scholarly journals Antibacterial activity roll on deodorant with Pluchea indica (L.) leaf extract against Staphylococcus epidermidis (Evans 1916 ) in vitro

2019 ◽  
Vol 293 ◽  
pp. 012031
Author(s):  
O Komala ◽  
I Y Wiendarlina ◽  
N Rizqiyana
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract

scholarly journals In vitro Study on Anti-salmonella Activities of Boerhaavia diffusa (L. syn) Leaf Extract

2019 ◽  
pp. 1-10
Author(s):  
Ologun, Oluwabusayo ◽  
Dada, Ebenezer Oluyemi ◽  
Ajayi, Kehinde Oluyemi
Keyword(s):  
Antibacterial Activity ◽  
Clinical Isolate ◽  
Optical Density ◽  
Aqueous Extract ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Boerhaavia Diffusa ◽  
Broth Dilution

Various strategies have been employed in the treatment and management of Salmonella infection however, Salmonella strains have gained resistance to antibiotics. This study was to determine in vitro anti-Salmonella activity of Boerhaavia diffusa leaf extract against clinical isolate of Salmonella typhi and Salmonella typhi ATCC 14028. The aqueous and ethanol extracts of B. diffusa were studied for their antibacterial activity against pathogenic Salmonella typhi. This study was carried out between April and September 2018. The in vitro antibacterial activity was performed by agar well diffusion method and broth dilution using spectrophotometric method and the results were expressed as the average diameter of zone of inhibition of bacterial growth around the well and optical density respectively. It was observed that aqueous extract exerted slightly higher activity than ethanolic extract as revealed by the mean diameter of zone of inhibitions at a concentration of 200 mg/ml, the aqueous extract had 35.21±0.47 mm (Salmonella typhi ATCC 14028) compared with ethanol extract 26.41±0.32 mm (clinical). However, in the broth dilution method, ethanol extract significantly (p=0.05) reduced the cell, at 48 hours, the optical density of clinical isolate of S. typhi treated at concentration of 200 mg/ml of extract was 0.47±0.02 nm while at the same concentration of extract, aqueous extract had an optical density of 0.52±0.11 nm respectively. The phytochemical assay revealed that tannin (5.18±0.02 mg/g) and quinone (8.45±0.13 mg/g) in ethanol extract was significantly (p=0.05) higher than aqueous extract while saponin (14.18±0.06 mg/g) was higher in the aqueous extract. The ethanol and aqueous extracts of leaves of B. diffusa whole plant exhibited significant antibacterial activity against both clinical and typed Salmonella typhi. Therefore, the plant extract could be used for the treatment of Salmonellosis, however, the in vivo studies is needed to ascertain the safety of the extract.

scholarly journals Microbiological Assay for Ceftazidime Injection

2007 ◽  
Vol 90 (5) ◽  
pp. 1379-1382 ◽  
Author(s):  
Andréia De Haro Moreno ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Antibacterial Activity ◽  
Regression Analysis ◽  
Standard Deviation ◽  
Staphylococcus Epidermidis ◽  
Test Organism ◽  
Calibration Graph ◽  
Microbiological Assay ◽  
Parallel Model ◽  
Relative Standard

Abstract A simple, sensitive, and specific biodiffusion assay for the antibacterial ceftazidime was developed using a strain of Staphylococcus epidermidis (ATCC 12228) as the test organism. Ceftazidime was measured in powder for injection at concentrations ranging from 100 to 400 g/mL. The calibration graph for ceftazidime was linear (r2 = 1), and the method validation showed that it was precise (relative standard deviation = 0.415) and accurate. The results obtained by biodiffusion assay were statistically calculated by linear parallel model and by means of regression analysis and were verified using analysis of variance. It was concluded that the microbiological assay is satisfactory for in vitro quantification of the antibacterial activity of ceftazidime in pharmaceuticals.

scholarly journals Increased Killing of Staphylococci and Streptococci by Daptomycin Compared with Cefazolin and Vancomycin in an In Vitro Peritoneal Dialysate Model

2003 ◽  
Vol 47 (12) ◽  
pp. 3764-3767 ◽  
Author(s):  
Elizabeth D. Hermsen ◽  
Laurie B. Hovde ◽  
John R. Hotchkiss ◽  
John C. Rotschafer
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Bacteriostatic Effect ◽  
Peritoneal Dialysate ◽  
Streptococcus Sanguis ◽  
Promising Agent ◽  
Mueller Hinton ◽  
Dependent Activity ◽  
Mueller Hinton Broth

ABSTRACT Peritoneal dialysate fluid (PDF) is a bacteriostatic medium that compromises the antibacterial activity of cell wall-active agents. By use of an in vitro static model, methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus (MSSA), methicillin-susceptible Staphylococcus epidermidis (MSSE), and Streptococcus sanguis were exposed to daptomycin at concentrations of 10, 30, and 100 mg/liter, cefazolin at 125 mg/liter, and vancomycin at 25 mg/liter in cation-adjusted Mueller-Hinton Broth or Todd Hewitt Broth (for S. sanguis) and PDF at pHs of 5.5 and 7.4. The pH had no effect on antibacterial activity. Neither cefazolin nor vancomycin produced a bactericidal or a bacteriostatic effect versus MRSA, MSSA, MSSE, or S. sanguis in PDF, while all concentrations of daptomycin were bactericidal against all organisms in PDF. Daptomycin did not exhibit concentration-dependent activity in PDF. Daptomycin appears to be a promising agent for use in peritoneal dialysis-associated peritonitis, producing bacterial kill to a greater extent and at a higher rate than cefazolin or vancomycin in PDF.

scholarly journals Anti Acne Vulgaris Topical Cream From Combination Extract of Gymnanthemum Amygdalinum Del. and Elaeis Guineensis Jacq. Leaves

2020 ◽  
Vol 3 (1) ◽  
pp. 42-50
Author(s):  
Wahyudi ◽  
Ayu Syahfitri ◽  
Syukur Berkat Waruwu ◽  
Dian Ika Perbina
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Acne Vulgaris ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Propionibacterium Acne ◽  
Self Confidence ◽  
Adolescents And Adults

Acne is one problem in adolescents and adults who can reduce self-confidence. This study aimed to formulate the combination cream of African leaf extract and palm oil leaves to determine the antibacterial activity of African leaf extract against several acne-causing bacteria (Propionibacterium acne and Staphylococcus epidermidis). Test antibacterial activity of extracts and cream with disc diffusion method. The cream is made with concentration of 5, 10 and 20% w/w. The evaluation results of cream with concentration of 5, 10 and 20% were physically stable for 4 weeks of storage at temperature room, homogeneous, pH and it did not cause irritation. Based on the test results, a combination of African leaf extract with concentration of 5% effectively inhibited bacterial growth with an average inhibition zone diameter of 12.06 mm for Propionibacterium acne bacteria and 13.63 mm for Staphylococcus epidermidis bacteria. The result of this study suggest that combination of African leaf extract and palm leaves posses potent anti-bacterial activity against Propionibacterium acne and Staphylococcus epidermidis.

scholarly journals Aktivitas Antibakteri Ekstrak Daun Jarak Pagar (Jatropha Curcas L) Terhadap Staphylococcus Epidermidis Dan Staphylococcus Aureus Secara In Vitro

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Susilo Yulianto ◽  
Sunarmi Sunarmi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Jatropha Curcas ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibitory Zone ◽  
Zone Diameter ◽  
Jatropha Curcas L ◽  
Inhibitory Power ◽  
And Staphylococcus Aureus

Abstract: Jatropha Leaves, Staphylococcus Epidermidis, Staphylococcus Aureus. Jatropha curcas L leaves contain tannins, saponins and flavonoids. According to some studies, tannins, saponins and flavonoids have antibacterial power. Based on these indications, it is important to do research on antibacterial potency of jatropha leaf extract (Jatropha curcas L) against Staphylococcus epidermidis and Staphylococcus aureus bacteria. The aim of this research is to know Antibacterial Activity of Jatropha curcas L Extract on Staphylococcus epidermidis and Staphylococcus aureus". This research. An experimental study by testing the antibacterial activity of leaf extract and jatropha result of maceration, infundation and reflux as measured by Minimal Inhibitory Concentration (KHM) on growth of Staphylococcus epidermidis and Staphylococcus aureus bacteria. From the results of the research, it was found that the extract of the reflux was brownish green and the characteristic of jatropha leaves. The amount of extract produced was 17.03 g, so the yield was 17.03%. Jatropha leaf extract of maceration has no inhibitory power to S. epidermidis in 4 series concentrations starting from 3.125 ppm to 25.000 ppm. Inhibitory zones begin to appear at concentrations of 50,000 ppm and increase at concentrations of 100,000 ppm. The drag zone diameter in the Jatropha curcas extract of the reflux showed the inhibitory power in all series of concentrations. The smallest inhibitory zone was shown at a concentration of 3,125 ppm (9.33 mm) and increased with increasing concentrations. The diameter of the inhibit zone was greatest at 100,000 ppm concentrations of 22.67 mm. Description of inhibitory zone diameter of methanol leaf extract of jatropha result of maceration and reflux to S. epidermidis. The conclusion of this research is extract of reflux result giving bigger resistance than maceration extract to S. epidermidis and S. aureus. Methanol extract of jatropha from the maceration and reflux have strong antibacterial activity against S. epidermidis and S. aureus but still under Klindamycin.

scholarly journals In vitro cytotoxicity and antibiotic application of green route surface modified ferromagnetic TiO2 nanoparticles

RSC Advances ◽  
2019 ◽  
Vol 9 (23) ◽  
pp. 13254-13262 ◽  
Author(s):  
A. K. M. Atique Ullah ◽  
A. N. Tamanna ◽  
A. Hossain ◽  
M. Akter ◽  
M. F. Kabir ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Tio2 Nanoparticles ◽  
Leaf Extract ◽  
In Vitro Cytotoxicity ◽  
Artocarpus Heterophyllus ◽  
High Antibacterial Activity ◽  
Green Route ◽  
Surface Modified

Artocarpus heterophyllus leaf extract mediated green synthesized TiO2 nanoparticles exhibit less toxicity with high antibacterial activity.

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