization of the nanotubes with CAN-mag nanoparticles resulted in a magnetic nanocomposite. When tested in vitro with two types of cancer cells, the functionalized nanotubes showed a better PTT activity compared to non-functionalized nanotubes, as well as reduced aggregation and the ability to add a second-step functionality. This ability is demonstrated here with two polymers grafted onto the nanocomposite surface, and other functionalities could be additional cancer therapy agents for achieving increased therapeutic activity.

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Improved Speciation Characteristics of PEGylated Indocyanine Green-Labeled Panitumumab: Revisiting the Solution and Spectroscopic Properties of a Near-Infrared Emitting anti-HER1 Antibody for Optical Imaging of Cancer

Bioconjugate Chemistry ◽

10.1021/bc100336b ◽

2010 ◽

Vol 21 (12) ◽

pp. 2305-2312 ◽

Cited By ~ 20

Author(s):

Aaron Joseph L. Villaraza ◽

Diane E. Milenic ◽

Martin W. Brechbiel

Keyword(s):

Indocyanine Green ◽

Optical Imaging ◽

Near Infrared ◽

Spectroscopic Properties

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Potential of solubility, enzymatic methods and NIRS to predict in situ rumen escape protein

Netherlands Journal of Agricultural Science ◽

10.18174/njas.v45i2.519 ◽

1997 ◽

Vol 45 (2) ◽

pp. 291-306 ◽

Cited By ~ 3

Author(s):

J.L. De Boever ◽

B.G. Cottyn ◽

J.M. Vanacker ◽

C.V. Boucque

Keyword(s):

Phosphate Buffer ◽

Near Infrared ◽

Streptomyces Griseus ◽

Maize Silage ◽

Near Infrared Reflectance ◽

In Vitro Method ◽

Rumen Degradation ◽

The Difference

The percentage of feed protein escaping rumen degradation was measured by the in situ method (%EPsitu) for 29 compound feeds, untreated and formaldehyde-treated soyabean meal and 12 forages: 3 grass silages, 2 maize silages, fresh grass, grass hay, fodder beets, fresh potatoes, ensiled beet pulp, chopped ear-maize silage and brewers' grains. Loss of particles through bag pores was determined by the difference between the washable fraction (W) and the fraction soluble in borate-phosphate buffer at pH 6.7 (S). W - S was most pronounced for compound feeds (on average 14.4 percentage units), for brewers' grains and maize silages. A correction of %EPsitu, assuming that W - S degrades like the potentially degradable fraction, was not appropriate. Solubility in borate-phosphate buffer after 1 h, enzymic degradability by protease from Streptomyces griseus or ficin after 1, 6 and 24 h and near infrared reflectance spectroscopy (NIRS) (for compound feeds alone) were examined as a routine method to predict %EPsitu. With the buffer and S. griseus the effect of pH (6.7 vs. 8.0) and at pH 8.0 the effect of amount of substrate (500-mg sample vs. 20 mg N) were tested. With ficin, 500-mg samples were incubated at pH 6.7. Predictions were better when compound feeds and forages were considered separately. However, the best in vitro method was different for the 2 feed categories, being solubility in buffer for the compound feeds and enzymic degradation of a constant amount of protein with S. griseus at pH 8.0 for forages. NIRS showed potential to predict %EPsitu of compound feeds, but needs more reference samples. The Dutch feed tables appeared more accurate than the best in vitro method for compound feeds, but was too inaccurate for some forages like fodder beets, maize silage and ear-maize silage.

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Near infrared spectroscopy for non-invasive assessment of intracranial haemoglobin oxygenation in an in vitro model of the calf head

Research in Veterinary Science ◽

10.1016/s0034-5288(98)90159-x ◽

1998 ◽

Vol 65 (2) ◽

pp. 103-109 ◽

Cited By ~ 8

Author(s):

John Pringle ◽

Tatiana Art ◽

Pierre Lekeux

Keyword(s):

Infrared Spectroscopy ◽

Near Infrared Spectroscopy ◽

In Vitro Model ◽

Near Infrared ◽

Non Invasive ◽

Vitro Model

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Second near-infrared (NIR-II) imaging: a novel diagnostic technique for brain diseases

Reviews in the Neurosciences ◽

10.1515/revneuro-2021-0088 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Na Xie ◽

Ya Hou ◽

Shaohui Wang ◽

Xiaopeng Ai ◽

Jinrong Bai ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Tumor ◽

Near Infrared ◽

Diagnostic Technique ◽

High Sensitivity ◽

Brain Diseases ◽

Tissue Penetration ◽

Tumor Inflammation

Abstract Imaging in the second near-infrared II (NIR-II) window, a kind of biomedical imaging technology with characteristics of high sensitivity, high resolution, and real-time imaging, is commonly used in the diagnosis of brain diseases. Compared with the conventional visible light (400–750 nm) and NIR-I (750–900 nm) imaging, the NIR-II has a longer wavelength of 1000–1700 nm. Notably, the superiorities of NIR-II can minimize the light scattering and autofluorescence of biological tissue with the depth of brain tissue penetration up to 7.4 mm. Herein, we summarized the main principles of NIR-II in animal models of traumatic brain injury, cerebrovascular visualization, brain tumor, inflammation, and stroke. Simultaneously, we encapsulated the in vivo process of NIR-II probes and their in vivo and in vitro toxic effects. We further dissected its limitations and following optimization measures.

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