Xylanase Production via Aspergillus niger: Effect of Carbon Source and Composition
Abstract Xylanases are secondary metabolite product of variety organisms from various agriculture wastes. Xylanases’ demands in industrial level are increasing. Precisely, the necessity of enzyme such as xylanase to breaks down the xylan into reducing sugar for biofuel production is inevitable. However, the production of xylanases is insufficient to support the market demand. Hence, Aspergillus niger is used as the xylanase producer in this research. In this study, effect of carbon sources (corn cob-based xylan (CCX) and empty fruit bunches (EFB)) and concentrations of carbon source (2.5g/L to 4.0g/L) on xylanase production through One-factor-at-time (OFAT) experimental technique were investigated. The optimum fermentation period of 5 days determined by using mycelial dry cell mass and Bradford protein concentration growth profile is 5 days was set as the incubation period. Among these data, both carbon sources shown the maximum value at concentration of 3.5g/L. CCX showed a higher xylanase concentration (0.882±0.005μg/mL) compared to EFB (0.533 ± 0.006μg/mL). Hence, among these data analysis CCX has a better performance compared to EFB. Hence, among these data analysis CCX has a better performance compared to EFB.