scholarly journals Percentage Value of Membrane Integrity and Acrosome Integrity Spermatozoa in Simmental Liquid Semen with Addition Penicillin and Sweet Orange Essential Oil

2019 ◽  
Vol 327 ◽  
pp. 012027
Author(s):  
S A Sitepu ◽  
J Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Acrosome Integrity ◽  
Orange Essential Oil

scholarly journals COMBINED EFFECT OF STREPTOMYCIN AND SWEET ORANGE ESSENTIAL OIL TO MEMBRANE AND ACROSOME INTEGRITY BOER GOAT FROZEN SEMEN

2019 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Boer Goat ◽  
Randomized Design ◽  
Frozen Semen ◽  
Acrosome Integrity ◽  
Completely Randomized Design ◽  
Orange Essential Oil

One of factors that cause a bad quality of Boer Goat frozen semen is the growth of bacterial. This can be overcome by adding antibiotics such as streptomycin. To further suppress the growth of bacteria can be added other ingredients that contain antibacterials such as sweet orange essential oil. The purpose of this research is to know the percentage value of Membrane Integrity and Acrosome Integrity on Boer Goat frozen semen with addition sweet orange essential oil and streptomycin. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment in this research is addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and streptomycin extender. The results showed the best treatment addition combination streptomycin and sweet orange essential oil to percentage Membrane Integrity and Acrosome Integrity is increase 1% sweet orange essential oil.Keywords: Boer Goat, essential oil, frozen semen, streptomycin, sweet orange.

scholarly journals Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

2020 ◽  
pp. 42-46
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Integrity Test ◽  
Randomized Design ◽  
Simmental Cattle ◽  
Fresh Semen ◽  
Orange Essential Oil

Aims: This study aimed to determine the quality of Simmental Bull liquid semen through laboratory tests, including the percentage value of membrane integrity Spermatozoa with the addition of gentamycin and sweet orange essential oil to tris yolk extender. Methodology: The material used in this research is Simmental Cattle bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender was prepared with Tris (hydroxymethyl aminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquades (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replicates. The treatment given is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameter observed was the evaluation of membrane integrity before equilibration and after equilibration. Results: The results showed that the percentage of membrane integrity in Simmental bull liquid semen continues to increase with the addition of sweet orange essential oils. The results showed that the addition of sweet orange essential oil as much as 0% produced a membrane integrity percentage after equilibration of 63%, adding 0.25% resulted in 68%, adding 0.5% yielded 70%, adding 0.75% yielded 75% and adding 1% resulted in 79%. Conclusion: The best percentage of membrane integrity in Simmental bull liquid semen was the addition of gentamicin and sweet orange essential oil in the tris yolk extender at the level of 1%.

scholarly journals Acrosome Integrity Spermatozoa Test with Addition of Gentamicin and Sweet Orange Essential Oil in Simmental Bull Liquid Semen

2020 ◽  
pp. 1-5
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Citric Acid ◽  
Essential Oil ◽  
Sweet Orange ◽  
Egg Yolk ◽  
Randomized Design ◽  
Before And After ◽  
Acrosome Integrity ◽  
Fresh Semen ◽  
Orange Essential Oil

Aims: The purpose of this study was to determine the percentage value of spermatozoa acrosome integrity with the addition of gentamicin and sweet orange essential oil on the tris yolk extender on Simmental bull liquid semen. Methodology: The material that was used in this research included Simmental bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender prepares with Tris (hydroxymethyl) aminomethane (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aqua dest (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replications. The treatments comprised the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1% inclusion levels. The parameter observed was the evaluation of the percentage value of acrosome integrity before and after equilibration equilibration. Results: The results showed that the percentage value of acrosome integrity in spermatozoa linearly increased along with the addition of sweet orange essential oil. The addition of sweet orange essential oil had a very significant effect (P <0.01) on the percentage of spermatozoa acrosome integrity in Simmental bull liquid semen. The percentage of acrosome integrity on the addition of 0% (P0) sweet orange essential oil is 60%, the addition of 0.25% (P1) is 64%,the addition of 0.5% (P2) is 67%, the addition of 0.75% (P3) is 69%, and the addition of 1% (P4) is 73%. Conclusion: The addition of 1% (P4) sweet orange essential oil can increase the value of acrosome integrity on spermatozoa, which subsequently improve the quality of Simmental bull liquid semen.

Fumigant Activity of Sweet Orange Essential Oil Fractions Against Red Imported Fire Ants (Hymenoptera: Formicidae)

2017 ◽  
Vol 110 (4) ◽  
pp. 1556-1562 ◽  
Author(s):  
Wei Hu ◽  
Ning Zhang ◽  
Hongli Chen ◽  
Balian Zhong ◽  
Aixue Yang ◽  
...  
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Fire Ants ◽  
Fumigant Activity ◽  
Oil Fractions ◽  
Imported Fire Ants ◽  
Red Imported Fire Ants ◽  
Orange Essential Oil

Utility of Blended Polymeric Formulations Containing Cellulose Nanofibrils for Encapsulation and Controlled Release of Sweet Orange Essential Oil

2018 ◽  
Vol 11 (6) ◽  
pp. 1188-1198 ◽  
Author(s):  
Hugo Junior Barboza de Souza ◽  
Regiane Victória de Barros Fernandes ◽  
Soraia Vilela Borges ◽  
Pedro Henrique Campelo Felix ◽  
Lívia Cássia Viana ◽  
...  
Keyword(s):  
Controlled Release ◽  
Essential Oil ◽  
Sweet Orange ◽  
Cellulose Nanofibrils ◽  
Orange Essential Oil

scholarly journals Utilization Of Frozen Goat Semen With Addition Sweet Orange Essential Oil To Improve Genetic Quality in Ujung Teran Village

2019 ◽  
Vol 1 (2) ◽  
pp. 170-174
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Community Service ◽  
Artificial Insemination ◽  
Sweet Orange ◽  
Genetic Quality ◽  
Know How ◽  
Frozen Semen ◽  
And Training ◽  
Orange Essential Oil

One of the causes of underdeveloped goats is low genetic quality. The way that can be done to improve the genetic quality of goats is to do cross-breeding with superior goats through Artificial Insemination. The community service aims to increase the knowledge of goat farmers in Ujung Teran Village, Langkat Regency on how to make goat frozen semen using tris yolk extender plus sweet orange essential oil for Artificial Insemination program along with the benefits that can be obtained. The method of service is by giving lectures and training to goat farmers. The results obtained from the implementation of this program are that farmers know how to make frozen semen of goat using sweet orange essential oil on the tris yolk extender and are willing to make Artificial Insemination in their goat cattle.

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