scholarly journals Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

2020 ◽  
pp. 42-46
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Integrity Test ◽  
Randomized Design ◽  
Simmental Cattle ◽  
Fresh Semen ◽  
Orange Essential Oil

Aims: This study aimed to determine the quality of Simmental Bull liquid semen through laboratory tests, including the percentage value of membrane integrity Spermatozoa with the addition of gentamycin and sweet orange essential oil to tris yolk extender. Methodology: The material used in this research is Simmental Cattle bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender was prepared with Tris (hydroxymethyl aminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquades (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replicates. The treatment given is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameter observed was the evaluation of membrane integrity before equilibration and after equilibration. Results: The results showed that the percentage of membrane integrity in Simmental bull liquid semen continues to increase with the addition of sweet orange essential oils. The results showed that the addition of sweet orange essential oil as much as 0% produced a membrane integrity percentage after equilibration of 63%, adding 0.25% resulted in 68%, adding 0.5% yielded 70%, adding 0.75% yielded 75% and adding 1% resulted in 79%. Conclusion: The best percentage of membrane integrity in Simmental bull liquid semen was the addition of gentamicin and sweet orange essential oil in the tris yolk extender at the level of 1%.

scholarly journals Acrosome Integrity Spermatozoa Test with Addition of Gentamicin and Sweet Orange Essential Oil in Simmental Bull Liquid Semen

2020 ◽  
pp. 1-5
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Citric Acid ◽  
Essential Oil ◽  
Sweet Orange ◽  
Egg Yolk ◽  
Randomized Design ◽  
Before And After ◽  
Acrosome Integrity ◽  
Fresh Semen ◽  
Orange Essential Oil

Aims: The purpose of this study was to determine the percentage value of spermatozoa acrosome integrity with the addition of gentamicin and sweet orange essential oil on the tris yolk extender on Simmental bull liquid semen. Methodology: The material that was used in this research included Simmental bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender prepares with Tris (hydroxymethyl) aminomethane (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aqua dest (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replications. The treatments comprised the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1% inclusion levels. The parameter observed was the evaluation of the percentage value of acrosome integrity before and after equilibration equilibration. Results: The results showed that the percentage value of acrosome integrity in spermatozoa linearly increased along with the addition of sweet orange essential oil. The addition of sweet orange essential oil had a very significant effect (P <0.01) on the percentage of spermatozoa acrosome integrity in Simmental bull liquid semen. The percentage of acrosome integrity on the addition of 0% (P0) sweet orange essential oil is 60%, the addition of 0.25% (P1) is 64%,the addition of 0.5% (P2) is 67%, the addition of 0.75% (P3) is 69%, and the addition of 1% (P4) is 73%. Conclusion: The addition of 1% (P4) sweet orange essential oil can increase the value of acrosome integrity on spermatozoa, which subsequently improve the quality of Simmental bull liquid semen.

scholarly journals COMBINED EFFECT OF STREPTOMYCIN AND SWEET ORANGE ESSENTIAL OIL TO MEMBRANE AND ACROSOME INTEGRITY BOER GOAT FROZEN SEMEN

2019 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Boer Goat ◽  
Randomized Design ◽  
Frozen Semen ◽  
Acrosome Integrity ◽  
Completely Randomized Design ◽  
Orange Essential Oil

One of factors that cause a bad quality of Boer Goat frozen semen is the growth of bacterial. This can be overcome by adding antibiotics such as streptomycin. To further suppress the growth of bacteria can be added other ingredients that contain antibacterials such as sweet orange essential oil. The purpose of this research is to know the percentage value of Membrane Integrity and Acrosome Integrity on Boer Goat frozen semen with addition sweet orange essential oil and streptomycin. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment in this research is addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and streptomycin extender. The results showed the best treatment addition combination streptomycin and sweet orange essential oil to percentage Membrane Integrity and Acrosome Integrity is increase 1% sweet orange essential oil.Keywords: Boer Goat, essential oil, frozen semen, streptomycin, sweet orange.

scholarly journals Spermatozoa Abnormality Test of Boer Goats Frozen Semen with the Addition of Sweet Orange Essential Oil and Streptomycin in Tris Yolk Extender

2020 ◽  
pp. 20-23
Author(s):  
Sukma Aditya Sitepu
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Egg Yolk ◽  
Boer Goat ◽  
Average Value ◽  
Frozen Semen ◽  
Before And After ◽  
Boer Goats ◽  
Fresh Semen ◽  
Orange Essential Oil

This research aims to to determine the percentage value of spermatozoa abnormalities in Boer Goat frozen semen by adding a combination of streptomycin with sweet orange essential oil to tris yolk extender. The ingredients used in this study were fresh semen from Boer Goat, tris yolk extender, streptomycin, and sweet orange essential oil. Tris yolk extender is made using Tris (hydroxymethylaminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquabides (100 ml). The study design was a non factorial completely treatments given were the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1%. The results showed that the addition of a combination of streptomycin and sweet orange essential oil into the tris yolk extender had a highly significant effect (P <0.01) on the percentage value of spermatozoa abnormalities of Boer Goats before and after freezing. The best average value of spermatozoa abnormalities is the addition of sweet orange essential oil at 1% (P4) with the percentage of spermatozoa abnormalities before freezing at 3% and after freezing at 6%.

scholarly journals The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation

2020 ◽  
Vol 12 (1) ◽  
pp. 125-131
Author(s):  
Raudhah Mahfudhah ◽  
Kartini Eriani ◽  
Zainal Abidin Muchlisin ◽  
Cut Ruhul Muthmainnah
Keyword(s):  
Plasma Membrane ◽  
Clarias Gariepinus ◽  
Membrane Integrity ◽  
Dna Integrity ◽  
Freezing And Thawing ◽  
Randomized Design ◽  
Fetal Bovine ◽  
Fresh Semen ◽  
Different Doses

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.

scholarly journals Utilization Of Frozen Goat Semen With Addition Sweet Orange Essential Oil To Improve Genetic Quality in Ujung Teran Village

2019 ◽  
Vol 1 (2) ◽  
pp. 170-174
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Community Service ◽  
Artificial Insemination ◽  
Sweet Orange ◽  
Genetic Quality ◽  
Know How ◽  
Frozen Semen ◽  
And Training ◽  
Orange Essential Oil

One of the causes of underdeveloped goats is low genetic quality. The way that can be done to improve the genetic quality of goats is to do cross-breeding with superior goats through Artificial Insemination. The community service aims to increase the knowledge of goat farmers in Ujung Teran Village, Langkat Regency on how to make goat frozen semen using tris yolk extender plus sweet orange essential oil for Artificial Insemination program along with the benefits that can be obtained. The method of service is by giving lectures and training to goat farmers. The results obtained from the implementation of this program are that farmers know how to make frozen semen of goat using sweet orange essential oil on the tris yolk extender and are willing to make Artificial Insemination in their goat cattle.

scholarly journals Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation

2019 ◽  
Vol 11 (1) ◽  
pp. 117-124 ◽  
Author(s):  
Yonadiah Dwitya ◽  
Kartini Eriani ◽  
Hendra Saputra ◽  
Al-Azhar Al-Azhar ◽  
Muhammad Rizal
Keyword(s):  
Date Palm ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Phoenix Dactylifera ◽  
Dna Integrity ◽  
Randomized Design ◽  
Sperm Dna ◽  
Completely Randomized Design ◽  
Fresh Semen

Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender.

scholarly journals Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose

2017 ◽  
Vol 9 (3) ◽  
pp. 409
Author(s):  
Kartini Eriani ◽  
Nisa Sari ◽  
Rosnizar Rosnizar ◽  
Dasrul Dasrul ◽  
Suhartono Suhartono ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Membrane Integrity ◽  
Bubalus Bubalis ◽  
Genetic Quality ◽  
Plasma Membrane Integrity ◽  
Randomized Design ◽  
Swamp Buffaloes ◽  
Swamp Buffalo ◽  
Fresh Semen

<p class="IsiAbstrakIndo">Aceh swamp buffaloes<span lang="AR-YE" dir="RTL"> </span><em>(Bubalus bubalis) </em>are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloes<span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">after</span><span lang="AR-YE" dir="RTL"> </span>thawing<em>.</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">This study used completely</span>a factorial randomized design with nine treatments, and five replications. Fresh semen of the Aceh swamp buffalo were diluted by using a combination extender lactose cryoprotectants 0 mM (L<sub>0</sub>), 60 mM (L<sub>60</sub>), 120 mM (L<sub>120</sub>) and glycerol 3% (G<sub>3</sub>), 5% (G<sub>5</sub>), 7% (G<sub>7</sub>) with the equilibration of 4 hours. The results showed that the combination of cryoprotectants L<sub>120</sub>G<sub>7 </sub>influenced significantly (P &lt; 0.05) on the quality of spermatozoa of the Aceh swamp buffalo <em>(B. bubalis)</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">after</span><span lang="AR-YE" dir="RTL">  </span>thawing<em>.</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">The percentage of sperm motility L</span><sub>120</sub>G<sub>7</sub> (42.60 ± 1.14); viability L<sub>120</sub>G<sub>7 </sub>(55.00 ± 0.71);  acrosome integrity L<sub>120</sub>G<sub>7</sub> (52.00 ± 0.71); and plasma membrane integrity L<sub>120</sub>G<sub>7</sub><span lang="AR-YE" dir="RTL"> (</span>53.20 ± 1.48).  The combination of lactose cryoprotectants 120 mM (L<sub>120</sub>) and glycerol 7% (G<sub>7</sub>) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI). </p>

scholarly journals Potential of Flamboyant Flower (Delonix regia (Boj. ex Hook) Raf) Extract to Maintain Post-Dilution Quality of Aceh Cattle (Bos taurus indicus) Spermatozoa

2020 ◽  
Vol 151 ◽  
pp. 01044
Author(s):  
Marlina Muhammad ◽  
Kartini Eriani ◽  
Aida Fithri ◽  
Amalia Rusdi ◽  
Hendra Saputra
Keyword(s):  
Artificial Insemination ◽  
Bos Taurus ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Flower Extract ◽  
Randomized Design ◽  
Bos Taurus Indicus ◽  
Fresh Semen

The objective of this research was to investigate the effect of flamboyant flower extract as a natural antioxidant on sperm quality of Aceh cattle after dilution and equilibration. Fresh semen was collected from 2 healthy bull aged 3.5-4 years old. The research took place in BIB Laboratory. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each. The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The data obtained were analyzed by analysis of variance (ANOVA). Parameters evaluated in this study were motility, viability, plasma membrane integrity, and abnormality. Results showed that there was an insignificant effect (p>0.05) of flamboyant flower extract on the quality of Aceh cattle spermatozoa. The best percentage of motility and viability, 80.0% and 88.4%, respectively, were found at P3 (0.15% flamboyant flower extract). In can be concluded from the research that the addition of 0.15% (m/v) D. regia flower extract was able to maintain sperm quality of Aceh cattle to be used in the artificial insemination program.

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