Inactivation of SARS-CoV-2 in clinical exhaled breath condensate samples for metabolomic analysis

2021 ◽  
Author(s):  
Shuang Hu ◽  
Mitchell M McCartney ◽  
Juan Arredondo ◽  
Sumathi Sankaran-Walters ◽  
Eva Borras ◽  
...  
Keyword(s):  
Clinical Setting ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Metabolomic Analysis ◽  
Health And Disease ◽  
Consistent Method ◽  
First Time ◽  
Breath Condensate

Abstract Exhaled breath condensate (EBC) is routinely collected and analyzed in breath research. Because it contains aerosol droplets, EBC samples from SARS-CoV-2 infected individuals harbor the virus and pose the threat of infectious exposure. We report for the first time a safe and consistent method to fully inactivate SARS-CoV-2 in EBC samples and make EBC samples safe for processing and analysis. EBC samples containing infectious SARS-CoV-2 were treated with several concentrations of acetonitrile. The most commonly used 10% acetonitrile treatment for EBC processing failed to completely inactivate the virus in samples and viable virus was detected by the assay of SARS-CoV-2 infection of Vero E6 cells in a BSL-3 laboratory. Treatment with either 50% or 90% acetonitrile was effective to completely inactivate the virus, resulting in safe, non-infectious EBC samples that can be used for metabolomic analysis. Our study provides SARS-CoV-2 inactivation protocol for the collection and processing of EBC samples in the clinical setting and for advancing to metabolic assessments in health and disease.

NMR Metabolomic Analysis of Exhaled Breath Condensate of Asthmatic Patients at Two Different Temperatures

2014 ◽  
Vol 13 (12) ◽  
pp. 6107-6120 ◽  
Author(s):  
Andrea Motta ◽  
Debora Paris ◽  
Maria D’Amato ◽  
Dominique Melck ◽  
Cecilia Calabrese ◽  
...  
Keyword(s):  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Metabolomic Analysis ◽  
Asthmatic Patients ◽  
Different Temperatures ◽  
Breath Condensate

scholarly journals Is NMR-based metabolomic analysis of exhaled breath condensate accurate?

2011 ◽  
Vol 37 (2) ◽  
pp. 468-470 ◽  
Author(s):  
J. L. Izquierdo-Garcia ◽  
G. Peces-Barba ◽  
S. Heili ◽  
R. Diaz ◽  
E. Want ◽  
...  
Keyword(s):  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Metabolomic Analysis ◽  
Breath Condensate

Preliminary Analysis of the Proteome of Exhaled Breath Condensate in Bottlenose Dolphins (Tursiops truncatus)

2018 ◽  
Vol 44 (3) ◽  
pp. 256-266 ◽  
Author(s):  
Don R. Bergfelt ◽  
John Lippolis ◽  
Michel Vandenplas ◽  
Sydney Davis ◽  
Blake A. Miller ◽  
...  
Keyword(s):  
Preliminary Analysis ◽  
Tursiops Truncatus ◽  
Exhaled Breath Condensate ◽  
Bottlenose Dolphins ◽  
Exhaled Breath ◽  
Breath Condensate

Simultaneous and Sensitive Determination of Amphetamine, Codeine and Morphine in Exhaled Breath Condensate, Using Capillary Electrophoresis Coupled with On-line and Off-line Enhancing Methods

2020 ◽  
Vol 16 (7) ◽  
pp. 872-879
Author(s):  
Samin Hamidi
Keyword(s):  
Drugs Of Abuse ◽  
Exhaled Breath Condensate ◽  
Direct Injection ◽  
Forensic Toxicology ◽  
Analytical Signal ◽  
Exhaled Breath ◽  
Concentration Method ◽  
Validation Parameters ◽  
Breath Condensate

Background: Abuse of drugs is associated with several medical, forensic, toxicology and social challenges. “Drugs of abuse” testing is therefore an important issue. Objective: We propose a simple CE-based method for the quantification of amphetamine, codeine and morphine after direct injection of Exhaled Breath Condensate (EBC) by the aid of simple stacking mode and an off-line pre-concentration method. Methods: Using graphene oxide adsorbents, amphetamine, codeine and morphine were extracted from EBC in order to eliminate the proteins and other interferences. In addition to off-line method, an online stacking mode was applied to improve the analytical signal obtained from the instrument. Results: The validation parameters were experimented on the developed method based on the FDA guideline over concentration ranges of 12.5-100, 30-500 and 10-1250 ng/mL associated with amphetamine, codeine and morphine, respectively. Small volumes (around 100 μL) of EBC were collected using a lab-made setup and successfully analyzed using the proposed method where precisions and accuracies (within day and between days) were in accordance with the guideline (recommended less than 15 % for biological samples). The recovery tests were used to evaluate the matrix effect and data (94 to 105 %) showed that the proposed method can be applied in different EBC matrix samplings of subjects. Conclusion: The proposed method is superior for simultaneous determination of amphetamine, codeine and morphine over chromatographic analyses because it is fast and consumes fewer chemicals, with no derivatization step.

Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate

2020 ◽  
Vol 16 (8) ◽  
pp. 1032-1040
Author(s):  
Laleh Samini ◽  
Maryam Khoubnasabjafari ◽  
Mohamad M. Alimorad ◽  
Vahid Jouyban-Gharamaleki ◽  
Hak-Kim Chan ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Exhaled Breath Condensate ◽  
Low Cost ◽  
Exhaled Breath ◽  
Chromatography Method ◽  
Extraction Solvent ◽  
Drug Concentrations ◽  
Dispersive Liquid Liquid Microextraction ◽  
Breath Condensate

Background: Analysis of drug concentrations in biological fluids is required in clinical sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is a potential sample for follow up of drug concentrations. Methods: A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated liquid chromatography method was employed for the determination of budesonide (BDS) in EBC samples collected using a homemade setup. EBC is a non-invasive biological sample with possible applications for monitoring drug concentrations. The proposed analytical method is validated according to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected from healthy volunteers receiving a single puff of BDS. Results: The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent, chloroform (200 μL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The method was validated over a concentration range of 21-210 μg·L-1 in EBC. Inter- and intra-day precisions were less than 10% where the acceptable levels are less than 20%. The validated method was successfully applied for the determination of BDS in EBC samples. Conclusion: The findings of this study indicate that the developed method can be used for the extraction and quantification of BDS in EBC samples using a low cost method.

Determination of Verapamil in Exhaled Breath Condensate by Using Microextraction and Liquid Chromatography

2019 ◽  
Vol 15 (5) ◽  
pp. 535-541 ◽  
Author(s):  
Fariba Pourkarim ◽  
Ali Shayanfar ◽  
Maryam Khoubnasabjafari ◽  
Fariborz Akbarzadeh ◽  
Sanaz Sajedi-Amin ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Good Precision ◽  
Dispersive Liquid Liquid Microextraction ◽  
Breath Condensate ◽  
Liquid Microextraction

Background:Developing a simple analysis method for quantification of drug concentration is one of the essential issues in pharmacokinetic and therapeutic drug monitoring studies.Objective:A fast and reliable dispersive liquid-liquid microextraction procedure was employed for preconcentration of verapamil in exhaled breath condensate (EBC) samples and this was followed by the determination with high-performance liquid chromatography-ultraviolet detection.Methods:A reverse-phase high-performance liquid chromatography (RP-HPLC) combined with a dispersive liquid-liquid microextraction method (DLLME) was applied for quantification of verapamil in the EBC samples. The developed method was validated according to FDA guidelines.Results:Under the optimum conditions, the method provided a linear range between 0.07 and 0.8 µg.mL-1 with a coefficient of determination of 0.998. The intra- and inter-day relative standard deviation and relative error values of the method were below 15%, which indicated good precision and accuracy. The proposed method was successfully applied for the analysis of verapamil in two real samples with concentrations of 0.07 and 0.09 µg.mL-1.Conclusion:The established HPLC-UV-DLLME method could be applied for the analysis of verapamil in human EBC samples.

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